Document Type : Research Paper

Authors

1 , Animal Science Department, College of Agriculture & Natural Resources, University of Gorgan, Iran

2 Animal Science faculty, Gorgan university agricultural sciences & natural resources

3 Sari university of agricultural sciences & natural resources

Abstract

In this research, the effect of feeding coQ10 and omega 3 fatty acids on testicular histology and relative expression of TGF-b4 & StAR genes in Hubbard roosters was investigated. Forty-eight roosters, at fifty weeks age, were randomly divided into four groups and were subjected to the basal diet supplemented with  omega-3 (30gr), coQ10 (400mg) and  omega-3+ coQ10 (30gr+400mg) per day for eight successive weeks. At the end of the trial, all birds were killed and two samples were collected from the same testicle one of which was processed for histology, whereas another was snap-frozen in liquid nitrogen to assess relative gene expression. Results showed that seminiferous tubule diameters of experimental groups were significantly improved compared to the control group (P<0.05). However, leydig cell numbers and sertoli cells were not significantly affected. The relative expression of TGF-b4 genes were significantly decreased in three groups compared to control (P<0.05). Birds fed coQ10 and omega-3 had significantly higher StAR transcript level compared to other groups (P<0.05). Results of the present study showed that addition of a mixture of coQ10 and omega-3 fatty acids to rooster`s feed, lead to improvement in testicular histology and genes expression related to spermatogenesis.

Keywords

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    1. Abdoljabar Ali A, Zhandi A, Tohidi A, Zaghari A and Ansari M (2017). The effect of oral letrozole on plasma gonadotropin concentrations and some testicular histological parameters in broilers at 40 weeks of age. Iranian Journal of Animal Sciences, 2(48): 183-175. (in persian).
    2. Aitken RJ and Roman SD (2008). Antioxidant systems and oxidative stress in the testes. Oxidative Medicine and Cellular Longevity, (1): 15-24.

     

     

    1. Akhlaghi A, Ahangari, YJ, Navidshad B, Pirsaraei, ZA, Zhandi M, Deldar H, Rezvani MR, Dadpasand M, Hashemi SR, Poureslami R and Peebles ED (2014a). Improvements in semen quality, sperm fatty acids, and reproductive performance in aged Cobb 500 breeder roosters fed diets containing dried ginger rhizomes. Poultry Science, 93: 1236-1244.
    2. Bachmid NA, Purba FY, Apada A M S and Sari DK (2019). Morphology and histomorphometric study of 1-to 4-month- old Gaga’chicken’s testes. In IOP Conference Series: Earth and Environmental Science, 343(1): 120-131.    
    3. Ben-Meir A, Burstein E, Borrego-Alvarez A, Chong J, Wong E, avorska T, Naranian T, Chi M, Wang Y, Bentov Y, Alexis J, Meriano J, Sung H K, Gasser D L, Moley K H, Hekimi S, Casper R F and Jurisicova A (2015). Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging. Aging cell, 14: 887-95.
    4. Borghei-Rad SM, Zeinoaldini S, Zhandi M, Moravej H and Ansari M (2017). Feeding rosemary leaves powder ameliorates rooster age-related subfertility. Theriogenology, 101: 35-43.
    5. Castellano CA, Audet I, Bailey JL, Chouinard PY, Laforest JP and Matte JJ (2010). Effect of dietary n-3 fatty acids (fish oils) on boar reproduction and semen quality. Journal of animal science, 88: 2346-2355.
    6. Cerolini S, Kelso RC, Noble BK, Speake F Pizzi and Cavalchini LG (1997a). Relationship between spermatozoan lipid composition and fertility during aging of chickens. Biology Reproduction, 57: 976-980.
    7. Hudson BP (2000). Alternative sources of fat and zinc in broiler breeder diets and their influences on performance. University of Georgia (Athens), Ph.D.Thesis.
    8. Karimi A, Shahneh AZ, Zeynoaldini, S, Kohram H, Pirsaraei Z A and Adibmoradi M (2013). Effect of propylthiouracil on puberty initiation and semen production in broiler breeder roosters. Animal Production Research, 2(2). (in persian).
    9. Kazemizadeh, Zare Shahneh, Zainudini, Yousefi Ar, Heidari Amaleh, Tavakoli Al-Mutawi and Ansari Pirsaraei (2017). The 2effect of curcumin on lipid profiles and some parameters of quality of sperm of broilers. Iranian Journal of Animal Sciences. (in persian).
    10. Kuhnlein U, Nie L, Weigend S, Gavora JS, and Fairfall W (1997). DNA polymorphism in chicken growth gene, response to selection for disease resistance and association with egg production. Journal of Animal Genetics, 20: 145-155.
    11. Letterio JJ and Roberts AB(1998). Regulation of immune responses by TGF- beta. Annual Review Immunology, 16: 137-61.
    12. Luo L, Chen H and Zirkin BR (2001). Leydig cell agin: steroidogenic acute regulatory protin and cholesterol side-chain cleavage enzyme. Journal of Andrology, 22: 149-56.
    13. Modaresi M, Messripour M and Rajaei R(2010). Effect of cinnamon extract on the number of spermatocyte and spermatozoa cells in mice. Iranian Journal of Medicinal and Aromatic Plants, 26 (1): 83-90. (in persian).
    14. Piek E, Heldin Dijke PT (1999). Specificity, diversity, and regulation in TGF-β superfamily signaling. J. FASEB, 13: 2105-2124.
    15. Paulenz H, Taugbøl, Hofmo O and Saarem K (1995). A preliminary study on the effect of dietary supplementation with cod liver oil on the polyunsaturated fatty acid composition of boar semen. Veterinary research communications, 19: 273-284.
    16. Saemi F, Zamiri MJ, Akhlaghi A, Niakousari M, Dadpasand M and Ommati MM (2012). Dietary inclusion of dried tomato pomace improves the seminal characteristics in Iranian native roosters. Poultry Science, 91(9): 2310-2315. (in persian).
    17. Safari A, Shariatmadari R, Sharafi F, Karimi Torshizi and M, Shahverdi MA, (2018). Improvements in semen quality, sperm fatty acids, and reproductive performance in aged Ross breeder roosters fed a diet supplemented with a moderate ratio of n-3: n-6 fatty acids. Poultry Science, 97: 4113-4121.
    18. Sarabia Fragoso J, Pizarro Diaz M, Abad Moreno J C, Casanovas Infesta P, Rodriguez-Bertos A and arger K (2013). Relationships between fertility and some parameters in male broiler breeders (body and testicular weight, histology and immunohistochemistry of testes, spermatogenesis and hormonal levels). Reproduction in Domestic Animals, 48: 345-52.
    19. Sebokova E, Garg ML, Wierzbicki A, Thomson ABR and Clandinin MT (1990). Alteration of the lipid composition of rat testicular plasma membranes by dietary (n-3) fatty acids changes the responsiveness of leydig cells and testosterone synthesis. Journal of Nutrition, 120: 610-8.
    20. Turner TT and Lysiak JJ (2008). Oxidative stress: a common factor in testicular dysfunction. Journal of Andrology, 29: 488-98.
    21. Wu LL, Russell DL, Wong SL, Chen M, Tsai TS, St John JC, Norman RJ, Febbraio MA, Carroll J and Robker RL (2015). Mitochondrial dysfunction in oocytes of obese mothers: transmission to offspring and reversal by pharmacological endoplasmic reticulum stress inhibitors. Development (Cambridge, England), 142: 681-91.
    22. Zanussi HP, Shariatmadari F, Sharafi M and Ahmadi H (2019). Dietary supplementation with flaxseed oil as source of Omega-3 fatty acids improves seminal quality and reproductive performance in aged broiler breeder roosters. Theriogenology, 130: 41-48.