نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری تغذیۀ طیور، گروه پرورش و مدیریت طیور، دانشکدۀ کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

2 استادیار، گروه پرورش و مدیریت طیور، دانشکدۀ کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

3 استاد، گروه پرورش و مدیریت طیور، دانشکدۀ کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

4 دانشیار، گروه ویروس ‏شناسی، دانشکدۀ دامپزشکی، دانشگاه تهران، تهران، ایران

چکیده

تحقیق حاضر با هدف جداسازی باکتریوفاژ برای مهار زیستی باکتری انتروکوکوس مقاوم به پادزیست و کاهندۀ رشد جداشده از مدفوع بلدرچین ژاپنی در سه آزمایش انجام شد. 60 نمونه از محتویات دستگاه گوارش بلدرچین‌های بالغ برای جداسازی انتروکوکوس‏های بالقوۀ کاهندۀ رشد، جدا و یک نمونۀ منتخب به‌همراه باکتری انتروکوکوس فکالیس سوش51299براساس زنده‌مانی و کاهندگی رشد در بلدرچین‌، انتخاب و در سطح گونه شناسایی شد. سپس باکتریوفاژ لیتیک با استفاده از نمونه‌های فضولات طیور و فاضلاب مرغداری به‌عنوان منابع باکتریوفاژ علیه باکتری منتخب، جداسازی شد. کارایی فاژدرمانی علیه باکتری منتخب در کاهش استقرار باکتری‌های انتروکوکوس در محتویات ایلئوم بلدرچین‌ها متعاقب دریافت دهانی انتروکوکوس بررسی شد. باکتری منتخب به‌عنوان انتروکوکوس فکالیس شناسایی شد. تجویز باکتریوفاژ لیتیک توانست شمار انتروکوکوس‌ها در محتویات ایلئوم را در مقایسه با گروه دریافت‏کنندۀ باکتری و بدون درمان با باکتریوفاژ کاهش دهد و به سطح قابل مقایسه با گروه شاهد منفی (گروه بدون دریافت باکتری انتروکوکوس) برساند (05/0>P). نتایج تحقیق حاضر نشان داد که باکتریوفاژ جداشده می‌تواند به‌طور مؤثر کلونیزاسیون باکتری انتروکوکوس را به‌شکل زیستی مهار کند.

کلیدواژه‌ها

عنوان مقاله [English]

Efficiency of bacteriophage in biocontrol of enterococcal colonization of quail

نویسندگان [English]

  • Jalil Noori Raygani 1
  • Mohammad Amir Karimi Torshizi 2
  • Shaban Rahimi 3
  • Omid Madadgar 4

1 Ph.D. Candidate, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

2 Assistant Professor, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

3 Professor, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

4 Associate Professor, Department of Virology, Faculty of Veterinary, Tehran University, Tehran, Iran

چکیده [English]

Three experiments were carried out for isolation of bacteriophage against growth reducing Enterococcus faecalis and evaluation of its efficacy to control Enterococci colonization in intestine of Japanese quails. In experiment one, 60 Enterococci spp were isolated from gastrointestinal contents of adult quails, which among them seven isolates were selected based on preliminary tests. In experiment two, effect of oral challenge by the seven selected isolates along with Enterococcus faecalis ATCC51299- as positive control on growth performance of growing quails was evaluated. In experiment three, lytic bacteriophage was screened against Enterococcus faecalis using quails excreta and poultry house swage as a sources of phage. Finally, efficiency of bacteriophage therapy (104 pfu/mL) in biocontrol of enterococcal colonization of intestine was evaluated in quails orally challenged by Enterococcus faecalis (107 cfu/mL). Among seven isolates from the first experiment, an isolate which reduced the growth of growing quails and had the minimum mortality in comparison to negative control group (P<0.05) was final selected and identified at the species level using biochemical tests based of carbohydrates fermentation profile as Enterococcus faecalis. Oral administration of isolated bacteriophage against Enterococcus faecalis was able to reduce the intestinal enterococci colonization to a level comparable to negative control group (P<0.05). In conclusion, isolated bacteriophage against Enterococcus faecalis effectively reduced enterococci colonization in intestinal content of quails and in this way, a growth reducing factor in poultry could be biocontrolled.

کلیدواژه‌ها [English]

  • Enterococcus fecalis
  • growth suppressor
  • Japanese quail
  • lytic bacteriophage
  • phage therapy

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