Zahra Aminifard; Ali kiani; Arash Azarfar
Volume 24, Issue 4 , December 2022, , Pages 441-452
Abstract
In this study, nutritional and digestibility value of tomato pomace before (TP) and after oil extraction (DTP) were measured. Lycopene content of TP and its rumen disappearance rate was determined. In a completely randomized design, gas production content of TP and DTP was measured in vitro. Ruminal ...
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In this study, nutritional and digestibility value of tomato pomace before (TP) and after oil extraction (DTP) were measured. Lycopene content of TP and its rumen disappearance rate was determined. In a completely randomized design, gas production content of TP and DTP was measured in vitro. Ruminal disappearance of dry matter and lycopene of TP were determined at 0, 2, 6, 12, 24, 36, 48, 72 and 96 hours of incubation using fistula cows. Ruminal degradability parameters including rapidly degraded fraction (a), slowly degraded fraction (b), fractional rate of degradation (c), potential of degradability (PD) and effective degradability (ED) were estimated. Results showed that lycopene content of TP was 168 mg/kg DM. DTP contained higher crude protein, NDF, and ADF than TP. De-oiled TP showed higher gas production, digestibility of organic matter and short-chain fatty acids and lower N-ammonia as compared to TP. The values for a, b, c, PD and ED for dry matter were 57.1, 0.07, 66.4 and 49.3% and for lycopene were 3.87, 42.1, 0.076, 45.9 and 34.1, respectively. In conclusion, ruminal degradability of lycopene was about 30% meaning that about than 70% of lycopene by-passes the rumen. Moreover, de-oiled tomato pomace has reasonable nutritive values to be used in ruminant nutrition.
Mohammad Ali Abbasi; shokoufe ghazanfari; seyed davood sharifi; Hassan Ahmadi Gavlighi
Volume 23, Issue 3 , September 2021, , Pages 395-408
Abstract
The effect of garden thyme essential oil (TEO) and rapeseed oil (RO) on performance, immune traits and intestinal morphology using 432 one-day-old Ross 308 male broiler as a (3x3) factorial experiment in a completely randomized design including different levels of TEO (0, 300 and 500 ppm) and RO ...
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The effect of garden thyme essential oil (TEO) and rapeseed oil (RO) on performance, immune traits and intestinal morphology using 432 one-day-old Ross 308 male broiler as a (3x3) factorial experiment in a completely randomized design including different levels of TEO (0, 300 and 500 ppm) and RO (1, 3 and 5 percentage) were evaluated with 9 treatments and 4 replicates. Throughout the rearing period, broiler chickens receiving 3% gained more weight than 1% of RO and chickens receiving 300 ppm gained more weight than those without TEO (P˂0.05). The use of 3% RO + 300 ppm TEO increased the thigh percentage compared to 1% RO. At 1 and 5 percentage levels of RO, with increasing levels of TEO in the diet, blood cholesterol and LDL concentrations decreased and blood HDL increased (P<0.01). Levels of 3% and 5% of RO + 300 and 500 ppm of TEO in broiler chicken diets increased lymphocyte percentage and antibody titer against Newcastle disease and decreased heterophil percentage and heterophil to lymphocyte ratio of blood (P<0.05). Broiler chickens receiving diet containing TEO had the highest ratio of villi height to crypt depth (P<0.01). Based on the results, the level of 3% rapeseed oil + 300 ppm thyme essential oil in the diet of broiler chickens can be used to improve performance, immunity and biochemical traits of blood.
sofi darabi; mohammad moeini; Manouchehr Souri; Mohammad Ebrahim Nooriyan Soroor; abdolhamid papzan
Volume 22, Issue 3 , September 2020, , Pages 357-364
Abstract
Present study was designed to investigate the effect of Artichoke (Cynara Scolymus) extract on performance and some blood and anti-oxidantparameters of Sanjabi lambs. Eighteen Sanjabi lambs were randomly allocated to three experimental treatments with six replicates in each individualcage for 75 days. ...
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Present study was designed to investigate the effect of Artichoke (Cynara Scolymus) extract on performance and some blood and anti-oxidantparameters of Sanjabi lambs. Eighteen Sanjabi lambs were randomly allocated to three experimental treatments with six replicates in each individualcage for 75 days. The experimental treatments were: 1) control group including basal diet without Artichoke extract, 2) basal diet plus 5 ml ofartichoke extract, and 3) basal diet plus 10 ml of artichoke extract per kilogram dry matter. The blood samples were collected from jugular vein oflambs before feeding at morning monthly. The concentration of glucose, cholesterol, total protein, urea, albumin, and the activity of glutathioneperoxidase, superoxide dismutase, alkaline phosphatase, alanine aminotransferase enzymes and malondialdehyde were measured. The results of thisexperiment showed that artichoke extract had no significant effect on lambs' performance and growth parameters, but significantly decreased bloodconcentration of cholesterol, triglyceride and malondialdehyde index at the level of 10 ml extract per Kg/DM of diet. The urea and cholesterolconcentrations affected by time and at days 40 of experiment, the amount of this parameters was lower than other times. In general, it can beconcluded that artichoke extract at the level of 10 ml extract had no significant effect on average daily gain and growth performance of lambs butimproved some blood parameters.
Volume 19, Issue 2 , August 2017, , Pages 493-506
Abstract
The goal of this paper was to study the effect of ginger extracts and vitamin E on the performance of ovine Spermatogonial Stem Cells (SSCs). For this purpose, different concentrations of hydro and hydroethanolic extracts of ginger and vitamin E on viability, colony formation and expression of inhibiting ...
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The goal of this paper was to study the effect of ginger extracts and vitamin E on the performance of ovine Spermatogonial Stem Cells (SSCs). For this purpose, different concentrations of hydro and hydroethanolic extracts of ginger and vitamin E on viability, colony formation and expression of inhibiting (bcl2ll and bcl2)and inducing (bax) apoptosisgenes were studied. Spermatogonial Stem Cells (SSCs) were extracted from lamb testes with slaughterhouse origin using two steps enzymatic digestion method and enrichment by differential plating method. The characterization of SSCs was carried by alkaline phosphatase staining and expression of c-kit and oct-4 genes. Results have shown that the viability of SSCs was decreased significantly by using more than 800 µg/mL of hydroethanolic extract of ginger, in comparision with control group (P<0.05), while 800 µg/mL of hydro extract of ginger has been resulted to be the most viable cells, and increasing the vitamin E concentration (upto 100 µg/mL) resulted to be the most survival of SSCs. Expression of bax in effective concentration of hydro extract of ginger (150 µg/mL) and vitamin E (50 µg/mL) was decreased, but hydroethanolic extract of ginger (150 µg/mL) and higher concentration of hydro extract of ginger (more than 800 µg/mL) increased the expression od bax. Based on the results of current study, 150 µg/mL of aqueous extract of ginger or 50 µg/mL of vitamin E can be used to improve ovine SSC culture system.
Arash Kheradmand
Volume 18, Issue 4 , December 2016, , Pages 841-852
Abstract
This study was carried out to evaluate the effects of α-tocopherol, BSA and vitamin C on different caprine sperm parameters following incubation at 5 °C for 48 h. The treatments were as follows: 5 and 10 IU/ml of α-tocopherol, 4 and 8 mg/ml of BSA, as well as 3 and 6 mg/ml of vitamin ...
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This study was carried out to evaluate the effects of α-tocopherol, BSA and vitamin C on different caprine sperm parameters following incubation at 5 °C for 48 h. The treatments were as follows: 5 and 10 IU/ml of α-tocopherol, 4 and 8 mg/ml of BSA, as well as 3 and 6 mg/ml of vitamin C, and control group without any antioxidant. Total sperm motility, progressive motility and HOS test were assessed at h 0, 24 and 48. Using 4 mg of BSA and 5 IU of vitamin E increased sperm motility and plasma membrane integrity compared to other antioxidants (P<0.05). Although, higher concentrations of these antioxidants relatively enhanced sperm quality parameters, however, it was still greater than the control group (P<0.05). Different concentrations of vitamin C did not affect sperm quality parameters. According to the results of the present study, addition of BSA and vitamin E to buck semen improved total and progressive sperm motility as well as plasma membrane integrity during 48 h after incubation at 5 °C.
Hanie Shafigh; Malek Shakeri; Saeed Zeinoaldini; Hamid Kohram; Mehdi Zhandi; Morteza Moghbeli
Volume 18, Issue 3 , October 2016, , Pages 615-624
Abstract
The aim of the present research was to study the effect of different concentrations of rosemary (Rosmarinus officinalis L.) extract, (0, 10, 12.5, 16.6, 25 and 50) mg/L added to semen extender, on sperm qualitative and quantitative parameters after freezing-thawing process of rooster sperm. 10 Ross strain ...
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The aim of the present research was to study the effect of different concentrations of rosemary (Rosmarinus officinalis L.) extract, (0, 10, 12.5, 16.6, 25 and 50) mg/L added to semen extender, on sperm qualitative and quantitative parameters after freezing-thawing process of rooster sperm. 10 Ross strain rooster were used. Semen samples were collected, 2 times a week. After adding the samples of semen based on the lecithin extender, they were placed and preserved at 5 ° C. The treatments of 10 and 12.5 mg/L of Rosemary significantly improved the mobility, in addition, treatments of 10, 12.5 and 16.6 mg/L improved progressive motility and viability before freezing (p≤ 0.05). The lowest and highest motility rates were respectively found in treatments of 0 and 10 mg/L of essential oil of rosemary (p≤ 0.05). Functional integrity of the sperm plasma membrane increased with treatment of 10 mg/L of essential oil of rosemary in comparison to other groups, except 12.5 mg/L. Also treatments of 10, 12.5 and 16.6 mg/L significantly decreased the apoptosis. According to these results, it seems that adding the levels of 10 and 12.5 mg/L rosemary extract’ in semen, based on lecithin extender can improve rooster’s sperm quality after thawing.
Seyed Moones ـalali-Kheli Kohi; Mehrdad Mohammadi; Mohammad Roostaei-Ali Mehr
Volume 18, Issue 2 , June 2016, , Pages 377-385
Abstract
Antioxidant effect of olive leaf extract (OLE) was studied on motility, viability, plasma membrane integrity of spermatozoa and malondialdehyde production in 12 Ross 308 roosters at their 30 weeks of age. Semen samples were collected by abdominal massage in 5 times. In each session after the initial ...
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Antioxidant effect of olive leaf extract (OLE) was studied on motility, viability, plasma membrane integrity of spermatozoa and malondialdehyde production in 12 Ross 308 roosters at their 30 weeks of age. Semen samples were collected by abdominal massage in 5 times. In each session after the initial sperm assessment, collected samples were pooled and diluted with Sexton extender.Samples were split into five parts and the concentrations of 0 (control), 50, 100, 150 and 200 µg/mL OLE were added to each part, then,the samples were incubated for 72 hours at 4 degree Celsius. Progressive motility, viability and plasma membrane integrity were evaluated at 0, 24, 48 and 72 hours of storage and production of malondialdehyde were analyzed after 48 hours of storage. Adding 100 µg/mL OLE to semen reduced malondialdehyde production (P<0.05). Using 100 µg/mL of olive leaf extract, progressive motility, viability and plasma membrane integrity of sperm were higher compared to the control group after 48 and 72 hours of storage (P<0.05). Using 200 µg/mL of olive leaf extract, progressive motility, viability and plasma membrane integrity of sperm were lower compared to the control group after 72 hours of storage (P<0.05). Based on the results of this research, adding 100 µg/mL OLE to diluent is recommended for rooster sperm storage at4 degree Celsius.