Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X

Changes of testis histology parameters and relative expression of TGF-4 & StAR genes in roosters fed CoQ10 and omega 3 fatty acids

Document Type : Research Paper

Authors

hamid reza salari 1
Yousef Jafari Ahangari 2
Zarbakht Ansari Pirsaraie 3

1 , Animal Science Department, College of Agriculture & Natural Resources, University of Gorgan, Iran

2 Animal Science faculty, Gorgan university agricultural sciences & natural resources

3 Sari university of agricultural sciences & natural resources

https://doi.org/10.22059/jap.2021.314795.623578
Abstract
In this research, the effect of feeding coQ10 and omega 3 fatty acids on testicular histology and relative expression of TGF-b4 & StAR genes in Hubbard roosters was investigated. Forty-eight roosters, at fifty weeks age, were randomly divided into four groups and were subjected to the basal diet supplemented with  omega-3 (30gr), coQ10 (400mg) and  omega-3+ coQ10 (30gr+400mg) per day for eight successive weeks. At the end of the trial, all birds were killed and two samples were collected from the same testicle one of which was processed for histology, whereas another was snap-frozen in liquid nitrogen to assess relative gene expression. Results showed that seminiferous tubule diameters of experimental groups were significantly improved compared to the control group (P<0.05). However, leydig cell numbers and sertoli cells were not significantly affected. The relative expression of TGF-b4 genes were significantly decreased in three groups compared to control (P<0.05). Birds fed coQ10 and omega-3 had significantly higher StAR transcript level compared to other groups (P<0.05). Results of the present study showed that addition of a mixture of coQ10 and omega-3 fatty acids to rooster`s feed, lead to improvement in testicular histology and genes expression related to spermatogenesis.

Keywords

Omega 3 fatty acids
Fertility
Leydig cells
seminiferous tubule diameters
Sertoli cells

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Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X
Volume 23, Issue 2
Summer 2021
Pages 293-302

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