اهمیت اثرات مادری در تنظیم پاسخ شاخص‌های التهابی و الگوی بیان ژن‌های TNF-α و Zo-1 در بافت روده و یا کبد جوجه‌های گوشتی سویه راس 308

نوع مقاله : مقاله پژوهشی

نویسندگان

1 عضو هیات علمی/ دانشگاه زابل

2 دانش آموخته کارشناسی ارشد، دانشگاه زابل

3 دانش آموخته کارشناسی ارشد- دانشگاه زابل

چکیده

در این پژوهش، اثرات تزریق زرده تخم‌مرغ خزک به تخم‌مرغ سویه تجاری راس 308 بر برخی شاخص‌های التهابی و ایمنی، و نیز بیان نسبی ژن‌های TNF-α و یا Zo-1 در بافت روده و کبد نتاج بررسی شد. بدین منظور تعداد 250 تخم‌مرغ بارور سویه راس 308 بطور تصادفی به دو گروه آزمایشی یکسان شامل گروه آزمون (تزریق زرده خزک) و گروه شاهد (تزریق زرده راس) تخصیص داده شدند. پس از تفریخ، جوجه‌ها در شرایط یکسان استاندارد تحت برخی چالش‌های التهابی در سنین 28-21 روزگی برای مدت شش هفته پرورش یافتند. نمونه‌های خون و یا بافت پرندگان در روزهای 10 و 42 جمع‌آوری و برای صفات مورد نظر بررسی شدند. نتایج نشان داد که ترکیبات زرده خزک سبب کاهش بیان سیتوکین التهابی TNF-α در بافت‌های کبد و روده نتاج شد )05/0>(P. در مقایسه با گروه شاهد، تزریق زرده خزک سبب افزایش میزان تیتر آنتی‌بادی طبیعی IGA و تیتر آنتی‌بادی‌های اولیه و ثانویه بر ضد گلبول قرمز گوسفندی (SRBC) و کاهش غلظت سرمی پروتئینCRP و آنزیم کبدی ALT در نتاج شد )05/0>(P. بر اساس نتایج این پژوهش تزریق زردة تخم‌مرغ‌های بومی خزک به زرده تخم‌مرغ‌های سویة تجاری راس 308 می‌تواند به طور مؤثری سبب کاهش میزان بیان سیتوکین‌ التهابی TNF-α در بافتهای کبد و روده نتاج ‌شود.

کلیدواژه‌ها


عنوان مقاله [English]

Importance of maternal effects in the regulation of response of inflammatory markers and expression pattern of TNF-α and Zo-1 genes in intestine and/or liver of Ross 308 broiler chickens

نویسندگان [English]

  • mehdi Vafaye valleh 1
  • nahid Karimi zandi 2
  • Farzaneh Bazmandegan Shomeyli 3
1 Mehdi Vafaye Valleh. Department of Animal Science, Faculty of Agriculture, University of Zabol, P. O. Box 98615–538, Zabol, Iran
2 ندارد
3 ندارد
چکیده [English]

In this study, effects of the in ovo injection of Khazak egg yolk into the yolk of the Ross 308 eggs on some of offspring's inflammatory and immune indices as well as on the relative expression of intestinal and hepatic TNF-α and/or Zo-1 genes, were investigated. For this purpose, 250 fertile Ross 308 eggs were randomly assigned into two equal experimental groups including test (In ovo injection of Khazak yolk) and control (In ovo injection of Ross yolk) group. After hatching, chickens were reared for six weeks under the same standard environmental conditions with exposure to some certain inflammatory stimuli between 21-28 days of age. Chicken’s blood and/or tissues samples were collected on days 10 and 42, and the samples were analyzed for the target traits. Results showed that Khazak yolk component caused a reduction in the levels of pro-inflammatory TNF-α cytokine in both offspring's liver and intestinal tissue (P<0.05). Compared to the control group, khazak yolk injection was found to enhance the titer of IgA natural antibodyas well as primary and secondary antibody titer response against sheep red blood cells (SRBC) and reduces both serum levels of offspring's CRP protein and liver ALT enzyme (P<0.05). According to the results of the present study, injection of Khazak native hen egg-yolk into the yolk of eggs from Ross 308 commercial broiler breeder can effectively suppress the expression of TNF-α inflammatory cytokine in the offspring's liver and intestinal tissue.
 

کلیدواژه‌ها [English]

  • egg yolk
  • Immunity
  • Intestinal inflammation
  • Khazak chicken
  • Natural antibody
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