Document Type : Research Paper
Authors
1 Jahade Keshavarzi Lorestan - Iran
2 Associate Professor of Animal Science Department, Faculty of Agriculture, Lorestan University,Iran
3 Lorestan University
Abstract
To investigate the effect of rosemary powder on semen parameters and DNA fragmentation under heat stress, 40 native rooster aged 42 weeksand weighted of 2300 gramswere usedin a factorial experiment in randomized complete block design with two levels of temperature (normal and heat stress) and rosemary powder (0 and 7.5 gr/kg of diet) during 7 weeks. Heat stress and normal temperature was set 28±2ºC and 18-22º C,respectively.After two weeks adaptation,the semen sampleswere collected twice in a week from roosters during 5 weeks using dorso-abdominal massage method. The semen parameters including motility, viability ratio and DNA fragmentation were evaluated. The results showed that heat stress significantly reduced the progressive motility of sperm (type A) and with the addition of rosemary powder, sperm viability increased (P˂0.05). Addition of rosemary leaf powder at normal temperature decreased the DNA fragmentation ratio (P˂0.05). In roosters under heat stress,the DNA fragmentation rate of sperm increased and the damage rate decreased significantly with the feeding of rosemary powder (P˂0.01). According to the results of this study, adding rosemary powder to the diet of native old roosters under heat stress conditions can improve some reproductive traits
Keywords
1. Afonso Mde O SAM CEB RDP BSB R, et al. RP and Mancini-Filho J (2013) Phenolic compounds from Rosemary (Rosmarinus officinalis L.) attenuate oxidative stress and reduce blood cholesterol concentrations in dietinduced hypercholesterolemic rats. Nutrition & metabolism, 10(1): 1-9.
2. Agarwal A and Said TMJHru (2003) Role of sperm chromatin abnormalities and DNA damage in male infertility. Human Reproduction Update, 9(4): 331-345.
3. Al-Sereiti MAbu-Amer K and Sena P (1999) Pharmacology of rosemary (Rosmarinus officinalis Linn.) and its therapeutic potentials. Indian Journal of Experimental of Biology, 37(2): 124-30.
4. Azad MAK, Kikusato M, Maekawa T, Shirakawa H and Toyomizu M (2010). Metabolic characteristics and oxidative damage to skeletal muscle in broiler chickens exposed to chronic heat stress. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 155(3): 401-406.
5. Boonsorn T, Kongbuntad W, Narkkong NA and Aengwanich W (2010). Effects of catechin addition to extender on sperm quality and lipid peroxidation in boar semen. American-Eurasian Journal of Agricultural and Environmental Science, 7(3): 283-288.
6. Borghei-Rad SMZeinoaldini SZhandi MMoravej H and Ansari MJT (2017) Feeding rosemary leaves powder ameliorates rooster age-related subfertility. Theriogenology, 101: 35-43.
7. Cerolini SZaniboni LMaldjian A and Gliozzi TJT (2006) Effect of docosahexaenoic acid and α-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation. Theriogenology, 66(4): 877-886.
8. Kanter MAktas CErboga MJT and health i (2013) Heat stress decreases testicular germ cell proliferation and increases apoptosis in short term: an immunohistochemical and ultrastructural study. Toxicology and industrial health, 29(2): 99-113.
9. Li YCao YWang F and Li CJAh (2014) Scrotal heat induced the Nrf2-driven antioxidant response during oxidative stress and apoptosis in the mouse testis. Acta histochemica, 116(5): 883-890.
10. Malo CGil LGonzalez NMartínez FCano RDe Blas I, et al. (2010) Anti-oxidant supplementation improves boar sperm characteristics and fertility aftercryopreservation: comparison between cysteine and rosemary (Rosmarinus officinalis). Cryobiology, 61(1): 142-147.
11. Organization WH. World health statistics 2010. World Health Organization; 2010.
12. Ramezaninejad H and Roostaei Ali Mehr MJIJoAS (2017) Storage of rooster semen in liquid form using alcoholic extract of rosemary. Iranian Journal of Animal Science, 48(1): 51-59.
13. Sakkas D and Tomlinson M (2000). Assessment of sperm competence. In: Seminars in reproductive medicine; 2000: Copyright© 2000 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New …; p. 133-140.
14. Salces-Ortiz J, Ramón M, González C, PerezGuzman MD, Garde JJ, Garcia-Alvarez O, ... and Serrano MM (2015). Differences in the ovine HSP90AA1 gene expression rates caused by two linked polymorphisms at its promoter affect rams sperm DNA fragmentation under environmental heat stress conditions. PLoSOne, 10(2), e0116360.
15. Sanches-Silva ACosta DAlbuquerque TGBuonocore GGRamos FCastilho MC, et al. (2014) Trends in the use of natural antioxidants in active food packaging: A review, 31(3): 374-395.
16. Sanocka D and Kurpisz M (2004). Reactive oxygen species and sperm cells. Reproductive biology and endocrinology, 2(1): 1-7.
17. Shafigh H, Shakeri M, Zeinoaldini S, Kohram H, Zhandi M and Moghbeli M (2016). Improving sperm cryopreservation of rooster by using rosemary alcoholic essential oil. Animal Production, 18(3): 615-624.
18. Smith R, Kaune H, Parodi D, Madariaga M, Ríos R, Morales I and Castro A (2006). Increased sperm DNA damage in patients with varicocele: relationship with seminal oxidative stress. Human Reproduction, 21(4): 986-993.
19. Tousson E, Bayomy MF and Ahmed AA (2018). Rosemary extract modulates fertility potential, DNA fragmentation, injury, KI67 and P53 alterations induced by etoposide in rat testes. Biomedicine & Pharmacotherapy, 98: 769-774.
20. Türk G, Çeribaşı AO, Şimşek ÜG, Çeribaşı S, Güvenç M, Kaya ŞÖ ... and Tonbak F (2016). Dietary rosemary oil alleviates heat stressinduced structural and functional damage through lipid peroxidation in the testes of growing Japanese quail. Animal reproduction science, 164: 133-143. 21. Yang HW, Hwang KJ, Kwon HC, Kim HS, Choi KW and Oh KS (1998). Detection of reactive oxygen species (ROS) and apoptosis in human fragmented embryos. Human reproduction (Oxford, England), 13(4): 998-1002.
22. Zadeh ZT, Shariatmadari F, Sharafi M and Torshizi MAK (2020). Amelioration effects of n-3, n-6 sources of fatty acids and rosemary leaves powder on the semen parameters, reproductive hormones, and fatty acid analysis of sperm in aged Ross broiler breeder roosters. Poultry science, 99(2): 708-718. 23. Zanganeh Z, Zhandi M, Zare-Shahneh A, Najafi A, Nabi MM and Mohammadi-Sangcheshmeh A (2013). Does rosemary aqueous extract improve buck semen cryopreservation?. Small Ruminant Research, 114(1): 120-125.
24. Zarghi H, Golian A and Kermanshahi H (2015). The effect of rosemary hydro-alcoholic (Rosmarinus officinalis L.) extract on performance and egg quality in laying hens. Iranian Journal of animal Science, 46(1): 1-8.
25. Zhai W, Neuman SL, Latour MA and Hester P Y. (2007). The effect of dietary L-carnitine on semen traits of white leghorns. Poultry Science, 86(10): 2228-2235.
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