Ameneh Naseri Moghadam; Mohammad Ebrahim Nooriyan Soroor; Fardin Hozhabri
Abstract
In order to investigate the effect of licorice extract (LE) on growth performance, fermentation parameters, and rumen protozoan population in fattening lambs, 28 male lambs weighing 36.45 ± 1.75 kg in a completely randomized design with four treatments and seven replicates were used. Experimental ...
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In order to investigate the effect of licorice extract (LE) on growth performance, fermentation parameters, and rumen protozoan population in fattening lambs, 28 male lambs weighing 36.45 ± 1.75 kg in a completely randomized design with four treatments and seven replicates were used. Experimental treatmeشnts included: control, basic diet without licorice extract, and treatments one to three included basic diet plus five, 10 and 25 mg of LE per kilogram of DM. The ratio of forage to concentrate was 30:70, which was given to the animals as per their appetite. Adding 25 mg of the extract to the diet increased the daily gain and improved the feed conversion ratio (P<0.05). The pH of rumen fluid and gas production increased at the level of 25 mg of extract (P<0.05). Methane production was not affected by adding LE to the diet. The changes in metabolizable energy, degraded organic matter and concentration of volatile fatty acids in diets containing 5 and 25 mg of LE were more than other groups (P<0.05). The total population of protozoa as well as the number of Entodinia decreased by feeding diets containing LE (P<0.05) but the number of Isotrichida increased, significantly. Based on the results of this research, adding 25 mg/kg of licorice extract to the diet improves the rumen metabolism and the performance of fattening lambs, but it had no effect on the amount of energy loss in the form of methane.
Hamid Amanlou; Hadi Shafiei; Tahereh Amirabadi Farahani; Farshid Kheiri; Najme Eslamian Farsuni
Abstract
In order to compare the precision of predictions of NRC (2001) and CNCPS models, 17 Holstein fresh cows were assigned in a randomized complete block design to a balanced diet with NRC (2001) and CNCPS software. The predicted DMI, energy allowable milk and protein allowable milk by both models were compared ...
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In order to compare the precision of predictions of NRC (2001) and CNCPS models, 17 Holstein fresh cows were assigned in a randomized complete block design to a balanced diet with NRC (2001) and CNCPS software. The predicted DMI, energy allowable milk and protein allowable milk by both models were compared with observed values. The precision of the predictions of models was evaluated by calculation of R2, mean bias and root mean square prediction error (RMSPE). Both models predicted DMI lower than the observed values (P<0.01). The mean bias, RMSPE and R2 were 2.22, 2.84 and 0.48 for NRC (2001) and 3.18, 3.99 and 0.52 for CNCPS, respectively and CNCPS predicted the DMI 20 percent lower than the observed value. The observed milk production was 29.78 kg/d. predicted energy allowable milk by NRC (2001) and CNCPS were 25.68 and 22.32 kg/d, respectively. Predicted protein allowable milk with NRC (2001) and CNCPS were 25.80 and 28.37 kg/d, respectively and NRC (2001) predicted the milk production 14 percent lower than observed value. Given the importance of DMI in fresh cows seems NRC (2001) has more precision compared to CNCPS for estimating nutritional requirements.
hamid marzbani; Hossein Moradi Shahrbabak; Mohammad Moradi Shahre Babak
Abstract
This study was conducted to estimate the linkage disequilibrium (LD) and determine haplotype blocks structure in 93 Sarabi cow using SNP-chip 40k of Illumina company. After genotyping and quality control, 27386 SNP markers on autosomal chromosomes remained for analyzing. The LD was measured by r2 and ...
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This study was conducted to estimate the linkage disequilibrium (LD) and determine haplotype blocks structure in 93 Sarabi cow using SNP-chip 40k of Illumina company. After genotyping and quality control, 27386 SNP markers on autosomal chromosomes remained for analyzing. The LD was measured by r2 and D' statistics. In this study the average of r2 and D' for range less than of 2.5 kb were maximum with 0.505 and 0.927, respectively. The average of r2 and D' were minimum with 0.064 and 0.486, respectively in range of 2-5 Mb. 582 haplotype blocks were observed in the genome of Sarabi cow. 6.73% SNP from all of the SNPs were covered and 0.83% (21.43 Mb) of the autosomal genome were covered by the blocks haplotype. Population effective size was estimated about 40 that refer to four generations ago. The low number of haplotype block and also low LD level in Sarabi cow population showed high variation. In refer to the result and the number of haplotype blocks in this breed, applying the haplotype blocks could be improve results and high precision on genomic selection study so it was recommended that in study of genomic selection applying the haplotype blocks really useful than single SNP study
Hossein Emrani; Rasoult Vaes Torshizi
Abstract
Ascites Syndrome is a costly metabolic disorder that has been linked to intense selection on growth rate and low feed conversion ratio in broilers. This Syndrome has become one of the most frequent causes of mortality within the broiler industry and leads to substantial economic losses and reduced animal ...
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Ascites Syndrome is a costly metabolic disorder that has been linked to intense selection on growth rate and low feed conversion ratio in broilers. This Syndrome has become one of the most frequent causes of mortality within the broiler industry and leads to substantial economic losses and reduced animal welfare. To identify genes and genomic regions associated with Ascites Syndrome, genome wide association study was performed by the chicken 60K SNP panel on 101 birds from a F2 chicken population derived from the reciprocal cross between Arian broiler Line and Azerbaijan native fowls. Genomic best linear unbiased prediction procedure was used to calculate associations between each SNP and ascite related traits. Several SNPsfor the ratio of right ventricular to total ventricular, an indicator for Ascites syndrome, reached the suggestive threshold (8.25×10-5) on chromosome 7. Two SNPs detected within CCDC141 and OSBPL6 genes were previously reported for human heart disorder. There are a large number of genes in this region (12745561-14602723) on chicken chromosome 7 that are related to hypertrophic cardiomyopathy, heart rate and human heart disorder. Selection for ascites resistance in broiler chicken using these findings could accelerate the genetic progress.
mohammad asadi; Taghi Ghoorchi; Abdolhakim Toghdory
Abstract
Introduction: During pregnancy, especially the period around birth, animals make many metabolic adjustments to support the transition from pregnancy to lactation. Since the last two months of pregnancy (late pregnancy) constitute 75% of fetal growth, therefore, enriching the sheep diet can have positive ...
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Introduction: During pregnancy, especially the period around birth, animals make many metabolic adjustments to support the transition from pregnancy to lactation. Since the last two months of pregnancy (late pregnancy) constitute 75% of fetal growth, therefore, enriching the sheep diet can have positive consequences on the health and general performance of the ewe and lamb. In such conditions, using of management and nutritional strategies will reduce the problems during the transition period and heat stress condition. Under stress condition, the mobilization of glycogen reserves and glucose production occur to produce energy to maintain stability in the body. Chromium is one of the scarce essential minerals that plays an important role in the metabolism of carbohydrates, lipids, proteins and nucleic acids. Chromium is part of the glucose tolerance factor that increases the affinity of insulin receptors to insulin hormone and increases the sensitivity of receptors to insulin.
Material and Methods: This experiment was conducted to evaluate the effect of different forms of chromium in ewes during the transition period under the heat stress condition. Forty pregnant Afshari ewes from 42±5 days before the expected lambing were assigned to four treatments with 10 replications in a completely randomized design. Experimental treatments included: 1) basal diet without chromium supplementation (control), 2) basal diet containing three milligrams of inorganic chromium per kg of DM, 3) basal diet containing three milligrams of chromium in the form of chromium-methionine per kg of DM and 4) basal diet containing three milligrams of chromium in the form of chromium nanoparticles per kg of DM.
Results and Discussion: The results showed that ewes receiving chromium had less weight changes at two, four and six weeks after lambing (P≥0.05). Also, supplementation of different forms of chromium increased the body condition score of ewes at the time and six weeks after lambing (P≥0.05). Ewes receiving chromium-methionine and chromium nanoparticles had better body condition than control and inorganic chromium treatments. There was no significant difference in glucose concentration between the treatments 60 and 90 minutes after intravenous glucose injection. After 60, 90, 120 and 180 minutes after the intravenous injection of glucose, the insulin concentration of the ewes receiving different forms of chromium decreased compared to the control treatment (P≥0.05).
Conclusion: In general, the use of chromium, especially in the form of chromium-methionine and chromium nanoparticles, is recommended during the transition period of ewes under the influence of heat stress.
Nasrin Hoseinpour; Mohammad Ali Norouzian; Ahmad Afzalzadeh
Abstract
This study was conducted to investigate effect of different sources of copper on gas production parameters and nutrients digestibility in Zandi male lambs. In the first experiment, 18 male lambs (initial body weight 17.53±1.62) divided into three groups and fed one of the three experimental diets ...
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This study was conducted to investigate effect of different sources of copper on gas production parameters and nutrients digestibility in Zandi male lambs. In the first experiment, 18 male lambs (initial body weight 17.53±1.62) divided into three groups and fed one of the three experimental diets for 70 days. Dietary treatments were control (basal diet without copper supplement), basal diet plus 10 ppm copper from sulfate and basal diet plus 10 ppm from proteinate copper. In comparison with control, copper supplementation improved average daily gain and feed conversion ratio, but there was no significant difference among the dietary treatments for dry matter intake. Dry matter, organic matter, crude protein and acid detergent fiber digestibility was higher for lambs fed with organic copper compared to lambs fed with control and inorganic copper (P<0.01). In the second experiment, an in vitro gas production technique was used to evaluate the effects of copper sources on the amount and rate of gas production, dry matter degradability, utilization of metabolizable energy (ME), and ruminal fermentation patterns using rumen fluid from three lambs as inoculum. Rate of gas production (c) did not differ among the treatments. Asymptotic of gas production (b) was higher for diets containing supplemental copper compared to control. It seems that supplementation of fattening lamb diet with of organic copper could be improved rumen fermentation and performance of lambs.
Elham Javdan; Jamal Fayazi; Saleh Tabatabaei; Mohammadtaghi Baigi Nasiri
Abstract
This study was conducted to detect polymorphism of gene BMP15, a member of Transforming GrowthFactor β (TGFβ) family which has a crucial role in controlling the ovarian follicles development,ovulation rate and fertility. Samples were randomly selected from 91 Najdi goats in 3 geographicallocations, ...
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This study was conducted to detect polymorphism of gene BMP15, a member of Transforming GrowthFactor β (TGFβ) family which has a crucial role in controlling the ovarian follicles development,ovulation rate and fertility. Samples were randomly selected from 91 Najdi goats in 3 geographicallocations, northwest, southeast and center of Khuzestan province. After DNA extraction, amplification of235 bp fragment of exon 2 of BMP15 gene was performed using specific primers. Sequence detectionwas executed after amplification of gene fragments. The association of BMP15 gene and litter size wasdone by SAS software. Results from sequencing were analyzed by Vector NTI software. The resultsidentified three mutations in bases 529 (T to G), 530 (C to G) and 576 (T to C). The largest litter sizebelongs to AA pattern. Point mutations in this gene will alter the Ovulation rate of the goat. Therefore,improving twining trait in Najdi population can be expected by marker assisted selection.
Parvin Sareminejad; Ali Kiani; Ayoub Azizi
Abstract
Introduction The natural behavior of horses is to spend the most of their time foraging. The feeding time of stabled horses is often far from enough to fulfill their natural eating behavior. Hay-net technique is a method aiming to promote natural foraging behavior, slowing ingestion and increasing the ...
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Introduction The natural behavior of horses is to spend the most of their time foraging. The feeding time of stabled horses is often far from enough to fulfill their natural eating behavior. Hay-net technique is a method aiming to promote natural foraging behavior, slowing ingestion and increasing the length of feeding time. This study was conducted to investigate the effects of using a slow-feed (hay-net) on feeding time, forage intake rate, apparent digestibility, volatile fatty acids (VFA) concentration, bacterial population, and the activity of cellulolytic and amylolytic enzymes in the colon of horses.Materials and Methods Eight Arabian horses (body weight= 396± 45 kg, age= 9±3 years) were used in a cross-over design. The length of the experimental period was eight weeks, which included two periods of four weeks in rotation. In each period, feed was provided for four horses freely and for other four horses using a hay net. Fecal samples were collected on days 21 to 28 of each period.Results and Discussion The results showed that using hay net significantly increased feeding time from 257 to 659 minutes per day and decreased forage intake rate from 1592 and 610 g/h (P<0.05). The hay-net did not affect the total VFA and relative VFAs concentrations (P< 0.05). Lactobacillus populations in horses with hay-net increased compared to those without hay-net (P<0.05). The populations of cellulolytic, amylolytic, Escherichia coli, and coliforms bacteria were unchanged by hay-net. The activity of carboxy methyl cellulase enzyme significantly increased (P<0.05), while the activity of microcrystalline cellulase, amylase, and filter paper decomposition activity was not affected by hay-net.Conclusion In comparison with the more traditional methods, slow-feed hay net devices successfully limited the forage intake rate, expanded feeding time, and changed the activity of some fiber-decomposing enzymes in the large intestine of horses; however, the recommendation of hay-nets for nutritional management in horses needs further investigations.
shahla Nematollahian; Masoud Alipanah
Abstract
Different types of imported and domesticatedhybrids of silkworm are rearing in different climatic conditions in Iran. The purpose of this study was to compare the performance of silkworm hybrids used in two climatic regions ofIran, namely Shaft in Guilan province and Torbat Heydariyeh in Khorasan Razavi ...
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Different types of imported and domesticatedhybrids of silkworm are rearing in different climatic conditions in Iran. The purpose of this study was to compare the performance of silkworm hybrids used in two climatic regions ofIran, namely Shaft in Guilan province and Torbat Heydariyeh in Khorasan Razavi province.For this study, four Iranian hybrids and 14 Chinese imported hybrids were used in a completely randomized block design. For the studied traitsnumber of cocoons per liter, average weight of a cocoon, and weight of cocoon produced from a box, there was a significant difference(P<0.01) between the hybrids as well as the regions. The results of the present study showed that the performance of silkworm hybrids, regardless of their origin, is more affected by the rearing environment and there is a difference between hybrids in the studied climates. Therefore, selecting the most suitable hybrids for different climates of Iran seem to be essential.
Sasan ghamari; Farhang Fatehi; Kamran Rezayazdi
Abstract
Introduction: The production of dairy goat breeds such as Saanen has become widespread in many countries and factors like the nutritional value of goat milk, high amounts of milk yield compared to body weight, and less methane gas production have played important role for the mentioned development. Previous ...
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Introduction: The production of dairy goat breeds such as Saanen has become widespread in many countries and factors like the nutritional value of goat milk, high amounts of milk yield compared to body weight, and less methane gas production have played important role for the mentioned development. Previous studies have shown that reaching the insemination weight (35 kg) of replacement female goats is one of the most important points in the breeding of the dairy goat breeds. Subsequently, other studies have shown that improving growth and weight gain during suckling period reduces the time to reach the required weight and body size for insemination, the growth of mammary glands and increases milk production, and it is economically important. Protein is one of the factors which play important role in growth and body frame development of goats especially at life's early stages. Therefore, the aim of the present study was to investigate the effects of different levels of crude protein in milk replacer on performance of Saanen suckling kids.
Material and Methods: Sixteen Saanen female kids with 10 days of age and 4.678± 0.57 kg of BW were randomly assigned to two experimental treatments, including milk replacer with 22% crude protein (22CP; milk replacer contain 22% crude protein) and milk replacer with 28% crude protein (28CP; milk replacer contain 22% crude protein plus 10 gram of whey protein concentrate per liter of milk replacer). The experimental kids were weaned at 64 days of age and during this time milk replacer, starter, and alfalfa hay intake of kids was recorded daily, and the weight of the goats was measured weekly.
Results and Discussion: The results showed that average daily weight gain of kids fed with 28CP treatment was significantly higher (P < 0.05) compared to the 22CP treatment during the first month, the second month and whole experimental period. Also, the kids body weight at the end of second month for 28CP treatment was significantly higher (P = 0.05) compared to the 22CP treatment (16.14 vs. 14.21 kg). The amount of total dry matter intake (DMI) during the entire experimental period was higher (P < 0.05) for the 28CP treatment compared to the 22CP treatment (509.65 vs. 459.68 grams per day). There were no significant differences among the experimental treatments for the feed conversion ratio (FCR) during the first month, the second month and the entire experimental period. In fact, although the 28 CP treatment had a higher daily weight gain compared to the CP22 treatment, due to higher DMI, but it had a similar FCR compared to the CP22 treatment.
Conclusion: Results showed that feeding milk replacer containing high levels of crude protein (28%) can results to the higher average daily gain and subsequently heavier weaning weight in Saanen goat kids.
mohammad ali ahangarani; Mohammadreza Dehghani; abbas ali naserian
Abstract
The aim of this research was to investigate the effect of adding fat powder and flaxseed oil in milk on performance, growth, health, blood and rumen parameters of 45 suckling calves (avarage body weight = 38 ± 0.5 kg) in a compeletly randomized design in 10℃ of average temperature from d 3 to ...
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The aim of this research was to investigate the effect of adding fat powder and flaxseed oil in milk on performance, growth, health, blood and rumen parameters of 45 suckling calves (avarage body weight = 38 ± 0.5 kg) in a compeletly randomized design in 10℃ of average temperature from d 3 to 30 of age in individual boxes. Experimental treatments included: 1- basal starter and milk without fat supplement (control) 2- basal starter and milk with fat powder (10 g/liter of milk) 3- basal starter and milk with flaxseed oil (10 g/liter of milk). Daily weight gain from d 3 to 30 and weight at d 30 were higher in flaxseed oil treatment than control treatment (P ≤ 0.05). Calves fed flaxseed oil treatment had higher hip height than control treatment at d 30 (P ≤ 0.05). Blood cholesterol concentration was higher in control treatment than flaxseed oil treatment and blood cortisol concentration was lower in flaxseed oil treatment than control treatment (P ≤ 0.05). The ratio of total protein to albumin in flaxseed oil treatment was lower than control treatment (P ≤ 0.05). Ruminal parameters were not affected by the experimental treatments. The results of this research indicated that using of fat sources including linolenic acid as flaxseed oil in milk have a positive effect on the performance, growth and health during the first month after birth of dairy calves.
Mahshid Mohammadpanah; Homayoun Farhangfar; Moslem Bashtani
Abstract
Correlation between breeding value of crude milk (CM) and energy-corrected milk (ECM) was studied based on a total of 774,013 test day records belongs to 88,456 first-parity Holsteins dairy cattle calving during 1997-2009 in 165 herds (from six provinces). Genetic analysis of the traits was carried out ...
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Correlation between breeding value of crude milk (CM) and energy-corrected milk (ECM) was studied based on a total of 774,013 test day records belongs to 88,456 first-parity Holsteins dairy cattle calving during 1997-2009 in 165 herds (from six provinces). Genetic analysis of the traits was carried out by a fixed regression test day model. In the model, effects of herd, calving year, production month, production age, type of cow genotype (grade or pure Holstein), sperm type, polynomial function, as well as additive genetic and permanent environmental random effects of the cows were included. Mean breeding values as the genetic evaluation is undertaken based on two traits were statistically significant (P<0.0001). Correlation between breeding value of CM and ECM was the highest (0.92) when all the cows were selected based upon breeding value for ECM and was the lowest (0.27) as 10 top cows were selected based upon breeding value for ECM. It could be therefore concluded that in the case of using ECM instead of CM records, change in the ranking of the elite cows is appreciable.
Mehdi Vafaye Valleh; nahid Karimi zandi; Mahdeieh Najmadini
Abstract
In this research, the effect of in ovo injection of egg yolk of native kahazak chicken into the egg yolk of the commercial Ross 308 strain at prior to incubation on some metabolic-related parameters were tested in the resulting progeny. The search parameters included feed intake, feed conversion ratio, ...
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In this research, the effect of in ovo injection of egg yolk of native kahazak chicken into the egg yolk of the commercial Ross 308 strain at prior to incubation on some metabolic-related parameters were tested in the resulting progeny. The search parameters included feed intake, feed conversion ratio, some of serum biochemical parameters, malondialdehyde concentration as well as relative expression of AMP-activated protein kinase (AMPK) and Neuropeptide Y (NPY) genes in progeny's brain tissue. To achieve this goal, 320 fertile Ross 308 eggs were equally allocated in a completely randomized design into two experimental groups, those injected with Khazak yolk (Test) and control group. Blood and tissue samples were collected at the end of experiment and evaluated for the parameters noted. Compared with the control group, khazak egg-yolk injection resulted in a significant improvement in the feed conversion ratio as well as significant decreases in serum triglyceride, triglyceride/HDL-C ratio, relative visceral fat mass content and levels of AMPK expression in the progeny's brain. In contrast, Khazak yolk injection had no effect on the 45-day body weight, cumulative feed intake, relative brain weight, serum glucose, cholesterol and LDL-c levels but also had no effects on malondialdehyde concentration and NPY expression in progeny brain tissue. According to the results of this study, the injection of native Khazak egg yolk into the yolk of the commercial Ross 308 eggs have effectively improved the feed efficiency and reduced AMPK expression in the diencephalon region of the progeny's brain.
mahdi dehghani-sanij; Mohammad Ali Norouzian; Ahmad Afzalzadeh; ali assadi-alamouti
Abstract
To study the effects of feeding rumen-protected ascorbic acid and salicylic acid on performance of dairy cows under heat stress conditions, forty four lactating dairy cows in early lactation were allocated in a completely randomized design with 4 treatments and 11 animals in each treatment for 30 days. ...
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To study the effects of feeding rumen-protected ascorbic acid and salicylic acid on performance of dairy cows under heat stress conditions, forty four lactating dairy cows in early lactation were allocated in a completely randomized design with 4 treatments and 11 animals in each treatment for 30 days. Experimental treatments were: 1) control (diet without coated ascorbic acid and salicylic acid), 2) diet contained 30 g/d coated ascorbic acid, 3) diet contained 10 g/d coated salicylic acid and 4) diet contained 30 g/d coated ascorbic acid + 10 g/d coated salicylic acid. Cows were fed with salicylic acid supplement had a higher daily milk yield (51.47 kg/day) compared to other experimental groups (P<0.05). Feeding of coated ascorbic acid increased milk fat percent, glucose, BHBA, NEFA concentration and decreased somatic cell count compared to other treatments (P<0.05). Total milk's solids, energy and fat corrected milk and milk urea nitrogen were increased in coated ascorbic acid group compared to control (P<0.05). Blood urea concentration in coated ascorbic acid and salicylic acid group were less than control group (P<0.05). The results showed that feeding coated ascorbic acid and salicylic acid could improve performance of Holstein dairy cows in early lactating phase under heat stress conditions.
Mehdi Mirzaei; Ali Sadeghi Sefid Mazaki; Mohammad Khorvash; Gholam Reza Ghorbani; Pirouz Shakeri
Abstract
This study was carried out to assess the effects of alfalfa hay level and replacing corn grain by other grains in starter on daily feed intake, average daily gain (ADG) and feed efficiency (FE) of pre and post-weaning dairy calves during the pre and post-weaning periods. For this purpose, a database ...
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This study was carried out to assess the effects of alfalfa hay level and replacing corn grain by other grains in starter on daily feed intake, average daily gain (ADG) and feed efficiency (FE) of pre and post-weaning dairy calves during the pre and post-weaning periods. For this purpose, a database Meta-analysis used in the present study. Statistical analyses were performed using PROC MIXED in SAS software. Starter intake and ADG were not affected by alfalfa hay feeding level during the pre-weaning period. However, alfalfa hay feeding tended to increase starter intake during the post-weaning and entire periods (P = 0.07 and P = 0.09, respectively). Further, ADG increased during the post-weaning and entire periods with alfalfa hay supplementation. Starter intake and ADG quadratically affected by the level of alfalfa hay supplementation. At pre-weaning period, feed efficiency tended to decrease (P = 0.06) with alfalfa hay supplementation, but was not different with control group during the post-weaning and entire periods. In addition, partial replacement of corn with other grains increased (P < 0.01) starter intake of dairy calves, but feed efficiency decreased (P < 0.01). Based upon the findings of the present research, alfalfa hay supplementation up to 7.5 % of the total diet could improve starter intake and ADG in dairy calves at post-weaning period. Further, it is possible to replace corn with other grains, up to 50 %, without any negative effect on neonatal dairy calves performance.
Rokhsareh Najadini; Gholam Dashab; mehdi vafae valleh; nasrollah moradi
Abstract
Heat stress is one of the most important environmental stressors challenging poultry production worldwide, especially in warm regions such as Sistan and Baluchestan province of Iran. Heat stress increases the production of free radicals in the chicken’s body. Glutathione peroxidase plays important ...
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Heat stress is one of the most important environmental stressors challenging poultry production worldwide, especially in warm regions such as Sistan and Baluchestan province of Iran. Heat stress increases the production of free radicals in the chicken’s body. Glutathione peroxidase plays important roles as cellular antioxidants in heat stress. The aim of this study was to conduct an analysis of the evolutionary and phylogenetic of GPX-1 in Ross 308 and Khazak populations. Boold samples were collected from 10 birds selected randomly from two stocks of Khazak and Ross 308 population (5 Ross 308 and 5 Khazak birds). DNA was extracted from whole blood. PCR amplification of 800 bp of GPX-1 was performed using one pairs of special primers. Then, PCR product sent for DNA sequencing. Sequence alignment of the GPX-1 fragment revealed a total of 9 haplotypes and 13 variable sites. Out of 13 polymorphic sites, 5 were singletons. Dendrogram of phylogenetic showing genetic similarity between the two populations, but probably diversity within populations indicate the possibility to improve genetic changes and increase the resistance to environmental stresses using selection. The results of genetic distance and polymorphic site of GPX-1 in different species approved phylogenetic tree findings. Study of positive- selection process showed that selection and evolution are playing major roles in understanding the biological function of this gene.ش
Ramin Abdoli; Reza Sourati Zanjani; Seyed Hosein Hosseini Moghaddam; Farjad Rafeie
Abstract
Introduction: Considering the importance of comparing the silkworm performances of Iranian and imported commercial hybrids from China production traits, viability index and cocoon dimensions index were investigated in eight Iranian (31×32, 32×31, 103×104, 104×103, 151×154, ...
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Introduction: Considering the importance of comparing the silkworm performances of Iranian and imported commercial hybrids from China production traits, viability index and cocoon dimensions index were investigated in eight Iranian (31×32, 32×31, 103×104, 104×103, 151×154, 154×151, 153×154 and 154×153), and nine imported Chinese commercial hybrids (Suju×Minghu (S×M), Minghu×Suju (M×S), Baiyue×Qiufeng (B×Q), Qiufeng×Baiyue (Q×B), BaiyueB×QiufengA (BB×QA), QiufengA×BaiyueB (QA×BB), 781×7532, Lianggang No2 and Guican No 5) in the present study.
Materials and Methods: After eggs hatched and according to the standard protocols, the studied hybrids were reared under the same conditions at Iran Silk Research Center (ISRC). Statistical analyses were done in three replications of 200 larvae (totally 600 records per each hybrid) based on completely randomized design. For comparing the means, Tukey's statistical test was used at a significance level of P<0.05. The GLM procedure was used in SAS software version 9.4. Data generated in respect of the studied traits were subjected for further analysis by a multiple trait evaluation index using the following formula: evaluation index (E.I.)= (A-B/C×10) + 50, where A is value of a particular breed for particular trait, B is mean value for a particular trait of all the hybrids, C is standard deviation of a particular trait for all the hybrids, 10 is standard unit and 50 is fixed value. Minimum / average Evaluation Index (E.I) value fixed for selection of a hybrid is >50. Based on the evaluation index (E.I) consisted of production traits and viability, best hybrids were identified.
Results and Discussion: The result of the research showed the close competition between Iranian and Chinese silkworm hybrids. In the situation that the performance of Iranian 32×31 and 31×32 hybrids for traits related to weight, shell weight and shell percentage of best cocoons was higher than the other hybrids but based on a composite index that was used in this research, 151×154, 154×151, 153×154 and 154×153 hybrids showed superiority. Based on this index, M×S and S×M as well as BB×QA and QA×BB had a higher average than the other hybrids. This result is based on the average of two-way crosses for the hybrids that both pairs were used in the experiment.
Conclusion: Generally, the results of mean comparisons and evaluation index showed that Iranian hybrids had better performance in all traits related to produce cocoons than the Chinese hybrids. Although, Chinese hybrids were significantly different from the Iranian for pupa viability percentage trait as Lianggang No2 hybrid showed the highest value.
Kamel Amozadeh Araee; Taghi Ghoorchi; Abdolhakim Toghdory
Abstract
Introduction: Minerals affect wool production in sheep by affecting feed intake, altering rumen function and affecting the flow of nutrients from the rumen or by directly disrupting the animal's metabolism. Wool has the highest concentration of sulfur, which indicates the higher need of wool-producing ...
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Introduction: Minerals affect wool production in sheep by affecting feed intake, altering rumen function and affecting the flow of nutrients from the rumen or by directly disrupting the animal's metabolism. Wool has the highest concentration of sulfur, which indicates the higher need of wool-producing sheep for sulfur than other breeds. The purpose of this study was to investigate the effect of different levels of bentonite sulfur on yield, wool quality, blood minerals, liver enzymes and thyroid hormones of ewes.Materials and Methods: An experiment was conducted to evaluate the use of different levels of bentonite sulfur in sheep. For this purpose, 18 Dalagh ewes (average weight 38 ± 3.5 kg) were kept individually for 42 days with three treatments and 6 repetitions. Experimental treatments included: 1) control (without bentonite sulfur), 2) treatment containing 0.15 percent of bentonite sulfur and 3) treatment containing 0.3 percent of bentonite sulfur(based on dry matter). At first, to ensure the health of all the ewes, they are subjected to a thorough and thorough examination. The bentonite sulfur used in this research contanied 90% sulfur and 10% bentonite. The diets used in this experiment were prepared and adjusted according to the tables of NRC and were given to the ewes in the morning and evening. Ewes were weighed at the beginning and the end of the period after 16 hours of starvation using a digital scale. In order to determine the quality of sheep's wool, in the initial test, the ewes were arranged uniformly on the 41st day of the experiment, and samples were taken from the left side of the ewes in a 10 x 10 cm format according to the common and standard instructions using a manual wool picker. For determination of blood parameters, on the last day of the experiment, three hours after morning feeding, blood samples were taken from the jugular vein of the ewes.Results and discussion: The results obtained from this research showed that with the increase in the level of bentonite sulfur in the diet, the length of fibers, efficiency, strength, kemp and the coefficient of variation of fiber diameter increased (P<0.05). Also, increasing the level of bentonite sulfur increased the concentration of serum sulfur and triiodothyronine hormone (P<0.05). Bentonite sulfur supplementation had no significant effect on final weight, daily weight gain, dry matter intake and feed conversion ratio of ewes. Also, different levels of bentonite sulfur had no significant effect on Non-Medullated fibers, Medullated fibers, average diameter and fiber crude protein. copper gallate, calcium, selenium, phosphorus and serum molybdenum. Addition of bentonite sulfur had no significant effect on serum concentrations of copper, calcium, selenium, phosphorus, molybdenum as well as the concentration of liver enzymes of experimental ewes. Conclusion: The results of this study indicated that the use of bentonite sulfur up to the level of 0.3% of dry matter, could improve the strength and efficiency of wool fibers and it can be used in feeding ewes without having negative effects on their health and performance.
Fatemeh Hendijani; Jamal Fayazi; Hedayat-allah Roshanfekr; Mohammad Reza Ghorbani
Abstract
In order to investigate the effect of vitamin E and vitamin E nanoliposomes on the expression of the STAR gene in testis and TSPO gene in the ovary of Japanese quail, an experiment using 864 pieces of quail for 10 weeks in a completely randomized design with six treatments, six replications and ...
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In order to investigate the effect of vitamin E and vitamin E nanoliposomes on the expression of the STAR gene in testis and TSPO gene in the ovary of Japanese quail, an experiment using 864 pieces of quail for 10 weeks in a completely randomized design with six treatments, six replications and 24 Breeding quail (16 females and eight males) was performed in each replication. The treatments were fed diets containing different levels of vitamin E (25, 50 and 100 IU per kg of diet) and vitamin E nanoliposomes (25, 50 and 100 IU per kg of diet). The results of this study showed that the effect of the experimental treatments on the expression of TSPO gene in the ovary and STAR gene in the testis was significant (P<0.05). The addition of 50 IU of vitamin E significantly increased TSPO gene expression in the ovary compared to the control treatment (P<0.05). The results also showed that the level of 25 IU nanoliposome of vitamin E increased the expression of TSPO gene in the ovary, which was not significantly different from the control treatment. The use of vitamin E and vitamin E nanoliposome levels significantly decreased the expression of STAR gene in the testis of Japanese quail (P<0.05). According to the results of this study, the addition of 25 IU vitamin E nanoliposomes as well as 50 and 100 IU levels of vitamin E has a significant effect on the expression of TSPO gene in the ovary, which is one of the genes affecting fertility and reproduction.
Mohadeseh Namvar; Gholam Reza Dashab; Mohammad Rokouei; Hadi Faraji Arogh; Davoud Ali Saghi
Abstract
This study was performed to compare different models for estimation of direct and maternal heritability and also for obtaining of genetic trend for body weight traits in Kurdi sheep of North Khorasan. The records of body weight in birth (BW, 7345 records), 3-month (3W, 5905 records), 6-month (6W ,5294 ...
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This study was performed to compare different models for estimation of direct and maternal heritability and also for obtaining of genetic trend for body weight traits in Kurdi sheep of North Khorasan. The records of body weight in birth (BW, 7345 records), 3-month (3W, 5905 records), 6-month (6W ,5294 records), 9-month (9W, 3800 records) and 12-month (12W, 3863 records) weight that collected during 1990 to 2013 by Sheep Breeding Station of Shirvan were used. Estimation of genetic parameters for different traits by using of six animal models was carried out by ThrGibbsf90 software. The most suitable model for each trait was determined based on Deviance Information Criterion (DIC). The estimates of direct heritability for BW, 3W, 6W, 9W and 12W traits were 0.172± 0.0007, .0.257± 0.0007, 0.351± 0.0006, 0.120± 0.0007 and 0.131± 0.0009, respectively. In this study, the material genetic effect was significant for BW, 3W and 6W traits (P<0.01) and proportion of maternal permanent environmental variance to phenotypic variance was varied from 0.055 (12W) to 0.186 (BW). Although estimated heritability of maternal effects for body weight was lower in older ages, the result of this study showed that including maternal effects (maternal genetic and permanent environmental effect) in the statistical model could lead to more accurate estimation of genetic parameters for growth traits in all ages. The phenotypic trend for BW, 3W, 6W, 9W and 12W were 8.4, 74.6, 8.3, 54.3, 78.3 gr/year, respectively and direct additive genetic trend for mentioned traits were -0.07, 14.2, -21.9, 13.7, 24.9 gr/year, respectively. Therefore, the results of bayesian analysis are more reliable than convientioal statistical methods.
alireza aghashahi; hadi hosseini; sayed abdoullah hosseini; AMIRREZA SAFAEI; Ali Reza Yousefi; Jafar Ramazankhany
Abstract
This study was aimed to investigate the effect of adding dried dairy wastes to the starter diet on growth performance in Holstein dairy calves. A total of 28 female newborn Holstein dairy calves were randomly allocated to four groups (n=7/group) and after two weeks of habituation to experimental conditions ...
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This study was aimed to investigate the effect of adding dried dairy wastes to the starter diet on growth performance in Holstein dairy calves. A total of 28 female newborn Holstein dairy calves were randomly allocated to four groups (n=7/group) and after two weeks of habituation to experimental conditions and diet, in a completely randomized design, individually fed starter diets containing 0 (control), 10, 20, or 30% dried dairy waste up to eight weeks of age. Feed intake, weight gain, and feed efficiency (gained weight/consumed feed) were measured weekly, during the experimental period. Results showed that body weight, weight gain, feed intake, and feed efficiency were not affected by the treatments. Interactive effect of treatments by week showed that the highest weight gain was for calves fed by the diet containing 10% dairy waste or control group at first week. However, the highest gain was recorded for those that received 30% dairy waste at week five of the experiment (P<0.05). In conclusion, the results of the present study revealed that the inclusion of dried dairy products up to 30% of calves’ starter diet had no adverse effect on performance, and therefore can be used as an affordable and valuable nutritional by-product of the dairy industry in the nutrition of calves.
Ali Nargeskhani; Ali Moharrery; Hossein Mehraban; Mohammad Javad Zamiri
Abstract
This study was conducted to compare the effect of a cellulosic (wheat straw; WS) and non-cellulosic (beet pulp; BP) forage source on high producing dairy cows during the transition period (three weeks before to six weeks after calving). Multiparous Holstein cows (n=36) with mean body weight of 697±59.6 ...
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This study was conducted to compare the effect of a cellulosic (wheat straw; WS) and non-cellulosic (beet pulp; BP) forage source on high producing dairy cows during the transition period (three weeks before to six weeks after calving). Multiparous Holstein cows (n=36) with mean body weight of 697±59.6 kg and body condition score of 3.3±0.22 were used in the experiment. Before calving, the cows received diets containing 7.17% WS (n=18) or BP (n=18). After calving, the cows in each group were divided into two subgroups (n=9), and received the diets containing 5% BP or WS. Prepartum dry matter intake (DMI) was similar between dietary treatments but prepartum digestibility of DM (P=0.05) and neutral detergent fiber (P= 0.06) was lower in cows feeding on the WS diet. The yield of energy-corrected milk, 4% fat-corrected milk and milk fat percentage tended to be higher in the cows fed with the BP diet during the prepartum period (P = 0.09). In conclusion, the results showed that feeding non-cellulosic forages during the transition period may improve the performance in dairy cows.
Mehrdad Shahizad; hamid Amanlou; Najme Eslamian Farsuni; Tahere Amirabadi Farahani; Hadi Khabazan
Abstract
The objective of present study was to investigate the effect of different levels of sesame meal in early lactation cow's ration on dry matter intake, milkyield and composition, apparent digestibility of nutrients and blood metabolites. Six dairy lactating Holstein cows with average DIM 26± 3 (n=3primiparous ...
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The objective of present study was to investigate the effect of different levels of sesame meal in early lactation cow's ration on dry matter intake, milkyield and composition, apparent digestibility of nutrients and blood metabolites. Six dairy lactating Holstein cows with average DIM 26± 3 (n=3primiparous and n=3 multiparous cows) were randomly assigned to 1 of 3 experimental treatments (different levels of sesame meal) in repeated 3×3Latin square design. Experimental treatments were control (without sesame meal), 4.33% sesame meal and 8.66% sesame meal (DM basis). Theexperimental periods consisted of 21 d with the first 14 days as adaptation and the last 7 days as sampling days. Increasing level of sesame meal inearly lactation rations did not affect dry matter intake, milk yield and composition (fat, protein and lactose), feed utilization efficiency, apparentdigestibility of nutrients and blood metabolites (P 0.05), while milk urea nitrogen decreased with increasing the level of sesame meal (p < 0.05).Blood urea nitrogen concentrations also tended (P = 0.1) to decrease with increasing levels of sesame oil. In general, the lack of response in feedintake and milk yield with increasing the levels of sesame meal in early lactation cow's ration showed that sesame meal could be a proper proteinsource in early lactation cow's ration.
Yousef Naderi
Abstract
The objective of this study was evaluating single-trait and multiple-trait animal models with considering imputation in simulated genomic data to estimate the accuracies of genomic prediction across various genomic scenarios and to detect genotype × environment (G × E) interaction. Genomic ...
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The objective of this study was evaluating single-trait and multiple-trait animal models with considering imputation in simulated genomic data to estimate the accuracies of genomic prediction across various genomic scenarios and to detect genotype × environment (G × E) interaction. Genomic data were simulated to reflect variations in number of QTL (90 and 900) and linkage disequilibrium (LD = low and high) using 50K SNP panel. Afterwards, 90 percent of the markers randomly removed and imputation was performed using FImpute software (version 2.2). The average accuracy of imputation for scenarios with high and low LD was 0.976 and 0.943, respectively. In all scenarios, negligible difference on the genomic accuracies was evident, when original genotypes and imputed genotypes were compared. The genomic accuracy reduced with decreasing the LD, heritability and the genetic correlation among the traits. Comparing to single-trait animal model, using multiple-trait animal model increased genomic accuracy. The level of LD and genetic correlation across environments play important roles providing genotype × environment interaction exists. On the one hand, considering genotype × environment interaction and its effect on increasing of genomic accuracy and imputation of low to high density marker panels (especially high LD scenarios) to reduce of the cost of genomic evaluation on the other hand could be a suitable and practical approach to improve genomic selection.
Abdolhakim Toghdory; Taghi Ghoorchi; Mohammad Asadi; Javad Zareie
Abstract
In order to investigate the effect of feeding milk enriched with garlic powder on performance, digestibility of nutrients, diarrhea status and blood parameters in suckling lambs, twenty-four newborn lambs (birth weight 3.7±0.5) were randomly placed in three treatments and eight replications. The ...
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In order to investigate the effect of feeding milk enriched with garlic powder on performance, digestibility of nutrients, diarrhea status and blood parameters in suckling lambs, twenty-four newborn lambs (birth weight 3.7±0.5) were randomly placed in three treatments and eight replications. The lambs of the control group were fed with milk without garlic powder and the other groups were fed with 1.5 and 3 grams of garlic powder per day, respectively, for 45 days. The results obtained from this research showed that consumption of garlic powder in suckling lambs led to improvement in daily weight gain, final weight and starter feed consumption (P<0.05). Also, treatments caused a significant increase in digestibility of nutrients (P<0.05). The use of garlic powder in experimental lambs improved stool consistency and significantly reduced the number of lambs with diarrhea and the average days of diarrhea (P<0.05). By adding garlic powder, the concentration of glucose and high-density lipoprotein increased (P<0.05). In addition, garlic powder caused a significant decrease in the level of triglyceride, cholesterol and low density lipoprotein in plasma (P<0.05). However, different levels of garlic powder did not make a significant difference on the concentration of urea, albumin, globulin and the ratio of albumin to globulin. According to the present results, it is possible to use garlic powder up to the level of at least 1.5 grams per day as a food additive to improve the performance and health of suckling lambs.