Mohammad Abdoli; Mohammad Bagher Zandi; Taher harkinezhad; Masoud Kahlili
Volume 23, Issue 2 , July 2021, Pages 155-163
Abstract
The aim of this study was to investigate the genetic structure of Iranian native horse breeds to compare genetic diversity and understanding the relationships between the populations using 11 ISAG microsatellite markers. For this reason, 565 samples of the Iranian Equestrian database from different ages ...
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The aim of this study was to investigate the genetic structure of Iranian native horse breeds to compare genetic diversity and understanding the relationships between the populations using 11 ISAG microsatellite markers. For this reason, 565 samples of the Iranian Equestrian database from different ages including the Iranian Arab Asil, Caspian, Darehshouri, Kurdish and Turkmen and 113 samples were used for each breed. The number of observed alleles for each locus was 7 to 19 alleles with an average of 10.81 alleles, and it was 19 for ASB17 and 7 for HTG4 locus with the highest and lowest observed alleles ranking respectively. The average observed heterozygosity from the largest to the lowest rank was, Turkmen (0.68±0.11), Caspian (0.67±0.07), Kurdish (0.66±0.06) Darehshouri (0.65±0.07), and Arab Asil (0.62±0.08). Population structure analysis with UPGMA method showed that Caspian and Kurdish populations were grouped as a unit cluster while the other populations grouped as a separate cluster. These results confirmed this hypothesis that the Caspian and Kurdish populations are close to the Nisa horses. In general, the results of this study indicate that the Iranian native horses have got a high genetic diversity, despite of populations have genetic similarity and the other hand genetic clustering of the populations is consistent with their geographic distances. The result of this study shows that the ISAG microsatellite markers are polymorphic and have more efficiency for assignment genetic diversity and genetic structure analysis of Iranian native horse breeds.
Safora Yousefinejad; Farshid Fatahnia; Mehdi Kazemi Bonchenari
Volume 23, Issue 2 , July 2021, Pages 165-177
Abstract
The aim of this experiment was to investigate the effect of starter soybean oil (SO) and crude protein (CP) level on growth performance, plasma parameters and urinary purine derivatives (UPD) of dairy calves. Forty-eighth 3-old Holstein dairy calves with initial BW of 39.8 ± 2.4 kg were used in ...
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The aim of this experiment was to investigate the effect of starter soybean oil (SO) and crude protein (CP) level on growth performance, plasma parameters and urinary purine derivatives (UPD) of dairy calves. Forty-eighth 3-old Holstein dairy calves with initial BW of 39.8 ± 2.4 kg were used in a completely randomized design with 2 × 2 factorial arrangement. The experimental diets consisted of diet without SO and 19% CP, diet without SO and 22% CP, diet with 3% SO and 19% CP and diet with 3% SO and 22% CP. Feed intake, feces score, skeletal growth indices, plasma parameters, liver enzymes, urinary purine derivatives and microbial protein synthesis were measured. Growth performance, plasma parameters and UPD were not influenced by interaction effect of starter diet SO and CP level. Starter diet intake, average daily gain and feed efficiency were lower in calves fed SO than those fed diets without SO (P < 0.05). The fecal score and wither height of calves fed diets containing SO were higher and lower that those fed diets without SO, respectively (P < 0.05). Plasma concentrations of glucose, beta-hydroxy butyrate and total protein were lower in calves fed SO (P < 0.05). Addition of SO to the diets decreased urinary allantoin and total UPD concentrations (P ≤ 0.05). Microbial protein synthesis tended to be decreased in calves received diets containing SO compared to other groups (P=0.06). Plasma urea nitrogen concentration of calves increased by increasing dietary CP level (P < 0.05). According to this results, growth performance of Holstein dairy calves is not affected by interaction of SO and CP level in starter diet
mohammad ali ahangarani; Mohammadreza Dehghani; abbas ali naserian
Volume 23, Issue 2 , July 2021, Pages 179-189
Abstract
The aim of this research was to investigate the effect of adding fat powder and flaxseed oil in milk on performance, growth, health, blood and rumen parameters of 45 suckling calves (avarage body weight = 38 ± 0.5 kg) in a compeletly randomized design in 10℃ of average temperature from d 3 to ...
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The aim of this research was to investigate the effect of adding fat powder and flaxseed oil in milk on performance, growth, health, blood and rumen parameters of 45 suckling calves (avarage body weight = 38 ± 0.5 kg) in a compeletly randomized design in 10℃ of average temperature from d 3 to 30 of age in individual boxes. Experimental treatments included: 1- basal starter and milk without fat supplement (control) 2- basal starter and milk with fat powder (10 g/liter of milk) 3- basal starter and milk with flaxseed oil (10 g/liter of milk). Daily weight gain from d 3 to 30 and weight at d 30 were higher in flaxseed oil treatment than control treatment (P ≤ 0.05). Calves fed flaxseed oil treatment had higher hip height than control treatment at d 30 (P ≤ 0.05). Blood cholesterol concentration was higher in control treatment than flaxseed oil treatment and blood cortisol concentration was lower in flaxseed oil treatment than control treatment (P ≤ 0.05). The ratio of total protein to albumin in flaxseed oil treatment was lower than control treatment (P ≤ 0.05). Ruminal parameters were not affected by the experimental treatments. The results of this research indicated that using of fat sources including linolenic acid as flaxseed oil in milk have a positive effect on the performance, growth and health during the first month after birth of dairy calves.
mansour Dehghani; Ahmad Afzalzadeh; Mohammad Ali Norouzian
Volume 23, Issue 2 , July 2021, Pages 191-200
Abstract
The present study was conducted to evaluate the effect of urea treatment of wheat straw and sugarcane bagasse on chemical composition, lag time and ruminal degradability parameters of dry matter (DM) and neutral detergent fiber (NDF) by nylon bag technique. The chemical composition and degradability ...
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The present study was conducted to evaluate the effect of urea treatment of wheat straw and sugarcane bagasse on chemical composition, lag time and ruminal degradability parameters of dry matter (DM) and neutral detergent fiber (NDF) by nylon bag technique. The chemical composition and degradability parameters of unprocessed samples and processed samples with five percent urea were determined using three mature rumen-fistulated Holstein dairy cows (with initial body weight of 615±15 kg and three lactation number). Urea treatment decreased DM and NDF degradation lag time of processed wheat straw (P<0.05); however it had no effect on DM and NDF degradation lag time of sugarcane bagasse. The urea treatment resulted to increase in DM and NDF degradability rate of processed wheat straw (P<0.05), but had no effect on DM and NDF degradability rate of sugarcane bagasse. The potentially degradable fraction of DM and NDF of both experimental materials were increased by treatment (P<0.05). The effective degradability of DM and NDF of experimental roughages increased (P<0.05) after urea treatment from 35.69 and 21.69 to 44.04 and 29.12 percent, respectively for wheat straw and from 18.88 and 15.65 to 23.92 and 19.52 percent, respectively for sugarcane bagasse. According to the results of this experiment, urea treatment of roughages-feeds, especially wheat straw improves degradability of DM and NDF.
salman afshar; hamid Amanlou; Davood Zahmatkesh
Volume 23, Issue 2 , July 2021, Pages 201-212
Abstract
The effect of using whole safflower seed and flaxseed in the during transition period on performance, digestibility, production and composition of colostrum and blood parameters of Afshari ewes using three treatments and 27 pregnant ewes with average age of three years, weight of 85 ± 1.28 ...
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The effect of using whole safflower seed and flaxseed in the during transition period on performance, digestibility, production and composition of colostrum and blood parameters of Afshari ewes using three treatments and 27 pregnant ewes with average age of three years, weight of 85 ± 1.28 kg and range 6 weeks before expected lambing in a completely randomized design was investigated. Experimental treatments included; 1- control diet (base), 2- diet containing 8% safflower seed, 3- diet containing 8% flaxseed. The effect of treatments was not significant on functional traits of ewes pre-lambing but their dry matter intake and weight change post lambing were affected by safflower seed and flaxseed treatment (P< 0.05). The experimental treatments were not significant on pre-lambing digestibility coefficients, composition and amount of colostrum and milk as well as lamb performance. The triglyceride, cholesterol and high-density lipoprotein levels in blood plasma of flaxseed-fed ewes were higher than safflower and control treatments in pre-lambing. The results demonstrated that using safflower seed and flaxseed in the period close to lambing did not have negative effects on the performance of gestation ewes but increased their dry matter and improved the health of lactating ewes and lambs by preventing severe weight loss in post lambing.
seyed mohammad reza beheshti; kamran rezayazdi; arya badiei; Mostafa Sadeghi
Volume 23, Issue 2 , July 2021, Pages 213-221
Abstract
The aim of this study was to investigate the effect of different levels of live yeast on ruminal function and health of dairy cows fed diets containing low and high concentrations of non-fiber carbohydrates (NFC). In this experiment, six cannulated dairy cows were randomly assigned in a Latin square ...
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The aim of this study was to investigate the effect of different levels of live yeast on ruminal function and health of dairy cows fed diets containing low and high concentrations of non-fiber carbohydrates (NFC). In this experiment, six cannulated dairy cows were randomly assigned in a Latin square design with six periods (14-days) and six treatments including 1- diet contain low NFC free of live yeast, 2-diet contain low NFC with 5 g/d of live yeast, 3-diet contain low NFC with 10 g/d of live yeast, 4-diet contain high NFC free of live yeast, 5-diet contain high NFC with 5 g/d of live yeast and 6-diet contain high NFC with 10 g/d of live yeast. Dry matter intake, rumination, ruminal parameters and blood metabolites were determined in the different periods. Dry matter intake, rumination activity, nutrient digestibility, mean ruminal pH and temperature were not affected by treatments. General and plasma indicators of animal healthwere also not affected by treatments. Overall, the results of this experiment indicated that dietary supplementation with live yeasthad no significant effect on rumen function and health in cows fed diets containing different concentrations of NFC.
Akbar Yaghobfar; Rezvan Yaghoubfar; Ehsan Zare Banadkoki
Volume 23, Issue 2 , July 2021, Pages 223-233
Abstract
The experiment was conducted to investigate the effects of cell wall carbohydrates with diet supplemented enzyme on the function and expression of glucose transporter genes (SGLT1 and GLUT2), peptide transporter (PepT1) and mucin production (MUC2) in the small intestine of broilers. In this study, 1100 ...
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The experiment was conducted to investigate the effects of cell wall carbohydrates with diet supplemented enzyme on the function and expression of glucose transporter genes (SGLT1 and GLUT2), peptide transporter (PepT1) and mucin production (MUC2) in the small intestine of broilers. In this study, 1100 mixeddayold chickens (male and female), Ross 308 were used based on a completely randomized design with 11 treatments and five replications (20 birds per replication) for 42 days. Experimental diets included control diets, diets containing wheat, barley, wheat bran, rice bran, and hull less barley with and without enzymes, respectively. The results showed that the effect of diets containing wheat, barley and hull less barley with enzyme on the total live weight of broiler chickens at 42 days of age was significantly different (P <0.05).Cell wall carbohydrates of wheat, wheat bran and rice bran in diets increased pancreatic amylase activity (barley 3.02, wheat 5.99 U/mg CP of small intestinal tissue) (P < 0.05). The expression of the studied SGLT1 and MUC2 genes in the experimental diets without enzyme showed a significant increase compared to enzymes supplemented diet (P < 0.05). Also, among the groups of enzyme-supplemented diets, only wheat and rice bran groups were able to increase the expression of SGLT1, MUC2 and GLUT2 genes compared to the control group (P <0.05). In conclusion, supplementation of diets containing cell wall carbohydrates with enzyme affects the expression of glucose transport genes (SGLT1 and GLUT2), peptide transport (PepT1) and mucin production (MUC2) in the small intestine jejunum. This indicates the optimal function of the digestive system of broilers in terms of digestion and absorption of nutrients.
Mohammad Reza Rezvani; Fatemeh Ahmadi
Volume 23, Issue 2 , July 2021, Pages 235-244
Abstract
Effect of red pepper in comparison to commercial growth promoters, Biostrong and Oleobiotec, on growth performance, nutrient digestibility, and other traits of broilers under heat stress conditions using 200 day-old broiler chickens mixed male and female of Arbor Acers strain in a completely randomized ...
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Effect of red pepper in comparison to commercial growth promoters, Biostrong and Oleobiotec, on growth performance, nutrient digestibility, and other traits of broilers under heat stress conditions using 200 day-old broiler chickens mixed male and female of Arbor Acers strain in a completely randomized design with four treatments, five replications and ten chickens per replicate were evaluated. Treatments included a basal diet without additives and a basal diet containing 10, 0.1, 0.15 g/kg red pepper, Biostrong, and Oleobiotec, respectively. At the beginning of third week, the birds were exposed to heat stress for six hours per day. During the 42 days of the experiment, the mean minimum and maximum temperatures were 25°C and 33°C. Feed intake, weight gain, feed conversion ratio, the weight of internal organs, caecal microflora, and precaecal nutrient digestibility were measured for the heat stress period. The use of red pepper improved the feed conversion ratio and daily weight gain in grower and finisher periods and increased protein, lipid, and dry matter precaecal digestibility, the relative weight of bursa of Fabricius, spleen, and gizzard, and decreased the Ecoli population in caecum compared to the control treatment (P≤0.05). Biostrong and Oleobiotec did not affect growth performance, prececal nutrient digestibility, and the E.coli population. Overall, using the red pepper in the diet of broilers compared to commercial growth promoters reduced the adverse effects of heat stress on growth performance, nutrient digestibility, and caecal microflora.
Mohsen Afsharmanesh; Maryam Rezaeipour; Mohamad Salarmoini
Volume 23, Issue 2 , July 2021, Pages 245-254
Abstract
The effect of use of acetic acid, propionic acid, butyric acid, and probiotic on productive performance of laying hens and some quality parameters of produced eggs was evaluated using an experiment with 320 Bowens laying hens, in a 4×2 factorial experiment with four fatty acid levels (no additive, ...
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The effect of use of acetic acid, propionic acid, butyric acid, and probiotic on productive performance of laying hens and some quality parameters of produced eggs was evaluated using an experiment with 320 Bowens laying hens, in a 4×2 factorial experiment with four fatty acid levels (no additive, 0.1 % diet, propionic acid, 0.2 % diet, butyric acid and 0.3 % diet, acetic acid) and two levels of probiotic (0 and 0.01% diet) in a completely randomized design with 8 treatments, 5 replications and 8 birds per replication. The results of production performance showed that the egg weight, feed conversion ratio, production percentage and egg mass parameters, were not affected by the experimental groups. The main effects showed that organic acid supplementation significantly increased feed intake (P<0.05). It was shown that the percentage, diameter and color index of the yolk were not affected but the height of the yolk was improved by supplements (P<0.05). The results of shell quality also showed that specific gravity, shell percentage and shell thickness were not affected by the experimental groups, but diets containing organic acids and probiotic significantly improved shell resistance compared with non-additive group (P<0.05). It could be concluded that organic acid and probiotic supplements with a positive effect on feed consumption, egg weight, egg yolk height and shell resistance, improved production performance and quality of produced eggs.
seyyed mohammadreza salavati; Ahmad Hassanabadi; Mohsen Teimurty; Aliakbar Salari
Volume 23, Issue 2 , July 2021, Pages 255-267
Abstract
The effect of heat processing of wheat grains and enzyme supplementation on its apparent metabolizable energy (AME) and digestibility of protein and dry matter as well as the effect of their use in the diet on the growth performance, jejunum morphology and microbial population of ileum were investigated. ...
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The effect of heat processing of wheat grains and enzyme supplementation on its apparent metabolizable energy (AME) and digestibility of protein and dry matter as well as the effect of their use in the diet on the growth performance, jejunum morphology and microbial population of ileum were investigated. For this purpose, 480 broiler chickens in a 4×2 factorial experiment with three levels of heat processing of wheat at 55, 70 and 85 °C for 2.5 minutes (with one treatment without thermal processing) and Rovabio enzyme (0 and 500 g/ton of diet) was used in a completely randomized design with eight treatments, six replicates of 10 birds each at the age of 25-42 days. Heat processing of wheat at 55 and 85 °C increased its AME compared to unprocessed treatment; So that the AME of processed wheat at 85 °C was 2.49% higher than unprocessed wheat (P<0.05). Enzyme supplementation significantly increased the AME of wheat (P < 0.05). Wheat processing at all three temperatures reduced the height of jejunum villi compared to the unprocessed group (P < 0.01). Also, processing of wheat at 70 and 85 °C increased the population of lactobacilli compared to treatment without processing (P = 0.05). The use of enzyme in the diet reduced the feed intake of the birds and the height of jejunal villi (P<0.05). Overall,, enzyme supplementation and heat processing of wheat improve its AME but have no remarkable effect on the growth performance of broiler chickens.
Kazem Karimi
Volume 23, Issue 2 , July 2021, Pages 269-279
Abstract
In order to investigate the effects of the Tarragon byproducts on the growth performance, carcass traits, cellular and humoral immunoresponse of broilers, one-day- old one hundred sixty-eight Ross 308 chicks were randomly allocated in to four treatment groups in 24 cages (6 replicates per treatment) ...
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In order to investigate the effects of the Tarragon byproducts on the growth performance, carcass traits, cellular and humoral immunoresponse of broilers, one-day- old one hundred sixty-eight Ross 308 chicks were randomly allocated in to four treatment groups in 24 cages (6 replicates per treatment) and 7 chicks per replicate. The treatments were included: 1) basal diet (control), 2) basal diet + 500 μl/lit Hydro alcoholic extract of tarragon in drinking water, 3) basal diet + 1% dietary tarragon powder and 4) basal diet + 1% dietary tarragon powder + 500 μl/lit of tarragon extract in drinking water. Results indicated that at the starter, grower, finisher and total periods of time, the birds that were recived 1% dietary tarragon powder had a better growth performance rather than other birds, so that, feed intake and weight gain were significantly increased (P ≤ 0.05) and feed conversion ratio was improved. In these birds, the relative weights of carcass and bursa of Fabresious was also greater than other birds (P ≤ 0.05). No significant differences were observed in the cellular immunity response (change in foot membrane thickness in response to Phytohemagglutinin injection) and humoral imiunity response (antibody response to Newcastle virus and antibody response to injected SRBC) due to treatments. Based on the results, the use of one percent tarragon powder in the diet of broilers is recommended to improve the growth efficiency and carcass yield.
Vahideh Sabaghi-Darmiyan; Mehran Mehri; Farzad Bagherzadeh Kasmani; Mohammad Rokouei
Volume 23, Issue 2 , July 2021, Pages 281-292
Abstract
The experiment was carried out to investigate the effect of tryptophan, melatonin and dimethylglycine on the growth performance and meat quality of Japanese quails fed diets contaminated with aflatoxin B1 toxin. In this experiment, 680 Japanese quail from 7 to 35 days of age were allotted to 17 ...
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The experiment was carried out to investigate the effect of tryptophan, melatonin and dimethylglycine on the growth performance and meat quality of Japanese quails fed diets contaminated with aflatoxin B1 toxin. In this experiment, 680 Japanese quail from 7 to 35 days of age were allotted to 17 treatments and 4 replicates (10 birds per replication), and trial performed using a central composite design. The performance of birds was calculated on a weekly basis and at the end of the experiment; and the amount of malondialdehyde in meat was determined. Results showed that tryptophan increase weight gain and melatonin had an increased effect on feed intake (P < 0.05). The effect of tryptophan and as well as the interaction of tryptophan and dimethylglycine reduced the amount of malondialdehyde in meat (P < 0.05). The results obtained from this research state that dietary supplementation with tryptophan and methyl group donors have good antioxidant capacity to reduce aflatoxin B1 poisoning on growth performance and malondialdehyde content of meat, and the use of these supplements in the diet of Japanese quails seems desirable.
hamid reza salari; Yousef Jafari Ahangari; Zarbakht Ansari Pirsaraie
Volume 23, Issue 2 , July 2021, Pages 293-302
Abstract
In this research, the effect of feeding coQ10 and omega 3 fatty acids on testicular histology and relative expression of TGF-b4 & StAR genes in Hubbard roosters was investigated. Forty-eight roosters, at fifty weeks age, were randomly divided into four groups and were subjected to the basal diet ...
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In this research, the effect of feeding coQ10 and omega 3 fatty acids on testicular histology and relative expression of TGF-b4 & StAR genes in Hubbard roosters was investigated. Forty-eight roosters, at fifty weeks age, were randomly divided into four groups and were subjected to the basal diet supplemented with omega-3 (30gr), coQ10 (400mg) and omega-3+ coQ10 (30gr+400mg) per day for eight successive weeks. At the end of the trial, all birds were killed and two samples were collected from the same testicle one of which was processed for histology, whereas another was snap-frozen in liquid nitrogen to assess relative gene expression. Results showed that seminiferous tubule diameters of experimental groups were significantly improved compared to the control group (P<0.05). However, leydig cell numbers and sertoli cells were not significantly affected. The relative expression of TGF-b4 genes were significantly decreased in three groups compared to control (P<0.05). Birds fed coQ10 and omega-3 had significantly higher StAR transcript level compared to other groups (P<0.05). Results of the present study showed that addition of a mixture of coQ10 and omega-3 fatty acids to rooster`s feed, lead to improvement in testicular histology and genes expression related to spermatogenesis.
Vahid Vahedi; Babak Naderi; Ciyamak Ghazaie; Azadeh Boustan
Volume 23, Issue 2 , July 2021, Pages 303-312
Abstract
The aim of this study was the comparison of two protocols of estrus synchronization in two natural climates and heat stress condition on reproductive performance and concentration of plasma estrogen and progesterone hormones in Holstein dairy cows. One hundred and ten multiparous Holstein dairy cows ...
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The aim of this study was the comparison of two protocols of estrus synchronization in two natural climates and heat stress condition on reproductive performance and concentration of plasma estrogen and progesterone hormones in Holstein dairy cows. One hundred and ten multiparous Holstein dairy cows (28 kg average daily milk yield and parity one to four calved) were randomly assigned to two protocol groups: 1) Double-Ovsynch (DO; n=60), the cows received GnRH-7d-PGF2α-2d-GnRH and Ovsynch (GnRH-7d- PGF2α-56h-GnRH-16h-AI) was initiated 7 d later; 2) Presynch-Ovsynch (PO; n=50), the cows received PGF2α-14d-PGF2α and Ovsynch was initiated 12 d later. The both of the protocols were done in two seasons of summer and winter. The calving rate in cows that were synchronized at winter season was greater than cows synchronized in summer (36.4 vs 18.2%; P= 0.04). Mean of the number of insemination to conception was lower in cows that synchronized in winter compared to cows synchronized in summer (1.46 vs 1.74; P= 0.02). Mean of measured estrogen and progesterone hormones concentrations was higher in cows that synchronized in winter compared to summer (P < 0.05). Overall, mean of reproductive traits and hormones concentration is higher in cows that synchronized in winter than in summer and the protocol of estrus synchronization had no effect on reproductive performance.