Shokufeh Hasanvand; Ali Khatibjoo; Hassan Shizadi; Yahya Mohamadi; Mohammad Amir Karimi Torshizi; Drakhshanda Rahimhi
Volume 25, Issue 3 , October 2023, , Pages 325-341
Abstract
Introduction: Exposure of poultry birds to extreme temperature stressor modulates the immune responsiveness and haemato-biochemical parameters of birds. Cold temperature can increase ascites susceptibility by increasing both metabolic oxygen requirements and pulmonary hypertension. Biochar is an ash ...
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Introduction: Exposure of poultry birds to extreme temperature stressor modulates the immune responsiveness and haemato-biochemical parameters of birds. Cold temperature can increase ascites susceptibility by increasing both metabolic oxygen requirements and pulmonary hypertension. Biochar is an ash substance that is produced from the burning of biological material via pyrolysis. This study was carried out to investigate the effects of wet Litter Biochar on performance of cold-stressed broiler chickens.
Material and Methods: In this experiment, the effect of wet litter biochar (WLB), probiotic and zeolite on performance, blood metabolites and small intestine morphology of broiler chickens reared under cold stress were investigated. In a completely randomized design 350 Ross-308 broiler chickens (one-day-old, mixed sexes) were allocated to seven treatments, five replicates and 10 birds in each replicate. Experimental treatments included: 1) positive control (basal diet in recommended temperature), 2) negative control (basal diet+ cold stress), 3) negative control+ %0.5 WLB, 4) negative control+ %0.75 WLB, 5) negative control+ 1% WLB, 6) negative control+ 0.02% probiotic Ecobacto-P, and 7) negative control+ 1% zeolite. In cold stress groups, house temperature decreased to 17° C from 7 d until end of the experiment (42 d).
Results and Discussion: As compared to group reared in recommended house, cold stress significantly decreased broiler chickens feed intake, body weight gain (BWG), carcass and abdominal fat percentages, serum triglyceride concentration, activity of aspartate transaminase and alanine aminotransferase enzymes as well as villous width of jejunum and ileum while increased total and ascitic mortality, ileal and jejunal villous height and crypt depth to villous length ratio (P<0.05). Inclusion of 0.5 and 1% WLB and probiotic increased broiler chickens BWG, ileal and jejunal villous length and surface area, but decreased feed conversion ratio as compared to negative control group (P<0.05).
Conclusion: Generally, although addition of wet litter biochar and probiotic led to improvement in performance and small intestine morphometry of cold-stressed broiler chickens, but they did not completely catch-up negative effects of cold stress on broiler chickens.
hamid amanlou; abolfazl zali
Volume 19, Issue 2 , August 2017, , Pages 337-348
Abstract
The aim of this study was to evaluate the effects of Pomegranate seed pulp (PSP) containing long-chain fatty acids with L-carnitine (LC) on performance and carcass traits of thirty two Mahabadi male kids. Experimental diets were 1) diet without PSP and LC 2) diet with 10% PSP Kg/DMI and without ...
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The aim of this study was to evaluate the effects of Pomegranate seed pulp (PSP) containing long-chain fatty acids with L-carnitine (LC) on performance and carcass traits of thirty two Mahabadi male kids. Experimental diets were 1) diet without PSP and LC 2) diet with 10% PSP Kg/DMI and without LC 3) diet without PSP and with 300ppm LC Kg/DMI and 4) diet with 10% PSP and 300ppm LC Kg/DMI. Kids were slaughtered at the end of study and carcass characteristics were measured. Addition of PSP and LC in diet decreased kidney fat (P<0.05). The kids fed with PSP and LC supplement had higher fat digestibility than other treatments (P<0.05). Furthermore, the use of PSP in the diet decreased the feed cost per Kg of hot carcass (P=0.05). The results of this study indicated that replacing a part of dietary cereal grains with PSP and LC supplement decreased kidney fat. Also, the simultaneous use of LC and PSP increased apparent digestibility of fat, and showed numerically higher performance. According to the results of this study, the PSP can be used as an economical byproduct without negative effects on performance, and along with LC in the diet of kids it will reduce kidney fat as well.
jamal mahmoudi; sara mirzaie goudarzi; Abbas Frahavar; Ali asghar Saki; Ali reza Nourian
Volume 21, Issue 3 , September 2019, , Pages 371-380
Abstract
The effect of essential oils blend, probiotic and flavomycin on performance, gastrointestinal tract (GIT) characteristics, cecal microbial population and blood parameters was studied with 288 day-old chickens, Ross 308 broilers from 1 to 42 days of age in a completely randomized design by 4 ...
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The effect of essential oils blend, probiotic and flavomycin on performance, gastrointestinal tract (GIT) characteristics, cecal microbial population and blood parameters was studied with 288 day-old chickens, Ross 308 broilers from 1 to 42 days of age in a completely randomized design by 4 treatments, 4 replicates and 18 chickens in each replication. The experimental treatments were: 1) basal diet as control group (without additive), 2) basal diet + 150 mg/kg essential oils blend, 3) basal diet + 200 mg/kg Gallipro® probiotic, and 4) basal diet + 150 mg/kg flavomycin. The results showed that feed intake was lower in the control group than other treatments (P<0.05). In the whole trial period, the weight gain of chickens fed with the diet containing flavomycin was higher than other birds and had a better feed conversion ratio than control birds or fed with the diet contains probiotic (P<0.05). The effect of treatments was not significant on relative weight of internal organs, pH of the GIT digesta and Lactobacillus and Escherichia coli population in the ceca. The relative weight of the breast was greater in chickens fed with flavomycin than control group or birds that received essential oils blend (P<0.05). There was no difference between treatments in terms of plasma cholesterol, glucose and triglyceride concentrations, but HDL concentration was lower in the plasma of birds receiving flavomycin compared to other treatments (P<0.05). Based on the results of this study, adding essential oils blend to the diet improved performance and can be used as a growth promoter instead of flavomycin in broilers diet.
Leila Taherabadi; Farokh Kafilzadeh
Volume 23, Issue 3 , September 2021, , Pages 387-394
Abstract
The aim of this study was to investigate the effect of Lactobacillus fermentum 92069 (LF) isolated from yogurt on chemical and microbial fermentation and aerobic stability of high moisture corn silage. After propagation and concentration determination LF was used to prepare experimental treatments with ...
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The aim of this study was to investigate the effect of Lactobacillus fermentum 92069 (LF) isolated from yogurt on chemical and microbial fermentation and aerobic stability of high moisture corn silage. After propagation and concentration determination LF was used to prepare experimental treatments with concentrations of zero (control, LF0), 1×106 cfu/g fresh forage (LF1) and 2×106 cfu/g fresh forage (LF2). Three replicates of each treatment were stored in laboratory silos for 90 days. The results showed that the chemical composition of silages (DM, NDF, ADF, CP, WSC) was not affected by addition of LF. LF2 had a significant lower pH compared to the control (P<0.05). LF1 and LF2 silages showed a higher concentration of lactic acid (P<0.05). Concentration of acetic acid increased and mold population decreased in LF2 compared to the other silages (P<0.05). There was no significant difference between population of lactic acid bacteria and yeast in silages. The aerobic stability of LF0 and LF1 silages decreased significantly compared to LF2 (P<0.05). During the aerobic stage after opening the silos, LF2 silage had the lowest pH (P<0.05). However, yeast population of silages during the aerobic stage was not affected by treatment. The results of this study showed that Lactobacillus fermentum 92069 has the potential to be used as a silage inoculant.
Hossien mohammadzadeh kratei; mohamad hossein shahir
Volume 22, Issue 3 , September 2020, , Pages 391-406
Abstract
The effects of increasing levels of L-leucine supplementation on performance, carcass traits, breast meat production and determination of digestible leucine requirement broilers during the grower period (15 to 30 days) using 240 Ross males broiler chickens in a completely randomized design with six dietary ...
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The effects of increasing levels of L-leucine supplementation on performance, carcass traits, breast meat production and determination of digestible leucine requirement broilers during the grower period (15 to 30 days) using 240 Ross males broiler chickens in a completely randomized design with six dietary treatments (five repetitions and eight chicks per repetition) was investigated. Treatments include; a basal diet with 1.53% digestible leucine and five diets containing 1.63, 1.73, 1.83, 1.93, and 2.03% digestible leucine by adding synthetic L-leucine to the basal diet. By increasing the levels of digetible Leucin, body wieght gain, carcass and breast muscle percentage were increased, but feed conversion ratio and abominal fat percentage were decreasd (p < 0.05). The response trends to increasing levels of digestible leucine were quadratic for weight gain, feed conversion ratio, the relative weight of carcass, relative weight of breast muscle and relative weight of abdominal fat ( p < 0.05). Due to the better fit of the quadratic model to the response criteria, digestible Leucine requirements were estimated at 1.70, 1.72, 1.724, and 1.735% for body weight gain, feed conversion ratio, the relative weight of carcass and breast muscle; respectively. Based on the average of these estimates, the suggested digestible Leucine requirements of broilers in the grower period is 1.72%.
Ali Kahyani; G. R. Ghorbani; M. Alikhani; E. Ghasemi; A. Sadeghi-Sefidmazgi; Sayyed Mahmoud Nasrollahi
Volume 20, Issue 3 , November 2018, , Pages 425-435
Abstract
The study was conducted to evaluate indigestible neutral detergent fiber (iNDF) and other related parameters of fiber digestibility of alfalfa hay, corn silage, wheat straw, beet pulp, and wheat bran of a dairy farm. Using two cannulated non-lactating Holstein cows and in situ method, undigested neutral ...
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The study was conducted to evaluate indigestible neutral detergent fiber (iNDF) and other related parameters of fiber digestibility of alfalfa hay, corn silage, wheat straw, beet pulp, and wheat bran of a dairy farm. Using two cannulated non-lactating Holstein cows and in situ method, undigested neutral detergent fiber (uNDF) after 288 h was measured as indicator of iNDF. Then with evaluating the kinetic of fiber digestibility in several time points, the rate and extent of NDF digestibility (NDFD) were measured according to newly-developed equations. The measured NDFD was used for estimation of total tract NDF digestibility (TTNDFD). Alfalfa hay had a greater amount of lignin as well as greater rate of NDF digestion than corn silage and wheat straw. Alfalfa hay and wheat straw, compared with corn silage, had a greater amount of uNDF and lower NDFD and TTNDFD. In spite of having relatively similar amount of NDF, the beet pulp and wheat bran had lower amounts of lignin and uNDF, while greater rate and extent of NDF digestibility as well as TTNDFD than forages. Overall, the considered fibrous feedstuffs showed a considerable variation in uNDF, NDFD, and TTNDFD that must be considered at the time of use.
Hoshang Lotfollahian; hossein manafi rasi; Abbas Najmabadi
Volume 25, Issue 4 , December 2023, , Pages 445-459
Abstract
To investigate the effects of using fennel root powder in the diet on the productive performance, immune responses, and meat quality of Arian broiler day-old chicks, an experiment was carried out in a completely randomized design with six treatments, four replicates and 20 (male and female) in each, ...
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To investigate the effects of using fennel root powder in the diet on the productive performance, immune responses, and meat quality of Arian broiler day-old chicks, an experiment was carried out in a completely randomized design with six treatments, four replicates and 20 (male and female) in each, for 42 days. The experimental groups include: 1- basic diet (control) 2- basic diet + 2 g/kg fennel root powder 3- basic diet + 4 g/kg fennel root powder 4- basic diet + 6 g/kg fennel root powder 5- basic diet + 250 mg/kg antibiotic and 6- basic diet + 200 mg/kg probiotic. The live weight , livability percentage , the production index and carcass characteristics were not affected by the experimental treatments during the whole period. In contrast, the average feed consumption in the whole period was affected by the treatments containing fennel and antibiotics (P<0.05). The antibodies against sheep red blood cells (SRBC) and immunoglobulin G were higher in the treatments using fennel root powder compared to the control treatment (P<0.05). The use of fennel root powder at all levels, reduced MDA after seven days of keeping meat in the refrigerator at 4 degrees Celsius (P<0.05). In conclution, regarding the positive effects of using fennel root powder on the immune responses and meat preservation of broilers, it is recommended to use it at the rate of 2 grams per kilogram of diets.
Yazdan Seyf; Farokh Kafilzadeh; Hassan khamisabadi; Massoumeh Rezaei; Leila Taherabadi
Volume 24, Issue 4 , December 2022, , Pages 453-462
Abstract
The effect of feeding essential oil of Pistacia Atlantica Kurdica gum during the suckling period on pre and post-weaning growth performance of Sanjabi male lambs using 30 male lambs from an estrus synchronized flock of ewes was carried out, in the form of a completely randomized design with 3 treatments ...
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The effect of feeding essential oil of Pistacia Atlantica Kurdica gum during the suckling period on pre and post-weaning growth performance of Sanjabi male lambs using 30 male lambs from an estrus synchronized flock of ewes was carried out, in the form of a completely randomized design with 3 treatments and 10 replications. The lambs were divided into three groups three days after birth. The first group received no essential oil, and the second and the third groups received a daily dose of 0.8 and 1.6 ml until weaning by a drenching syringe, respectively. Lambs were weaned at 100 days of age and were raised for 10 weeks with the other herd lambs until 170 days of age. Feeding the essential oil increased weaning weight and post-weaning daily weight gain. The blood glucose of lambs during the suckling or post-waning period did not change due to feeding the essential oil. Lambs received the essential oil and had lower blood cholesterol, triglyceride, total protein, and higher urea and albumin to globulin ratio. Feeding the essential oil reduced blood malondialdehyde levels but increasing the level of the essential oil did not further decrease this metabolite. The results showed that the essential oil as a new feed additive has a good potential to improve the pre and post-weaning performance of lambs when fed during the suckling period. Since, increasing the level of the essential oil resulted in no additional improvement in the performance of lambs therefore, a daily dose of 0.8 ml is recommended.
Mohammad Heidarian; JAvad Bayad Koohsar; Youssef Moostafloo; Balal Sadeghi; Farid Moslemipoor
Volume 18, Issue 3 , October 2016, , Pages 461-475
Abstract
In order to compare the effects of different weaning strategies on feed intake, structural growth measurement and health, blood metabolites and economic efficiency of young dairy calves, twenty four female Holstein calves were used. Calves after birth were randomly assigned into one of three weaning ...
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In order to compare the effects of different weaning strategies on feed intake, structural growth measurement and health, blood metabolites and economic efficiency of young dairy calves, twenty four female Holstein calves were used. Calves after birth were randomly assigned into one of three weaning strategy as follow: 1) weaning based on feed intake, 2) weaning based on weight gain (30 kg weight gain compared to the birth weight), and 3) weaning based on age (at 90 days of age). Starter intake measurement and fecal scoring conducted daily. Calves were weighed weekly and blood samples were obtained on days 21, 42, 60 and 90 after birth. Results showed that there were significant differences among different weaning strategies on average weaning age and milk consumption (p<0.05). All treatments had similar average daily weight gain, average daily feed intake, final weight and structural growth measurements. Calves weaned based on feed intake had lowest ruminal pH and N-NH3 concentration and blood urea nitrogen on day 90 (p<0.05). There were differences between groups on milk cost and total cost (milk + feed) and feed cost per kg of BW gain, so that Weaning based of feed intake compared to weaning based on weight and age saved up to 6000 and 3000 Rials per kg of body weight, respectively. Generally, similar growth and performance among different weaning strategies as well as reduction the cost of raising of calves indicated that calves weaning based on feed intake can be profitable approach.
Seyed Morteza Vaghar Seyedin; Mohsen Mojtahedi; Mohhamad Hasan Fathi Nasri; Seyed Ehsan Ghiasi
Volume 21, Issue 4 , January 2020, , Pages 487-497
Abstract
This study aimed to evaluate the buffering capacity of some buffer mixtures and the effect of their inclusion in the diet on in vitro ruminal fermentation parameters. For this purpose, five buffer mixtures were prepared as: 1) containing 15% sodium bicarbonate + 15% magnesium oxide + 35% magnesium ...
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This study aimed to evaluate the buffering capacity of some buffer mixtures and the effect of their inclusion in the diet on in vitro ruminal fermentation parameters. For this purpose, five buffer mixtures were prepared as: 1) containing 15% sodium bicarbonate + 15% magnesium oxide + 35% magnesium carbonate + 35% sodium bentonite; 2) containing 35% sodium bicarbonate + 35% magnesium oxide + 15% magnesium carbonate + 15% sodium bentonite; 3) containing 20% sodium bicarbonate + 40% magnesium oxide + 20% magnesium carbonate + 20% sodium bentonite; 4) containing 40% sodium bicarbonate + 20% magnesium oxide + 20% magnesium carbonate + 20% sodium bentonite; and 5) containing 16% sodium carbonate + 35% magnesium oxide +22% magnesium carbonate + 5% sodium bentonite and 22% monobasic potassium phosphate. Buffering capacity and buffer value index were measured. Then the effect of buffer mixtures on gas production potential, pH, ammonia nitrogen concentration and in vitro dry matter disappearance (IVDMD) was investigated. Bufferingcapacity and buffer value index of buffer mixture 2 was higher than other buffer compounds (P<0.05). Buffer mixture 4 increased gas production (P<0.05). IVDMD was the same between the buffer mixtures and only buffer mixture 4 and 1 were different (P<0.05). The use of buffer mixture 2 increased the pH of the batch culture (P<0.05). Overall, the results of this experiment showed that the use of buffer mixture2 could have an effective role in preventing pH drop and consequently reducing acidosis.
Mohammad Hossein nemati; Mustafa Hajilou; sayed abdoullah hosseini; seyyed saeid mousavi
Volume 23, Issue 4 , January 2022, , Pages 549-559
Abstract
In this research the effect of Bioplus B2 probiotic and galactooligosaccharide prebiotic (GOS) on performance, egg quality, and some blood parameters of commercial laying hens were investigated in a sample of 216 hy-line laying hens (w36) with the same average weight aging 50 to 62 weeks for a period ...
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In this research the effect of Bioplus B2 probiotic and galactooligosaccharide prebiotic (GOS) on performance, egg quality, and some blood parameters of commercial laying hens were investigated in a sample of 216 hy-line laying hens (w36) with the same average weight aging 50 to 62 weeks for a period of 12 weeks in a completely randomized design with 6 treatments, 6 replications and 6 hens per each replication in the cage system. Experimental treatments included 1) without additives (as control), 2) diets containing 0.1% Bioplus B2 probiotic, 3) to 6) diets; containing, 0.05, 0.1, 0.15 and 0.20 % of galactooligosaccharides prebiotic, respectively. The results indicated that both egg weight and mass was increased significantly (P<0.05) in the Bioplus B2 probiotic treatment. Egg production percentage, feed intake and feed conversion ratio were not significantly influenced by treatments. Using galactooligosaccharide at the levels of 0.05, 0.1 and 0.2 percent improved dry eggshell weight significantly while the levels of 0.05, 0.15 and 0.2 percent increased eggshell weight per unit area compared to the control (P<0.05). Total protein and albumin of serum increased in the Bioplus B2 treatment (P<0.05). Using Bioplus B2 probiotic and 0.15% galactooligosaccharide significantly decreased blood cholesterol level (P<0.05). In general, the results showed that the using of prebiotic galactooligosaccharide at the level of 0.15 % increases the weight of eggshell per unit area, decreases the blood serum cholesterol, and improves some performance traits compared to control group, therefore it can be used instead of probiotic Bioplus B2.
Amin Saeid; Mohsen Afsharmanesh; Mohamad Salarmoini
Volume 22, Issue 4 , December 2020, , Pages 571-581
Abstract
The effect of copper-methionine chelate and oregano essential oil in comparison to antibiotics on performance, meat quality, immune response and blood biochemical parameters were evaluated using 240 Ross 308 broilers in a 2×2×2 factorial experiment in a completely randomized design with four ...
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The effect of copper-methionine chelate and oregano essential oil in comparison to antibiotics on performance, meat quality, immune response and blood biochemical parameters were evaluated using 240 Ross 308 broilers in a 2×2×2 factorial experiment in a completely randomized design with four replicates and 10 birds per replicate. The treatments included three incremental levels (No additives, 500 mg/kg flavofospholipol antibiotics and 100 mg/kg oregano essential oil of diet) and copper-methionine chelate (0 and 118 mg/kg diet). At the end of the period (42th day), one male bird was sampled from each replicate for exploring meat quality, immune response and blood biochemical parameters. Birds fed a diet containing coppermethionine chelate with flavophospholipol showed higher live weight (P<0.05). Birds fed a diet containing copper-methionine chelates had higher weight gain and better conversion ratio (P<0.05). Also, birds fed diets without additives and antibiotics with and without copper-methionine chelates had more fatty acid oxidation than other groups (P<0.05). At 28 and 42 days of age, the highest immune response (antibody titer) was observed in the copper-methionine chelate group with oregano essential oil (P<0.05). It was shown that oregano essential oil decreased and increased LDL and HDL in the blood (P <0.05), respectively. The results of this study showed that the use of copper-methionine chelate with antibiotics improved production efficiency and the use of copper-methionine chelate and oregano essential oil supplements in poultry diets improve meat quality and increased safety.
Heydar Zarghi; َAbolghasem Golian; Ahmad Hassanabadi; Farhad Khaligh
Volume 20, Issue 4 , February 2019, , Pages 577-587
Abstract
The present study was conducted to determine the zinc (Zn) requirement in broiler chickens fed wheat-soy based diet. Two hundred fifty day-old broiler chicks (Ross 308), were randomly assigned to a completely randomised design (CRD) experiment with 5 treatments, and 5 replicates of 10b each. The experimental ...
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The present study was conducted to determine the zinc (Zn) requirement in broiler chickens fed wheat-soy based diet. Two hundred fifty day-old broiler chicks (Ross 308), were randomly assigned to a completely randomised design (CRD) experiment with 5 treatments, and 5 replicates of 10b each. The experimental treatments included five dietary Zn levels (30, 70, 110, 150, and 190 mg/kg) provided by the addition of reagent grade “ZnSO4.7H2O”. The experiment lasted from 1-42d of age. Increasing dietary Zn level to 70mg/kg significantly increased 10, 24 and 42d live body weight (LBW), feed intake (FI) and weight gain (WG) during 1-10, 25-42 and 1-42d of age and feed efficiency (FE) during 1-10d of age. The linear broken-line (LBL), quadratic-broken line (QBL) and the quadratic polynomial (QP) regression models were used to estimate the Zn requirement. The Zn requirement for optimal final LBW and FI, WG, and FE during 1-42d of ages by LBL model were 68.77, 83.12, 66.99 and 62.75 mg/kg of diet, and by QBL model 70.00, 79.93, 69.63 and 58.01 mg/kg of diet, respectively. In conclusion, Zn requirement varied between 58-83 mg/kg, which is higher than NRC and is lower than strain recommendation in the broiler chickens fed wheat-based diet.
parviz ordouny; khalil mirzadeh; tahereh mohammadabadi; mohammad bojarpoor
Volume 19, Issue 3 , November 2017, , Pages 601-612
Abstract
To evaluate the effect of different levels of wild pistachio leaves on liver enzymes, bloodparameters and performance indicators of broiler chickens, 192 one-day old Ross 308 broiler chickens in the completely randomized design, including 4 treatments, 4 replicates and 12 chicks (both sexes), per ...
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To evaluate the effect of different levels of wild pistachio leaves on liver enzymes, bloodparameters and performance indicators of broiler chickens, 192 one-day old Ross 308 broiler chickens in the completely randomized design, including 4 treatments, 4 replicates and 12 chicks (both sexes), per experimentalunits were used. The experimental treatments includedrationscontaining levelsofzero (control), 1, 2, and 3 percent of wild pistachio leaf powder. The results of this experiment showed that effect of wild pistachio leaf on feed intake and daily gain significantly reduced compared to control treatment in the initial period (P˂0.05), but feed intake and daily gain were not significant in the growth period and total period.The feedconversion ratio was not significantly affected (P > 0.05). The effect of experimental treatments was not significant on AST and ALT, but the ALP enzyme was significantly reduced by the experimental treatments (P˂0.05). The amount of glucose, total cholesterol and HDL cholesterol of serum were not significantly affected. The effect of experimental treatments on triglyceride and LDL cholesterol serum reduction was significant (P˂0.05). According to the results, wild pistachio leaf can be used as an additive plant with reducing feature on liver enzymes and triglyceride and LDL cholesterol in poultry diets.
Volume 18, Issue 4 , December 2016, , Pages 711-718
Abstract
A total of 96 semen samples were collected from Sanjabi breed sheep over two years and during autumn and spring seasons in order to investigate the leptin gene polymorphism and its relationship with the characteristics of sperm quality and testicular size. At the same time, the dimensions of length, ...
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A total of 96 semen samples were collected from Sanjabi breed sheep over two years and during autumn and spring seasons in order to investigate the leptin gene polymorphism and its relationship with the characteristics of sperm quality and testicular size. At the same time, the dimensions of length, width and scrotal circumference were measured and blood samples were taken from sheep jugular vein for extract DNA. PCR was performed using specific primers to amplify a 463bp fragment including exon 3 of leptin gene. After PCR and determining three GG, GT and TT genotypes in the studied samples, it was determined that leptin gene polymorphism had significant relation with individual motility (P<0.05) and motility spermatozoa in animals with GG genotype had the highest. sperm volume, sperm concentration, total sperm ejaculated, hypo osmotic swelling test (HOS), viability and sperm index, in animals with GG genotype had the highest (P<0.05). In animals with TT genotype mean of length and scrotal circumference and in GT genotypes also testicular width and volume were more compared to GG genotypes. These results showed of this leptin gene polymorphism in order to improving fertility in breeding programs of Sanjabi sheep had high heterozygosity, it seems that more studies in larger populations are needed to better understand the relation of other leptin gene polymorphisms on ram fertility.
hossein manafi rasi; Hossein Heidary chah baghi; salman Afshar; Narges Afshari pour
Volume 19, Issue 4 , March 2018, , Pages 819-828
Abstract
This study was carried out to investigate the effect of Ferulla alliacea resin and wire copper oxide on internal parasite control as well as growth performance of Baluchi fattening lambs. Towenty-five Baluchi lambs, aged 6 to 7 months and weighed 20 ± 5 kg on average, were selected as trial sample. ...
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This study was carried out to investigate the effect of Ferulla alliacea resin and wire copper oxide on internal parasite control as well as growth performance of Baluchi fattening lambs. Towenty-five Baluchi lambs, aged 6 to 7 months and weighed 20 ± 5 kg on average, were selected as trial sample. The experiment was done using completely randomized design with five groups as treatments replicated five times for 60 days. Treatments consisted of 1) control ( without additive), 2) Ferulla alliacea resin, 3) copper oxide wire, 4) combination of copper and Ferulla alliacea resin, 5) albendazole (anti parasite druge). During the experiment period, some parameters such as EPG (the number of eggs per gr of feces) and PCV (hematocrit equivalent) were measured at 0, 15, 30, 45 and 60 day of experiment, as well as weight gain weekly. According to the results, body weight changes were significant in 3th, 4th, 5th and 7th weeks (p<0.05). No significant difference was observed for PCV at all times. In addition, it appeared that copper oxide, Ferulla alliacea resin and albendasole had the same effect on decreasing EPG on 60th d. In addition,. The combination of Ferulla alliacea resin and copper oxide effectively reduced EPG in a shorter time (45th d) . The findings showed that Ferulla alliacea and copper oxide can be used as control of internal parasites in sheep, especially in organic systems.
Hossein Mohammadi; mohammad Shamsollahi
Articles in Press, Accepted Manuscript, Available Online from 23 November 2023
Abstract
The aim of the present study genome wide association studies based on Gene set enrichment analysis for identifying the loci associated with related to fleece traits in Cashmere goat using the high-confidence SNPs. For this purpose, the 192 Cashmere goats were performed with fiber length, fiber diameter ...
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The aim of the present study genome wide association studies based on Gene set enrichment analysis for identifying the loci associated with related to fleece traits in Cashmere goat using the high-confidence SNPs. For this purpose, the 192 Cashmere goats were performed with fiber length, fiber diameter and cashmere yield in GEMMA software. Using the biomaRt2 R package the SNP were assigned to genes. Finally, a gene enrichment analysis was performed with the KOBAS platform from online bioinformatics databases. Bioinformatic analysis showed that the identified genomic regions directly and indirectly overlap with genes affecting the production of keratinocytes, epidermal growth and development, hair follicle growth and collagen. According to pathway analysis, 18 pathways from gene ontology and KEGG pathway were associated with the quantity and quality wool trait (P˂0.05). Among those pathways, the Oxytocin signaling pathway, positive regulation of intracellular protein transport and epidermal cell differentiation has an important role in the hair follicle development, keratinocytes differentiation and development of epidermal. Also, the MAPK signaling pathway, cellular response to hormone stimulus and Insulin secretion significant association with quantity wool traits. Using these findings could potentially be useful for genetic selection in the breeding programs and can be used to understand the genetic mechanism controlling this trait. However, it will be necessary to carry out more association and functional studies to demonstrate the implication of genes obtained from association analyses. Using these findings can accelerate the genetic progress in the breeding programs with the aim of producing fine fibers.
Volume 15, Issue 1 , July 2014, , Pages 1-7
Volume 15, Issue 2 , October 2013, , Pages 1-7
Volume 16, Issue 1 , May 2014, , Pages 1-7
Hossein Karimi; Arash Azarfar; Heshmatollah Khosravinia; Ali Kiani
Volume 17, Issue 1 , April 2015, , Pages 71-82
Abstract
Our objective was to examine the effect of inclusion of dried de-oiled savory (Satureja khuzistanica; DDS) on production performance and fatty acids composition of longissimus muscle (LM) of finishing lambs. 30 Farhani lambs with average body weight of 33.6±1.4 kg were used. The lambs were randomly ...
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Our objective was to examine the effect of inclusion of dried de-oiled savory (Satureja khuzistanica; DDS) on production performance and fatty acids composition of longissimus muscle (LM) of finishing lambs. 30 Farhani lambs with average body weight of 33.6±1.4 kg were used. The lambs were randomly divided into five groups and individually fed with the experimental diets. In the experimental diets, alfalfa was replaced by dried de-oiled savory at the levels of zero (control diet), 25, 50, 75 and 100 percent. The lambs were fed whit finishing diets for 60 days following a 15-day adaptation period. During the finishing periods the lambs were weighed individually every 15 days and average daily gain, average feed consumption and feed conversion ratio were calculated. At the end of experiment, the lambs were slaughtered. Samples of LM along with subcutaneous fat were taken from the region of 12th and 13th ribs post-mortem and their fatty acids profile were determined by gas chromatography. The results of current study showed that the experimental diets had no effect on production parameters of lambs assessed. Saturated fatty acids content of LM was higher in the lambs fed with diets in which alfalfa was replaced by DDS at the level of 25 percent than those fed with diets in which alfalfa fully replaced by DDS (P<0.05). The lambs fed on diets in which alfalfa was replaced by DDS at the levels higher than 25 percent had numerically higher saturated fatty acids in their LM compared to control diet-fed lambs. The results showed that dietary inclusion of DDS at the level of 75 percent instead of alfalfa numerically increased poly-unsaturated fatty acids content of LM. Dietary replacement of alfalfa with DDS at the levels of 25 to 75 percent numerically lowered the proportion of omega-6 to omega-3 fatty acids of LM compared to the control diet. Results showed that Alfalfa can be replaced by dried de-oiled savory in finishing lambs diets and improve meat quality by reducing sits saturated fatty acids content.
hossein pasha zanussi; farid shariatmadari; Mohsen sharafi; Hamed Ahmadi
Volume 22, Issue 1 , March 2020, , Pages 79-92
Abstract
This study was conducted to assess the effects of dietary flaxseed and sesame oils, on the semen parameters, fatty acid composition of sperm as well as the fertility and hatchability eggs from aged roosters. In a completely randomized design, 24 Ross-308 roosters (aged 45 week) assigned to ...
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This study was conducted to assess the effects of dietary flaxseed and sesame oils, on the semen parameters, fatty acid composition of sperm as well as the fertility and hatchability eggs from aged roosters. In a completely randomized design, 24 Ross-308 roosters (aged 45 week) assigned to four groups, comprising six replicates and one bird in each. The birds received different diets including basal diet (control), basal diet supplemented with 2% flaxseed oil (FO), basal diet supplemented with 2% sesame oil (SO) and basal diet supplemented with 1% flaxseed oil and 1% sesame oil (MO). The diets were iso-caloric and iso-nitrogenous, containing the same level of vitamin E. The roosters were fed diet for 60 days, during which semen samples were collected on 1st, 20th, 40th and 60th days and the samples were tested for different characteristics. The results indicated that different diets affected semen qualities, except semen volume and the morphology. The concentration, progressive motility, MDA as well as viability of sperms were significantly different during different times of the experiment. The sperm quality parameters including total and progressive motility as well as MDA turned out to improve in the roosters fed FO or MO. Furthermore, the integrity of sperm membrane, DHA and DPA concentration, as well as the fertility were higher in the treatment group containing FO. It seems that supplementation of aged rooster’s diet with flaxseed oil or mixed oils, together with vitamin E improves the semen qualities and it can be applied as an appropriate strategy to preserve the reproductive performance of aged rooters.
Leila Mirisakhani; Hamid Reza Taheri
Volume 24, Issue 1 , April 2022, , Pages 81-95
Abstract
Effect of different levels of calcium (0.6 and 0.3%), phytase (0 and 1500 FTU/kg), and citric acid (0 and 1%) in diets containing 0.15% of non-phytate phosphorus, on growth performance, carcass traits, and bone chemical properties were investigated using 882 10-day-old male broilers from 11 to 40 days ...
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Effect of different levels of calcium (0.6 and 0.3%), phytase (0 and 1500 FTU/kg), and citric acid (0 and 1%) in diets containing 0.15% of non-phytate phosphorus, on growth performance, carcass traits, and bone chemical properties were investigated using 882 10-day-old male broilers from 11 to 40 days of age in 2 × 2 × 2 factorial arrangements based on a completely randomized design including nine treatments (positive control and eight diets without any inorganic P) and seven replications. phytase supplementation increased average daily gain and gain to feed ratio, but the effect of phytase was more apparent in broiler chickens received diets containing 0.6% calcium at 25 to 40 days of age (P <0.01). Reducing dietary calcium levels decreased average daily gain (11 to 24 days) and gain to feed ratio (11 to 24 and 25 to 42 days). Also, the inclusion of citric acid in diets containing 0.3% calcium had a negative effect on these traits (P <0.01). The combination of phytase and citric acid in diets containing 0.6% Ca increased the feed efficiency compared to the 0.3% diet (P <0.01). Phytase supplementation increased toe ash and bone sodium percentage and decreased bone calcium and phosphorus concentration (P<0.01). Bone phosphorus decreased by reducing dietary calcium levels and phytase supplementation (P <0.01). The relative heart and pancreas weight decreased with phytase supplementation (P<0.05). Reducing dietary calcium levels increased the relative liver and pancreas weight and decreased abdominal fat and heart percentage (P<0.05). According to the results, the combination of phytase and citric acid in diets containing 0.6% calcium was more effective in improving growth performance of broilers fed diets containing low non-phytate phosphorus.
Leila Taherabadi; Farokh Kafilzadeh
Volume 25, Issue 1 , April 2023, , Pages 83-91
Abstract
The present study was conducted in order to determine yield and the nutritive value of Napier grass at different growth stages as a new forage source in Iran. For this purpose, Napier grass was harvested at three stages of vegetative growth (65, 85 and 115 days after planting, respectively). Chemical ...
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The present study was conducted in order to determine yield and the nutritive value of Napier grass at different growth stages as a new forage source in Iran. For this purpose, Napier grass was harvested at three stages of vegetative growth (65, 85 and 115 days after planting, respectively). Chemical composition, rumen fermentation parameters, yield and some morphological traits were investigated. At the final growth stage, dry matter (DM) yield and height of Napier grass was 26.8 tons per hectare and 257 cm, respectively. With increasing age of the plant, a decrease in crude protein and an increase in lignin of cell wall was observed (P < 0.05). Crude protein and neutral detergent fiber content of the grass at this stage were 88 and 63 g/kg DM, respectively. Changes in chemical compositions during growth stages were more pronounced in the stem than in the leaf. During the growth period, the decrease in digestibility of leave and stem led to a decrease in dry matter and organic matter digestibility of Napier grass from 782 to 649 and from 814 to 747 g/kg DM, respectively (P < 0.05). The amount of gas production potential decreased with increasing plant age (P < 0.05). Fermentation rate and lag time were similar in plant harvested at the three stages of growth. The results of the present study showed that Napier grass with high yield of digestible organic matter and high nutritive value can be considered as a valuable source of forage in the country.
Ali Reza Amouyan Khaveh; Mohammad Amir Karimi Torshizi; Farid Shariatmadari
Volume 18, Issue 1 , April 2016, , Pages 85-94
Abstract
Effect of intermittent mash-pellet feeding programs on broilers performance, ascites susceptibility and economic yield was studied using 480 broilers in six treatments and five replicates of 16 chicks per pen were used in a completely randomized design. Treatments including: 1. Mash feed, 2. Heated-mash ...
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Effect of intermittent mash-pellet feeding programs on broilers performance, ascites susceptibility and economic yield was studied using 480 broilers in six treatments and five replicates of 16 chicks per pen were used in a completely randomized design. Treatments including: 1. Mash feed, 2. Heated-mash feed, 3. Mash feed in starter and grower and pellet feed in finisher, 4. Mash feed in starter and pellet feed in grower and finisher, 5. Pellet feed, 6. Grinded-pellet feed. Pellet feeding increased the feed intake and body weight gain and improved the feed conversion ratio, although the ascites susceptibility increased (P<0.01). Mash feeds decreased the body weight gain and increased the feed conversion ratio (P<0.01). Feeding pellet in finisher resulted in the same feed conversion ratio with feeding pellet for whole period or grower and finisher periods, however feed conversion ratio in pellet fed birds was better than mash fed birds (P<0.01). Feeding pellet for whole period is necessary to obtain maximum feed intake and body weight gain. Feeding mash feed in the first four weeks or whole period reduced the ascites susceptibility (P<0.01). The ascites susceptibility increased by increase in pellet feeding length (P<0.01). Feeding pellet diets reduced the feed cost for a kg live weight production and the increased gross profit compared to mash diets (P<0.05). In order to use the benefits of pellet feed and avoid high mortality rates and ascites susceptibility, it is recommended to start with mash feed and fed pellet in growing and finisher periods.