چندشکلی اگزون 10 ژن مربوط به گیرنده هورمون رشد (GHR) و ارتباط آن با صفات رشد در گوسفندان نژاد کرمانی با روش PCR-SSCP*

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، گروه علوم دامی، دانشکده مهندسی علوم دامی و زراعی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج - ایران

2 مربی، گروه علوم دامی، دانشگاه آزاد اسلامی واحد شهربابک، شهربابک - ایران

3 دانش¬آموخته کارشناسی ارشد، گروه علوم دامی، دانشکده مهندسی علوم دامی و زراعی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج - ایران

4 دانشجوی کارشناسی ارشد، گروه علوم دامی، دانشکده مهندسی علوم دامی و زراعی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج - ایران

چکیده

ژن مربوط به گیرنده هورمون رشد ژن هورمون بر روی کروموزوم 16 گوسفند قرار دارند. در این تحقیق، از سیاهرگ وداج تعداد 88 رأس گوسفند موجود در ایستگاه اصلاح نژاد گوسفند کرمانی (شهربابک) خون­گیری شد و DNA نمونه­ها با روش بهینه یافته نمکی استخراج شد و قطعه 155 جفت باز (شامل بخشی از اگزون 10 ژن گیرنده­ هورمون رشد) تکثیر شد. از تفاوت چندشکلی فضایی تک­رشته­ها (SSCP) برای تعیین ژنوتیپ نمونه­ها، استفاده شد. بدین­منظور، الکتروفورز عمودی نمونه­ها روی ژل اکریل­آمید 12 درصد به مدت 17 ساعت و با اختلاف پتانسیل 300 ولت در دمای چهار درجه سانتی­گراد صورت گرفت. همچنین رنگ­آمیزی ژل به روش نیترات نقره انجام شد که طی آن الگوهای ژنوتیپی یک، دو، سه، چهار، پنج و شش به­ترتیب 1/9، 5/4، 6/13، 1/9، 2/35 و 4/28 درصد مشاهده شد. آنالیز واریانس ارقام نشان داد که ارتباط الگوهای متفاوت ژنتیکی با صفات رشد (وزن تولد، وزن سه­ماهگی، وزن شش­ماهگی، وزن نه­ماهگی و وزن 12 ماهگی) معنی­دار نیست.
 

کلیدواژه‌ها


عنوان مقاله [English]

Polymorphism of exon 10 of GHR gene by PCR- SSCP method and its association with growth traits in Kermani sheep breed

نویسندگان [English]

  • H. Moradi Shahrbabak 1
  • A. Asadi 2
  • P. Azizi 3
  • S. Elahian 4
1 - Assistant Professor, Department of Animal Science of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj - Iran (Corresponding Author*)
2 - Instructor, Department of Animal Science of Agricultural Science, Islamic Azad University of Shahrbabak, Shahrbabak - Iran
3 - Former Graduate Student, Department of Animal Science of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj - Iran
4 Graduted Student, Department of Animal Science of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj - Iran
چکیده [English]

Expression of the Growth Hormone receptor (GHR) gene which is located on the sixteenth chromosome of sheep and its binding with GH is essential for growth and fat metabolism. In this study, blood samples were collected from the jugular vein from 88 sheep of Kermani breed.DNA was extracted from blood sample using the modified salting out method for amplification of 155 bp fragment containing a part of exon 10 of GHR genes. Single Strand Conformation Polymorphism (SSCP) was used for genotyping. The vertical electrophoresis of PCR products was performed on 12 percent polyacrylamide gel, at 300 V, for 17 h at 4 ˚C. The silver-staining of gels, resulted identification of six genotypic patterns: 1, 2, 3, 4, 5 and 6 with frequencies of 9.09, 4.54, 13.63, 9.09, 35.22 and 28.40 percent, respectively. Analysis of variance was performed using SAS software and association of different patterns was not significant with growth traits.
 

کلیدواژه‌ها [English]

  • growth traits
  • Growth hormon receptor
  • Kermani sheep
  • PCR-SSCP
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