پایش فعالیت های تخمدانی در میش های سنجابی با استفاده از سنجش غلظت پروژسترون

نوع مقاله : مقاله پژوهشی

نویسنده

استادیار،بخش تحقیقات علوم دامی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان کرمانشاه، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرمانشاه، ایران.

چکیده

هدف از انجام این آزمایش، شناسایی فعالیت‌های تخمدانی در طول سال و تعیین طول مدت چرخه‌های فحلی گوسفند سنجابی بود. برای این منظور نمونه‌های سرم خون از 14 رأس میش سنجابی غیر آبستن موجود در ایستگاه تحقیقات دامپروری مهرگان کرمانشاه به‌مدت یک سال و به‌صورت هفتگی و روزانه (21 شهریور تا 20 آبان) جمع‌آوری و با استفاده از روش رادیوایمنواسی غلظت هورمون پروژسترون آنها اندازه‌گیری شد. با بررسی روند تغییرات روزانۀ غلظت پروژسترون خون مشخص شد که در بازهی زمانی 20 شهریور تا 21 آبان ماه، چهار چرخۀ فحلی وجود داشت. میانگین طول چرخه‌های فحلی اول تا چهارم به‌ترتیب 2/41±6/30، 1/90±8/33، 2/50±9/17 و 3/11±16/08 روز و میانگین فاصلۀ زمانی بین چرخه‌های فحلی‌ 2/46±6/47 روز به‌دست آمد. در 61/54 درصد میش‌ها، اوج غلظت پروژسترون (2/02±2/80 نانوگرم در میلی‌لیتر) در ماه آبان ثبت شد و در 38/46 درصد، اوج غلظت پروژسترون (1/14 ± 1/45 نانوگرم در میلی‌لیتر) در فصل بهار، به‌ویژه در فروردین ماه ثبت شد. در ماه‌های خرداد، تیر، بهمن و اسفند به‌ترتیب 78/57، 78/57، 71/43 و 78/57 درصد از میش‌ها در شرایط خاموشی‌جنسی کامل بودند، اما در آبان همۀ میش‌ها چرخۀ تخمدانی فعال داشتند. میانگین طول مدت فعالیت تخمدانی حدود 38±215 روز در سال و در مقابل میانگین طول مدت خاموشی‌جنسی کامل 48±150 روز در سال برآورد ‌شد. نتایج نشان داد میش‌های سنجابی حدود 60 درصد از زمان سال را دارای چرخۀ تخمدانی فعال هستند؛ بنابراین، به‌نظر می‌رسد اجرای برنامه‌های سه بار زایش در دو سال برای این گوسفندان به‌طور طبیعی قابل اجرا باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Monitoring the ovarian activities in Sanjabi ewes using progesterone concentration assay

نویسنده [English]

  • Cyrus Ferasati
Animal Sciences Department, Kermanshah Agricultural and Natural Resources Research and Education Center, AREEO, Kermanshah, Iran.
چکیده [English]

This experiment was conducted to identify the ovarian activity throughout the year and determine the length of estrus cycles of Sanjabi sheep. Accordingly, blood serum samples were collected weekly and daily (12th of September to 11th of November) from 14 non-pregnant Sanjabi ewes at Mehregan livestock research station in Kermanshah for one year and the progesterone concentration was measured using the radioimmunoassay. The daily evaluation of progesterone secretion proceeding showed that there were four estrus cycles during the period from September 11 to November 12. The average length of the first to fourth estrus cycles was 6.30±2.41, 8.33±1.90, 9.17±2.50, and 16.08±3.11 days, respectively, and the mean time interval between estrus cycles was 6.47±2.46 days. The highest progesterone concentration mean in 61.54 percent of ewes was observed in November and in 38.46 percent of ewes was observed in spring, especially in April. In total, 78.57, 78.57, 71.43 and 78.57 percent of the ewes in June, July, February, and March, respectively, were in perfect anestrus conditions, but all of the ewes had the active ovarian cycle in November. The average length of ovarian activities and in contrast perfect anestrus were estimated 215 ± 38 and 150 ± 48 days per year, respectively. The results showed that Sanjabi ewes had an active ovarian cycle about 60 percent of the time of the year; so, there is naturally applicable of a three-time lambing program in two years in this sheep.

کلیدواژه‌ها [English]

  • anestrus period
  • breeding season
  • corpus luteum
  • estrous cycle
  • latitude
  • sheep
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