نوع مقاله : مقاله پژوهشی

نویسندگان

1 نویسنده مسئول، مؤسسه تحقیقات علوم دامی کشور، کرج، ایران. رایانامه: mohammadi_v@ut.ac.ir

2 گروه علوم دامی، دانشکده فناوری کشاورزی ابوریحان، دانشگاه تهران، تهران، ایران. رایانامه: shghazanfari@ut.ac.ir

3 گروه علوم دامی، دانشکده فناوری کشاورزی ابوریحان، دانشگاه تهران، تهران، ایران. رایانامه: amohammadis@ut.ac.ir

10.22059/jap.2026.408531.623893

چکیده

هدف: ریزمغذی‌ها، به‌ویژه عنصر روی، نقش مهمی در متابولیسم طبیعی و رشد جوجه‌های گوشتی دارند. استفاده از فناوری‌های نوین به سنتز کمپلکس‌های جدید روی کمک می‌کند تا این ریزمغذی را به‌طور مؤثرتری به بدن برساند. این مطالعه با هدف ارزیابی اثرات منابع معدنی، آلی و نانو عنصر روی بر عملکرد، فراسنجه‌های بیوشیمیایی و ایمنی و کیفیت گوشت جوجه‌های گوشتی انجام شد.
روش پژوهش: به‌ همین منظور 200 قطعه جوجه یک روزه سویه راس 308 به‌صورت تصادفی به پنج تیمار و هر تیمار دارای چهار تکرار تقسیم شدند. گروه‌های آزمایشی به‌ترتیب با جیره بر پایه ذرت و کنجاله سویا (شاهد بدون روی)، جیره پایه مکمل شده با سولفات‌روی، روی متیونین، نانوسولفات‌روی و نانوروی متیونین در سطح 40 میلی‌گرم بر کیلوگرم خوراک تنظیم شد. پرندگان به‌صورت آزاد به آب و خوراک دسترسی داشتند. درجه حرارت و رطوبت نسبی در محدوده مناسب حفظ شد. فراسنجه‌های موردآزمون در این مطالعه، عملکرد تولیدی، خصوصیات لاشه، هماتولوژی خون و کیفیت گوشت ران بود. کمپلکس‌های نانوروی نیز بر اساس تکنولوژی نانوکیلات طراحی شدند.
یافته‌ها: نتایج این مطالعه نشان داد که در پایان دوره آغازین (10 روزگی) کم‌ترین میزان مصرف خوراک در پرندگان تغذیه‌شده با مکمل نانو سولفات‌روی (40 میلی‌گرم بر کیلوگرم) مشاهده شد (05/0>P). پایین بودن مصرف خوراک در این گروه، منجر به کاهش رشد پرندگان در این دوره نیز شد، به‌طوری‌که در مقایسه با سایر گروه‌ها تفاوت قابل‌توجهی داشت (05/0>P). یک تمایل به معنی‌داری در کل دوره (یک تا 42 روزگی) در افزایش وزن بدن بین تیمارها مشاهده شد (06/0P=). پایین‌ترین ضریب تبدیل خوراک در پرندگان تغذیه‌شده با نانوروی متیونین مشاهده شد که به لحاظ آماری با گروه شاهد و نانو سولفات‌روی تفاوت داشت (05/0>P). بازده لاشه در پرندگان تغذیه‌شده با مکمل نانو سولفات‌روی و گروه شاهد کم‌تر از سایر تیمارها بود (05/0>P). وزن نسبی چربی محوطه شکمی در پرندگان گروه شاهد و تغذیه‌شده با مکمل نانو سولفات‌روی نسبت به سایر تیمارها بالاتر بود (05/0>P). درصد لنفوسیت‌های خون در پرندگان تغذیه‌شده با مکمل نانوروی متیونین و روی متیونین پایین‌تر بود (05/0>P). غلظت مالون‌دی‌آلدهید در گوشت ران پرندگان گروه شاهد در زمان‌های 50، 100 و 150 دقیقه در مقایسه با بقیه پرندگان بالاتر بود (05/0>P).
نتیجه‌گیری: به‌طورکلی نتایج مطالعه حاضر نشان داد که گنجاندن 40 میلی‌گرم از منابع روی متیونین (نانو یا آلی) در کیلوگرم خوراک، اثرات مثبتی بر عملکرد رشدی و برخی فراسنجه‎های ایمنی و کیفیت گوشت دارد.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of mineral, organic and Zinc nano-sources on performance, blood biochemical, and immune parameters and meat quality of Broiler chickens

نویسندگان [English]

  • vahid mohammadi 1
  • Shokoofeh Ghazanfari 2
  • Abdullah Mohammadi Sang-Cheshmeh 3

1 Corresponding Author, National Institute of Animal Sciences Research Karaj, Iran. E-mail: mohammadi_v@ut.ac.ir

2 Department of Animal and Poultry Sciences, Faculty of Agricultural Technology(Aburaihan), University of Tehran, Tehran, Iran. E-mail: shghazanfari@ut.ac.ir

3 Department of Animal and Poultry Sciences, Faculty of Agricultural Technology(Aburaihan), University of Tehran, Tehran, Iran. E-mail: amohammadis@ut.ac.ir

چکیده [English]

Objective: Trace elements, particularly zinc (Zn), play a pivotal role in the metabolic processes and growth of broiler chickens. Recent technological advancements have facilitated the synthesis of novel Zn complexes engineered for enhanced nutrient delivery. Consequently, this study was designed to evaluate the effects of various dietary Zn sources on growth performance, carcass characteristics, immunological parameters, and the lipid oxidative stability of broiler meat.
Method: A total of 200 one-day-old Ross 308 broiler chicks were randomly distributed into five experimental treatments, each with four replicates. The treatments consisted of a basal corn-soybean meal diet (Control) or the basal diet supplemented with 40 mg/kg of Zn-sulfate, Zn-methionine, Zn-nano sulfate, or Zn-nano methionine. Feed and water were provided for ad libitum consumption. Environmental temperature and relative humidity were strictly regulated within optimal ranges. Assessments included growth performance, carcass traits, hematological profiles, and thigh meat quality. The nano-complexes were engineered utilizing nano-chelate technology.
Results: At the end of the starter period, the lowest feed intake (FI) was observed in birds supplemented with Zn-nano sulfate (P<0.05). This reduced FI resulted in diminished growth during this phase, showing a significant difference compared to the other groups (P<0.05). Over the entire experimental period (days 1 to 42), a numerical trend toward increased body weight gain (BWG) was noted among treatments (P=0.06). The most favorable feed conversion ratio (FCR) was recorded in the Zn-nano methionine group, which differed significantly from the Control and Zn-nano sulfate groups (P<0.05). Carcass yield was significantly affected by the treatments (P<0.05), with the lowest yields observed in the Control and Zn-nano sulfate groups. Furthermore, the relative weight of the abdominal fat pad was significantly higher in the Control and Zn-nano sulfate groups compared to other treatments (P<0.05). Dietary Zn sources also significantly influenced serum lymphocyte percentages (P < 0.05); the most robust cell-mediated immune response was observed in birds fed Zn-nano methionine and Zn-methionine. Regarding oxidative stability, the extent of lipid oxidation in thigh meat from the Control group was significantly higher at 50, 100, and 150 minutes post-mortem compared to all other treatment groups (P<0.05).
Conclusion: In conclusion, supplementation of broiler diets with 40 mg/kg of nanoscale Zn sources, particularly Zn-nano methionine and Zn-nano sulfate, differentially influenced growth performance, selected immunological traits, and meat quality.

کلیدواژه‌ها [English]

  • Broiler chicken
  • Immune system
  • Lipid oxidation
  • Performance
  • Zinc nanoparticles
Reference
Abd El-Hack, M. E., Alaidaroos, B. A., Farsi, R. M., Abou-Kassem, D. E., El-Saadony, M. T., Saad, A. M., & Ashour, E. A. (2021). Impacts of supplementing broiler diets with biological curcumin, zinc nanoparticles and Bacillus licheniformis on growth, carcass traits, blood indices, meat quality and cecal microbial load. Animals11(7), 1878. https://doi.org/10.3390/ani11071878
Abd El-Hack, M. E., Ashour, E. A., Aljahdali, N., Zabermawi, N. M., Baset, S. A., Kamal, M., ... & Bassiony, S. S. (2024). Does the dietary supplementation of organic nano-zinc as a growth promoter impact broiler's growth, carcass and meat quality traits, blood metabolites and cecal microbiota.  Poultry Science103(5), 103550. https://doi.org/10.1016/j.psj.2024.103550
Abdelnour, S. A., Alagawany, M., Hashem, N. M., Farag, M. R., Alghamdi, E. S., Hassan, F. U., ... & Attia, Y. A. (2021). Nanominerals: fabrication methods, benefits and hazards, and their applications in ruminants with special reference to selenium and zinc nanoparticles. Animals11(7), 1916. https://doi.org/10.3390/ani11071916
Abd-Elsamee, M. O., El-Sherbiny, A. E., Hassan, H. M. A., Samy, A., & Mohamed, M. A. (2012). Adding phytase enzyme to low phosphorus broiler diets and its effect upon performance, bone parameters and phosphorus excretion. doi=ajpsaj.2012.129.137&org=10
Ahmadi, F., Ebrahimnezhad, Y., Sis, N. M., & Ghalehkandi, J. G. (2013). The effects of zinc oxide nanoparticles on performance, digestive organs and serum lipid concentrations in broiler chickens during starter period. doi: doi.org/10.12692/ijb/3.7.23-29
Akhavan-Salamat, H., & Ghasemi, H. A. (2019). Effect of different sources and contents of zinc on growth performance, carcass characteristics, humoral immunity and antioxidant status of broiler chickens exposed to high environmental temperatures. Livestock Science, 223, 76-83. https://doi.org/10.1016/j.livsci.2019.03.008
Al-Daraji, H. J., & Amen, M. H. (2011). Effect of dietary zinc on certain blood traits of broiler breeder chickens. International Journal of Poultry Science, 10(10), 807-813. https://doi.org/10.3923/ijps.2011.807.813
Bakst, M. R., & Akuffo, V. (2007). Alkaline phosphatase reactivity in the vagina and uterovaginal junction sperm-storage tubules of turkeys in egg production: implications for sperm storage. British Poultry Science, 48(4), 515-518. https://doi.org/10.1080/00071660701381761
Cunningham‐Rundles, S., Bockman, R. S., Lin, A., Giardina, P. V., Hilgartner, M. W., CALDWELL‐BROWN, D. O. R. O. T. H. E. A., & Carter, D. M. (1990). Physiological and Pharmacological Effects of Zinc on Immune Response a. Annals of the New York Academy of Sciences, 587(1), 113-122.  https://doi.org/10.1111/j.1749-6632.1990.tb00139.x
de Almeida, M. S., Susnik, E., Drasler, B., Taladriz-Blanco, P., Petri-Fink, A., & Rothen-Rutishauser, B. (2021). Understanding nanoparticle endocytosis to improve targeting strategies in nanomedicine. Chemical society reviews50(9), 5397-5434. DOI: 10.1039/D0CS01127D
Fatholahi, A., Khalaji, S., Hosseini, F., & Abbasi, M. (2021). Nano-Bio zinc synthesized by Bacillus subtilis modulates broiler performance, intestinal morphology and expression of tight junction's proteins. Livestock Science251, 104660. https://doi.org/10.1016/j.livsci.2021.104660
Feng, J. W. Q. M., Ma, W. Q., Niu, H. H., Wu, X. M., Wang, Y., & Feng, J. (2010). Effects of zinc glycine chelate on growth, hematological, and immunological characteristics in broilers. Biological trace element research, 133(2), 203-211. DOI:10.1007/s12011-009-8431-9
Hafez, A., Nassef, E., Fahmy, M., Elsabagh, M., Bakr, A., & Hegazi, E. (2020). Impact of dietary nano-zinc oxide on immune response and antioxidant defense of broiler chickens. Environmental Science and Pollution Research27(16), 19108-19114. DOI: 10.1007/s11356-019-04344-6
Hatab, M. H., Rashad, E., Saleh, H. M., El-Sayed, E. S. R., & Taleb, A. A. (2022). Effects of dietary supplementation of myco-fabricated zinc oxide nanoparticles on performance, histological changes, and tissues Zn concentration in broiler chicks. Scientific Reports12(1), 18791. DOI: 10.1038/s41598-022-22836-3
Huang, Y. L., Lu, L., Luo, X. G., & Liu, B. (2007). An Optimal Dietary Zinc Level of Broiler Chicks Fed a Corn-Soybean Meal Diet1. Poultry Science86(12), 2582. DOI: 10.3382/ps.2007-00088
Kakhki, R. A. M., Bakhshalinejad, R., Hassanabadi, A., & Ferket, P. (2017). Effects of dietary organic zinc and α-tocopheryl acetate supplements on growth performance, meat quality, tissues minerals, and α-tocopherol deposition in broiler chickens. Poultry science96(5), 1257-1267. https://doi.org/10.3382/ps/pew386
Leeson, S., & Caston, L. (2008). Using minimal supplements of trace minerals as a method of reducing trace mineral content of poultry manure. Animal Feed Science and Technology142(3-4), 339-347. https://doi.org/10.1016/j.anifeedsci.2007.08.004
Liu, Z. H., Lu, L., Li, S. F., Zhang, L. Y., Xi, L., Zhang, K. Y., & Luo, X. G. (2011). Effects of supplemental zinc source and level on growth performance, carcass traits, and meat quality of broilers. Poultry Science90(8), 1782-1790. https://doi.org/10.3382/ps.2010-01215
 Marreiro, D. D. N., Cruz, K. J. C., Morais, J. B. S., Beserra, J. B., Severo, J. S., & De Oliveira, A. R. S. (2017). Zinc and oxidative stress: current mechanisms. Antioxidants, 6(2), 24.  https://doi.org/10.3390/antiox6020024
Moghaddam, H. N., & Jahanian, R. (2009). Immunological responses of broiler chicks can be modulated by dietary supplementation of zinc-methionine in place of inorganic zinc sources. Asian-Australasian journal of animal sciences22(3), 396-403. https://doi.org/10.5713/ajas.2009.80473
Mohammadi, V., Ghazanfari, S., Mohammadi-Sangcheshmeh, A., & Nazaran, M. H. (2015). Comparative effects of zinc-nano complexes, zinc-sulphate and zinc-methionine on performance in broiler chickens. British poultry science56(4), 486-493. DOI: 10.1080/00071668.2015.1064093
Mohanna, C., & Nys, Y. (1999). Effect of dietary zinc content and sources on the growth, body zinc deposition and retention, zinc excretion and immune response in chickens. British Poultry Science, 40(1), 108-114. https://doi.org/10.1080/00071669987926
Mohd Yusof, H., Abdul Rahman, N. A., Mohamad, R., Zaidan, U. H., & Samsudin, A. A. (2022). Influence of dietary biosynthesized zinc oxide nanoparticles on broiler zinc uptake, bone quality, and antioxidative status. Animals13(1), 115. https://doi.org/10.3390/ani13010115
Navidshad, B., Mohammadrezaei, M., Zarei, M., Valizadeh, R., Karamati, S., Rezaei, F., & Esmaeilinasab, P. (2019). The New Progresses in Trace Mineral Requirements of Broilers, a Review. Iranian Journal of Applied Animal Science9(1).
NRC. (1994). Nutrient requirements of poultry. Ninth Revised Edition, National Academy Press, Washington DC. 15-18.
O’Connor, J. P., Kanjilal, D., Teitelbaum, M., Lin, S. S., & Cottrell, J. A. (2020). Zinc as a therapeutic agent in bone regeneration. Materials, 13(10), 2211. https://doi.org/10.3390/ma13102211
Ogbuewu, I. P., & Mbajiorgu, C. A. (2023). Potentials of dietary zinc supplementation in improving growth performance, health status, and meat quality of broiler chickens. Biological trace element research201(3), 1418-1431. https://doi.org/10.1007/s12011-022-03223-5
Rao, S. R., Prakash, B., Raju, M. V. L. N., Panda, A. K., Kumari, R. K., & Reddy, E. P. K. (2016). Effect of supplementing organic forms of zinc, selenium and chromium on performance, anti-oxidant and immune responses in broiler chicken reared in tropical summer. Biological trace element research, 172(2), 511-520.  DOI: 10.1007/s12011-015-0587-x
Sagar, P. D., Mandal, A. B., Akbar, N., & Dinani, O. P. (2018). Effect of different levels and sources of zinc on growth performance and immunity of broiler chicken during summer. International Journal of Current Microbiology and Applied Sciences, 7(5), 459-471. https://doi.org/10.20546/ijcmas.2018.705.058
SAS, (2003). SAS/STAT User's Guide, Release 8.02 ed. SAS Institute Inc., Cary, NC, U.S.A.
Shokri, P., Ghazanfari, S., & Honarbakhsh, S. (2021). Effects of different sources and contents of dietary manganese on the performance, meat quality, immune response, and tibia characteristics of broiler chickens. Livestock Science, 253, 104734.   https://doi.org/10.1016/j.livsci.2021.104734
Stahl, J. L., Cook, M. E., Sunde, M. L., & Greger, J. L. (1989). Enhanced humoral immunity in progeny chicks from hens fed practical diets supplemented with zinc.
Toghyani, M., Toghyani, M., Gheisari, A., Ghalamkari, G., & Mohammadrezaei, M. (2010). Growth performance, serum biochemistry and blood hematology of broiler chicks fed different levels of black seed (Nigella sativa) and peppermint (Mentha piperita). Livestock science, 129(1-3), 173-178. https://doi.org/10.1016/j.livsci.2010.01.021
Xie, Y., Liu, F., Zhang, X., Jin, Y., Li, Q., Shen, H., ... & Mao, J. (2022). Benefits and risks of essential trace elements in chronic kidney disease: a narrative review. Annals of Translational Medicine10(24), 1400. doi: 10.21037/atm-22-5969
Zhao, C. Y., Tan, S. X., Xiao, X. Y., Qiu, X. S., Pan, J. Q., & Tang, Z. X. (2014). Effects of dietary zinc oxide nanoparticles on growth performance and antioxidative status in broilers. Biological trace element research, 160(3), 361-367. DOI: 10.1007/s12011-014-0052-2