farid moslemipur; Tahereh Aftabi; Shahriar Maghsoudlou; Ebrahim Gholamalipur Alamdari
Volume 24, Issue 2 , July 2022, , Pages 201-211
Abstract
Effect of using eucalyptus (Eucalyptus SPP) leaf chloroform oral extract (EE) and black pepper (Piper nigrum) powder (BP) in diet on growth performance, carcass traits, blood parameters and microbial populations of intestine and syrinx was investigated in a 42 days trial with 160 Cobb 500 day-old chicks ...
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Effect of using eucalyptus (Eucalyptus SPP) leaf chloroform oral extract (EE) and black pepper (Piper nigrum) powder (BP) in diet on growth performance, carcass traits, blood parameters and microbial populations of intestine and syrinx was investigated in a 42 days trial with 160 Cobb 500 day-old chicks divided into four treatments and four replicates as a completely randomized design. The treatments were 1- basal diet (control), 2- basal diet and adding 0.1 % EE solution in drinking water, 3- diet having 0.2% BP, and 4- diet having 0.2% BP and 0.1% EE solution in drinking water. Over the study, feed intake of chickens consumed oral EE or BP in diet and/or the combination of them was lower than chickens in control group (P<0.05). Weight gain and feed conversion ratio of chickens were not affected by the treatments. Oral EE resulted in greater percentile lungs weight and lower heart and abdominal fat weights in chickens (P<0.05). Using oral EE or BP in diet and/or the combination of them caused a significant decrease in serum concentrations of glucose, cholesterol and uric acid in chickens (P<0.05). Hematological parameters of chickens were not affected by the experimental treatments. Chickens consumed oral EE or BP in diet and/or the combination of them showed a significant lower intestinal E-coli population than control birds (P<0.05). Furthermore, using oral EE caused a decrease in Mycoplasma population of broilers Syrinx (P<0.05). The results showed that using oral EE leads to lowered abdominal fat and microbial populations of intestine and syrinx while adding BP in diet leads a decrease in microbial populations of intestine in broiler chickens.
Farzad Bastami; Ali Khatibjoo; Saifali Varmaghany
Volume 24, Issue 1 , April 2022, , Pages 67-80
Abstract
The effects of valine supplementation on performance, immunity and behavoural indices of broiler chickens reared under heat stress condition was evaluated. Total of 420 male and female Ross-308 broiler chickens were allocated to 6 treatments with 5 replicates in a completely randomized design. Experimental ...
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The effects of valine supplementation on performance, immunity and behavoural indices of broiler chickens reared under heat stress condition was evaluated. Total of 420 male and female Ross-308 broiler chickens were allocated to 6 treatments with 5 replicates in a completely randomized design. Experimental groups were as fallows: positive control (fed basal diet and reared under Ross-308 recommended rearing temperature), Nagative control (fed basal diet and reared under heat stress (HS)), and four treatment groups fed basal diet supplemented with 5, 10, 15 and 20 percent valine above the Ross-308 valine recommendation and reared under HS. Heat stress significantly decreased feed intake, body weight and European production efficiency factor (EPEF), antibody titer against NewCastle, lymphocyte percentage, serum total protein concentration and alkaline phosphatase and lactate dehydrogenase activities and increased heterophile percentage, heterophile to lymphocyte ratio, rectal temperature and tunic immobility of broiler chickens (P < 0.05), but 5% valine supplementation improved BW of broiler chicken at the end of grower and BW and EPEF at the end of total period; 10% valine supplementation increased relative weight of breast and thigh muscle and 15 and 20% valine supplementation increased lymphocyte and decreased heterophile percentage as compared to control group (P < 0.05). In conclusion, 5% valine supplementation improved final body weight and feed convertion ratio of heat-stressed broiler chikens and 15 and 20% valin inclusion improved blood immune cell percentages but had no effect on humoral immune response and tunic immobility test of HS broiler chickens.
Seyed Abdoullah Hosseini; Amir Hossein Alizadeh-Ghamsari; Houshang Lotfollahian; Majid Tavakkoli; Hoda Javaheri Barfourooshi
Volume 22, Issue 1 , March 2020, , Pages 93-103
Abstract
In order to evaluate the effect of different levels of corn gluten feed on performance, immune responses, intestinal morphology and some blood serum biochemical parameters of broiler chickens, an experiment was conducted using 500 male Ross 308 broiler chickens in a completely randomized design ...
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In order to evaluate the effect of different levels of corn gluten feed on performance, immune responses, intestinal morphology and some blood serum biochemical parameters of broiler chickens, an experiment was conducted using 500 male Ross 308 broiler chickens in a completely randomized design with four treatments, five replicates and 25 birds per replication. The experimental treatments included levels of zero (control), 2.5, 5 and 7.5 percent corn gluten feed in the diet which fed to broilers from 15 to 42 days of age. The results showed that dietary inclusion of corn gluten feed up to 7.5 percent had no significant effect on average weight gain, feed intake and feed conversion ratiocompared to control treatment. Also, jejunal morphological indices and blood biochemical parameters including triglyceride, cholesterol, albumin, phosphorus and alkaline phosphatase concentrations, livability, European broiler index and feed cost per kg of weight gain were not affected by dietary treatments. Antibody titer in response to sheep red blood cell (SRBC) injection was higher in treatment containing 2.5 percent corn gluten feed comparing to other treatments (P<0.05). Based on the obtained results, corn gluten feed can be included in grower and finisher diets of broilers (15 to 42 days of age) up to level of 7.5 percent without any adverse effect on performance.
Kobra Sookhtehzari; Ali Khatibjoo; Hoshang Jafari; Mohammad Akbari-Gharaei; Kamran Taherpour
Volume 21, Issue 4 , January 2020, , Pages 533-543
Abstract
The effects of high stocking density and threonine (Thr) supplementation on performance and immunity in broiler chickens was evaluated. In this experiment 495 male Ross-308 broiler chickens were allocated to 4 treatments with 5 replicates in a completely randomized design. The dietary treatment ...
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The effects of high stocking density and threonine (Thr) supplementation on performance and immunity in broiler chickens was evaluated. In this experiment 495 male Ross-308 broiler chickens were allocated to 4 treatments with 5 replicates in a completely randomized design. The dietary treatment consisted of: 1) basal diet as control with low density (10 birds/m2), 2) high density group (15 birds/m2 which fed basal diet) and 3 and 4) high density group which fed diet containing 10 and 20 percent Thr higher than requirment, respectively. The results showed that high stocking density decreased feed intake, body weight and feed conversion ratio at different period of production and increased European production efficiency factor (EPEF). Thr supplementation also reduced growth performance parameters (P < 0.05).Dietary treatments deseased antibody titer against NewCastle, but avian influenza virus, cell mediated immunity against phytoheamaglutinin, blood metabolites, blood cell count, and serum spartat aminotransferase and malondialdehyde concentration did noy change significantly. Serum alanine aminotransferase concentration in broiler chickens fed diet containing 20 percent Thr higher than requirment was higher than the others (P < 0.05). In conclusion, under the condition of this study (15 birds/m2), Thr supplementation (both of the levels) did not improved broiler chickens performance and immunity and more research is needed in this subject.
mehdi Vafaye valleh; nahid Karimi zandi; Farzaneh Bazmandegan Shomeyli
Volume 20, Issue 4 , February 2019, , Pages 513-525
Abstract
In this study, effects of the in ovo injection of Khazak egg yolk into the yolk of the Ross 308 eggs on some of offspring's inflammatory and immune indices as well as on the relative expression of intestinal and hepatic TNF-α and/or Zo-1 genes, were investigated. For this purpose, 250 fertile Ross ...
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In this study, effects of the in ovo injection of Khazak egg yolk into the yolk of the Ross 308 eggs on some of offspring's inflammatory and immune indices as well as on the relative expression of intestinal and hepatic TNF-α and/or Zo-1 genes, were investigated. For this purpose, 250 fertile Ross 308 eggs were randomly assigned into two equal experimental groups including test (In ovo injection of Khazak yolk) and control (In ovo injection of Ross yolk) group. After hatching, chickens were reared for six weeks under the same standard environmental conditions with exposure to some certain inflammatory stimuli between 21-28 days of age. Chicken’s blood and/or tissues samples were collected on days 10 and 42, and the samples were analyzed for the target traits. Results showed that Khazak yolk component caused a reduction in the levels of pro-inflammatory TNF-α cytokine in both offspring's liver and intestinal tissue (P<0.05). Compared to the control group, khazak yolk injection was found to enhance the titer of IgA natural antibodyas well as primary and secondary antibody titer response against sheep red blood cells (SRBC) and reduces both serum levels of offspring's CRP protein and liver ALT enzyme (P<0.05). According to the results of the present study, injection of Khazak native hen egg-yolk into the yolk of eggs from Ross 308 commercial broiler breeder can effectively suppress the expression of TNF-α inflammatory cytokine in the offspring's liver and intestinal tissue.
sayed abdoullah hosseini; mehdi amirsadeghi; amir hossein alizadeh-ghamsari; houshang lotfollahian; Mohammad Reza Solymani
Volume 19, Issue 4 , March 2018, , Pages 917-928
Abstract
The effects of two sources of organic zinc on performance and immune responses of broiler chickens were evaluated by using seven-hundred and fifty day-old Arian broiler chickens which randomly allocated in 30 experimental units in a completely randomized design as ...
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The effects of two sources of organic zinc on performance and immune responses of broiler chickens were evaluated by using seven-hundred and fifty day-old Arian broiler chickens which randomly allocated in 30 experimental units in a completely randomized design as a 2×3 factorial with two sources of organic zinc (zinc- methionine and zinc- glycine) and three levels of dietary zinc (40, 80 and 120 mg/kg). During the experiment, live body weight (LBW), feed intake (FI) and feed conversion ratio (FCR) were measured weekly. At the end of the experiment (age 42d) two birds from each replicate were bleed and total antibody titer against Sheep Red Blood Cell (SRBC), antibody titer against Newcastle Disease virus (ND) and differential count of white blood cells (WBC) were measured. Dietary inclusion of zinc-methionine improved LBW (day 42), FI (days 0-28) and FCR (days 0-14) in comparison with zinc- glycine (P<0.05), but did not influence on productive index and livability. Antibody titer against SRBC was higher when 80 mg/kg zincwas included in diet (P<0.05). Dietary inclusion of zinc-methionine improved performance of broiler chickens in comparison with zinc- glycine (P<0.05). Enhancement of some immune responses was observed by addition of 80 mg/kg organic zinc supplement to the diets. According to the results, using zinc-methionine improved broiler performance, so it should be suggested as a zinc source.
maryam bagheri Varzaneh
Volume 19, Issue 3 , November 2017, , Pages 613-625
Abstract
This study was conducted to investigate the effects of chromium-methionine (Cr-Met) supplementationon the blood and immune parameters of broiler under acute stress. A total of one hundred twenty six broilers were allocated to 3 treatments and 3 replicates (14 birds per replicate) in a completely randomized ...
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This study was conducted to investigate the effects of chromium-methionine (Cr-Met) supplementationon the blood and immune parameters of broiler under acute stress. A total of one hundred twenty six broilers were allocated to 3 treatments and 3 replicates (14 birds per replicate) in a completely randomized design. Treatments were: 1) negative control: i.e. basal diet without stress and Cr-Met, 2) positive control: basal diet with stress and without Cr-Met, 3) basal diet with stress and supplemented with 1000 ppb Cr-Met. Stress was induced from day 18 by addition of 1.5 mg dexamethasone per kg of the diet for one week followed by a withdrawal period until day 46. Blood samples were collected on day 25 and 46 of age. Results showed that stress increased blood glucose and lipid concentrations of serum (P < 0.05). The addition of Cr-Met decreased cholesterol, TG, LDL and VLDL on day 25 of age (P < 0.05). However, broilers fed Cr-Met had higher TG, LDL, VLDL and less HDL compared to other treatments on day 46 (P < 0.05). Administration of dexamethasone significantly elevated concentrations of white blood cell (WBC), heterophil (H), and heterophil to lymphocyte ratio (P < 0.05), while these parameters decreased by Cr-Met supplementation on day 25 (P < 0.05). In conclusion, supplemental Cr-Met improved broiler response to physiological stress during the acute stress period. However, broilers did not benefit from feeding Cr-Met supplementation after dexamethasone induced stress.
Volume 19, Issue 1 , May 2017, , Pages 189-200
Abstract
Effect of different levels of ginger (Zingiber officinale) and Protexin® probiotics on performance, humoral immune response and Ileal microbial population were evaluated using 360 birds in a completely randomized design as 2 × 3 factorial arrangement with 3 levels of ginger powder (0, 2.5 and ...
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Effect of different levels of ginger (Zingiber officinale) and Protexin® probiotics on performance, humoral immune response and Ileal microbial population were evaluated using 360 birds in a completely randomized design as 2 × 3 factorial arrangement with 3 levels of ginger powder (0, 2.5 and 5 g per kg), and 2 levels of probiotics (0 and 150 mg per kg) in 6 treatments and 5 replicates of 12 birds for 28 days. Birds consumed greater amount of diet containing 2.5 g/kg ginger (P<0.05). Birds fed diet containing 150 mg/kg of probiotics had higher feed intake and weight gain, and lower feed conversion ratio (P<0.05). Feed intake was higher in birds fed diet containing 2.5 g/kg ginger and 150 mg/kg probiotics compared with those birds received diet without ginger and probiotics (P<0.05). Consumption of probiotics, ginger and combination of probiotics and ginger increased antibody titer against secondary challenge with sheep red blood cells compared with those birds received diet without ginger and probiotics (P<0.05). Antibody titers against Newcastle virus were not affected by supplementation of ginger and probiotics. Supplementation of diet with probiotics alone and in combination with ginger increased Ileal lactic acid bacteria and total bacterial population compared with diet without ginger and probiotics (P<0.05). Based on the result of current study, supplementation of Japanese quail’s diet with 2.5 g/kg ginger and 150 mg/kg probiotics could improve feed intake, immune response and ileal microbial ecosystem.
Zahra Norreh; Ali Khatibjoo; Farshid Fattahnia; Mohammad Akbari-Gharaei
Volume 17, Issue 2 , October 2015, , Pages 269-279
Abstract
Effect of L-carnitine and butyric acid on the performance and immune responses of 192 broiler chickens (Ross 308) was investigated with three levels of L-carnitine (zero, 125 and 250 mg/kg) and two levels of butyric acid (2 g/kg) in a 2×3 factorial arrangement based on randomized complete block ...
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Effect of L-carnitine and butyric acid on the performance and immune responses of 192 broiler chickens (Ross 308) was investigated with three levels of L-carnitine (zero, 125 and 250 mg/kg) and two levels of butyric acid (2 g/kg) in a 2×3 factorial arrangement based on randomized complete block design with 6 dietary treatments, four replicates and eight birds per each. Broilers fed basal diet plus feed additives (L-carnitine and butyric acid) did not have significant differences on feed intake, body weight gain and feed conversion ratio when compred to control group. Broilers fed diets containing additives had higher blood lymphocyte count than control diet fed birds (P<0.05). Primary IgG tiitter (31d) against SRBC in broilers fed diet containing 125 mg/kg L-carnitine was higher than that of the control birds (P<0.05). Skin thickness in response to PHA in broielrs fed diet containing 250 mg/kg L-carnitine alone and 125 and 250 mg/kg L-carnitine in combination with butyric acid was lower than that of control group (P<0.05). It was concluded that addition of 125 mg/kg L-carnitine and two g/kg butyric acid did not influenced broiler chicken performance while improved their immune response.
Farhad Mohammadi; Farzad Bagherzadeh Kasmani; Kamal Shojaian; Mehran Mehri; Mohammad Amir Karimi Torshizi
Volume 17, Issue 2 , October 2015, , Pages 301-309
Abstract
Effect of adding Hibiscus sabdariffa (HS) in diet on performance and immune response of broilers was examined during aflatoxicosis using a total of 192 day-old Ross 308 male chicks in a completely randomized design with four treatments, four replicates and 12 chicks per each replicate. Treatments were ...
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Effect of adding Hibiscus sabdariffa (HS) in diet on performance and immune response of broilers was examined during aflatoxicosis using a total of 192 day-old Ross 308 male chicks in a completely randomized design with four treatments, four replicates and 12 chicks per each replicate. Treatments were include: 1. negative control (without additives), 2. positive control (2.5 mg aflatoxin B1/kg), 3. diet containing Hibiscus sabdariffa(10 g HS/kg) and 4. diet contaminated with aflatoxin and containing Hibiscus sabdariffa (2.5 mg AFB1/kg + 10 g HS/kg). Effect of treatments on feed intake was not significant. Weight gain of chicks in positive control treatment was lower than those birds in other treatments (P<0.001). These birds had higher feed conversion ratio (P<0.001). Antibody titer against Newcastle disease virus and sheep red blood cell in birds fed positive control and containing Hibiscus sabdariffa diets were lower and higher than those birds in negative control group, respectively (P<0.001). The least skin thickness after challenging with dinitrochlorobenzene (DNCB) was observed in birds fed positive control diet (P<0.001). Relative weight of bursa of Fabricius in chicks fed positive control and Hibiscus sabdariffa diets was lower and higher than that of negative control group, respectively (P<0.001). Based on results of this experiment, supplementation of Hibiscus sabdariffa to aflatoxin contaminated diets could ameliorate the effects of toxin on growth performance and immunity response of broilers.
Majid Aftabi; Farzad Bagherzadeh Kasmani; Ghasem Jalilvan; Mehran Mehri; Mohammad Amir Karimi Torshizi
Volume 17, Issue 1 , April 2015, , Pages 131-140
Abstract
Effect of Protexin to diminish negative consequences of feeding aflatoxin B1 (AFB1) contaminated diet on performance, immune response, meat quality and ileal microbial flora were examined using 320 seven-day-old Japanese quails fed four treatments [control diet (without additives), diet containing 2.5 ...
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Effect of Protexin to diminish negative consequences of feeding aflatoxin B1 (AFB1) contaminated diet on performance, immune response, meat quality and ileal microbial flora were examined using 320 seven-day-old Japanese quails fed four treatments [control diet (without additives), diet containing 2.5 mg AFB1/kg, diet containing 150 mg Protexin/kg, diet containing 2.5 mg AFB1/kg+150 mg Protexin/kg] with four replicates and 20 birds each in a completely randomized design. Feed intake of birds fed probiotic diet was higher than those birds in control group (P<0.05). Weight gain in birds fed aflatoxin contaminated diet was lower than birds in other treatments (P<0.05). Humoral immunity response in quails related to aflatoxin and probiotic groups was lower and higher than control group, respectively (P<0.05). Skin thickness increase after 48h challenging with dinitrochlorobenzene (DNCB) in birds fed aflatoxin contaminated diet was lower than the other groups (P<0.05). Concentration of malondialdehyde (after freezing meat for 30 days) in the meat of aflatoxin fed birds was higher than the other birds (P<0.05). Population of E. coli and lactic acid bacteria in birds fed protexin or aflatoxin+protexin was lower and higher than the two other groups, respectively (P<0.05). Based on the result of this research, dietary inclusion of protexin probiotics into aflatoxin contaminated diet could improve immunity response and intestinal microbial population in Japanese quails.