بررسی چندشکلی ژن‌های‌ Wnt10a و Wnt10b در گوسفند آمیخته افشاری برولامرینو

نوع مقاله : مقاله پژوهشی

نویسندگان

1 --

2 دانشگاه زنجان- دانشیار

3 دانشگاه زنجان- استادیار

چکیده

انتخاب دام­های زنده دارای حداقل چربی لاشه در جهت اصلاح گله­های داشتی به افزایش تولید گوشت کشور کمک می­کند. اعضای خانواده ژن Wnt از عوامل مؤثر در تمایز سلو­ل­های چربی هستند. این پژوهش بهمنظور بررسی ارتباط چندشکلی ژن­های Wnt10a و Wnt10b با صفات لاشه در 96 رأس گوسفند آمیخته افشاری-برولامرینو انجام شد. از نمونه خون گوسفندان آمیخته بهروش فنول کلروفرم DNA استخراج شد و واکنش زنجیره­ای پلی­مراز برای تکثیر قطعه­های 663 جفت­بازی از اگزون سوم و قسمتی از اینترون دوم ژن  Wnt10aو 512 جفت­بازی از اگزون سوم ژن Wnt10b انجام شد. یک چندشکلی و سه تک­شکلی (مشاهده آللی متفاوت از توالی رفرنس) در جایگاه ژن Wnt10a مشاهده شد اما تفاوتی بین نمونهها برای ژن Wnt10b وجود نداشت. یک تک­شکلی در ناحیه اینترون و یک تغییر تکنوکلئوتیدی از نوع هم­معنی و دو تک­شکلی در ناحیه اگزون درژن Wnt10a مشاهده شد که یکی از این تک­­شکلیها در کدون 139بود. پس از هضم با آنزیم برشی HpaII در کدون 139، مشخص شد که تمامی افراد در مقایسه با توالی رفرنس دارای ژنوتیپ هموزیگوت موتانت هستند. این SNP علاوه بر گوسفندان آمیخته در تعدادی از گوسفندان افشاری (دنبه­دار) و زل (بدون دنبه) نیز بررسی شد و مجدداً همان نتایج مشاهده شد. براساس نتایج این تحقیق بهنظر می­رسد در این جایگاه از ژن در نژادهای مورد بررسی آلل اصلی G باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Investigationof the Wnt10a and Wnt10b gene polymorphism in Afshari × Booroola Merino crossbred sheep

نویسندگان [English]

  • Somayeh Zamani afshar 1
  • Taher harkinezhad 2
  • abas Bahari 3
  • mohamad hossein shahir 2
1 --
2 Professor
3 Professor
چکیده [English]

Selection of live animals with minimum carcass fat in animal breeding programs will lead to increase in meat production at national level. The ligands of Wnt genes are of the effective factors in adipocyte cell differentiation. This study was aimed to assess the association between polymorphisms in Wnt10a and Wnt10b genes and carcass traits in 96 Afshari – Booroola Merino male lambs. In this study, DNA was extracted from blood samples using phenol-chloroform extraction method and polymerase chain reaction was performed for amplification a 663 bp fragment of exon III and a part of the second intron of Wnt10a gene and a 512 bp fragment of exon III Wnt10b gene. The results showed a polymorphism and three monomorphisms (a different allele compared to reference sequence of the gene) in the Wnt10a gene but all the sequences of the Wnt10b fragment were the same in studied region of the gene. Results of the sequencing lead to identification of four single nucleotide changes in Wnt10a gene in the studied area when compared to the reference sequence. One of the monomorphisms was in the intron and among other three nucleotide changes identified in exon III one was a missense in codon 139. After digestion with restriction enzymes HpaII at this codon, it was observed that all sampled lambs had mutant homozygous genotype compared to the reference sequence. This site was also evaluated in a number of Afshari (fat- tailed) and Zell (with the least fat tail) but again the same results were observed. Therefore, it seems that in this position the G is the wild type allele in the studied population.

کلیدواژه‌ها [English]

  • Afshari sheep
  • Carcass traits
  • Polymorphism
  • SNP
  • Wnt gene
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