Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X

The effect of Asparagus officinalis extract on Arabi ram semen quality during storage in liquid condition at 4 ◦C

Document Type : Research Paper

Authors

Abuzar gholampour 1
Khalil Mirzadeh 2
Saleh TabatabaeiVakili 3

1 M.Sc. Student of Animal Physiology, Department of Animal Science, Ramin Agriculture and Natural Resources University of Khuzestan

2 Department of Animal Science, Agricultural Sciences and Natural Resources University of Khuzestan

3 Associate Professor, Department of Animal Science, Ramin Agriculture and Natural Resources University of Khuzestan

https://doi.org/10.22059/jap.2021.325561.623624
Abstract
The study aimed to investigate the effect of asparagus extract on quality semen of Arabian rams at different storage times at a temperature of 4 degrees Celsius. Sperm collection was performed from 10 Arab rams with an average age of 2.5 years per week (once a week) for six weeks. After adding 0, 1.5, 3 and 4.5% asparagus extract to the semen diluent, sperm quality parameters were evaluated at 0, 24, 48, and 72 hours after storage in liquid condition. At zero hour, sperm viability in diluent had 3% asparagus extract was higher than diluent containing 0 and 4.5% extract (P<0.05). After 24 hours of semen storage, the parameters of total motility, progressive motility, plasma membrane integrity and viability were higher in sperm diluent containing 1.5 and 3% of the extract (P<0.05). Qualitative parameters of sperm in 48 hours after sperm collection and storage in diluent contain 3% of extract had significant and better performance (P<0.05). The highest rate of plasma membrane integrity, progressive motility and total sperm motility was observed in diluent containing 3% extract in 72 hours after sperm storage (P<0.05). According to the results of this study, the use of asparagus extract in the diluent at the rate of 3% improves the sperm quality of Arabian rams after cooling conditions.

Keywords

Arabic ram
Asparagus
Motility
Sperm
Storage
  1. Amaro-Lopez M, Zurera-Cosano G, Moreno-Rojas, R (1998) Trends and nutritional significance of mineral content in fresh white asparagus spears. Journal Food Science 49: 353-363.
  2. Becerra VAB (2017) Effect of the addition of Aniba Canelilla, Blueberry and Green Tea Polyphenol on the in vitro viability of buffalo sperm (Bubalus Bubalis) cooled at 5º C.
  3. Colás C, Cebrián‐Pérez JA and Muiño‐Blanco T (2010). Caffeine induces ram sperm hyperactivation independent of cAMP‐dependent protein kinase. International journal of andrology, 33(1): e187-e197.
  4. De Castro LS, De Assis PM, Siqueira AF, Hamilton TR, Mendes CM and Losano JD, Assumpção ME (2016) Sperm oxidative stress is detrimental to embryo development: a dose-dependent study model and a new and more sensitive oxidative status evaluation. Oxidative Medicine and Cellular Longevity, 2016.
  5. Fan R, Yuan F, Wang N, Gao Y and Huang Y (2015) Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L. Journal of food science and technology, 52(5): 2690-2700.
  6. Ferramosca A, Lorenzetti S, Di Giacomo M, Lunetti P, Murrieri F, Capobianco L and Zara V (2021) Modulation of human sperm mitochondrial respiration efficiency by plant polyphenols. Antioxidants, 10(2): 217.
  7. Forouzanfar M, Fazilati M, Hosseini SM, Moulavi F, Hajian M, Salehi SA and Nasr-Esfahani MH (2007) Investigation of different glycerol and egg yolk concentration on freezing Bakhtiari ram semen. Journal of Iranian Anatomical Sciences, 5: 17-25
  8. Gadea J, Gumbao D, Gómez-Giménez B and Gardón JC (2013) Supplementation of the thawing medium with reduced glutathione improves function of frozen-thawed goat spermatozoa. Reproductive biology, 13(1): 24-33
  9. Hammerstedt RH and Graham JK (1992) Cryopreservation of poultry sperm: the enigma of glycerol. Cryobiology, 29(1): 26-38.
  10. Kamboj P, Aggarwal M, Puri S and Singla SK (2011) Effect of aqueous extract of Tribulus terrestris on oxalate-induced oxidative stress in rats. Indian journal of nephrology, 21(3): 154.
  11. Karimi A, Besharati M and Nemati Z (2018) Protective effects of fennel (Foeniculum vulgare) extract onfrozen-thowed sperm of Ghezel ram. Journal of Animal Environment, 10(4): 83-90. (In Persian).
  12. Khaleghi S, Bakhtiari M, Asadmobini A and Esmaeili F (2017) Tribulus terrestris extract improves human sperm parameters in vitro. Journal of evidence-based complementary and alternative medicine, 22(3): 407-412. (In Persian).
  13. Lee EJ, Yoo KS and Patil BS (2010) Development of a rapid HPLC‐UV method for simultaneous quantification of protodioscin and rutin in white and green asparagus spears. Journal of food science, 75(9): 703-709.
  14. Naghdi Badi H, Qavami N, Mehrafarin A and Qaderi A (2011) A Review on Garden Asparagus (Asparagus officinalis ) as a Medicinal and Multipurpose Plant. Journal Medical Plants. 10 (39): 1-13.
  15. Nantia EA, Moundipa PF, Monsees TK and Carreau S (2009) Medicinal plants as potential male anti-infertility agents: a review. Basic and Clinical Andrology, 19(3): 148-158.
  16. Pall E, Cenariu M, Groza I, Ciupe S, Sinteonean L and Bogdan L (2018) Effect of ascorbid acid and L-glutamine on ram sperm parameters. Lucrari Stiintifice-Universitatea de Stiinte Agricole a Banatului Timisoara, Medicina Veterinara, 51(2): 85-91.
  17. Pokorný J (2007) Are natural antioxidants better–and safer–than synthetic antioxidants. European Journal of Lipid Science and Technology, 109(6): 629-642.
  18. Ros-Santaella JL and Pintus E (2021) Plant Extracts as Alternative Additives for Sperm Preservation. Antioxidants, 10(5): 772.
  19. Schwarzbach A, Schreiner M and Knorr D (2006) Effect of cultivars and deep freeze storage on saponin content of white asparagus spears (Asparagus officinalis). European Food Research and Technology, 222(1): 32-35
  20. Shi LG, Yang RJ, Yue WB, Xun WJ, Zhang CX, Ren YS, Shi L, Lei FL. 2010. Effect of elemental nano-selenium on semen quality, glutathione peroxidase activity, and testis ultrastructure in male Boer goats. Animal Reproduction Science, 118(2-4): 248-254.
  21. Shi, L, Jin T, Hu Y, Ma Z and Niu H Ren Y )2020) Effects of reduced glutathione on ram sperm parameters, antioxidant status, mitochondrial activity and the abundance of hexose transporters during liquid storage at 5℃. Small Ruminant Research, 106139.
  22. Talevi R, Barbato V, Fiorentino I, Braun S, Longobardi S and Gualtieri R (2013) Protective effects of in vitro treatment with zinc, d-aspartate and coenzyme q10 on human sperm motility, lipid peroxidation and DNA fragmentation. Reproductive Biology and Endocrinology, 11(1): 1-10.
  23. Vahedi V, Hedayat Evrigh N, Behroozlak M and Dirandeh E (2018) Antioxidant effects of thyme (Thymus vulgaris) extract on ram sperm quality during cryopreservation. Iranian Journal of Applied Animal Science, 8(2): 263-269. (In Persian).
  24. Zhu XL, Li CX, Zhang L and Zhu YD (2020) Research advance in bioactive constituents and major biological activities of Asparagus officinalis L. journal food science, 9(1): 74-81.

 

Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X
Volume 23, Issue 4
Winter 2022
Pages 583-592

Home

Guide for Authors

Submit Manuscript

Reviewers

Contact Us