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Effect of zinc nano-complex on bull semen quality after freeze-thawing process

Document Type : Research Paper

Authors

1 M.Sc. Departments of Animal Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Assistant Professor, Departments of Animal Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Assistant Professor, Departments of Animal Science, Abourayhan Campus, University of Tehran, Pakdasht, Iran

4 Assistant Professor, Nahadehaye Dami Jahed Co. Tehran, Iran

5 Associate Professor,, Departments of Animal Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran

6 Ehrare Hargh Company, Tehran, Iran

Abstract

The effect of zinc Nano-particles (Zn- nano- complex) on bull sperm quality after freeze-thawing process studied. Ejaculates collected from four Holstein bulls twice a week. On the day of semen collection, four ejaculates were pooled and diluted with Bioxcell extender containing 0, 10-6, 10-5, 10-4, 10-3, 10-2 molar of zinc Nano-complex and frozen. After thawing, sperm motility and motion parameters, plasma membrane integrity, abnormal morphology, plasma membrane functionality and mitochondrial activity were determined. The proportion of the total and progressive motile sperm, the plasma membrane integrity and proportion of the spermatozoa with abnormal morphology was not different among groups. Zinc Nano-complex groups represented a higher plasma membrane functionality than that of control group. Moreover, our flowcytometric data suggested that spermatozoa in the groups of zinc Nano-complex possessed higher mitochondrial activity as compared with the control group. Mitochondrial activity in 10-2  was higher than the 10-6 group. In conclusion, supplementation of zn Nano-complex, can improve the plasma membrane functionality and mitochondrial activity of bull spermatozoa in a dose dependent manner without any deleterious effect on motility parameters.

Keywords

References
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Animal Production
Volume 17, Issue 2 - Serial Number 2
Autumn & Winter
October 2015
Pages 371-380
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