اثر افزودن اسید آلفا-لینولنیک به محیط کشت بلوغ برون‌تنی اووسایت بر توانایی تکوین و کیفیت رویان حاصل از پارتنوژنز و لقاح برون‌تنی در بز

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی گروه علوم دام و طیور، پردیس ابوریحان، دانشگاه تهران

2 دانشیار گروه علوم دام و طیور، پردیس ابوریحان، دانشگاه تهران

3 دانشجوی دکتری گروه علوم دام و طیور، پردیس ابوریحان، دانشگاه تهران

4 استادیار گروه علوم دام و طیور، پردیس ابوریحان، دانشگاه تهران

چکیده

غلظت‌های صفر تا 200 میکرومولار اسید آلفا-لینولنیک (آلفالین) برای مطالعة اثر این اسید چرب بر بلوغ هسته‌ای اووسایت‌ها تعیین شد. اووسایت‌های بز در حضور غلظت‌های صفر (شاهد)، 10، 50، 100 و 200 میکرومولار آلفالین برای بلوغ کشت داده شدند. بیشترین درصد بلوغ برون‌تنی با افزودن 50 میکرومولار آلفالین به محیط کشت اووسایت‌ها حاصل شد (05/0P<). پس از پارتنوژنز/ لقاح برون‌تنی اووسایت‌ها، درصد تسهیم و درصد تولید بلاستوسیست اووسایت‌ها نیز در روزهای سوم و هشتم پس از کشت ثبت شد. میزان آپاپتوز سلول‌های بلاستوسیست نیز ارزیابی شد. پس از پارتنوژنز، درصد تسهیم اووسایت‌هایی که محیط کشت آن‌ها حاوی50 میکرومولار آلفالین بود تفاوتی با شاهد نداشت، ولی درصد تولید بلاستوسیست‌ها در این گروه بالاتر بود (05/0P<). پس از لقاح برون‌تنی، درصد تسهیم و درصد تولید بلاستوسیست گروه 50 میکرومولار آلفالین بیشتر از گروه شاهد بود (05/0P<). همچنین، بلاستوسیست‌های گروه 50 میکرومولار آلفالین، سلول‌های بیشتر و آپوپتوز سلولی کمتری در مقایسه با گروه شاهد داشتند (05/0P<). نتایج این پژوهش نشان داد که اضافه کردن 50 میکرومولار آلفالین به محیط کشت بلوغ برون‌تنی اووسایت‌های بز راندمان بلوغ هسته‌ای، درصد تسهیم، درصد تولید بلاستوسیست و کیفیت آن‌ها را افزایش می‌دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of of α-linolenic acid in maturation medium on developmental competence and quality of blastocysts derived from parthenogenetic activation/fertilization in vitro

نویسندگان [English]

  • Javad Mohammad Moradi 1
  • Ali Akbar Khadem 2
  • Seyed Ahmad Hosseini 1
  • Arash Veshkini 3
  • Ali Asadi Alamouti 4
  • Abdollah Mohammadi-Sangcheshmeh 4
1 B.Sc. Student, Department of Animal and Poultry Science, College of Abouraihan, University of Tehran, Iran
2 Associate Professor, Department of Animal and Poultry Science, College of Abouraihan, University of Tehran, Iran
3 M.Sc. Student, Department of Animal and Poultry Science, College of Abouraihan, University of Tehran, Iran
4 Assistant Professor, Department of Animal and Poultry Science, College of Abouraihan, University of Tehran, Iran
چکیده [English]

In vitro maturation (IVM) was carried out in the presence of different concentrations (10, 50, 100 or 200 µM) of α-linolenic acid (ALA). Embryonic cleavage, blastocyst formation following parthenogenetic activation (PA) and in vitro fertilization (IVF), and numbers of total and apoptotic cells in blastocyst were then determine for the 50 μM concentration and compared with the control group. Out data revealed that ALA increased maturation (MII) rate as compared with control group (P<0.05) and oocytes in 200 μM ALA group showed a lower MII rate as compared with the control group. When oocytes treated with 50 μM ALA were subsequently used for PA or IVF, a higher (P<0.05) rate of blastocyst formation was observed and these embryos had a higher total cell number and a lower apoptotic cell number (P<0.05) as compared with the control group. In conclusion, our results show that supplementation of maturation medium with 50 μM ALA had a positive effect on meiotic maturation by increasing the MII rate and this in turn, stimulated blastocyst formation and also improved quality of the yielded blastocysts.

کلیدواژه‌ها [English]

  • Apoptosis
  • blastocyst
  • Goat oocyte
  • α-linolenic acid
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