Polymorphism of exon 10 of GHR gene by PCR- SSCP method and its  association with growth traits in Kermani sheep breed

Moradi Shahrbabak, H.; Asadi, A.; Azizi, P.; Elahian, S.

doi:10.22059/jap.2012.32041

Document Type : Research Paper

Authors

¹ - Assistant Professor, Department of Animal Science of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj - Iran (Corresponding Author*)

² - Instructor, Department of Animal Science of Agricultural Science, Islamic Azad University of Shahrbabak, Shahrbabak - Iran

³ - Former Graduate Student, Department of Animal Science of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj - Iran

⁴ Graduted Student, Department of Animal Science of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj - Iran

https://doi.org/10.22059/jap.2012.32041

Abstract

Expression of the Growth Hormone receptor (GHR) gene which is located on the sixteenth chromosome of sheep and its binding with GH is essential for growth and fat metabolism. In this study, blood samples were collected from the jugular vein from 88 sheep of Kermani breed.DNA was extracted from blood sample using the modified salting out method for amplification of 155 bp fragment containing a part of exon 10 of GHR genes. Single Strand Conformation Polymorphism (SSCP) was used for genotyping. The vertical electrophoresis of PCR products was performed on 12 percent polyacrylamide gel, at 300 V, for 17 h at 4 ˚C. The silver-staining of gels, resulted identification of six genotypic patterns: 1, 2, 3, 4, 5 and 6 with frequencies of 9.09, 4.54, 13.63, 9.09, 35.22 and 28.40 percent, respectively. Analysis of variance was performed using SAS software and association of different patterns was not significant with growth traits.

Keywords

20.1001.1.20096776.1391.14.2.5.8

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Animal Production

Polymorphism of exon 10 of GHR gene by PCR- SSCP method and its association with growth traits in Kermani sheep breed

References

References

Volume 14, Issue 2 - Serial Number 2
January 2012
Pages 43-50

Polymorphism of exon 10 of GHR gene by PCR- SSCP method and its association with growth traits in Kermani sheep breed

References

References

Volume 14, Issue 2 - Serial Number 2January 2012Pages 43-50

Volume 14, Issue 2 - Serial Number 2
January 2012
Pages 43-50