Asma Absalan; Golnaz Taasoli; Shahryar Kargar; Farshid Fatahnia; Zohre Kowsar; Alidad Boostani
Volume 21, Issue 1 , April 2019, , Pages 11-22
Abstract
This experiment was aimed to study the effect of multi enzyme and bacterial inoculant on chemical composition and fermentation parameters of barley silage. Robovio multi enzyme was added at three levels, 0, 500 and 1000 mg/kg of barley dry matter (DM) and Prosage bacterial inoculant (a mixture of Lactobacillus ...
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This experiment was aimed to study the effect of multi enzyme and bacterial inoculant on chemical composition and fermentation parameters of barley silage. Robovio multi enzyme was added at three levels, 0, 500 and 1000 mg/kg of barley dry matter (DM) and Prosage bacterial inoculant (a mixture of Lactobacillus buchneri,Lactobacillus plantarum, Enterococcus faecium and Propionibacterium acidophilus) was added at three levels, 0, 5 and 10 mg/kg of silage DM. Chemical composition, in vitro gas production, protozoa population and protein fractions of experimental silages were measured. Results showed that multi enzyme addition decreased NDF (P<0.05), pH, ash, B2 and C fractions (P<0.01) and increased A and B3 fractions (P<0.01) compared to the control. Barley silage containing 5 mg bacterial inoculant had greater DM, pH and B2 fraction (P<0.01) and lower C fraction compared to the control and silage containing 10 mg of bacterial inoculant. Protozoa population, N-ammonia concentration, estimated metabolizable energy, short chain fatty acids and in vitro organic matter digestibility were not affected (P>0.05). Based on these results use of bacterial and multi enzyme additives for the preparation of wilted barley silage is not recommended due to their costs.
Zohreh Mozduri; Mohammad Reza Bakhtiarizadeh
Volume 18, Issue 1 , April 2016, , Pages 13-26
Abstract
This study was done to gain insights into transcriptional regulation of negative energy balance (NEB) assoctiated genes. Overexpressed genes in NEB were identified using microarray and RNA-seq data and promoter analysis of these overexpressed genes was applied to identify novel transcription factors. ...
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This study was done to gain insights into transcriptional regulation of negative energy balance (NEB) assoctiated genes. Overexpressed genes in NEB were identified using microarray and RNA-seq data and promoter analysis of these overexpressed genes was applied to identify novel transcription factors. Moreever, STRING database was used to construct a regulatory network of identified transcription factors. The results of the gene expression analysis revealed that eight genes in severe NEB are more frequent and significant (P<0.05) in comparison to the mild NEB. Promoter analysis showed that promoters of overexpressed genes are enriched in putative binding sites for 19 transcription factors. This group included known NEB-associated transcription factor (NF-κB), and a number of transcription factors (such as SP1, ZBP89, NFI, Zf9, MYC, ZBTB7A, FOXF2 and KLF6) that had not been previously reported to be associated with NEB. Based on the present results, 18 new effective candidate trsnacription factors introduced in this study can provide new information to gain a better understanding of the regulatory network involved in NEB.
Maryam Bazgiri; Mohammad taghi Beigi nassiri; Jamal Fayazi
Volume 19, Issue 1 , May 2017, , Pages 13-22
Abstract
This study was conducted to investigate the Single nucleotide polymorphism of STAT1 gene by PCR-RFLP technique and association of genotypes with milk production traits in Brown Swiss dairy cattle. The blood samples of ninety-eight cows were collected randomly from tail vein. The DNA was extracted with ...
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This study was conducted to investigate the Single nucleotide polymorphism of STAT1 gene by PCR-RFLP technique and association of genotypes with milk production traits in Brown Swiss dairy cattle. The blood samples of ninety-eight cows were collected randomly from tail vein. The DNA was extracted with Cinagene kit (PR881612C). Fragment of 314 bp in 3'UTR of STAT1 gene was amplified by PCR and digested by Pag1 restriction enzyme. Genotype frequencies were calculated by PopGene software (version 1.31). The SAS 9.1 software was used for statistical analysis and the mean of traits was compared by Duncan test. The genotypefrequencies of TT, TC and CC were 0.23, 0.25 and 0.52, respectively. The T and C allele frequency were 0.355 and 0.645, respectively. The expected heterozygosity was estimated 0.458. Result of Chi-square showed that this locus was not in the Hardy Weinberg equilibrium (P<0.05). The means of traits for milk, fat percent and fat yield, and protein yield were not significant but means of protein percent was significant (P<0.05). The mean of milk, fat and protein yield traits for CC genotype were more than two other genotypes. The results of present study showed that T allele is known as minor frequency allele and C allele have positive effect on milk, fat and protein yield traits.
hamide nuri sadegh; ali maghsoudi; mohammad rokoei; Hadi Faraji Arogh; mehdi jahantigh
Volume 24, Issue 1 , April 2022, , Pages 13-21
Abstract
The aim of this research was to investigate the immune system responses against sheep red blood cells (SRBC) and Newcastle disease virus (NDV) in two sexes of both wild and Italian speckled quails. To compare phenotypic differences of two sexes for humoral immune responses a one-way analysis was applied, ...
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The aim of this research was to investigate the immune system responses against sheep red blood cells (SRBC) and Newcastle disease virus (NDV) in two sexes of both wild and Italian speckled quails. To compare phenotypic differences of two sexes for humoral immune responses a one-way analysis was applied, and a simple animal model using the MCMCglmm package of R software was used for genetic analysis, assuming variance components between the two sexes are different. Phenotypic comparison between males and females did not show a significant difference in any of the strains. In both strains, heritability of humoral immunity in males was higher than females. Results indicated that heritability of IgT in males (0.187), and IgY in females (0.177) were higher than other estimates in wild strain. The highest heritability was related to the NDV, which was estimated to be 0.214 and 0.268 in males and females, respectively. Therefore, genetic selection for IgN can be expected to improve the performance of the birds’ humoral immune system. Likewise, according to the higher estimates of immune responses in males, genetic selection of humoral immune responses for IgN, leads to higher genetic progression.
HamidReza Dadkhah; GholmReza Ghorbani; Farzad Hashemzadeh; Abbas Rajaeerad
Volume 25, Issue 1 , April 2023, , Pages 13-24
Abstract
The objective of this study was to evaluate the effect of feeding two oral Ca boluses at calving and 12 h later on feed intake, milk yield, body weight (BW) changes, blood calcium and some reproductive parameters of high producing dairy cows during summer season. Twenty-four Holstein cows with average ...
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The objective of this study was to evaluate the effect of feeding two oral Ca boluses at calving and 12 h later on feed intake, milk yield, body weight (BW) changes, blood calcium and some reproductive parameters of high producing dairy cows during summer season. Twenty-four Holstein cows with average parity of 2.8 ± 0.8 were selected and randomly assigned to one of two treatments including control or the group receiving slow-release oral Ca bolus at calving and 12 h post-calving. Row milk yield was greater in cows receiving Ca boluses compared to control treatments during 21 days of lactation (P = 0.02). The milk fat (P = 0.01) and solid non-fat percentage (P = 0.05) were greater in cows that received calcium bolus compared to control treatment. In addition, energy-corrected and fat-corrected milk yields increased in cows received Ca bolus (P = 0.03). Plasma glucose and serum calcium concentration as well as BW change were not affected by experimental treatments. The pregnancy per artificial insemination, days to first insemination and open days were not significantly different between the experimental treatments (P >0.05). The results showed that using oral calcium bolus at calving and 12 hours after calving improved milk production and composition, but had no effect on serum Ca, BW change during first 21 days of lactation, and reproduction parameters.
Mahdieh Taheri; Reza Tahmasbi; Mohammad mehdi Sharifi Hosseini; Omid Dayani
Volume 20, Issue 1 , May 2018, , Pages 15-27
Abstract
The effect of feeding ensiled licorice pulp with different levels of wasted date was investigated on ruminal fermentation parameters, blood parameters and microbial protein synthesis on four male mature Raeini goats. The experiment was designed as a 4×4 Latin square design with four 21 days periods. ...
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The effect of feeding ensiled licorice pulp with different levels of wasted date was investigated on ruminal fermentation parameters, blood parameters and microbial protein synthesis on four male mature Raeini goats. The experiment was designed as a 4×4 Latin square design with four 21 days periods. Licorice pulp was mixed with 0, 5, 10 and 15 percent of wasted date without stone and ensiled in 40 L containers for 45 days. Experimental diets were: 1) control (20% ensiled Licorice pulp without wasted date, 2) 20% ensiled licorice pulp with 5% wasted date, 3) 20% ensiled licorice pulp with 10% wasted date and 4) 20% ensiled licorice pulp with 15% wasted date. The results showed that ensiling licorice pulp with different levels of wasted date significantly increased DM and fleig point linearly (p
Wahidullah Charkhi; Fardin Hozhabri; Hadi Hajarian
Volume 26, Issue 1 , March 2024, , Pages 15-31
Abstract
Introduction: Medicinal plants are one of the most important known herbal additives in feeding ruminant animals. It has been reported that medicinal plants with favorable effects on rumen fermentation improve the function of the digestive system and as a result feed intake, as well as the level of animal ...
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Introduction: Medicinal plants are one of the most important known herbal additives in feeding ruminant animals. It has been reported that medicinal plants with favorable effects on rumen fermentation improve the function of the digestive system and as a result feed intake, as well as the level of animal products and the growth rate of infants increases. Also, numerous and sometimes contradictory reports have been reported about the effects of using medicinal plants in the diet of different animals on blood parameters. Limited studies have been published regarding the addition of medicinal plants to the diet of mother ewes and its effect on the suckling lambs. Most of the studies are about the direct use of medicinal plants by lambs and its effect on the performance and other behavioral characteristics of the animal. Therefore, in the present study, the main goal was to investigate the effects of adding a mixture of different proportions of medicinal plants such as cumin, coriander seeds and peppermint to the ration of lactating ewes on the performance, blood parameters, and antioxidant status of the suckling lambs.
Materials and Methods: This research was conducted in a completely randomized design using 48 lambs with an average weight of 3.34±0.44 kg along with 24 lactating ewes with an average milk production of 1107.03±122.25 grams per day in individual stalls for a period of 65 days. The groups of mother ewes included: control (basic diet) and first, second and third treatments, in addition to the basic diet, were received 15 grams of cumin, coriander and peppermint mixed powder with the ratios of 60:30:10; 10:45:45 and 30:60:10 %, respectively. The lambs were weighed every two weeks and at the end of the experiment, blood was taken from the jugular vein.
Results and Discussion: The final weight and average daily gain (ADG) of lambs of first treatment were higher than other groups (P<0.05). Red blood cells (RBC) and hematocrit were the lowest in third and the highest in first treatments, although there was no significant difference with control. Platelets and red cell distribution width (RDW) in first treatment were lower than control (P<0.05). There was no difference between treatments in terms of white blood cells (WBC), percentages of lymphocytes and granulocytes. The concentration of blood glucose in lambs of first treatment decreased compared to control. The total antioxidant capacity of lambs from mothers receiving herbal supplement increased and malondialdehyde was lower compared to control.
Conclusion: The results showed that the use of a mixture of cumin, coriander and peppermint in the ration of mother ewes increased the antioxidant capacity and decreased malondialdehyde in suckling lambs, although ADG was not noticeable.
Ali Kalantari-Hesari; Mohammad reza Asadi; Mohammad Babaei
Volume 24, Issue 1 , April 2022, , Pages 47-58
Abstract
The effect of Betaplus probiotic levels in the diet on growth performance and histomorphometry of Japanese quail jejunum was investigated by using 288 broiler chicks with six treatments of four replications and 12 birds per each in a completely randomized design from one to 42 days of age. Treatments ...
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The effect of Betaplus probiotic levels in the diet on growth performance and histomorphometry of Japanese quail jejunum was investigated by using 288 broiler chicks with six treatments of four replications and 12 birds per each in a completely randomized design from one to 42 days of age. Treatments included: control group (without additives); three diets containing levels of 0.5, 1 and 1.5 g/kg of Betaplus probiotic; diet with deficient in protein, and diet with deficient in protein and contained 1 g/kg of Betaplus. Feed intake, body weight and increase body weight were measured weekly and conversion factor was calculated. At 42 days of age, two birds were randomly selected from each replicate and the traits related to carcass dissection were assessed. For histomorphometrical evaluation of the intestine, the jejunum samples were collected. The utility of betaplus probiotic at level 1 g/kg improved food intake, weight gain, conversion factor and some histomorphometrical parameters of jejunum (p <0.05), In birds were feed with level of 1.5 g/kg Betaplus, the villi thickness and crypt diameter and number of the goblet cells were decreased (p <0.05). According to the results, the use of Betaplus probiotic at level 1 g/kg in the diet improves the growth performance and histological parameters of the jejunum in Japanese quail.
Bahriye Shahi; Rasoul Pirmoohamadi; Abdolrahman Amini
Volume 16, Issue 2 , October 2015, , Pages 85-92
Abstract
The aim of this study was to evaluate the effects of different levels of Citrus Limon essential oil on nutrient digestibility, blood and ruminal metabolites in male Holstein calves. This study were conducted using four male Holstein calves, each fitted with a permanent rumen cannula (average body weight ...
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The aim of this study was to evaluate the effects of different levels of Citrus Limon essential oil on nutrient digestibility, blood and ruminal metabolites in male Holstein calves. This study were conducted using four male Holstein calves, each fitted with a permanent rumen cannula (average body weight 650±20 kg) that received experimental diets including control with 600, 800 and 1000 mg/d lemon essential oil and control with monensin 300 mg/d in a change-over complete randomized block design with five periods of 17 days. Apparent digestibility of OM in ration including of 1000 mg per day of Citrus Limon was highther than the others treatments (P<0.05). Experimental treatments had not affected on DM, CP, EE, NDF and ADF digestibility. The effect of treatments were not significant on glucose, HDL, albumin, total protein, cratinin, and triglyceride and urea concentration, but cholesterol values and BHB concentration in serum of control treatment were more than the other treatments (P<0.05). The concentration of propionate in rumen of animals received 1000 mg Citrus Limon essential oil, was more than control of treatment, but their concentration of butyrate was low than control treatment (P<0.05). In conclusion, results showed that utilization of 1000 mg per day dose of Citrus Limon essential oil in diet could improve the OM digestibility, rumen fermentation characteristics and blood BHB concentration of male Holstein calves.
Hasan Moradian; Ali Esmailizadeh; Mohammadreza Mohammadabadi
Volume 15, Issue 2 , October 2013, , Pages 89-99
Abstract
The purpose of this study was to identify genomic regions of quantitative trait loci (QTL), affectingcarcass traits on chromosome 1 in an F2 population of Japanese quail. For this purpose, a three-generationresource population was developed by using two distinct Japanese quail strains, wild (meat type) ...
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The purpose of this study was to identify genomic regions of quantitative trait loci (QTL), affectingcarcass traits on chromosome 1 in an F2 population of Japanese quail. For this purpose, a three-generationresource population was developed by using two distinct Japanese quail strains, wild (meat type) andwhite (layer type). Eight pairs of white and wild birds were crossed reciprocally and 34 F1 birds wereproduced. The F1 birds were intercrossed to generate 422 F2 offspring. Phenotypic data including weightof carcass parts were collected on F2 birds. All of the animals from three generations (472 birds) weregenotyped for eight microsatellite markers on chromosome 1. QTL analysis was performed with leastsquares interval mapping method fitting three various statistical models. Significant QTL were identifiedfor breast weight, carcass weight, head weight and percentage of breast. There was also evidence forimprinted QTL affecting breast weight, a carcass part of high economic value, on chromosome 1. Theproportion of the F2 phenotypic variation explained by the significant additive, dominance and imprintedQTL effects ranged from 1.8 to 2.3, 1.2 to 3.3 and 0.5 to 2.2 percent, respectively.
Hossein Mohammadi; Amir Hossein Khaltabadi Farahani; Mohammad Hossein Moradi; mohammad shamsollahi
Volume 25, Issue 2 , July 2023, , Pages 133-143
Abstract
Introduction: The selection of animals by humans left detectable signatures on the genome of modern goat. The identification of these signals can help us to improve the genetic characteristics of economically important traits in goat. Over the last decade, interest in detection of genes or genomic regions ...
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Introduction: The selection of animals by humans left detectable signatures on the genome of modern goat. The identification of these signals can help us to improve the genetic characteristics of economically important traits in goat. Over the last decade, interest in detection of genes or genomic regions that are targeted by selection has been growing. Identifying signatures of selection can provide valuable insights about the genes or genomic regions that are or have been under selection pressure, which in turn leads to a better understanding of genotype-phenotype relationships. A run of homozygosity (ROH) is a consecutive tract of homozygous genotypes in an individual that indicates it has inherited the same ancestral haplotype from both parents. Run of homozygosity one of the most methods were used to detecting the genomic inbreeding. The locations of ROHs which are under positive selection, or laboring favorable allele in population, tend to be fixed in the genome and formation of ROH Island during long times. Genomic regions enriched with ROH may be indicative of selection sweeps and are known as ROH islands. As detecting the ROH Islands, the genomic regions contain economic traits could be detectable.
Materials and Methods: In this research, the amount of genomic inbreeding and the effective size of the population were investigated using the information obtained from 879 goats of different breeds including Beetal, Daira Deen Panah, Nachi, Barbari, Teddi, Pahari, and Pothwari. In order to determine the genotype of the samples, Illumina caprine Bead Chip 50K were used. The genomic information of goat breeds was extracted from the figshare database. Quality control was conducted using the Plink software. The markers or individuals were excluded from the further study based on the following criteria: unknown chromosomal or physical location, call rate <0.95, missing genotype frequency >0.05, minor allele frequency (MAF) < 0.05, and a P-value for Hardy–Weinberg equilibrium test less than 10-3. After quality control, 36,861 SNPs from Goat SNP chip 50K on 827 goats were remained for the future analysis. Inbreeding coefficient was calculated using four methods including, genomic relationship matrix (FGRM), excess of homozygosity (FHOM), correlation between uniting gametes (FUNI) using the GCTA 1.0 software and run of homozygosity (FROH) using the PLINK 1.9 software. The effective population size (Ne) was calculated from linkage disequilibrium data with SNeP software (version 1.1). GeneCards (http://www.genecards.org) and UniProtKB (http://www.uniprot.org) databases were also used to interpret the function of the obtained genes.
Results and Discussion: The lowest and highest inbreeding coefficient calculated by three methods (FGRM, FHOM, and FUNI) were related to Beetal and Barbari breed, respectively. The highest (0.159) and lowest (0.028) amount of FROH was estimated in the Barbari and Pothwari breeds, respectively. The average length of ROH ranged from 70.2 to 391.4 Mb, and the average number of ROH fragments varied between 8.19 and 48.65. Also, the highest and lowest number of ROH were observed on chromosome 2 and 29, respectively. The size of Ne in in the current generations (fifth generation) of the studied breeds was ranged from 35 to 365. The highest Ne was estimated in the Beetal breed (365 heads) and the lowest in the Barbari breed (35 heads). The average inbreeding coefficient in Beetal, Teddi, Pahari, Nachi, Barbari, Daira Deen Panah and Pothwari breeds was obtained 0.035, 0.081, 0.031, 0.052, 0.15, 0.11 and 0.02, respectively. In addition, the Ne of most of the studied populations has been decreased. The results of this study revealed that, the selection processes in different goat breeds for economic traits during several years, has led to the formation of many ROH islands in goat genome, therefore scanning these regions at the genome level can be an alternative strategy to identify genes and associated loci with economic traits.
Conclusions: our findings contribute to the understanding of genetic diversity and population demography, and help design and implement breeding and conservation strategies for study goat breeds. Therefore, it is necessary to economize production and planning a suitable mating scheme to control inbreeding and genetically conserve the remaining pure animals of these breeds.
Safora Yousefinejad; Farshid Fatahnia; Mehdi Kazemi Bonchenari
Volume 23, Issue 2 , July 2021, , Pages 165-177
Abstract
The aim of this experiment was to investigate the effect of starter soybean oil (SO) and crude protein (CP) level on growth performance, plasma parameters and urinary purine derivatives (UPD) of dairy calves. Forty-eighth 3-old Holstein dairy calves with initial BW of 39.8 ± 2.4 kg were used in ...
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The aim of this experiment was to investigate the effect of starter soybean oil (SO) and crude protein (CP) level on growth performance, plasma parameters and urinary purine derivatives (UPD) of dairy calves. Forty-eighth 3-old Holstein dairy calves with initial BW of 39.8 ± 2.4 kg were used in a completely randomized design with 2 × 2 factorial arrangement. The experimental diets consisted of diet without SO and 19% CP, diet without SO and 22% CP, diet with 3% SO and 19% CP and diet with 3% SO and 22% CP. Feed intake, feces score, skeletal growth indices, plasma parameters, liver enzymes, urinary purine derivatives and microbial protein synthesis were measured. Growth performance, plasma parameters and UPD were not influenced by interaction effect of starter diet SO and CP level. Starter diet intake, average daily gain and feed efficiency were lower in calves fed SO than those fed diets without SO (P < 0.05). The fecal score and wither height of calves fed diets containing SO were higher and lower that those fed diets without SO, respectively (P < 0.05). Plasma concentrations of glucose, beta-hydroxy butyrate and total protein were lower in calves fed SO (P < 0.05). Addition of SO to the diets decreased urinary allantoin and total UPD concentrations (P ≤ 0.05). Microbial protein synthesis tended to be decreased in calves received diets containing SO compared to other groups (P=0.06). Plasma urea nitrogen concentration of calves increased by increasing dietary CP level (P < 0.05). According to this results, growth performance of Holstein dairy calves is not affected by interaction of SO and CP level in starter diet
Fatemeh Jafari; Mohammad Taghi Beige Nasiri; Jamal Fayazi; Mohammad Ali Loghavi
Volume 17, Issue 2 , October 2015, , Pages 171-182
Abstract
Genetic parameters direct and maternal in Fars native flows using the Bayesian statistical approach, was estimation with consider body weight at a day of age (BW1), body weight at eight weeks of age (BW8), body weight at 12 weeks of age (BW12), age at sexual maturity (ASM), egg number (EGP) and mean ...
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Genetic parameters direct and maternal in Fars native flows using the Bayesian statistical approach, was estimation with consider body weight at a day of age (BW1), body weight at eight weeks of age (BW8), body weight at 12 weeks of age (BW12), age at sexual maturity (ASM), egg number (EGP) and mean egg weight during 28th, 30th and 32nd week ages (AV283032) Fars native flows. Genetic parameters were estimated by six different animal models and MTGSAM software. The best model was determined using the Akaike information criterion for each trait. The direct heritability (h2) estimates basis best model BW1, BW8, BW12, ASM, EGP and AV283032 0.36, 0.33, 0.30, 0.48, 0.26 and 0.25, respectively. Maternal effects were significant for ASM, EGP and AV283032 traits studied and estimates of maternal environmental variance as a proportion of phenotypic variance (c2) varied from 0.01 (EGP and AV283032) to 0.39 (BW1). Results present research showed that Bayesian approach by considering all factors more complicated models was selected as a model.
Ali Vojdan Hassan Kiyadeh; Mohammad Rokouei; Gholam Reza Dashab; Ahmad Reza Seyedalian; Hadi Faraji- Arough
Volume 21, Issue 2 , July 2019, , Pages 181-192
Abstract
This research was conducted with the aim of estimation of environmental factors affecting survival and estimate the genetic parameters survival from 1 to the last recording date in Zandi sheep. The survival records of 9558 Zandi sheep from 273 rams and 2328 ewes collected by Animal Breeding Center of ...
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This research was conducted with the aim of estimation of environmental factors affecting survival and estimate the genetic parameters survival from 1 to the last recording date in Zandi sheep. The survival records of 9558 Zandi sheep from 273 rams and 2328 ewes collected by Animal Breeding Center of Iran during the 25 years (1987 to 2011) were used in the analysis. The effective factors on survival and culling risk were estimated by Survival and cmprsk package. Estimation of variance components was performed by using of exponential distribution for censored dates by the fitting of model with additive genetic effect (model 1) and joint additive genetic and maternal permanent environmental of effects (model 2) under Bayesian approach and Gibbs sampling. A total of 1000000 samples with a burn-in of 100000 and a sampling interval of 75 were generated to estimate the posterior distribution of variance components. The results showed that year, month, birth weight, type birth, sex (P<0.001) and dam age (p<0.01) had significant effect on survival trait. the direct heritability of survival trait using models 1 and 2 was 0.184( 0.136-0.264) and 0.162( 0.120-0.202), respectively. the proportion of permanent variance to phenotypic variance was 0.046( 0.063- 0.031). According to the results of this study, genetic improvement of survival trait in zandi lamb is possible by genetic selection and the culling risk reduction is effective via management of environmental factors.
Parvin Shariati Gazgazareh; ALI AKBAR MASOUDI; Rasoul Vaez Torshizi; Alireza Ehsani; Zaynab Mousavian
Volume 22, Issue 2 , June 2020, , Pages 199-209
Abstract
The aim of this study was the investigation of gene expression profile in Shal sheep ovarian tissue using RNA sequencing data. For this purpose, the ovaries of five Shal sheep were isolated after estrous synchronization and their RNA was sequenced using Illumina Hiseq 4000 technology. On average, ...
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The aim of this study was the investigation of gene expression profile in Shal sheep ovarian tissue using RNA sequencing data. For this purpose, the ovaries of five Shal sheep were isolated after estrous synchronization and their RNA was sequenced using Illumina Hiseq 4000 technology. On average, the data obtained from the sequencing consisted of 26638311 read pairs with 81.08 unique mapping rate. The results of bioinformatic analyzes revealed the expression of 21085 genes in Shal sheep ovarian tissue, of which 15078 genes had expression mean above 10. Gene ontology analysis revealed the significant enrichment of 162 GO terms including 41 biological processes, 46 molecular functions and 75 cellular components. KEGG pathway analysis also identified 149 significant pathways (P <0.05), most important of which were estrogen signaling pathway, TGF-beta signaling pathway and oocyte meiosis. Investigating the expression of major genes for twining and reproduction, showed a high expression for INHA, INHBA and BMPR1B, so that INHA, an important paracrine factor in ovarian follicles, was one of the 10 genes with the highest expression. Also, FSHR, ESR1 and ESR2 showed medium expression and GDF9, BMP15 and PRLR showed low expression in the samples. For the first time, in this study the ovarian tissue transcriptome of Shal ewes was comprehensively studied using RNA-Seq technology and this study can provide a useful genetic basis for a better understanding of the genes and processes involved in the Shal sheep reproduction.
Somayeh Zamani afshar; Taher harkinezhad; abas Bahari; mohamad hossein shahir
Volume 20, Issue 2 , August 2018, , Pages 213-224
Abstract
Selection of live animals with minimum carcass fat in animal breeding programs will lead to increase in meat production at national level. The ligands of Wnt genes are of the effective factors in adipocyte cell differentiation. This study was aimed to assess the association between polymorphisms in Wnt10a ...
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Selection of live animals with minimum carcass fat in animal breeding programs will lead to increase in meat production at national level. The ligands of Wnt genes are of the effective factors in adipocyte cell differentiation. This study was aimed to assess the association between polymorphisms in Wnt10a and Wnt10b genes and carcass traits in 96 Afshari – Booroola Merino male lambs. In this study, DNA was extracted from blood samples using phenol-chloroform extraction method and polymerase chain reaction was performed for amplification a 663 bp fragment of exon III and a part of the second intron of Wnt10a gene and a 512 bp fragment of exon III Wnt10b gene. The results showed a polymorphism and three monomorphisms (a different allele compared to reference sequence of the gene) in the Wnt10a gene but all the sequences of the Wnt10b fragment were the same in studied region of the gene. Results of the sequencing lead to identification of four single nucleotide changes in Wnt10a gene in the studied area when compared to the reference sequence. One of the monomorphisms was in the intron and among other three nucleotide changes identified in exon III one was a missense in codon 139. After digestion with restriction enzymes HpaII at this codon, it was observed that all sampled lambs had mutant homozygous genotype compared to the reference sequence. This site was also evaluated in a number of Afshari (fat- tailed) and Zell (with the least fat tail) but again the same results were observed. Therefore, it seems that in this position the G is the wild type allele in the studied population.
Siamak Mashayekhi; Davoud Hasanloo; Majid Afshar; Mehrdad Nafisi
Volume 18, Issue 2 , June 2016, , Pages 215-223
Abstract
This study carried out for economic investigation on laying hen farms in Tehran province. The data was collected through completion of questionnaire by direct interview from 41 layer farms, which were selected by stratified random sampling method in 2013. Among all types of production functions, the ...
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This study carried out for economic investigation on laying hen farms in Tehran province. The data was collected through completion of questionnaire by direct interview from 41 layer farms, which were selected by stratified random sampling method in 2013. Among all types of production functions, the Cobb-Douglas production function was selected and its coefficients was estimated and average product, marginal product and the ratio of value of marginal product to input prices and inputs’ production elsaticity was estimated. The results showed that more than 87 percent of cost of egg production belongs to the feed and pullet costs. The production elasticity of all studied inputs including feed intake, pullet and labor were positive and less than one, which shows that the producers were utilized these inputs economically. The average production of feed and pullet inputs was 0.49 and 18.5, respectively and on this basis, on an average, 0.49 kilograms eggs were produced for one kilogram of feed and 18.5 kilograms of eggs for one pullet in studied laying hen farms. According to the results, feed intake and pullet were most important effective factors on egg production in Tehran province. Meanwhile, considering the price of purchased inputs and sold egg, producers utilize these inputs economically and efficiently based on the ratio of value of marginal product to input price index.
Zahra Shirnegar; Fardin Hozhabri; Mohammad Ebrahim Nooriyan Soroor
Volume 25, Issue 3 , October 2023, , Pages 255-266
Abstract
Introduction: Considering that soybean is mainly used in human nutrition and its meal is mostly used in poultry feed, and also due to the high price of soybean meal and its impact on the cost of each kilogram of feed, using of other protein sources such as camellia meal in the diet of fattening lambs ...
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Introduction: Considering that soybean is mainly used in human nutrition and its meal is mostly used in poultry feed, and also due to the high price of soybean meal and its impact on the cost of each kilogram of feed, using of other protein sources such as camellia meal in the diet of fattening lambs can reduce the need of soybean meal for ruminant animals. Previous studies have shown that camelina meal with a suitable percentage of omega-3, crude protein, crude fat and poly unsaturated fatty acids (alpha-linolenic acid) makes it a suitable feedstuff for livestock, poultry and fish. However, due to the presence of anti-nutritional compounds such as trypsin inhibitor and glucosinolate, the limitations of camellia meal should be taken into consideration when used in the diet of animals. In many experiments, it has been determined that camelina is a plant resistant to harsh weather conditions compared to other oil plants, has a high yield potential, and it has been reported that it is possible to plant it in crop rotation with cereals. In this study, the possibility of using camelina meal (CM) instead of soybean meal (SBM) in the diet of fattening lambs was investigated.Material and Methods: Twenty-four male lambs aged four to six months with an average weight of 34.21±2.18 kg in a completely randomized design with four treatments and six replications were used. Animals were placed in the individual cages. Treatments included: 1) control (basal diet containing SBM), 2) basal diet with 33% replacement of SBM with CM, 3) basal diet with 67% replacement of SBM with CM, and 4) basal diet with 100% replacement of SBM with CM. Ruminal fluid was taken by esophageal tube and blood samples were taken from the jugular vein on days 0, 45 and the end of the experiment.Results and Discussion: The final weight and daily weight gain were not affected by the replacement of oil meals, but it caused a decrease in dry matter intake (P˂0.05). Ruminal ammonia nitrogen concentration increased in a way that was significant in 33 and 67% replacement (P<0.05). Volatile fatty acids concentration and pH were not affected by treatments. The total population of protozoa and genus Entodinium decreased with the increase in replacement percentage, and according to the interaction effect of time and treatment, the population increased with the increase in the duration of the experiment (P<0.05). The concentration of blood urea increased with the replacement of meals (P<0.05), although this process was influenced by the interaction of treatment and time. With the complete replacement of meals, the concentrations of blood cholesterol and triglycerides increased (P<0.05). Malondialdehyde in the blood of lambs was not affected by replacing SBM with CM. Conclusion: According to the results, camelina meal can be used instead of soybean meal at a maximum level of 67% in diet of fattening lambs.
Rasoul Vaez Torshizi
Volume 19, Issue 2 , August 2017, , Pages 265-280
Abstract
The aim of this study was to compare the performance of immune system in Arian, Native, Arian × native crosses, native × Arian crosses and Ross chickens. Through artificial insemination, a total number of 298 birds produced and reared with 100 Ross strain chickens. Thereafter, 15 to 20 percent ...
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The aim of this study was to compare the performance of immune system in Arian, Native, Arian × native crosses, native × Arian crosses and Ross chickens. Through artificial insemination, a total number of 298 birds produced and reared with 100 Ross strain chickens. Thereafter, 15 to 20 percent of chickens were randomly chosen for immune system performance and some of blood parameters comparison. The studied traits were humoral, cell-mediated and innate immunity responses, plasma protein concentrations and relative weight of lymph organs. Data were analyzed using GLM procedure and means were compared using Tukey method. The highest titer of total antibody was in Arian strain and the lowest was in Ross strain. There was no significant difference between sexes and strains for cell-mediated immunity. Responses in crossbred chickens for humoral and cell-mediated immunity were not significantly different from Ross strain. No significant difference was observed for hematocrit percent between crossbred chickens and commercial strains. In crossbred chickens, the percentage of plasma albumin was significantly higher than the other strains (P<0.05). While, the lysozyme activity of crossbred chickens was significantly lower than Arian strain (p<0.05), it did not indicate any difference with Ross strain. The relative weight of all organs in crossbred chickens was lower than native and higher than commercial chickens. The results of current study indicated that the performance of immune system in crossbred chickens was favorable and in some cases better than the other strains. Therefore, it is possible to create resistance birds with suitable growth performance from crosses of native and commercial strains.
Meysam Kanani; Shahryar Kargar; Mohammad Javad Zamiri; Seyed Mehdi Ghoreishi
Volume 21, Issue 3 , September 2019, , Pages 321-328
Abstract
The aim of this study was to investigate the effect of replacing alfalfa hay (AH) with corn silage (CS) in the finely ground starter diet on health status and blood biochemical parameters of male Holstein calves during pre-weaning period. Thirty (3-d old) newborn calves (40.2 ± 1.28 ...
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The aim of this study was to investigate the effect of replacing alfalfa hay (AH) with corn silage (CS) in the finely ground starter diet on health status and blood biochemical parameters of male Holstein calves during pre-weaning period. Thirty (3-d old) newborn calves (40.2 ± 1.28 kg body weight) were assigned randomly to three experimental groups receiving starter diets containing 10% (on dry matter basis) AH (Control), 5% AH and 5% CS, and 10% CS. The starter diets had the same nutrient composition but dry matter content was highest and lowest in Control and 10% CS groups, respectively. Blood concentration of albumin was higher in calves fed 5% CS diet compared with calves fed Control or 10% CS diets (P ≤ 0.05). Compared with calves on other treatments, calves on Control group were more susceptible to develop diarrhea or pneumonia (P ≤ 0.05) and thereby had lower chance to be treated. No difference was observed among treatment groups for frequency and medication days of diarrhea or pneumonia, but duration of diarrhea was higher in calves on Control group compared with other treatments (P ≤ 0.05). Based on obtained results, feeding CS containing diets (5 or 10%) to male Holstein calves during the preweaning period may have a potential to improve calf health and to decrease costs related to drugs and medication
Davoud Ali Saghi; Ali Mobaraki; Mohammad Robati Baluch; Razieh Saghi
Volume 23, Issue 3 , September 2021, , Pages 325-335
Abstract
The aim of this study was to evaluate the effect of crossbreeding on growth performance and carcass composition of purebred and crossbred Kurdi lambs. Thus, two genetic groups including purebred Kurdi lambs (KK) and crossbred Kurdi*Romanov lambs (KR) were used. For analysis of carcass traits, six lambs ...
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The aim of this study was to evaluate the effect of crossbreeding on growth performance and carcass composition of purebred and crossbred Kurdi lambs. Thus, two genetic groups including purebred Kurdi lambs (KK) and crossbred Kurdi*Romanov lambs (KR) were used. For analysis of carcass traits, six lambs from each group were selected and slaughtered. The number of weaned lambs for KK and KR were 95 and 45, respectively. Our results indicated thatKK purebred lambs had the highest (4.5 kg) and KR crossbred lambs had the lowest (4.1 kg) birth weight (P < 0.05). Crossbred Kurdi*Romanov lambs had higher average daily gain than KK purebred lambs (P < 0.05). The means of final weights of purebred lambs were significantly higher than crossbred lambs (54.8 vs. 41.5 kg, respectively). The genetic groups had no significant effect on cold and hot carcass weights. The effect of crossbreeding on fat thickness, fat-tail and leg was significant (P < 0.05). Crossbred lambs performed better than purebred lambs in the pre-weaning period, but purebred Kurdi lambs performed better than crossbred lambs in the fattening period. In general, it can be reported that the crossbreeding of Kurdi sheep with Romanov sheep did not show the desireble results.
Bizhan Mahmoudi; Hedayatollah Roshanfekr; Mohsen Sari; Mohammad Reza Bakhtiarizadeh
Volume 22, Issue 3 , September 2020, , Pages 337-348
Abstract
The objective of this study was to identify known intergenic lncRNAs related to biological pathways of acidosis in Holstein calves using ruminaltissue. Two groups of healthy calves (N=3) and affected by acidosis (N=3) were compared. Paired-end sequencing method was performed using theHiseq2500 illumine ...
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The objective of this study was to identify known intergenic lncRNAs related to biological pathways of acidosis in Holstein calves using ruminaltissue. Two groups of healthy calves (N=3) and affected by acidosis (N=3) were compared. Paired-end sequencing method was performed using theHiseq2500 illumine platform. Hisat2 software was used to align reads to the bovine reference genome and StringTie software package was used toassemble read files into transcripts. Using next generation sequencing, 1636 genes belonging to known intergenic lncRNAs were identified, of which56 genes showed significant differential expression (P≤0.05). Neighbor genes of known intergenic lncRNAs were determined on bovine genome.Analysis of biological pathways and molecular function showed that five biological pathways were significantly (P≤0.05) enriched. These pathwayswere Apelin signaling pathway, Gap junction, Glucagon signaling pathway, Renin secretion, and AGE-RAGE signaling pathway. Moreover, twomolecular functions including gap junction channel activity, and phosphatidyl inositol phospholipase C activity were significantly (P≤0.05) enriched.Some lncRNAs have different expression in healthy and acidosis samples, and the decreased pH acts as a stimulus to activate some biologicalsignaling pathways. In conclusion, it was indicated that lncRNAs with differential expression between the control group and the group affected byacidosis are associated with pathways related to rumen energy metabolism and signaling. Identified differentially expressed lncRNAs could be used asprognostic in acidosis and biomarkers or promising candidates in animal breeding.
elahe sanjari; mahdiyeh zieaadini; Masood asadi fozi; Ali Esmailizadeh koshkoiyeh
Volume 20, Issue 3 , November 2018, , Pages 365-373
Abstract
This wok was conducted to identify polymorphisms in the region of the exon 1 in 5'UTR of myostatin gene and their relationships with the growth traitsin Raeini cashmere goats. Myostatin gene has been identified as a factor causing a phenotype known as double-muscling in which a series of mutations render ...
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This wok was conducted to identify polymorphisms in the region of the exon 1 in 5'UTR of myostatin gene and their relationships with the growth traitsin Raeini cashmere goats. Myostatin gene has been identified as a factor causing a phenotype known as double-muscling in which a series of mutations render the gen inactivity; therefore, able to increase muscle growth. Blood samples were randomly taken from 150 Raeini cashmere goats. Total genomic DNA was extracted and a 701 bp fragment was amplified from the exon 1 of myostatin gene using PCR-RFLP method. The analysis of band patterns indicated three genotypes including AA, AB and BB with frequencies of 0.56, 0.33 and 0.11, respectively. The A and Balleles had a frequency of 0.72 and 0.28, respectively. The analysis of additive and dominance gene actions revealed that the effect of the myostatin gene on growth traits and body size of Raeini cashmere goats is via the dominance mechanism. The results showed that all growth traits except weaning weight were significantly associated with the identified polymorphisms, thus this gene can be used as a molecular marker in Raeini cashmere goats breeding.
Seyed Hamid Mousavi Esfiokhi; Mohammad Reza Sahlabadi; Behzad Khorrami
Volume 25, Issue 4 , December 2023, , Pages 375-388
Abstract
In order to investigate the chemical and nutritional characteristics of pea pod silage (PPS) and its effect on the performance of fattening lambs, 24 Zandi male lambs (initial BW, 33.5 ±1.5 kg) were used in a completely randomized design over 75 days. Experimental treatments were: 1) control; ...
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In order to investigate the chemical and nutritional characteristics of pea pod silage (PPS) and its effect on the performance of fattening lambs, 24 Zandi male lambs (initial BW, 33.5 ±1.5 kg) were used in a completely randomized design over 75 days. Experimental treatments were: 1) control; (basal diet) 2- pea pod silage (PPS replaced wheat straw and alfalfa in the basal diet). The amount of dry matter (DM), crude protein, ether extract, ash, neutral detergent fiber and acid detergent fiber of PPS were determined 20, 11.8, 1.8, 8, 62 and 20.2 percent, respectively, and pH was 6.02. The rumen degradability potential of DM and in vitro gas production potential of PPS after 96 h of incubation were estimated 83 and 58.6 percent, respectively. The use of PPS in fattening lambs rations improved the final body weight and increased the daily weight gain (P<0.05). The amount of DM digestibility was affected by the treatment (P<0.05), but digestibility of other nutrients was not affected by the experimental treatment. Blood parameters (cholesterol, triglyceride, HDL and LDL) were not affected by the experimental treatments, except for the glucose, which significantly decreased by feeding PPS (P<0.05). The experimental treatments had no significant effect on carcass traits. Based on the results of this study, the use of pea pod silage as a cost-effective alternative forage in replacement with common forages such as wheat straw and alfalfa can improve the performance of fattening lambs.
Mohammad Hossein Nemati; Seyed Abdoallah Hosseini; Ali Mansori; Seyed Saeid Mousavi
Volume 18, Issue 3 , October 2016, , Pages 387-397
Abstract
This study was conducted to rank the broiler farms based on buildings, installations and equipment and its effect on production factors. To determine the contribution of each factor in ranking of farms, a multi-criteria decision analysis (SAW) was used. Data of 108 broilers were recorded in specific ...
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This study was conducted to rank the broiler farms based on buildings, installations and equipment and its effect on production factors. To determine the contribution of each factor in ranking of farms, a multi-criteria decision analysis (SAW) was used. Data of 108 broilers were recorded in specific forms and the amount of investment per broiler was calculated for individual factors. To calculate the adjusted weights of parameters, 20 experts were consulted as was specified in forms. The results showed that ventilation (fans and air inlets), roof and wall insulation in a poultry houses have represented about 55 percent of technological factors. The breeding units holding rank 1 and 2 enjoyed high degree of mechanization. As the level of mechanization was improved, the density of chickens per unit area was also increased; These units used more tunnel ventilation and large fans, and their ceiling were insulated using fiberglass, polystyrene, corrugated plastic and thickness of walls were also 35 cm. Mostly external heaters have been installed. Feeding and drinker systems have exerted no important effect on production. Slaughter weight and survival percent were not affected by the degree of mechanization but feed intake and feed conversion ratio decreased and production index increased (p<0.01) in the breeding units holding rank 1 and 2. In general in poultry house, ventilation system and ceiling and wall insulation improved energy efficiency and increased the efficiency of the system.