Document Type : Research Paper

Authors

• Seyed Moones ـalali-Kheli Kohi ¹
• Mehrdad Mohammadi ²
• Mohammad Roostaei-Ali Mehr ²

¹ Former Graduate Student, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht - Iran

² Associate Professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht - Iran

Abstract

Antioxidant effect of olive leaf extract (OLE) was studied on motility, viability, plasma membrane integrity of spermatozoa and malondialdehyde production in 12 Ross 308 roosters at their 30 weeks of age. Semen samples were collected by abdominal massage in 5 times. In each session after the initial sperm assessment, collected samples were pooled and diluted with Sexton extender.Samples were split into five parts and the concentrations of 0 (control), 50, 100, 150 and 200 µg/mL OLE were added to each part, then,the samples were incubated for 72 hours at 4 degree Celsius. Progressive motility, viability and plasma membrane integrity were evaluated at 0, 24, 48 and 72 hours of storage and production of malondialdehyde were analyzed after 48 hours of storage. Adding 100 µg/mL OLE to semen reduced malondialdehyde production (P<0.05). Using 100 µg/mL of olive leaf extract, progressive motility, viability and plasma membrane integrity of sperm were higher  compared to the control group after 48 and 72 hours of storage (P<0.05). Using  200 µg/mL of olive leaf extract, progressive motility, viability and plasma membrane integrity of sperm were lower  compared to the control group after 72 hours of storage (P<0.05). Based on the results of this research, adding 100 µg/mL OLE to diluent is recommended for rooster sperm storage at4 degree Celsius.

Keywords

• Antioxidant
• Malondialdehyde
• olive
• Rooster
• Spermatozoa

20.1001.1.20096776.1395.18.2.16.7