Document Type : Research Paper

Authors

1 M.Sc. Student, Department of Animal and Poultry Sciences, College of Aboureihan, University of Tehran, Tehran, Iran

2 Assistant Professor, Department of Animal and Poultry Sciences, College of Aboureihan, University of Tehran, Tehran, Iran

Abstract

This study was done to gain insights into transcriptional regulation of negative energy balance (NEB) assoctiated genes. Overexpressed genes in NEB were identified using microarray and RNA-seq data and promoter analysis of these overexpressed genes was applied to identify novel transcription factors. Moreever, STRING database was used to construct a regulatory network of identified transcription factors. The results of the gene expression analysis revealed that eight genes in severe NEB are more frequent and significant (P<0.05) in comparison to the mild NEB. Promoter analysis showed that promoters of overexpressed genes are enriched in putative binding sites for 19 transcription factors. This group included known NEB-associated transcription factor (NF-κB), and a number of transcription factors (such as SP1, ZBP89, NFI, Zf9, MYC, ZBTB7A, FOXF2 and KLF6) that had not been previously reported to be associated with NEB. Based on the present results, 18 new effective candidate trsnacription factors introduced in this study can provide new information to gain a better understanding of the regulatory network involved in NEB.

Keywords

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