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Polymorphism of exon 2 of bone morphogenetic protein 15 gene (BMP15) and its relationship with litter size in Najdi goat

Document Type : Research Paper

Authors

1 Graduated M.Sc., Department of Animal Science, Faculty of Agriculture, Ramin University of Agriculture and Natural Resources (Khouzestan), Mulasany, Iran

2 Assistant Professor, Department of Animal Science, Faculty of Agriculture, Ramin University of Agriculture and Natural Resources Ramin (Khouzestan), Mulasany, Iran

3 Associate Professor, Department of Animal Science, Faculty of Agriculture, Ramin University of Agriculture and Natural Resources (Khouzestan), Mulasany, Iran

Abstract

This study was conducted to detect polymorphism of gene BMP15, a member of Transforming Growth
Factor β (TGFβ) family which has a crucial role in controlling the ovarian follicles development,
ovulation rate and fertility. Samples were randomly selected from 91 Najdi goats in 3 geographical
locations, northwest, southeast and center of Khuzestan province. After DNA extraction, amplification of
235 bp fragment of exon 2 of BMP15 gene was performed using specific primers. Sequence detection
was executed after amplification of gene fragments. The association of BMP15 gene and litter size was
done by SAS software. Results from sequencing were analyzed by Vector NTI software. The results
identified three mutations in bases 529 (T to G), 530 (C to G) and 576 (T to C). The largest litter size
belongs to AA pattern. Point mutations in this gene will alter the Ovulation rate of the goat. Therefore,
improving twining trait in Najdi population can be expected by marker assisted selection.

Keywords

References
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Animal Production
Volume 15, Issue 2 - Serial Number 2
October 2013
Pages 101-107
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