تأثیر اسید آمینه آرژنین بر صفات کیفی و ترکیب اسید‌های چرب منی در خروس‌های مسن مادر گوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، دانشگاه تهران، پردیس ابوریحان، تخصص: تغذیه طیور

2 دانشیار، گروه علوم دام و طیور، دانشگاه تهران، پردیس ابوریحان

3 دانشیار، گروه علوم دام و طیور - پردیس ابوریحان - تخصص:‌تغذیه طیور

4 استادیار دانشگاه تهران، تخصص: بیوتکنولوژی تولیدمثل

5 استادیار پردیس ابوریحان دانشگاه تهران، تخصص: تعذیه طیور/ مرغ مادر

چکیده

هدف از این مطالعه بررسی تأثیر اسیدآمینه آرژنین بر صفات کیفی و ترکیب اسیدهای چرب اسپرم در خروس­های مسن مادر گوشتی سویه راس 308 بود. به‌همین منظور از تعداد 12 قطعه خروس مادر گوشتی در سن 52 هفتگی، در قالب طرح کاملاً تصادفی با سه تیمار و چهار تکرار به‌مدت هشت هفته استفاده شد. تیمارهای آزمایشی شامل جیره­های با سطوح 0/52 (توصیه راهنمای پرورش سویة راس 308)،0/68 و 0/83 درصد از اسید آمینه آرژنین بود. اسپرم‌گیری از خروس­ها هر 14 روز یکبار و در هفته‌های 54، 56، 58 و 60 آزمایش انجام شد. در پایان دوره نیز از همه خروس‌ها نمونه اسپرم جمع‌آوری و ترکیب اسیدهای چرب اسپرم مورد بررسی قرار گرفت. در هفته‌ 56، حجم منی خروس‌هایی که از تیمار  0/68 درصد آرژنین استفاده کرده بودند، نسبت به دیگر تیمارها بالاتر بود (p<0/05). در هفته 60 سطوح 0/52 و 0/68 درصد آرژنین؛ حجم منی، درصد جنبایی کل و پیش‌رونده بالاتری نسبت به سطح 0/83 درصد آرژنین داشت (p<0/05). در هفته 58، درصد اسپرم‌های با ریخت‌شناسی ناهنجار در خروس‌هایی که از تیمار 0/68 و 0/83درصد آرژنین استفاده کرده بودند نسبت به گروه 0/52 درصد آرژنین کمتر بود (p<0/05). غلظت منی، فعالیت غشای پلاسمایی و ترکیب اسیدهای چرب تحت تأثیر تیمارهای مورد استفاده در این آزمایش قرار نگرفتند. بر اساس نتایج حاصل، استفاده از 0/68 درصد آرژنین (30 درصد بیش­تر از نیاز) در جیره، برخی فراسنجه‌های کیفی اسپرم  در خروس‌های مسن گله مادر گوشتی بهبود می‌بخشد.


کلیدواژه‌ها


عنوان مقاله [English]

The effect of dietary supplementation of l-arginine on qualitative characteristics and fatty acid profile of aged broiler breeder roosters’ sperm

نویسندگان [English]

  • Behnam Abbaspour 1
  • seyed davood sharifi 2
  • shokoufe ghazanfari 3
  • abdollah mohammadi sang cheshmeh 4
  • Shirin Honarbakhsh 5
1 Department of Animal and Poultry Science. College of Aburaihan, University of Tehran
3 Department of Animal and Poultry Science. College of Aburaihan, University of Tehran
4 Department of Animal and Poultry Science. College of Aburaihan, University of Tehran
5 Department of Animal and Poultry Science. College of Aburaihan, University of Tehran
چکیده [English]

This study aimed to evaluate the effect of L-arginine on qualitative characteristics and fatty acid profile of Ross 308 aged broiler breeder roosters’ sperm. Twelve breeder roosters at age of 52 week were used in a completely randomized design with three treatments and four replicates in each treatment for eight consecutive weeks. Experimental groups were consisting diets with levels of 0.52 (Ross recommendation 308), 0.68 and 0.83 present of arginine amino acids. Semen collection was performed every 14 days and in weeks 54, 56, 58 and 60 were tested. At the end of the trial, fatty acid profile of sperm was also evaluated. On week 56, semen volume in roosters with 0.68 present arginine was higher than other treatments (P<0.05). On week 60, levels of 0.52 and 0.68 percent arginine in semen volume, percentage of  total and progressive motility were higher than the level of 0.83 percent of arginine (P<0.05). On week 58, the percentage of abnormal sperm were lower in 0.68 and 0.83 percent arginine treatment compared to 0.52 percent arginine (P<0.05). Semen concentration, sperm plasma membrane functionality and sperm fatty acid profile were not affected by treatments used in this study. It can be concluded that 0.68 percent of arginine (30% higher than recommendation) of diet improve some qualitative sperm parameters in aged broiler breeder roosters.

کلیدواژه‌ها [English]

  • Morphology
  • Motility
  • Ross recommendation
  • Semen Volume
Ahangar M, Asadzadeh S, Rezaeipour V and Shahneh AZ (2017) Effects of L-Arginine supplementation on semen quality, testosterone concentration and testes histological parameters of Ross 308 breeder roosters. Asian Pacific Journal of Reproduction. 6: 133.
Akhlaghi A, Ahangari YJ, Zhandi M and Peebles ED (2014) Reproductive performance, semen quality, and fatty acid profile of spermatozoa in senescent broiler breeder roosters as enhanced by the long-term feeding of dried apple pomace. Animal reproduction science. 147: 64–73.
Altawash ASA, Shahneh AZ, Moravej H and Ansari M (2017) Chrysin-induced sperm parameters and fatty acid profile changes improve reproductive performance of roosters. Theriogenology. 104: 72–79.
Conquer JA, Martin JB, Tummon I, Watson L and Tekpetey F (2000a) Effect of DHA supplementation on DHA status and sperm motility in asthenozoospermic males. Lipids. 35: 149–154.
Dilger RN, Bryant-Angeloni K, Payne RL, Lemme A and Parsons CM (2013) Dietary guanidino acetic acid is an efficacious replacement for arginine for young chicks. Poultry science. 92: 171–177.
Durape NM (2007) Phytochemicals improve semen quality and fertility. Mortality. 3: 2–4b.
Hammerstedt RH (1993) Maintenance of bioenergetic balance in sperm and prevention of lipid peroxidation: a review of the effect on design of storage preservation systems. Reproduction, fertility and development. 5: 675–690.
Heinzel B, John M, Klatt P, Böhme E and Mayer B (1992) Ca2+/calmodulin-dependent formation of hydrogen peroxide by brain nitric oxide synthase. Biochemical Journal. 281: 627–630.
Kelso KA, Cerolini S, Noble RC, Sparks NHC and Speake BK (1997) The effects of dietary supplementation with docosahexaenoic acid on the phospholipid fatty acid composition of avian spermatozoa. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 118: 65–69.
Kelso KA, Cerolini S, Speake BK, Cavalchini LG and Noble RC (1997) Effects of dietary supplementation with α-linolenic acid on the phospholipid fatty acid composition and quality of spermatozoa in cockerel from 24 to 72 weeks of age. Journal of Reproduction and Fertility. 110: 53–59.
Leal A, Caldas-Bussiere MC, de Carvalho CSP, Viana KS and Quirino CR (2009) Role of nitric oxide on quality of freshly ejaculated bull spermatozoa during heparin-induced in vitro capacitation. Animal reproduction science. 116: 38–49.
Lee HJ, Fillers WS and Iyengar MR (1988) Phosphocreatine, an intracellular high-energy compound, is found in the extracellular fluid of the seminal vesicles in mice and rats. Proceedings of the National Academy of Sciences. 85: 7265–7269.
Lefièvre L, Chen Y, Conner SJ, Scott JL, Publicover SJ, Ford WCL and Barratt CLR (2007) Human spermatozoa contain multiple targets for protein S‐nitrosylation: An alternative mechanism of the modulation of sperm function by nitric oxide? Proteomics. 7: 3066–3084.
Lewis SEM, Donnelly ET, Sterling ESL, Kennedy MS, Thompson W and Chakravarthy U (1996) Nitric oxide synthase and nitrite production in human spermatozoa: evidence that endogenous nitric oxide is beneficial to sperm motility. Molecular Human Reproduction. 2: 873–878.
Meyer LE, Machado LB, Santiago APSA, da-Silva WS, De Felice FG, Holub O, Oliveira MF and Galina A (2006) Mitochondrial creatine kinase activity prevents reactive oxygen species generation antioxidant role of mitochondrial kinase-dependent ADP re-cycling activity. Journal of Biological Chemistry. 281: 37361–37371.
Ommati MM, Zamiri MJ, Akhlaghi A, Atashi H, Jafarzadeh MR, Rezvani MR and Saemi F (2013) Seminal characteristics, sperm fatty acids, and blood biochemical attributes in breeder roosters orally administered with sage (Salvia officinalis) extract. Animal production science. 53: 548–554.
Ren B, Cheng X, Wu D, Xu SY, Che LQ, Fang ZF, Lv G, Dong HJ and Lin Y (2015) Effect of different amino acid patterns on semen quality of boars fed with low-protein diets. Animal reproduction science. 161: 96–103.
Rosselli M, Keller RJ and Dubey RK (1998) Role of nitric oxide in the biology, physiology and pathophysiology of reproduction. Human Reproduction Update. 4: 3–24.
Santiago-Moreno J, Castaño C, Coloma MA, Gómez-Brunet A, Toledano-Díaz A, López-Sebastián A and Campo JL (2009) Use of the hypo-osmotic swelling test and aniline blue staining to improve the evaluation of seasonal sperm variation in native Spanish free-range poultry. Poultry science. 88: 2661–2669.
Sarabia Fragoso J, Pizarro Díaz M, Abad Moreno JC, Casanovas Infesta P, Rodriguez‐Bertos A and Barger K (2013) Relationships between fertility and some parameters in male broiler breeders (body and testicular weight, histology and immunohistochemistry of testes, spermatogenesis and hormonal levels). Reproduction in Domestic Animals. 48: 345–352.
Schmidt A, Marescau B, Boehm EA, Renema WKJ, Peco R, Das A, Steinfeld R, Chan S, Wallis J and Davidoff M (2004) Severely altered guanidino compound levels, disturbed body weight homeostasis and impaired fertility in a mouse model of guanidinoacetate N-methyltransferase (GAMT) deficiency. Human molecular genetics. 13: 905–921.
Sharma AC, Lee LY, Hales DB, Law WR, Ferguson JL and Bosmann HB (1998) Effect of NG-nitro-L-arginine methyl ester on testicular blood flow and serum steroid hormones during sepsis. Shock (Augusta, Ga). 9: 416–421.
Stubbs CD and Smith AD (1984) The modification of mammalian membrane polyunsaturated fatty acid composition in relation to membrane fluidity and function. Biochimica et Biophysica Acta (BBA)-Reviews on Biomembranes. 779: 89–137.
Tapeh RS, Zhandi M, Zaghari M and Akhlaghi A (2017) Effects of guanidinoacetic acid diet supplementation on semen quality and fertility of broiler breeder roosters. Theriogenology. 89: 178–182.
Wallimann T, Tokarska-Schlattner M, Neumann D, Epand RM, Epand RF, Andres RH, Widmer HR, Hornemann T, Saks V and Agarkova I (2007) The phosphocreatine circuit: molecular and cellular physiology of creatine kinases, sensitivity to free radicals, and enhancement by creatine supplementation. Mol. Syst. Bioenerg. Energy Life. 195–264.
Zaniboni L, Rizzi R and Cerolini S (2006) Combined effect of DHA and α-tocopherol enrichment on sperm quality and fertility in the turkey. Theriogenology. 65: 1813–1827.