Efficiency of bacteriophage in biocontrol of enterococcal colonization of quail

Noori Raygani, Jalil; Karimi Torshizi, Mohammad Amir; Rahimi, Shaban; Madadgar, Omid

doi:10.22059/jap.2015.53373

Document Type : Research Paper

Authors

¹ Ph.D. Candidate, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

² Assistant Professor, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

³ Professor, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

⁴ Associate Professor, Department of Virology, Faculty of Veterinary, Tehran University, Tehran, Iran

https://doi.org/10.22059/jap.2015.53373

Abstract

Three experiments were carried out for isolation of bacteriophage against growth reducing Enterococcus faecalis and evaluation of its efficacy to control Enterococci colonization in intestine of Japanese quails. In experiment one, 60 Enterococci spp were isolated from gastrointestinal contents of adult quails, which among them seven isolates were selected based on preliminary tests. In experiment two, effect of oral challenge by the seven selected isolates along with Enterococcus faecalis ATCC51299- as positive control on growth performance of growing quails was evaluated. In experiment three, lytic bacteriophage was screened against Enterococcus faecalis using quails excreta and poultry house swage as a sources of phage. Finally, efficiency of bacteriophage therapy (10⁴ pfu/mL) in biocontrol of enterococcal colonization of intestine was evaluated in quails orally challenged by Enterococcus faecalis (10⁷ cfu/mL). Among seven isolates from the first experiment, an isolate which reduced the growth of growing quails and had the minimum mortality in comparison to negative control group (P<0.05) was final selected and identified at the species level using biochemical tests based of carbohydrates fermentation profile as Enterococcus faecalis. Oral administration of isolated bacteriophage against Enterococcus faecalis was able to reduce the intestinal enterococci colonization to a level comparable to negative control group (P<0.05). In conclusion, isolated bacteriophage against Enterococcus faecalis effectively reduced enterococci colonization in intestinal content of quails and in this way, a growth reducing factor in poultry could be biocontrolled.

Keywords

20.1001.1.20096776.1394.17.2.4.8

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Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X

Efficiency of bacteriophage in biocontrol of enterococcal colonization of quail

References

References

Volume 17, Issue 2 - Serial Number 2
Autumn & Winter
October 2015
Pages 199-209

Efficiency of bacteriophage in biocontrol of enterococcal colonization of quail

References

References

Volume 17, Issue 2 - Serial Number 2Autumn & WinterOctober 2015Pages 199-209

Volume 17, Issue 2 - Serial Number 2
Autumn & Winter
October 2015
Pages 199-209