Elham Javdan; Jamal Fayazi; Saleh Tabatabaei; Mohammadtaghi Baigi Nasiri
Volume 15, Issue 2 , October 2013, , Pages 101-107
Abstract
This study was conducted to detect polymorphism of gene BMP15, a member of Transforming GrowthFactor β (TGFβ) family which has a crucial role in controlling the ovarian follicles development,ovulation rate and fertility. Samples were randomly selected from 91 Najdi goats in 3 geographicallocations, ...
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This study was conducted to detect polymorphism of gene BMP15, a member of Transforming GrowthFactor β (TGFβ) family which has a crucial role in controlling the ovarian follicles development,ovulation rate and fertility. Samples were randomly selected from 91 Najdi goats in 3 geographicallocations, northwest, southeast and center of Khuzestan province. After DNA extraction, amplification of235 bp fragment of exon 2 of BMP15 gene was performed using specific primers. Sequence detectionwas executed after amplification of gene fragments. The association of BMP15 gene and litter size wasdone by SAS software. Results from sequencing were analyzed by Vector NTI software. The resultsidentified three mutations in bases 529 (T to G), 530 (C to G) and 576 (T to C). The largest litter sizebelongs to AA pattern. Point mutations in this gene will alter the Ovulation rate of the goat. Therefore,improving twining trait in Najdi population can be expected by marker assisted selection.
H. Moradi Shahrbabak; A. Asadi; P. Azizi; S. Elahian
Volume 14, Issue 2 , January 2012, , Pages 43-50
Abstract
Expression of the Growth Hormone receptor (GHR) gene which is located on the sixteenth chromosome of sheep and its binding with GH is essential for growth and fat metabolism. In this study, blood samples were collected from the jugular vein from 88 sheep of Kermani breed.DNA was extracted from blood ...
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Expression of the Growth Hormone receptor (GHR) gene which is located on the sixteenth chromosome of sheep and its binding with GH is essential for growth and fat metabolism. In this study, blood samples were collected from the jugular vein from 88 sheep of Kermani breed.DNA was extracted from blood sample using the modified salting out method for amplification of 155 bp fragment containing a part of exon 10 of GHR genes. Single Strand Conformation Polymorphism (SSCP) was used for genotyping. The vertical electrophoresis of PCR products was performed on 12 percent polyacrylamide gel, at 300 V, for 17 h at 4 ˚C. The silver-staining of gels, resulted identification of six genotypic patterns: 1, 2, 3, 4, 5 and 6 with frequencies of 9.09, 4.54, 13.63, 9.09, 35.22 and 28.40 percent, respectively. Analysis of variance was performed using SAS software and association of different patterns was not significant with growth traits.