Document Type : Research Paper
Authors
1 Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran-Iran
2 Department of Animal and Poultry Sciences, College of Aburaihan, University of Tehran, Pakdasht, Tehran-Iran
3 Department of Food Science and Technology, Faculty of Agriculture, Tarbiat Modares University
Abstract
The effect of garden thyme essential oil (TEO) and rapeseed oil (RO) on performance, immune traits and intestinal morphology using 432 one-day-old Ross 308 male broiler as a (3x3) factorial experiment in a completely randomized design including different levels of TEO (0, 300 and 500 ppm) and RO (1, 3 and 5 percentage) were evaluated with 9 treatments and 4 replicates. Throughout the rearing period, broiler chickens receiving 3% gained more weight than 1% of RO and chickens receiving 300 ppm gained more weight than those without TEO (P˂0.05). The use of 3% RO + 300 ppm TEO increased the thigh percentage compared to 1% RO. At 1 and 5 percentage levels of RO, with increasing levels of TEO in the diet, blood cholesterol and LDL concentrations decreased and blood HDL increased (P<0.01). Levels of 3% and 5% of RO + 300 and 500 ppm of TEO in broiler chicken diets increased lymphocyte percentage and antibody titer against Newcastle disease and decreased heterophil percentage and heterophil to lymphocyte ratio of blood (P<0.05). Broiler chickens receiving diet containing TEO had the highest ratio of villi height to crypt depth (P<0.01). Based on the results, the level of 3% rapeseed oil + 300 ppm thyme essential oil in the diet of broiler chickens can be used to improve performance, immunity and biochemical traits of blood.
Keywords
- Abbasi MA, Ghazanfari S, Sharifi SD and Ahmadi Gavlighi H (2019) The Effect of rosemary, thymus and satureja essential oils, vitamin E and vegetable oils on immune system and intestinal microflora of broiler chicken. Journal of Veterinary Research, 74(2): 153-166. In Persian.
- Abbasi MA, Ghazanfari S, Sharifi SD and Ahmadi Gavlighi H (2019) Influence of dietary plant fats and antioxidant supplementations on performance, apparent metabolizable energy and protein digestibility, lipid oxidation and fatty acid composition of meat in broiler chicken. Veterinary Medicine and Science, 00: 1-15.
- Abbasi MA, Mahdavi AH, Samie AH and Jahanian R (2014) Effects of different levels of dietary crude protein and threonine on performance, humoral immune responses and intestinal morphology of broiler chicks. Brazilian Journal of Poultry Science, 16: 35-44.
- Al-Kassie GA (2009) Influence of two plant extracts derived from thyme and cinnamon on broiler performance. Pakistan Veterinary Journal, 29: 169-173.
- Al-Kassie GAM (2010) The role of peppermint (Mentha piperita) on performance in broiler diets. Agriculture and Biology Journal of North America, 15: 1009-1013.
- Carrillo PC, Cavia CMDM and Alonso de la Torre S (2012) Role of oleic acid in immune system; mechanism of action; a review. Nutricion Hospitalaria, 27(4): 978-990.
- Cross DE, Acamovic T, Deans SG and McDevitt RM (2002) The effects of dietary inclusion of herbs and their volatile oils on the performance of growing chickens. British Poultry Science, 43: 33- 35.
- Giannenas I, Florou-paneri P, Papazahariadou M, Botsoglou N, Christaki E and Spaisn AB (2003) Effect of dietary supplementation with oregano essential oil on performance of broilers after experimental infection with Eimeria tenella. Archives in Animal Nutrition, 57: 99-106.
- Goldstein JL and Brown MS (1990) Regulation of the mevalonate pathway. Nature, 343: 425-430.
- Hernandez F, Madrir J and Garcia V (2004) Influence of two plant extracts on broiler performance, digestibility and digestive organ size. Poultry Science, 83(2): 169-174.
- Iji PA, Saki AA and Tivey DR (2001) Intestinal development and body growth of broiler chicks on diets supplemented with non-starch polysaccharides. Animal Feed Science and Technology. 89: 175-188.
- Khattak F, Ronchi A, Castelli P and Sparks N (2014) Effects of natural blend of essential oil on growth performance, blood biochemistry, cecal morphology, and carcass quality of broiler chickens. Poultry Science, 93(1): 132-137.
- Klasing KC and Korver DR (1999) The role of diet in modulating the immune response of broilers: the example of PUFAs. Recent Advances in Animal Nutrition in Australia, 12: 15-20.
- Leeson S and Summers JD (2008) Commercial poultry nutrition, 3rd Nottingham University Press, Nottingham, England.
- Mahdavi AH, Rahmani HR, Nili N, Samie AH, Soleimanian-Zad S and Jahanian R (2010) Effects of dietary egg yolk antibody powder on growth performance, intestinal Escherichia coli colonization, and immunocompetence of challenged broiler chicks. Poultry Science, 89: 484-494.
- Mohammadi Z, Ghazanfari S and Adibmoradi M (2013) Effects of clove essential oil on growth performance, carcass characteristics and immune system in broiler chicken. Veterinary Journal (Pajouhesh & Sazandegi), 102: 67-76. In Persian.
- Nobakht A (2013) The effects of thyme, nettle and alfaalfa on performance, carcass details, blood parameters and immune response on boiler chicks. Animal science khowledge and research Journal, 10: 59-72 (In Persian).
- Sahin K, Onderci M, Sahin N, Gursu MF and Kucuk O (2003) Dietary vitamin C and folic acid upplementation ameliorates the detrimental effects of heat stress in Japanese quail. The Journal of Nutrition, 133: 1882-1886.
- Sanz M, Lopez-Bote CJ, Menoyo D and Bautista JM (2000) Abdominal fat deposition and fatty acid synthesis are lower and β-oxidation is higher in broiler chickens fed diets containing unsaturated rather than saturated fat. The Journal of Nutrition, 130: 3034-3037.
- SAS (2005) SAS User′s guideStatistics. Version 8. SAS Inst. Inc., Cary, NC, USA.
- Spernakova D, Mate D, Rozanska H and kovac G (2007) Effects of dietary rosemary extract and alfa-tocopherol on the performance of chickens, meat quality, and lipid oxidation in meat storaged under chilling conditions. Bulletin Veterinary Institute in Pulawy, 51: 585-589.
- Stanaćev VŽ, Milošević N, Pavlovski Z, Milić D, Vukić Vranješ M, Puvača N and Stanaćev VS (2014) Effects of dietary soybean, flaxseed and rapeseed oil addition on broilers meat quality. Biotechnology in Animal Husbandry, 30(4): 677-685.
- Windisch W, Schedle K, Plitzner C and Kroismary A (2008) Use of phytogenic products as feed additives for swine and poultry. Journal of Animal Science, 86: 140-148.
- Zeinali S, Ghazanfari S and Ebrahimi MA (2017) Mucin2 gene expression in the chicken intestinal goblet cells are affected by dietary essential oils. Bulgarian Journal of Agricultural Science, 23(1): 134-141.
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