Document Type : Research Paper

Authors

1 MSc Graduated Student/Sari Agricultural Sciences and Natural Resources University

2 Scientific staff/Sari Agricultural Sciences and Natural Resources University

Abstract

Effect of Basil powder and extract on yield and carcass characteristics, relative expression of heat shock protein gene (HSP70) and some blood parameters of broiler chickens under heat stress using 160 Ross 308 one-day-old broiler chicks in a completely randomized design with 5 treatments and 4 replications (8 chickens per replication), was evaluated for 42 days. Treatments included: 1- Basic diet (control), 2- Basic diet+ Vitamin C (250 mg per kg of grain), 3- Basic diet + 25 mg of Basil powder (per kg of grain), 4- Basic diet +50 mg of Basil powder (per kg of grain), 5- The use of Basil extract (1 ml/liter water). Feed intake, body weight gain, feed conversion ratio, carcass characteristics, blood parameters and relative expression of HSP70 gene in blood were measured. The effects of treatments on blood parameters and carcass characteristics were not significant. Relative expression of the HSP70 gene in the blood was significantly reduced in all treatments compared to the control (P<0.05). Based on the results, basil powder and extract reduce the negative effects of heat stress in broilers, so their use in broiler diets is recommended to prevent heat stress.

Keywords

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    1. Amiri M, Ghasemi HA, Hajkhodadadi I and Khaltabadi Farahani AH (2019) Efficacy of guanidinoacetic acid at different dietary crude protein levels on growth performance, stress indicators, antioxidant status, and intestinal morphology in broiler chickens subjected to cyclic heat stress. Animal Feed Science and Technology, 254: 114208.
    2. Asadi N, Husseini SD, Tohidian MT, Abdali N, Mimandipoure A, Rafieian-Kopaei M and Bahmani M (2017) Performance of broilers supplemented with peppermint (Mentha piperita) powder. Journal of Evidence-Based Integrative Medicine, 22: 703-706.

     

     

    1. Asasi k, Nilly H (2002) Breeding domestic birds in very hot climates. First edition, Shiraz University Press. Pages 43-74. (in Persian)
    2. Attia YA, Al-Harthi MA, El-Shafey AS, Rehab YA and Kim WK (2017) Enhancing tolerance of broiler chickens to heat stress by supplementation with vitamin E, vitamin C and/or probiotics. Annals of Animal Science, 17: 1155-1169.
    3. Borges SA, Fischer Da, Silva AV, Majorka A, Hooge DM and Cummings KR (2004) Physiological responses of broiler chickens to heat stress and dietary electrolyte balance (sodium plus potassium minus chloride, miliequivalents per kilogram). Poultry Science, 83: 1551-1558.
    4. Cheng JH (2016) Lipid Oxidation in Meat. Journal of Nutrition and Food Sciences, 6(6): 492.
    5. Febbraio MA and Koukoulas I (2000) HSP72 gene expression progressively in­­creasesin human skeletal muscle during prolonged, exhaustive exercise. Journal of Applied Physiology, 89: 1055-1060.
    6. Goo D, Kim JH, Park GH, Reyes D, Badillo J and Kil DY (2019) Effect of Heat Stress and Stocking Density on Growth Performance, Breast Meat Quality, and Intestinal Barrier Function in Broiler Chickens. Animals, 9(3): 107.
    7. Helms S (2004) Cancer prevention and therapeutics: Panax Altern Med Rev, 9(3): 259-74.
    8. Hooper PL and Hooper JJ (2005) Loss of defense against stress: Diabetes and heat shock proteins. Diabetes Technology and Therapeutic, 7: 204-208.
    9. Hu R, He Y, Arowolo MA, Wu S and He J (2019) Polyphenols as potential attenuators of heat stress in poultry production. Antioxidants, 8(3): 67.
    10. Ismaili M, Deldar H and Ansari Pirsarai Z (2011) Effect of peppermint powder and orange spring sweat on the relative expression of heat shock protein (HSP70) gene and some blood parameters of broiler chickens under heat stress conditions. Journal of Animal Science Research. Volume 26, Number 3. (in persian)
    11. Jahejo AR, Rajput N, Rajput NM, Leghari IH, Kaleri RR, Mangi R, Sheikh MK and Pirzado MZ (2016) Effects of heat stress on the performance of Hubbard broiler chicken. Cells, Animal and Therapeutics, 2: 1-5.
    12. Jahejo AR, Rajput N, Wen-xia T, Naeem M, Kalhoro DH, Kaka A, ... & Jia FJ (2019) Immunomodulatory and growth promoting effects of basil (Ocimum basilicum) and ascorbic acid in heat stressed broiler chickens. Pakistan Journal of Zoology, 51(3): 801.
    13. Lee K, Everts H and Beyen AC (2003) Dietary carvacrol lowers body gain but improves feed conversion in female broiler chickens. Journal of Applied Poultry Research, 12: 394-399.
    14. Lin ER, Cheng YH, Hsiao FSH, Proskura W, Dybus A and Yu YH (2019) Optimization of solid-state fermentation conditions of Bacillus licheniformis and its effects on Clostridium perfringens-induced necrotic enteritis in broilers. Brazilian Journal of Animal Science, 48: e20170298.
    15. Liu W, Yuan Y, Sun C, Balasubramanian B, Zhao Z and An L (2019) Effects of dietary betaine on growth performance, digestive function, carcass traits, and meat quality in indigenous yellow-feathered broilers under long-term heat stress. Animals, 9(8): 506.
    16. Livak KJ and Schmittgen TD (2001) Analysis of relative gene expression data using real-timequantitative PCR and the 2-ΔΔCt. Method, 25: 402-408.
    17. Rassouli A, Jahanian R and Idris MA (2010) Effect of genistein isoflavonoid on morphology of jejunal mucosa cells and ileal digestibility of nutrients in broilers. 4th Iranian Congress of Animal Sciences. (in Persian)
    18. Smaili M, Deldar H and Ansari Pirsarae Z (2016) Effect of Mentha piperita powder and Citrus aurantium extract on heat shock protein (HSP70) gene expression and some blood parameters of broilers chickens in heat stress condition. Journal of Animal Science Research, 26: 115-124. (in Persian)
    19. Tahami Z, Shalaei M and Hosseini SM (2018) Effect of use of mixture of herbal extracts on performance, carcass characteristics, blood serum metabolites and enzyme activity of broiler chickens. Iranian Journal of Animal Science Research, 9: 446-460. (in Persian)
    20. Tahmasebi Ah, Shariatmadari F and Karimi Tarshizi M (2011) Effect of using alcoholic extract of thyme, vitamin E and fat in the diet on serum cholesterol, egg yolk and the immune system of laying hens under heat stress. Journal of Medicinal Plants. The second period. Issue Ninth. Eleventh year. (in Persian)
    21. Tajodini M, Samadi F, Hashemi SR, Hassani S and Ghasemnejad A (2015) Effects of different levels of artichoke (Cynara scolymus) powder and vitamin E on performance and immune system response of broiler chickens. Iranian Journal of Medicinal and Aromatic Plant, 31: 92-101. (in Persian)
    22. Tanrıkulu GI, Erturk O, Yavuz C, Can Z and Çakır HE (2018) Chemical compositions, antioxidant and antimicrobial activities of the essential oil and extracts of Lamiaceae family (Ocimum basilicum and Thymbra spicata) from Turkey. International Journal of Secondary Metabolites, 4: 340-34.