Document Type : Research Paper


1 M.Sc. Student, Department of Animal Science, Faculty of Agricultural Science & Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Assistant Professors, Department of Animal Science, Faculty of Agricultural Science & Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Associate Professor, Department of Animal Science, Faculty of Agricultural Science & Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

4 Assistant Professor, Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran

5 Assistant Professor, Department of Animal Science, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran


The objective of this study was to evaluate the effect of the STO feeder layer on prepubertal Rhode Island Red rooster SSCs culture and proliferation in vitro. Testis cells from 30 prepubertal Rhode Island Red chicken (4-8 weeks of age), were individually separated and cultivated in the presence of  bFGF and LIF growth factors on four well plates with two treatments and three replicats and five observations per each. SSCs colonies appeared on the 5th day of culture. The number of SSCs colonies, cells/colony and colony area was measured on days 7 and 10 for both treatments. The result of the colony assay on the 7th day revealed significantly higher colony numbers as well as higher cell number/colony and colony area on the STO surface compared to colonies grown on surfaces without a feeder layer (P≤0.05). In contrast, the results of the colony assay on day 10 had declined for both treatments, as compared to day 7. Also, the C-KIT gene was not expressed which is an indication that colonies might be composed of SSCs. In conclusion, these results indicate that the use of the STO feeder layer influences the SSCs proliferation and maintenance of the prepubertal roosters in short-term culture.  


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