Document Type : Research Paper

Authors

1 Graduated M.Sc., Department of Animal Science, Faculty of Agriculture, Ramin University of Agriculture and Natural Resources (Khouzestan), Mulasany, Iran

2 Assistant Professor, Department of Animal Science, Faculty of Agriculture, Ramin University of Agriculture and Natural Resources Ramin (Khouzestan), Mulasany, Iran

3 Associate Professor, Department of Animal Science, Faculty of Agriculture, Ramin University of Agriculture and Natural Resources (Khouzestan), Mulasany, Iran

Abstract

This study was conducted to detect polymorphism of gene BMP15, a member of Transforming Growth
Factor β (TGFβ) family which has a crucial role in controlling the ovarian follicles development,
ovulation rate and fertility. Samples were randomly selected from 91 Najdi goats in 3 geographical
locations, northwest, southeast and center of Khuzestan province. After DNA extraction, amplification of
235 bp fragment of exon 2 of BMP15 gene was performed using specific primers. Sequence detection
was executed after amplification of gene fragments. The association of BMP15 gene and litter size was
done by SAS software. Results from sequencing were analyzed by Vector NTI software. The results
identified three mutations in bases 529 (T to G), 530 (C to G) and 576 (T to C). The largest litter size
belongs to AA pattern. Point mutations in this gene will alter the Ovulation rate of the goat. Therefore,
improving twining trait in Najdi population can be expected by marker assisted selection.

Keywords

1 . سلیمانی، ب.، رحیمی میانجی، ق. و برومند چ آ (1390) بررسی اثر پلی­مورفیسم در اگزون 2 ژن BMP15 روی دوقلوزایی و صفات وزنی در گوسفند سنجابی. زیست­شناسی ایران. 24(4): 493-487.
2 . علی نقی­زاده، ر.، محمدآبادی، م. ر. و زکی­زاده س (1389) چندشکلی اگزون 2 ژن BMP15 در بز سرخ جبال بارز. مجموعه بیوتکنولوژی کشاورزی. 2(1): 80-69.
3 . محمدی ر (1388) بیوشیمی. ویرایش اول. انتشارات آییژ. تهران، صص. 53-54، 57-59 و 66-67.
 
4 . Arefnezhad B (2007) Molecular analysis of BMP-15 (Bone Morphogenetic Protein-15) gene in Markhoz goats. M.Sc. Thesis, Department of Animal Science, Shiraz University, Shiraz, Iran p. 133.
 5 . Bodensteiner KJ, Clay CM, Moeller CL and Sawyer HR (1999) Molecular cloning of the ovineGrowth/Differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries. Biology of Reproduction 60: 381-386.
6 . Chang HC, Brown W and Matzuk MM (2002) Genetic analysis of the mammalian transforming growth factor-b superfamily. Endocrine Reviews. 23: 787-823.
7 . Dai R (2004) Analyzed relationship between CLPG, BMP15 gene polymorphic and production performance of seven sheep populations in north of Xinjiang. Nanjing Agricul University Master’s Thesis.
8 . Davis GH, Balakrishnan L, Ross IK, Wilson T, Galloway SM, Lumsden BM, Hanrahan JP, Mullen M, Mao XZ, Wang GL, Zhao ZS, Zeng YQ, Robinson JJ, Mavrogenis AP, Papachristoforou C, Peter C, Baumung R, Cardyn P, Boujenane I, Cockett NE, Eythorsdottir E, Arranz JJ and Notter DR (2006) Investigation of the Booroola (FecB) and Inverdale (FecX(I)) mutations in 21 prolific breeds and strains of sheep sampled in 13 countries. Animal Reproduction Science. 92: 87-96.
9 . Galloway SM, McNatty KP, Cambridge LM, Laitinen MP, Juengel JL, Jokiranta TS, McLaren RJ, Luiro K, Dodds KG, Montgomery GW, Beattie AE, Davis GH and Ritvos O (2000) Mutations in an oocytederived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner. Nature Genetics. 25: 279-283.
10 . Hanrahan JP, Gregan SM, Mulsant P, Mullen M, Davis GH, Powell R and Galloway SM (2004) Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biology of Reproduction. 70: 900-909.
11 . He YQ, Chu MX, Wang JY, Fang L and Ye SC (2006) Polymorphism on BMP15 as a candidate gene for prolificacy in six goat breeds. Journal of Anhui Agricultural University. 33: 61-64.
12 . Hua GH, Chen SL, Ai JT and Yang LG (2008) None of polymorphism of ovine fecundity major genes FecB and FecX was tested in goat. Animal Reproduction Science. doi:10.1016/j.anireprosci.3-4: 279-286.
13 . Juengel JL, Hudson NL, Heath DA, Smith P, Reader K, Lawrence SB, connell O, Laitinen ARM, Cranfield M, Groome NP, Ritvos O and McNatty KP (2002) Growth differentiation factor 9 and bone morphogenetic protein 15 are essential for ovarian follicular development in sheep. Biology of Reproduction. 67: 1777-1789.
14 . Knight PG and Glister C (2003) Local roles of TGF-beta superfamily members in the control of ovarian follicle development. Animal Reproduction Science. 78: 165-183.
15 . Nejati-Javaremi A, Rahimi G and Amiri A (2007) Detection of polymorphism in FecXL gene associated with Twinning in Lori-Bakhtiari sheep breed using PCR-SSCP. The 5th NationalBiotechnology Congress of Iran. Tehran, Iran. p. 520.
16 . Wang Y, Yuanxiao L, Nana Z, Zhanbin W and Junyan B (2011) Polymorphism of Exon 2 of BMP15 Gene and Its Relationship with Litter Size of Two Chinese Goats. Asian-Australasian Journal of Animal Sciences. 24(7): 905-911.
17 . Zare Y, Nejati-Javaremi A and Rahimi G (2007) Detection of polymorphisms in two point of gene associated with Twinning (BMP15) in Shal sheep. The 5th National Biotechnology Congress of Iran. Tehran, Iran. p. 483.