Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X

The effect of wheat straw processed with cellulolytic bacteria isolated from gastrointestinal tract of horse on performance, digestibility, and fermentation and blood parameters in the fattening lambs

Document Type : Research Paper

Authors

Maryam Harsini 1
tahereh mohammadabadi 2
hosein Motamedi 3
Mohsen Sari 4
Asadolah Teimouri Yansari 5

1 12 Department of Animal Science, Agricultural Sciences and Natural Resources University of Khuzestan, P.O. Box 63517-73637, Mollasani, Ahvaz, Iran

2 Ramin Agriculture and Natural Resources University of Khuzestan, Ahwaz, Mollasani - Iran

3 3 Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, P.O. Box 61357-43169, Khuzestan, Iran. 3 Biotechnologies and Biological Science Research Center, Shahid Chamran University of Ahvaz, P.O. Box 61357-43169, Khuzestan, Ir

4 Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani- Iran.

5 4 Department of Animal Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 48181-68984, Sari, Iran

https://doi.org/10.22059/jap.2022.298310.623504
Abstract
effect of these bacteriaon the performance of fattening lambs, digestibility, fermentation and blood parameters. Thirty Afshari male lambs with an average weight of 32 ± 3 kg and age of four months were used in a completely randomized design. The experimental treatments included diets containing wheat straw processed with four bacterial strains isolated from the gastrointestinal tract of horse (Paenibacillus polymyxa L11, Paenibacillus polymyxa L12Enterobacter cloacae L2 and Escherichia coli Z2) for 6 weeks; and the control treatment. The results showed that bacterial processing increased crude protein and decreased NDF, iNDF and ADF content, as well as increased organic matter digestibility (OMD) and metabolizable energy (ME) of wheat straw compared to the control. The highest amount of OMD and ME was assigned toL11. Apparent digestibility of DM, NDF and ADF, concentrations and ratios of ruminal volatile fatty acid (VFA), ruminal pH and ammonia nitrogen were affected by bacterial processing. The highest nutrient digestibility was assigned to the L11 and and the lowest amount was assigned to the control treatment. The lowest amount of ruminal ammonia nitrogen was observed in L11 treatment. Bacterial treatments increased total VFA, propionate concentration and decreased rumen acetate concentration. According to the results, processing of wheat straw with cellulolytic bacteria isolated from the horse's gastrointestinal tract (especially L11) improved nutritional value of wheat straw. Therefore, it seems that bacterial processing can be a suitable strategyfor better use of crop by-product with low nutritional value.

Keywords

Bacteria
digestibility
performance
processing
wheat straw
  1. Abd El-Galil E (2014) Using biological additives to manipulate rumen fermentation and improve baladi goat’s performance. Egyptian Journal of Nutrition and Feeds, 17: 29-42.‏
  2. AOAC (1990) Association of Official Analytical Chemists, Official Methods of Analysis, 15th edn. Arlington, VA.
  3. Azizi-Shotorkhoft A, Mohammadabadi T, Motamedi H, Chaji M and Fazaeli H (2016) Isolation and identification of termite gut symbiotic bacteria with lignocellulose-degrading potential, and their effects on the nutritive value for ruminants of some by-products. Animal Feed Science and Technology,221: 234-242.
  4. Benchaar C, McAllisterTAand Chouinard PY (2008) Digestion, ruminal fermentation, ciliate protozoal populations, and milk production from dairy cows fed cinnamaldehyde, quebracho condensed tannin, or Yucca schidigera saponin extracts. Journal of Dairy Science, 91(12): 4765-4777.
  5. Borji M (2003) The Survey Possibility of Straw Polysaccharides and Lignin Degradation by Isolated Microbiota from Termites. PhD Thesis. Tarbiat Modares University, Tehran, Iran.
  6. Broderick GA and Kang JH (1980) Automated simultaneous determination of ammonia and totalamino acids in ruminal fluid and in vitro Journal of Dairy Science, 63: 64-75.
  7. Elenin EIA, El-Galil ERA, Etman KEI and El-Shabrawy HM (2016) Improvement of Rumen Fermentation and Performance of Growing Lambs by Adding Natural Microbial Resources. Asian Journal of Animal Sciences,10(3):202-212.‏
  8. ELmoghazy MM, Husain M, Tag EDH and Din HA (2015) Effect of sheep diets containing microbiological treated rice straw on blood parameters and nitrogen balance. Journal of Microbiology Research, 5(2): 46-56.
  9. Fon FN, Nsahlai IV and Scogings PF (2014) Extraction and comparison of fibrolytic enzyme additives from gut of 11 ungulates. African Journal of Biochemistry Research, 8(2): 31-38.‏
  10. Gomaa R, Gado H, El-Sayed H, and El Mawla SA (2012) Usage of treated rice straw with exogenous anaerobic bacterial enzymes (ZAD) for Ossimi sheep. Annals of Agricultural Sciences, 57(2): 183-190.
  11. HarsiniShakarami M, Mohammadabadi T, Motamedi H, Sari M and TeimouriYansari A (2019) Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw. Journal of Applied Microbiology, 127(2): 344-353.
  12. Huhtanen P, Kaustell K and Jaakkola S (1994) The use of internal markers to predict total digestibility and duodenal flow of nutrients in cattle given six different diets. Animal Feed Science and Technology,48: 211-227.
  13. Kumari NN, Reddy YR, Blummel M, Nagalakshmi D, Monika T, Reddy BVS and Reddy CR (2013) Growth performance and carcass characteristics of growing ram lambs fed sweet sorghum bagasse-based complete rations varying in roughage-to-concentrate ratios. Tropical Animal Health and Production, 45(2): 649-655.‏
  14. López S, Dijkstra J and France J (2000) 4 Prediction of Energy Supply in Ruminants, with Emphasis on. Forage Evaluation in Ruminant Nutrition, 63.‏

15. Menke KH and Steingass H (1988) Estimation of the energetic feed value obtained from development, 28: 7-55.

16. Menke KH, Raab L, Salewski A, Steingass H, 
Fritz D and Schneider W (1979) The estimation 
of the digestibility and metabolisable energy 
content of ruminant feedingstuffs from the gas 
production when they are incubated with rumen 
liquor in vitro. The Journal of Agricultural 
Science, 93: 217-222. 
17. Mohammadi-Mehr A, Ghasemi E and 
Khorvash M (2017) Effect of Partial 
Replacement of Alfalfa Hay with Wheat Straw 
on Digestibility and Growth Performance of 
Fattening Male Lambs. Research on Animal 
Production, 9(19): 113-128. 
18. Oba M and Allen MS (1999) Evaluation of the 
importance of the digestibility of neutral 
detergent fiber from forage: effects on dry 
matter intake and milk yield of dairy cows. 
Journal of Dairy Science, 82(3): 589-596. 
19. Okano K, Iida Y, Samsuri M, Prasetya B, 
Usagawa T and Watanabe T (2006) Comparison 
of invitro digestibility and chemical composition 
among sugarcane bagasses treated by four whiterot fungi. Animal Science Journal,77(3):308-313. 
20. Richardson JM, Wilkinson RG and Sinclair LA 
(2003) Synchrony of nutrient supply to the 
rumen and dietary energy source and their 
effects on the growth and metabolism of lambs. 
Journal of Animal Science, 81(5): 1332-1347. 
21. Russell JB, O'connor JD, Fox DG, Van Soest 
PJ and Sniffen CJ (1992) A net carbohydrate 
and protein system for evaluating cattle diets: I. 
Ruminal fermentation. Journal of Animal 
Science, 70(11): 3551-3561. 
22. Shaikh NM, Patel AA, Mehta SA and Patel ND 
(2013) Isolation and Screening of Cellulolytic 
Bacteria Inhabiting Different Environment and 
Optimization of Cellulase Production. 
Universal Journal of Environmental Research 
and Technology, 3(1): 39-49. 
23. Shekhar C, Thakur SS and Shelke SK (2010) 
Effect of exogenous fibrolytic enzymes 
supplementation on milk production and nutrient 
utilization in Murrah buffaloes. Tropical Animal 
Health and Production, 42(7): 1465-1470. 
24. Suárez BJ, Van Reenen CG, Gerrits WJJ, 
Stockhofe N, Van Vuuren AM and Dijkstra J 
(2006) Effects of supplementing concentrates 
differing in carbohydrate composition in veal 
calf diets: II. Rumen development. Journal of 
Dairy Science, 89(11): 4376-4386. 
25. Van Soest PV, Robertson JB and Lewis BA 
(1991) Methods for dietary fiber, neutral 
detergent fiber, and nonstarch polysaccharides 
in relation to animal nutrition. Journal of Dairy 
Science, 74(10): 3583-3597.

Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X
Volume 24, Issue 3
Autumn 2022
Pages 301-312

Home

Guide for Authors

Submit Manuscript

Reviewers

Contact Us