Document Type : Research Paper

Authors

1 Associate Professor, Animal Science Research Department, Ilam Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Ilam, Iran

2 Assistant Professor, Department of Animal Science Research, Ilam Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization, Ilam, Iran

3 , Department of animal science, Ilam Branch, Islamic Azad University, Ilam, Iran

4 Animal Science Research Department, Ilam Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Ilam, Iran

Abstract

The aim of this study was to investigate the effects of olive leaf powder feeding in different breeding periods on immunity, intestinal bacterial population and blood biochemical parameters of Arian broiler chickens. A total of 336 one-day-old male broiler (Arian strain 386) were studied in a completely randomized design with six treatments for 42 days. The experimental treatments included: control diet (without olive leaves) and the other groups were fed a diet containing 1% olive leaves: from one to14 days, one to 21 days, one to 28 days, one to 35 days and one to days. Body weight gain, feed intake and feed conversion ratio were similar in all groups, but the mortality rate in one to 42 days treatment was lower than other treatments (P <0.01). The amount of low-density lipoprotein-cholesterol in one to 35 days and one to 42 days treatments was lower than other treatments (P <0.05). The alanine aminotransferase activity in the control treatment was higher and in the one to42 days treatment was lower than other treatments (P <0.05). Higher population of Lactobacillus were observed for treatments of one to 28 days, one to 35 days and one to 42 days in jejunum (P <0.01) and one to 21 days, one to 28 days, one to 35 days and one to 42 days in ileum. (P <0.05). The results of this experiment showed that feeding 10 gr/kg of dry olive leaves during the 42 days of the breeding period of broilers led to a reduction in mortality without any negative effect on performance and immunity.

Keywords

1. Agah MJ, Nassiri-Moghadam H, Golian A, 
Raji AR, Farhosh R and Zarban A (2014) 
Effect of dietary Olive leaves (Olea europaea 
L.) extracts and sesame (Sesamum indicumL.) 
oil on intestinal morphology and some blood 
parameters in broiler chickens. Iranian 
Veterinary Journal 11(2): 33-43. (In Persian) 
2. Amouoghli-Tabrizi B and Mohajeri D (2010) 
Protective effect of edible turmeric (Curcuma 
longa Linn.) powder on earlyhepatic injury in 
diabetic rats. Feyz, Journal of Kashan 
University of Medical Sciences 14(3): 190-
199. (In Persian) 
3. AOAC (1990) Official Methods of Analysis. 
15th Ed. Association of Official Analytical 
Chemists, Arlington, VA, USA. 
4. Barreto MSR, Menten JFM, Racanicci AMC, 
Pereira PWZ and Rizzo P (2008) Plant extracts 
used as growth promoters in broilers. Brazilian 
Journal of Poultry Science 10: 109-115. 
5. Caturla N, Perez-Fons L, Estepa A and Micol 
V (2005) Differential effects of oleuropein, a 
biophenol from Olea europaea, on anionic and 
zwiterionic phospholipid model membranes. 
Chemistry and Physics of lipids 137: 2-17. 
6. Domenico T, Francesco C, Maria-Grazia S, 
Vincenza V, Mariateresa C and Claudia D 
(2005) Mechanisms of antibacterial action of 
three monoterpenes. Antimicrobial Agents and 
Chemotherapy 49: 2474- 2478. 
7. Erener G, Ocak N, Ozturk E, Cankaya S and 
Ozkanca R (2020) Evaluation of olive leaf 
extract as a growth promoter on the 
performance, blood biochemical parameters, 
and caecal microflora of broiler chickens. 
Brazilian Journal of Animal Science 49: 1-11. 
8. Haddadin MSY (2010) Effect of olive leaf 
extracts on the growth and metabolism of two 
probiotic bacteria of intestinal origin. Pakistan 
Journal of Nutrition 9(8): 787-793. 
9. Heckert RA, Estevez I, Russek-Cohen E and 
Pettit-Riley R (2002) Effects of density and 
perch availability on the immune status of 
broilers. Poultry Science 81: 451-457. 
10. Huang PL and Huang SL (2010) Oleuropein and 
related compounds reduce atherosclerosis. The 
Open Conference Proceedings Journal 1: 81-86. 
11. Jabalbarezi-Hukerdi Y, Fathi MH, Rashidi L and 
Ganjkhanlou M (2018) The study of 
physicochemical properties and nutrient 
composition of mari olive leaf cultivated in iran. nutrition and food science research 5:39-46.12. Lee KW, Everts H and Beynen AC (2004) 
Essential oils in broiler nutrition. Poultry 
Science 3: 738-752. 
13. Lockyer S, Rowland I, Spencer JPE, Yaqoob P 
and Stonehouse W (2017) Impact of 
phenolic-rich olive leaf extract on blood pressure,
plasma lipids and inflammatory markers: a 
randomized controlled trial. European Journal of 
Nutrition 56(4): 1421-1432. 
14. Monavari Khiyavi S (2017) Effect of Olive 
Leaf Powder on Production Traits and Immune 
System of Broilers. University of Mohaghegh 
Ardabili. Master’s Dissertation. (In Persian). 
15. Otero DM, Oliveira FM, Lorini A, Antunes BF 
and Oliveira RM (2020) Oleuropein: Methods 
for extraction, purifying and applying. Short 
communication. Revista Ceres 67 (4). 
16. Parvardeh S, Niapoor M and Hosseinzadeh H 
(2002) Hepatoprotective activity of Pistacia
Vera L. gum extract in rats. Journal of 
Medicinal Plants 4(4): 27-34. (In Persian) 
17. Peterson AL, Qureshi MA, Ferket PR and 
Fuller JC (1999) Enhancement of cellular and 
humoral immunity in young broilers by the 
dietary supplementation of β-hydroxy- βmethylbutyrate. Immunopharmacology and 
Immunotoxicology 21(2): 307-330. 
18. Qureshi MA and Havenstein GB (1994) A 
comparison of the immune performance of a 
1991 commercial broiler with a 1957 random 
bred strain when fed typical 1957 and 1991 
broiler diets. Poultry Science 73: 1805-1812. 
19. Sabetan-Shiraze AA, Hassanabadi A, Agah MJ 
and Nasiri-Moghaddam H (2016) Eeffect of 
dietary inclusion of olive leaf (Olea europaea 
L.) powder on performance, small intestine 
morphology and nutrient digestibility in broiler 
chickens. Journal of Animal Production 19(2): 
371-387. (In Persian) 
20. Schalm OW, Jain NC and Carroll EJ (1975) 
Veterinary haematology. 3th Ed. Lea and 
Freiberg pablication, Philadelphia. 
21. Shafey TM, Almufarij SI and Albatshan HA 
(2013) Effect of feeding olive leaves on the 
performance, intestinal and carcass 
characteristics of broiler chickens. International 
Journal of Agriculture and Biology 15: 585-589. 
22. Sung JH, Choi SJ, Lee SW, Park KH and Moon 
TW (2004) Isoflavones found in Korean soybean 
paste as 3-hydroxy-3-methylglutaryl coenzyme: 
A reductase inhibitors. Bioscience, 
Biotechnology, and Biochemistry 68: 1051-1058. 
23. Talhaoui N, Taamalli A, Gómez-Caravaca 
AM, Fernández-Gutiérrez A and SeguraCarretero A (2015) Phenolic compounds in 
olive leaves: Analytical determination, biotic 
and abiotic influence, and health benefits. 
Food Research International 77: 92-108. 
24. Varmaghany S, Bastami F and Akbari Gharaei M
(2017) The effect of different periods the use of 
olive leaf in diet on growth performance, 
hematology and ascites syndrome in broiler 
chickens. Journal of Animal Production 19(1): 
175-187. (In Persian) 
25. Varmaghany S, Rahimi S, Karimi-Torshizi MA, 
Lotfollahian H and Hassanzadeh M (2013) Effect 
of olive leaves on ascites incidence, 
hematological parameters and growth 
performance in broilers reared under normal and 
cold temperature conditions. Animal Feed 
Science and Technology 185: 60-69.