Document Type : Research Paper
Authors
1 MS Student of Animal genetic and Breeding, Faculty of Animal Science and Food Technology, Agricultural science and Natural Resources University of Khuzestan, Iran
2 Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Ahvaz, Iran
3 Assistant Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Ahvaz, Iran
4 Associate Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Ahvaz, Iran
Abstract
The effects of two different extenders were investigated (Tris- egg yolk and Andromed extenders) on semen quality and sex ratio during the dilution and freezing process in Holstien bull by using the real-time qPCR technique. The experiment was conducted using four 4-years old Holstien bulls for a period of four weeks in a completely randomized design. The PLP and SRY genes were amplified to isolate the specific fragments of X- and Y- chromosome sequences, respectively. Using Andromed diluent in comparison with Tris- eggs yolk improved semen quality during freezing and thawing. Using Andromed extender significantly increased the PLP gene expression (1.43±0.16), (P= 0/01) and reduced SRY gene expression (0.64±0.11), (P= 0/009). Using Tris- egg yolk extender led to a significant reduced in expression of PLP gene (0.3±0.06), (P< 0/0001) and s increased SRY gene expression during freezing (1.19±0.05), (P= 0/001). Based on the results, sperm dilution with Andromed extender and frozen semen with Tris-egg yolk increase birth of female and male in dairy cow herd, respectively.
Keywords
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