نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی فارغ التحصیل/دانشگاه زابل
2 عضو هیات علمی/ دانشگاه زابل
چکیده
به منظور ارزیابی اثرات مادری بر تکوین سیستم ایمنی نتاج، اثر تزریق زرده تخممرغهای بومی خزک به زرده تخممرغهای سویه تجاری راس بر عملکرد، ایمنی و بیان ژن TLR4 در مرغهای سویه تجاری راس تعیین شد. بدین منظور تعداد 150 عدد تخممرغ سویه راس به طور تصادفی به دو گروه آزمایشی 75 تایی که شامل گروه I (گروه شاهد - تزریق زرده سویه راس) و گروه II (تزریق زرده نژاد خزک) تقسیم و برای مدت سه هفته در در دستگاه جوجه کشی خوابانده و پس از هچ با جیره متوازن به مدت شش هفته پرورش داده شدند. سه پرنده به طور تصادفی در روزهای 27 و 42 انتخاب و کشتار شدند و وزن اندام‌های مؤثر در ایمنی (کبد، بورس و تیموس) اندازه‌گیری شدند. تیتر آنتی‌بادی علیه ویروس نیوکاسل و بیان نسبی ژن TLR4 در کبد نمونه‌های آزمایشی بررسی شد. تزریق زرده خزک به تخممرغهای سویه راس سبب بهبود عملکرد رشد، پاسخ ایمنی و نیز سبب کاهش بیان ژن TLR4 در کبد جوجه‌ها در مقایسه با گروه شاهد شد (05/0P<). بر اساس نتایج این پژوهش ممکن است تزریق زرده تخممرغ های بومی خزک به تخم مرغ های سویه تجاری راس در روز اول جنینی، سبب بهبود کارائی سیستم ایمنی مرغان سویه راس در سنین بالاتر بشود.
کلیدواژه‌ها
عنوان مقاله [English]
The effects of in-ovo injection of Native Khazak egg yolk into the Ross eggs yolk on performance, immunity and expression of TLR4 gene
نویسنده [English]
- Farzaneh Bazmandegan 1
چکیده [English]
To examine the possible role of maternal effect on progeny immune system development, the injection effects of the khazak yolk into the yolk of the commercial Ross egg on the performance, immunity and expression of TLR4 was determined in the commercial Ross chicken. For this purpose, 150 ROSS fertile eggs were randomly assigned to two experimental groups including group I (control-in ovo Ross yolk injection) and group II (in ovo khazak yolk injection) and were kept in incubator for 3 weeks. After incubation period, newly hatched chickens were fed with balanced ration for six weeks. Three Chickens from each experimental group were respectively killed at 27- and 42-d posthatch for analysis of immune organs weight (liver, burs and thymus), The HI anti- body titer levels and liver TLR4 mRNA expression levels. The results of Statistical analysis demonstrated that the in-ovo injection of khazak yolk into the Ross eggs not only significantly enhances growth rate and immune function but also decreases expression of TLR4 mRNA in the liver of treated group compared with the control group (P˂0.05). On the basis of these results it's possible that injection of khazak yolk into the Ross eggs at the first day of embryonic development enhances the chicken immune response at older ages.
کلیدواژه‌ها [English]
- egg yolk
- Khazak native chickens
- Maternal Effects
- TLR4
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