نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد، گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه شهید باهنر کرمان

2 دانشیار، گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه شهید باهنر کرمان

چکیده

هدف از انجام پژوهش حاضر، شناسایی جایگاه صفات کمّی (QTL) مرتبط با صفات لاشه روی کروموزوم یک در جمعیت F2 بلدرچین ژاپنی بود. بدین‏ منظور، جمعیتی سه‌نسلی از آمیزش متقابل دو‌سویۀ سفید (تخم‏گذار) و وحشی (گوشتی) بلدرچین ژاپنی ایجاد شد. هشت جفت بلدرچین سفید و وحشی آمیزش داده شد و تعداد 34 پرنده F1 تولید شد. از تلاقی پرندگان F1 تعداد 422 پرنده F2 تولید شد. رکوردهای فنوتیپی وزن اجزای متفاوت لاشۀ پرندگان نسل F2 ثبت شدند. ژنوتیپ همۀ پرندگان هر سه نسل (472) برای هشت نشانگر ریزماهوارۀ موجود روی کروموزوم شمارۀ یک تعیین شد. آنالیز QTL به روش مکان‏یابی درون‌فاصله‏ای مبتنی بر رگرسیون انجام گردید. پس از آنالیز، QTL‏های معنی‏داری برای صفات وزن سینه، وزن لاشه، وزن سر، و درصد سینه شناسایی شد. نتایج نشان داد که یک QTL معنی‏دار با اثر ایمپرینتینگ روی کروموزوم شمارۀ یک وجود دارد که بر وزن سینه به‌عنوان قطعه‌ای با ارزش اقتصادی مؤثر است. واریانس QTL برآورد‌شده در پژوهش حاضر برای QTL‏های با اثرهای افزایشی، غلبه، و ایمپیرینتینگ به‌ترتیب در محدودۀ 8/1–3/2، 2/1–2/2، و 5/0–2/2 درصد بود.

کلیدواژه‌ها

عنوان مقاله [English]

Microsatellite mapping of quantitative trait loci associated with carcass traits on chromosome 1 in Japanese quail

نویسندگان [English]

  • Hasan Moradian 1
  • Ali Esmailizadeh 2
  • Mohammadreza Mohammadabadi 2

1 Graduate M.Sc., Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Iran

2 Associate Professor, Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Iran

چکیده [English]

The purpose of this study was to identify genomic regions of quantitative trait loci (QTL), affecting
carcass traits on chromosome 1 in an F2 population of Japanese quail. For this purpose, a three-generation
resource population was developed by using two distinct Japanese quail strains, wild (meat type) and
white (layer type). Eight pairs of white and wild birds were crossed reciprocally and 34 F1 birds were
produced. The F1 birds were intercrossed to generate 422 F2 offspring. Phenotypic data including weight
of carcass parts were collected on F2 birds. All of the animals from three generations (472 birds) were
genotyped for eight microsatellite markers on chromosome 1. QTL analysis was performed with least
squares interval mapping method fitting three various statistical models. Significant QTL were identified
for breast weight, carcass weight, head weight and percentage of breast. There was also evidence for
imprinted QTL affecting breast weight, a carcass part of high economic value, on chromosome 1. The
proportion of the F2 phenotypic variation explained by the significant additive, dominance and imprinted
QTL effects ranged from 1.8 to 2.3, 1.2 to 3.3 and 0.5 to 2.2 percent, respectively.

کلیدواژه‌ها [English]

  • Carcass traits
  • F2 design
  • Japanese quail
  • microsatellite markers
  • QTL mapping
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