Volume & Issue: Volume 15, Issue 2, Autumn 2013, Pages 79-148 

Logistic analysis of some factors affecting on incidence probability of milk fat depression in early lactation of Iranian Holstein cows

Pages 79-88

https://doi.org/10.22059/jap.2013.51384

Maryam Moghadam Mousaabadi, Homayoun Farhangfar, Moslem Bashtani, AliReza Eghbal

Abstract The objective of the present research was to apply logistic analysis for investigating some factors
affecting incidence probability of milk fat depression (MFD) in early lactation of Iranian Holstein cows.
The data set was comprised 80381 test day records (milk, fat and protein traits) belonging to 80254 first
parity cows in 427 herds with calving year between 1995 and 2009. Based on fat to protein percentages
for the first month of the lactation and also with respect to threshold point of -0.12, dependent variable
was defined as a dichotomous (zero code for lack of MFD and unit code for MFD) variable. A logistic
mixed generalized linear model was fitted on the data using GLIMMIX procedure of SAS software. In
the model, fixed effects of sperm type (local or exotic), production season, first calving age, cow type
(grade or pure Holstein) and random effect of animal’s sire were included. Cow type had no significant
affect on MFD. Estimation of odds ratio for comparing different ages was found to be 1.112 indicating
that cows calving at lower age of 25 months are expected to show MFD by 11.2 percent smore than the
cows calving at the greater ages. The estimate of odds ratio for comparing of spring with summer, autumn
and winter seasons were 0.73, 0.722 and 1.182, respectively. The corresponding figures for summer as
compared to autumn and winter seasons were 0.987 and 1.615, respectively, and autumn as compared to
winter was found to be 1.636. Daughters of the local bulls are expected to show MFD by 10.8 percent
more than that of the daughters of the foreign bulls.

Microsatellite mapping of quantitative trait loci associated with carcass traits on chromosome 1 in Japanese quail

Pages 89-99

https://doi.org/10.22059/jap.2013.51386

Hasan Moradian, Ali Esmailizadeh, Mohammadreza Mohammadabadi

Abstract The purpose of this study was to identify genomic regions of quantitative trait loci (QTL), affecting
carcass traits on chromosome 1 in an F2 population of Japanese quail. For this purpose, a three-generation
resource population was developed by using two distinct Japanese quail strains, wild (meat type) and
white (layer type). Eight pairs of white and wild birds were crossed reciprocally and 34 F1 birds were
produced. The F1 birds were intercrossed to generate 422 F2 offspring. Phenotypic data including weight
of carcass parts were collected on F2 birds. All of the animals from three generations (472 birds) were
genotyped for eight microsatellite markers on chromosome 1. QTL analysis was performed with least
squares interval mapping method fitting three various statistical models. Significant QTL were identified
for breast weight, carcass weight, head weight and percentage of breast. There was also evidence for
imprinted QTL affecting breast weight, a carcass part of high economic value, on chromosome 1. The
proportion of the F2 phenotypic variation explained by the significant additive, dominance and imprinted
QTL effects ranged from 1.8 to 2.3, 1.2 to 3.3 and 0.5 to 2.2 percent, respectively.

Polymorphism of exon 2 of bone morphogenetic protein 15 gene (BMP15) and its relationship with litter size in Najdi goat

Pages 101-107

https://doi.org/10.22059/jap.2013.51387

Elham Javdan, Jamal Fayazi, Saleh Tabatabaei, Mohammadtaghi Baigi Nasiri

Abstract This study was conducted to detect polymorphism of gene BMP15, a member of Transforming Growth
Factor β (TGFβ) family which has a crucial role in controlling the ovarian follicles development,
ovulation rate and fertility. Samples were randomly selected from 91 Najdi goats in 3 geographical
locations, northwest, southeast and center of Khuzestan province. After DNA extraction, amplification of
235 bp fragment of exon 2 of BMP15 gene was performed using specific primers. Sequence detection
was executed after amplification of gene fragments. The association of BMP15 gene and litter size was
done by SAS software. Results from sequencing were analyzed by Vector NTI software. The results
identified three mutations in bases 529 (T to G), 530 (C to G) and 576 (T to C). The largest litter size
belongs to AA pattern. Point mutations in this gene will alter the Ovulation rate of the goat. Therefore,
improving twining trait in Najdi population can be expected by marker assisted selection.

The effect of different zinc sources on performance, blood mineral and cell counts of Zandi lambs

Pages 109-115

https://doi.org/10.22059/jap.2013.51388

Mokhtar Malaki, Mohammadali Norouzian, Aliakbar Khadem

Abstract This research was conducted to evaluate the effect of different zinc (Zn) sources on performance, blood
mineral and cell counts of Zandi lambs. 18 male lambs at weaning were supplemented with two different
sources of zinc, 25 ppm ZnSO4 and 25 ppm zinc-peptide added to the basal diet as control. Live body
weight, weight gain and feed efficiency improved with Zn-peptide supplementation (P<0.05). Sera Zn
concentration was higher and copper and iron concentration was lower in zinc supplemented groups
(P<0.05). Supplementation of zinc had no effect on blood cell counts except for white blood cells that
decreased with zinc peptide supplementation (P<0.05). It was concluded that adding 25 ppm zinc peptide
to fattening lamb diet improved performance and cause to higher level of blood zinc.

Effect of lecithin and bile salt on performances, digestibility and morphology of broiler chickens

Pages 117-126

https://doi.org/10.22059/jap.2013.51389

Fereshteh Jamili, Farid Shariatmadari, Mohammad-amir Karimi Torshizi

Abstract This experiment was conducted to evaluate the effect of emulsifiers such as lecithin and bile salts on
broilers performances fed diet containing vegetable fats. 192 male Ross broiler chickens were randomly
allocated in a factorial experiment design of 3 × 2, consisting two sources of fat (four percent fatty acids
or soy bean oil) and three emulsifiers (none, one percent lecithin and 0.05 percent bile salts) in
completely randomized design with four replicates and eight birds per each. Total weight gain and feed
conversion ratio was not affected by fat sources while soya oil addition to diets increased feed intake
(P<0.05). Emulsifiers’ addition to diet improved weight gain feed conversion ratio (P<0.05). The length
and width of villi in jejunum, and crypt depths in duodenum were not affected by fat sources, while, crypt
depth in jejunum and width of villi in ileum of birds fed on diets containing fatty acid were higher than
other birds (P<0.05). Addition of emulsifiers to diets increased dietary metabolisable energy (P<0.05). In
conclusion, addition lecithin or bile salts to diets containing soybean oil or its fatty acids, improved body
weight gain and feed conversion ratio in broiler chicks by increasing in absorption area of intestine and
dietary metabolisable energy.

Effect of different levels of silymarin on performance, carcass characteristics and antioxidant status in broiler chickens

Pages 127-138

https://doi.org/10.22059/jap.2013.51390

Rohollah Ebrahimi, Tahereh Mohammadabadi, Mohsen Sari, Somayeh Sallari, Mohammadjavad Zamiri, Mohammadtaghi Beigi Nassiri

Abstract In order to investigate the effect of different levels of silymarin (0, 100 and 200 mg/kg diet) on
performance, carcass characteristics, blood parameters and antioxidant status, 120 day-old chicks were
assigned to 3 treatments with four replicates and 10 birds in each in a completely randomized design.
Results showed supplementation of 200 mg silymarin significantly decreased the body weight gain
(BWG) in starter (P<0.05) and whole period of experiment (P<0.01) and significantly increased feed
conversion ratio at starter (P<0.05), grower (P<0.05) and total rearing periods (P<0.01). Also, addition of
100 mg silymarin significantly increased the carcass relative weight (P<0.01) and supplementation of 200
mg silymarin significantly increased the breast relative weight (P<0.05). Furthermore, 200 mg silymarin
significantly increased the malondialdehyde and heterophil/ lymphocyte ratio and significantly decreased
superoxide dismutase (P<0.05). These results showed that supplementation of silymarin in diet under
normal condition, improved the carcass and breast relative weight, but no had effect on bird’s oxidative
status.

Effects of using thyme (Thymus vulgaris L.) and enzyme (Kemine) on performance and blood biochemical parameters of laying Japanese Quails

Pages 139-148

https://doi.org/10.22059/jap.2013.51391

Sohrab Azarfar, Ali Nobakht, Yousef Mehmannavaz

Abstract This experiment was conducted to investigate the effects of using different levels of thyme (Thymus
vulgaris L.) medicinal plant and multi enzyme (Kemine) on performance and blood biochemical
parameters of Japanese quails with 240 laying bird (6 up to 12 weeks) as a 2*2 factorial include two
levels of thyme (0 and 0.75 percent) and 2 levels of Kemine enzyme (0 and 0.05 percent) in four
treatments, 4 replicates and 15 laying quails in each replicate in a completely randomized design.
Interaction between thyme and enzyme did not have any significant effects on egg production
performance. Whereas egg weight in birds who consume thyme, was high (P<0.05) and amounts of blood
uric acid and eggshell thickness in birds who received enzyme and thyme in diets, increased (P<0.05).
The overall results indicated that using enzyme and thyme in Japanese quails diets can be have positive
effects on egg weight and eggshell thickness.