Abuzar gholampour; Khalil Mirzadeh; Saleh TabatabaeiVakili
Volume 23, Issue 4 , January 2022, , Pages 583-592
Abstract
The study aimed to investigate the effect of asparagus extract on quality semen of Arabian rams at different storage times at a temperature of 4 degrees Celsius. Sperm collection was performed from 10 Arab rams with an average age of 2.5 years per week (once a week) for six weeks. After adding 0, 1.5, ...
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The study aimed to investigate the effect of asparagus extract on quality semen of Arabian rams at different storage times at a temperature of 4 degrees Celsius. Sperm collection was performed from 10 Arab rams with an average age of 2.5 years per week (once a week) for six weeks. After adding 0, 1.5, 3 and 4.5% asparagus extract to the semen diluent, sperm quality parameters were evaluated at 0, 24, 48, and 72 hours after storage in liquid condition. At zero hour, sperm viability in diluent had 3% asparagus extract was higher than diluent containing 0 and 4.5% extract (P<0.05). After 24 hours of semen storage, the parameters of total motility, progressive motility, plasma membrane integrity and viability were higher in sperm diluent containing 1.5 and 3% of the extract (P<0.05). Qualitative parameters of sperm in 48 hours after sperm collection and storage in diluent contain 3% of extract had significant and better performance (P<0.05). The highest rate of plasma membrane integrity, progressive motility and total sperm motility was observed in diluent containing 3% extract in 72 hours after sperm storage (P<0.05). According to the results of this study, the use of asparagus extract in the diluent at the rate of 3% improves the sperm quality of Arabian rams after cooling conditions.
vahidVahid mohammadi; seyed davood sharifi; Mohsen Sharafi; abdollah mohammadi sang cheshmeh
Volume 22, Issue 2 , June 2020, , Pages 271-279
Abstract
The effect of feeding L-carnitine during pre-puberty on the quality parameters of fresh and frozen-thawed semen by using 12 Ross broiler breeder males (12 weeks) for 18 weeks, in a completely randomized design with three treatments (0, 250 and 500 mg / kg of L-carnitine in the diet) and four replications ...
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The effect of feeding L-carnitine during pre-puberty on the quality parameters of fresh and frozen-thawed semen by using 12 Ross broiler breeder males (12 weeks) for 18 weeks, in a completely randomized design with three treatments (0, 250 and 500 mg / kg of L-carnitine in the diet) and four replications was performed. From the age of 26 to 29 weeks, semen collection was performed using abdominal massage. The sperms taken each time after dilution (with Beltsville diluent) were divided into two parts, one section was frozen and the other part was immediately examined. Motility (total and forward), viability, morphology, membrane functionality and lipid peroxidation parameters were evaluated. In fresh sperm, the correlation between L-carnitine and abnormalities was negative linear, and with viability was positive linear (P<0.05). Quadratic analysis was significant in forward Motility and MDA concentration (P<0.05). Birds that use diets containing L-carnitine, In terms of forward motility, viability, morphology and MDA concentrations in fresh sperm, And these traits, with the total motility and integrity of the plasma membrane of frozen sperm, were higher in comparison to the control group (P<0.05). Also, in the sperm after frozen-thawed, the correlation between L-carnitine and Motility (total and forward), viability and membrane integrity were positive linear (P<0.05), and the correlation between L-carnitine and MDA concentration was negative linear (P<0.05). The correlation between L-carnitine and Motility (total and forward), membrane integrity and MDA concentration were quadratic (P<0.05). According to the results, Dietary L-carnitine supplementation in pre-puberty improves the qualitative traits of sperm before and after freezing in the breeder broilers.
hossein pasha zanussi; farid shariatmadari; Mohsen sharafi; Hamed Ahmadi
Volume 22, Issue 1 , March 2020, , Pages 79-92
Abstract
This study was conducted to assess the effects of dietary flaxseed and sesame oils, on the semen parameters, fatty acid composition of sperm as well as the fertility and hatchability eggs from aged roosters. In a completely randomized design, 24 Ross-308 roosters (aged 45 week) assigned to ...
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This study was conducted to assess the effects of dietary flaxseed and sesame oils, on the semen parameters, fatty acid composition of sperm as well as the fertility and hatchability eggs from aged roosters. In a completely randomized design, 24 Ross-308 roosters (aged 45 week) assigned to four groups, comprising six replicates and one bird in each. The birds received different diets including basal diet (control), basal diet supplemented with 2% flaxseed oil (FO), basal diet supplemented with 2% sesame oil (SO) and basal diet supplemented with 1% flaxseed oil and 1% sesame oil (MO). The diets were iso-caloric and iso-nitrogenous, containing the same level of vitamin E. The roosters were fed diet for 60 days, during which semen samples were collected on 1st, 20th, 40th and 60th days and the samples were tested for different characteristics. The results indicated that different diets affected semen qualities, except semen volume and the morphology. The concentration, progressive motility, MDA as well as viability of sperms were significantly different during different times of the experiment. The sperm quality parameters including total and progressive motility as well as MDA turned out to improve in the roosters fed FO or MO. Furthermore, the integrity of sperm membrane, DHA and DPA concentration, as well as the fertility were higher in the treatment group containing FO. It seems that supplementation of aged rooster’s diet with flaxseed oil or mixed oils, together with vitamin E improves the semen qualities and it can be applied as an appropriate strategy to preserve the reproductive performance of aged rooters.
toktam sadat Vafa; heshmat Sepehri moghadam; Mozhdeh Emadi; Alireza Hasani Bafarani
Volume 21, Issue 4 , January 2020, , Pages 569-577
Abstract
To examine the effect of glycyrrhizic acid on lipid peroxidation and vital parameters of Holstein bull sperm after freeze-thawing process, semen samples were collected from four mature Holstein bulls twice a week using artificial vagina. Ejaculates were pooled in order to eliminate the individual ...
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To examine the effect of glycyrrhizic acid on lipid peroxidation and vital parameters of Holstein bull sperm after freeze-thawing process, semen samples were collected from four mature Holstein bulls twice a week using artificial vagina. Ejaculates were pooled in order to eliminate the individual effects of bull. Semen samples were divided into four equal groups (8 reps) including Zero (control), 20, 30 and 40 mg/ml of glycyrrhizic acid along with diluents based on egg yolk-citrate. Following cooling and equilibration stage, semen samples were stored in nitrogen tank for 30 days. After thawing procedure, level of malondialdehyde in sperm samples were measured by ELISA. Also, membrane integrity, motility and viability of sperm were examined. Statistical analysis was carried out using one-way ANOVA and post-hoc Tukey tests (p<0.05). According to the results, membrane integrity, motility and viability of sperm samples treated with concentration of 20, 30 and 40 mg/ml glycyrrhizic acid dose dependent manner significantly increased and level of malondialdehyde dose dependent manner significantly decreased ccompared to control groups (p<0.05). Therefore, use of glycyrrhizic acid in bull semen diluent can improve sperm vital parameters and decreases lipid peroxidation of sperm after freeze-thawing process.
Mehdi Noorani; Shaban Rahimi; Abdolhoseein Shahverdi; Mohsen Sharafi
Volume 20, Issue 2 , August 2018, , Pages 203-212
Abstract
The aim of this study was cloning of the beta subunit of human chorionic gonadotropin in an appropriate vector for production of transgenic chicken trrough sperm mediated gene transfer. In this regard, transgenic chicken production tecnology has taken into consideration for having many advantages such ...
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The aim of this study was cloning of the beta subunit of human chorionic gonadotropin in an appropriate vector for production of transgenic chicken trrough sperm mediated gene transfer. In this regard, transgenic chicken production tecnology has taken into consideration for having many advantages such as short generation times, the large number of production of offspring and suitable pattern of protein glycosylation. To date, no study has been conducted on the cloning of the beta subunit of human chorionic gonadotropin for rooster sperm. For this purpose, the hormone beta subunit were amplified by a specific primer pairs, and cloned in T vector. The recombinant plasmid was transformed into Competent E. coli cells and colonies that containing recombinant plasmids were selected by colony PCR.The validity of extracted plasmid were analyzed by enzyme digestion and sequencing. The beta chain of T vector was isolated and was cloned again into pcDNA3.1 + expression vector. The results of enzyme analysis and sequencing indicated that recombinant plasmid pCDNA3.1 +/βhCG were cloned with the correct sequence and completely matched up with human chorionic gonadotropin beta subunit gene that can be concluded that it has sutible stracture for sperm mediated gene transfer.
Volume 19, Issue 1 , May 2017, , Pages 223-232
Abstract
In order to investigate the effect of different levels of Saturieja khuzistanica powder on the histomorphometric of testis, 36 native roosters (40 week age and BW 2039±293gr) was used. Three levels of Saturieja khuzistanica powder (0, 20 and 40g/kg) over 8 weeks was added to base diet. After ...
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In order to investigate the effect of different levels of Saturieja khuzistanica powder on the histomorphometric of testis, 36 native roosters (40 week age and BW 2039±293gr) was used. Three levels of Saturieja khuzistanica powder (0, 20 and 40g/kg) over 8 weeks was added to base diet. After eighth weeks, roosters were slaughtered and testes were removed from abdominal cavity. Tissue sections of testis after stained by Hematoxylin-Eosin were used to measure number of primary spermatocyte, secondary spermatocyte, spermatid, Sertoli cells, number of tubules and testicular vessels using optical microscope. In treatment with 40gr Saturieja khuzistanica powder per kg diet, number and density of vessels in testicular section were decreased compared to the control group (P< 0.05). According to these results, adding 40 gr/kg Saturieja khuzistanica powder to diet will maintain Sertoli cells and also increase primary spermatocyte, secondary spermatocyte and spermatid in comparison with the control group.
Arash Kheradmand
Volume 19, Issue 1 , May 2017, , Pages 233-244
Abstract
In this study antioxidant effects of olive leaf extract (OLE) in comparison to vitamin C and E on chilled-stored (at 5 °C) bovine sperm was evaluated. The sperm samples collected from epididymis of ten Holstein bovine testis with 3-5 years old, and were diluted with egg yolk and sodium citrate containing ...
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In this study antioxidant effects of olive leaf extract (OLE) in comparison to vitamin C and E on chilled-stored (at 5 °C) bovine sperm was evaluated. The sperm samples collected from epididymis of ten Holstein bovine testis with 3-5 years old, and were diluted with egg yolk and sodium citrate containing 0.5, 1.0 and 2.0 mg/ml of OLE as well as 2 mg/ml vitamin E and C. Addition of OLE significantly reduced sperm motility (P<0.05). Vitamin E significantly improved sperm membrane integrity compared to the other groups (P<0.05). TBARS in 2 mg/ml of OLE treatment was significantly less than that in other treatments (P<0.05). The results showed that addition of OLE to bovine sperms stored at 5 ͦ C for 48 h negatively affected sperm motility despite of a decrease in lipid peroxidation, and therefore, it is not suitable extender to be used for bovine spermatozoa.
Arash Kheradmand
Volume 18, Issue 4 , December 2016, , Pages 841-852
Abstract
This study was carried out to evaluate the effects of α-tocopherol, BSA and vitamin C on different caprine sperm parameters following incubation at 5 °C for 48 h. The treatments were as follows: 5 and 10 IU/ml of α-tocopherol, 4 and 8 mg/ml of BSA, as well as 3 and 6 mg/ml of vitamin ...
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This study was carried out to evaluate the effects of α-tocopherol, BSA and vitamin C on different caprine sperm parameters following incubation at 5 °C for 48 h. The treatments were as follows: 5 and 10 IU/ml of α-tocopherol, 4 and 8 mg/ml of BSA, as well as 3 and 6 mg/ml of vitamin C, and control group without any antioxidant. Total sperm motility, progressive motility and HOS test were assessed at h 0, 24 and 48. Using 4 mg of BSA and 5 IU of vitamin E increased sperm motility and plasma membrane integrity compared to other antioxidants (P<0.05). Although, higher concentrations of these antioxidants relatively enhanced sperm quality parameters, however, it was still greater than the control group (P<0.05). Different concentrations of vitamin C did not affect sperm quality parameters. According to the results of the present study, addition of BSA and vitamin E to buck semen improved total and progressive sperm motility as well as plasma membrane integrity during 48 h after incubation at 5 °C.
behrooz shokrani; morteza mehri; amir fattah; mohsen sharafi; fatemeh shirmohammad
Volume 18, Issue 4 , December 2016, , Pages 853-865
Abstract
Effects of dietary rosemary (Rosmarinus officinalis) essential oil were evaluated on the semen quality of broiler breeder rooster using sixteen 24-wk Ross 308 male birds. Experimental groups were consisting of four treatments (0, 50, 100 and 200 mg rosemary essential oil/kg of feed) with four replicates ...
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Effects of dietary rosemary (Rosmarinus officinalis) essential oil were evaluated on the semen quality of broiler breeder rooster using sixteen 24-wk Ross 308 male birds. Experimental groups were consisting of four treatments (0, 50, 100 and 200 mg rosemary essential oil/kg of feed) with four replicates per each group. Semen samples were collected on days 0, 14, 28, 42 and 56 of experiment. Results showed that 100mg rosemary significantly improved several traits of sperm such as Amplitude of Lateral Head Displacement (ALH), Average Path Velocity (VAP), and Straight Line Velocity (VSL) on days 42 and 56, and sperm Membrane Integrity (MI), sperm viability, Linearity (LIN) and Total Motility (TM) on day 56 (P<0.05) and significantly decreased dead sperms (P<0.05). However, sperm concentration, apoptosis and morphological changes, Sperm Track Straightness (STR), Curvilinear Velocity (VCL), Progressive motility (PM) and malondialdehyde (MDA) level were not affected by experimental treatment. It can be concluded that 100 mg rosemary essential oil/kg of diet could be efficient for improvement of some qualitative sperm parameters.
Raana Jahanbin; Parisa Yazdanshenas; Mehdi Amin Afshar; Abdollah Mohammadi Sangcheshmeh; Hamid Varnaseri; Mohammad Chamani; Mohammad Hasan Nazaran; Mohammad Reza Bakhtiyarizadeh
Volume 17, Issue 2 , October 2015, , Pages 371-380
Abstract
The effect of zinc Nano-particles (Zn- nano- complex) on bull sperm quality after freeze-thawing process studied. Ejaculates collected from four Holstein bulls twice a week. On the day of semen collection, four ejaculates were pooled and diluted with Bioxcell extender containing 0, 10-6, 10-5, 10-4, ...
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The effect of zinc Nano-particles (Zn- nano- complex) on bull sperm quality after freeze-thawing process studied. Ejaculates collected from four Holstein bulls twice a week. On the day of semen collection, four ejaculates were pooled and diluted with Bioxcell extender containing 0, 10-6, 10-5, 10-4, 10-3, 10-2 molar of zinc Nano-complex and frozen. After thawing, sperm motility and motion parameters, plasma membrane integrity, abnormal morphology, plasma membrane functionality and mitochondrial activity were determined. The proportion of the total and progressive motile sperm, the plasma membrane integrity and proportion of the spermatozoa with abnormal morphology was not different among groups. Zinc Nano-complex groups represented a higher plasma membrane functionality than that of control group. Moreover, our flowcytometric data suggested that spermatozoa in the groups of zinc Nano-complex possessed higher mitochondrial activity as compared with the control group. Mitochondrial activity in 10-2 was higher than the 10-6 group. In conclusion, supplementation of zn Nano-complex, can improve the plasma membrane functionality and mitochondrial activity of bull spermatozoa in a dose dependent manner without any deleterious effect on motility parameters.