نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، دانشکدۀ علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین، خوزستان‌ـ‌ایران

2 استادیار، دانشکدۀ علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین، خوزستان‌ـ‌ایران

3 استاد، گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه شیراز، شیرازـایران

4 استاد، دانشکدۀ علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین، خوزستان‌ـ‌ایران

چکیده

به منظور بررسی تأثیر سطوح متفاوت سیلی‏مارین (صفر، 100، و 200 میلی‏گرم در کیلوگرم جیره) بر عملکرد، خصوصیات لاشه، برخی فراسنجه‏های خونی، و وضعیت آنتی‏اکسیدانی جوجه‏های گوشتی از 120 قطعه جوجۀ گوشتی سویۀ راس 308 در سه تیمار با چهار تکرار و 10 قطعه جوجۀ گوشتی در هر تکرار در قالب طرح کاملاً تصادفی استفاده شد. نتایج آزمایش نشان‌دهندۀ آن است که استفاده از 200 میلی‏گرم سیلی‏مارین، وزن روزانه را در دورۀ آغازین افزایش و در کل دورۀ پرورش کاهش داد و ضریب تبدیل خوراک را در دورۀ آغازین، رشد، و کل دورۀ آزمایش افزایش داد (05/0P<). استفاده از سطوح 100 و 200 میلی‏گرم سیلی‏مارین در جیره، به‌ترتیب بازده لاشه و وزن نسبی سینه را افزایش داد (05/0P<). افزودن 200 میلی‏گرم سیلی‏مارین به جیره باعث افزایش معنی‏دار غلظت مالون‏دی‏آلدهید (05/0P <) و نسبت هتروفیل به لنفوسیت (01/0P<) و کاهش معنی‏دار فعالیت سوپراکسید دسموتاز (05/0P <) شد. براساس نتایج پژوهش حاضر، استفاده از سیلی‏مارین در جیرۀ جوجه‏های گوشتی در شرایط بدون تنش و بیماری، وزن نسبی لاشه و سینه را بهبود داد، ولی بر وضعیت اکسیداتیو پرنده اثری ندارد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of different levels of silymarin on performance, carcass characteristics and antioxidant status in broiler chickens

نویسندگان [English]

  • Rohollah Ebrahimi 1
  • Tahereh Mohammadabadi 2
  • Mohsen Sari 2
  • Somayeh Sallari 2
  • Mohammadjavad Zamiri 3
  • Mohammadtaghi Beigi Nassiri 4

1 Ph.D. Candidate, College of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University, Khouzestan, Iran

2 Assistant Professors, College of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University, Khouzestan, Iran

3 Professor, Animal Reproduction, Animal Science Department, College of Agriculture, Shiraz University, Shiraz, Iran

4 Professor, College of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University, Khuzestan, Iran

چکیده [English]

In order to investigate the effect of different levels of silymarin (0, 100 and 200 mg/kg diet) on
performance, carcass characteristics, blood parameters and antioxidant status, 120 day-old chicks were
assigned to 3 treatments with four replicates and 10 birds in each in a completely randomized design.
Results showed supplementation of 200 mg silymarin significantly decreased the body weight gain
(BWG) in starter (P<0.05) and whole period of experiment (P<0.01) and significantly increased feed
conversion ratio at starter (P<0.05), grower (P<0.05) and total rearing periods (P<0.01). Also, addition of
100 mg silymarin significantly increased the carcass relative weight (P<0.01) and supplementation of 200
mg silymarin significantly increased the breast relative weight (P<0.05). Furthermore, 200 mg silymarin
significantly increased the malondialdehyde and heterophil/ lymphocyte ratio and significantly decreased
superoxide dismutase (P<0.05). These results showed that supplementation of silymarin in diet under
normal condition, improved the carcass and breast relative weight, but no had effect on bird’s oxidative
status.

کلیدواژه‌ها [English]

  • antioxidant status
  • Broiler Chicken
  • carcass characteristics
  • performance
  • Silymarin
1 . اسدی.،ی.،ابوطالب،ن. وشریفیع. م. (1389) بررسی اثر محافظتی (آنتی­اکسیدانی) سیلی مارین برمیزان مرگ سلولی و تولید پراکسیداسیون چربی ناشی از گلوکز بالا در کشت سلول های عصبی PC12. دیابت و لیپید ایران. 9(3): 234-227.
 
2 . Alçiçek A, Bozkurt M and Çabuk M (2004) The effects of a mixture of herbal essential oil, an organic acid or a probiotic on broiler performance. South African Journal of Animal Science. 34: 217-222.
 3 . Barbehenn RV, Poopat U and Spencer B (2003) Semiquinone and ascorbyl radicals in the gut fluids of caterpillars measured with EPR spectrometry. Insect Biochemistry and Molecular Biology. 33(1): 125-130.
4 . Botsoglou NA, Florou-Paneri P, Christaki E, Fletouris DJ and Spais AB (2002) Effect of dietary oregano essential oil on performance of chickens and on iron-induced lipid oxidation of breast, thigh and abdominal fat tissues. British Poultry Science. 43(2): 223-230.
5 . Bouki E, Dimitriadis VK, Kaloyianni M and Dailianis S (2013) Antioxidant and pro-oxidant challenge of tannic acid in mussel hemocytes exposed to cadmium. Marine Environmental Research. 85: 13-20.
6 . Buege J and Aust S (1978) Microsomal lipid peroxidation. Methods Enzymology. 52: 302-310.
7 . Case GL, He L, Mo H and Elson CE (1995) Induction of geranyl pyrophosphate pyrophosphatase activity by cholesterol-suppressive isoprenoids. Lipids. 30: 357-359.
8 . Crowell PL (1999) Prevention and therapy of cancer by dietary monoterpenes. Nutrition. 129(3): 775S-778S.
9 . Emadi M, Kermanshahi H and Maroufyan E (2007) Effect of varying levels of turmeric rhizome powder on some blood parameters of broiler chickens fed corn-soybean meal based diets. Poultry Science. 6(5): 345-348.
10 . Erdogan Z, Erdogan S, Celik S and Unlu A (2005) Effects of ascorbic acid on cadmium-induced oxidative stress and performance of broilers. Biological Trace Element Research. 104(1): 19-32. 
11 . Gross WB and Siegel HS (1983) Evaluation of the heterophil/ lymphocyte ratio as a measure of stress in chickens. Avian Disease. 27(4): 972-979.
12 . Isabel B and Santos Y (2009) Effects of dietary organic acids and essential oils on growth performance and carcass characteristics of broiler chickens. Applied Poultry Research. 18(3): 472-476.
13 . Jamroz D, Wiliczkiewicz A, Wertelecki T, Orda J and Skorupinska J (2005) Use of active substances of plant origin In chicken diets based on maize and locally grown cereals. British Poultry Science. 46(4): 485-493.
14 . Joubert E, Winterton P, Britz TJ and Gelderblom WCA (2005) Antioxidant and pro-oxidant activities of aqueous extracts and crude polyphenolic fractions of rooibos (Aspalathus linearis). Agricultural and Food Chemistry. 53(26): 10260-10267.
15 . Kreeman VSkottová NWalterová DUlrichová J and Simánek V (1998) Silymarin inhibits the development of diet-induced hypercholesterolemia in rats. Planta Medica. 64(2): 138-42.
16 . Kren V and Walterova D (2005) Silybin and silymarin – new effects and applications. Biomedical Papers. 149(1): 29-41.
17 . Lehninger AL (2004) Principles of biochemistry. (4th Ed), W.H. Freeman and company. New York.
18 . Mukarram Shah SM, Khan FA, Shah SMH, Chishti KA, Shah Pirzada SMS, Khan MA and Farid A (2011) Evaluation of Phytochemicals and Antimicrobial Activity of White and Blue Capitulum and Whole Plant of Silybum Marianum. World Applied Sciences. 12(8): 1139-1144.
19 . National Research Council (1994) Nutrient requirement of poultry. 9th review edition. National Academy Press. Washington. D.C.
20 .  Nencini C, Giorgi G and Micheli L (2007) Protective effect of silymarin on oxidative stress in rat brain. Phytomedicine. 14(2-3): 129-135.
21 . Paglia DE and Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Laboratory and Clinical Medicine. 70(1): 158-169.
22 . Peluso MR (2008) Flavonoids attenuate cardiovascular disease. Inhibit phosphodiesterase and modulate lipid homeostasis in adipose tissue and liver. Experimental Biology and Medicine. 231(8): 1287-99.
23 . Pradhan SC and Girish C (2006) Hepatoprotective herbal drug, silymarin from experimental pharmacology to clinical medicine. Indian Journal of Medical Research. 124(5): 491-504.
24 . Quarantelli A, Romanelli S, Basini G and Righi F (2009) The effects of Silymarin on ovarian activity and productivity of laying hens. Italian Journal of Animal Science. 8(2): 769-771.
25 . SAS Institute inc (2001) SAS/STAT Users Guide: Version 9.1. SAS Institute Inc., Cary, North Carolina.
26 . Schiavone A, Righi F, Quarantelli A, Bruni R, Serventi P and Fusari A (2007) Use of Silybum marianum Fruit Extract in Broiler Chicken Nutrition: Influence on performance and meat Quality. Animal Physiology and Animal Nutrition. 91 (5-6): 256-62.
27 . Shaker E, Mahmoud H and Mna S (2010) Silymarin, the antioxidant component and Silybum marianum extracts prevent liver damage. Food and Chemical Toxicology. 48(3): 803-806.
28 . Skottova N and krecman V (1998) Silymarin as a potential hypocholesterolaemic drug. Physiological Research. 47(1): 1-7.
29 . Soto C, Recoba R, Barron H, Alvarez C and Favari L (2003) Silymarin increases antioxidant enzymes in alloxan-induced diabetes in rat pancreas. Comparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology. 136(3): 205-12.
30 . Spencer JPE (2007) The interactions of flavonoids within neuronal signalling pathways. Genes and Nutrition. 2(3): 257-273. 
31 . Tedesco D, Steidler S, Galletti S, Tameni M, Sonzogni O and Ravarotto L (2004) Efficacy of silymarin-phospholipid complex in reducing the toxicity of aflatoxin B1 in broiler chickens. Poultry Science. 83(11): 1839-1843.
32 . Thabrew MI, Hughes RD and McFarlane IG (1998) Antioxidant activity of Osbeckia aspera. Phytotherapy Research. 12(4): 288-290.
33 . Toghyani M, Zarkesh S, Shivazad M and Gheisari A (2007) Immune response of broiler chicks fed chromium picolinate in heat stress condition. Poultry Science. 44(3): 330-334.
34 . Woolliams JA, Wiener G, Anderson PH and Mcmurray CH (1983) Variation in the Activities of Glutathione-Peroxidase and Superoxide-Dismutase and in the Concentration of Copper in the Blood in Various Breed Crosses of Sheep. Research in Veterinary Science. 34(3): 253-256.