Document Type : Research Paper

Authors

1 Department of Animal Science, Islamic Azad University, Maragheh branch, Maragheh, Iran

2 Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

Abstract

In the present study, the polymorphism of four loci including of TLR4, IL2, IgL and IFNγ, in 100 commercial broiler and layer chickens using PCR-RFLP technique and enzymes of Tsp509I, Sau96I and HphI were investigated. In TLR4 marker site, two alleles of C (two bands of 138 and 119 bp) and G (three bands of 119, 99 and 39 bp), In IL2 locus, two alleles of A (four bands of 465, 64, 40 and 31bp) and B (five bands of 454, 64, 40, 31 and 11 bp), in IgL locus, two alleles M (two bands of 173, 161 and two bands of 10 bp) and N (three bands of 161, 103,70 and two bands of 10 bp) and in IFNγ locus, two alleles E (one band of 129 bp) and F (two bands of 90 and 39 bp) were identified. In TLR4, IL2, IgL and IFNγ markers sites, the population structure of the total population including the Shannon information index 0.43, 0.62, 0.68 and 0.69, the Fixation index -0.19, 0.37, -0.18 and 0.17 and the observed heterozygosity index 0.32, 0.27, 0.58 and 0.41 were estimated, respectively. Regarding the presence of polymorphism at these sites and in the case of studying the immune responses of the observed genotypes for these sites, can be used as marker for genetic breeding programs of chickens for increase of resistance to disease.

Keywords

1.     قاسمیان ا، رحیمی میانجی ق، انصاری ز و کاظمی ح (1391) استفاده از نشانگر آنزیمی Tsp509Iبرای شناسایی چندشکلی‌های تک نوکلئوتیدی در ناحیه پروموتور ژن اینترفرون گاما در مرغ­های مولد ایستگاه اصلاح نژاد مرغ بومی مازندران، پنجمین کنگره علوم دامی ایران، دانشگاه صنعتی اصفهان، اصفهان.
2.     مقصودی ص و پاکدل ع (1387) مروری بر روش‌های ژنتیکی مؤثر بر مقاوم‌سازی دام و طیور به بیماری­ها، نخستین همایش ملی صنعت دام و طیور، استان گلستان، گرگان.
3.     Butler JE (1998) Immunoglobulin diversity, B cell and antibody repertoire development in large farm animals. Revue Scientifique Et Technique (International Office of Epizootics) 17: 43-70.
4.     Corander J, Waldmann P and Sillanpää MJ (2003) Bayesian analysis of genetic differentiation between populations. Genetics 163: 367-374.
5.      Estess P, Nandi A, Mohamadzadeh M and Siegelman MH (1999) Interleukin 15 induces endothelial hyaluronan expression in vitro and promotes activated T cell extravasations through a CD44-dependent pathway in uivo. The Journal of Experimental Medicine 190: 9-19.
6.     Gavora JS (1990) Disease Genetics. In: Crawford RD (Eds.), Genetics and Breeding of Poultry. Elsevier, Amsterdam. PP. 805-846.
7.      Hawken RJ, Beattie CW and Schook LB (1998) Resolving the genetics of resistance to infectious diseases. Revue Scientifique Et Technique (International Office of Epizootics) 17: 17-25.
8.     Hoffmann I (2009) The global plan of action for animal genetic resources and the conservation of poultry genetic resources. World's Poultry Science Journal 65: 286-297.
9.       Jaiswal G, Kumar S, Prasad Y and Singh DP (2009) PCR-RFLP analysis of IL-2Rγ and IL-15Rα genes in Kadakanath native chicken. Journal of Applied Animal Research. 36: 239-242.
10.   Köllisch G, Kalali BN, Voelcker V, Wallich R, Behrendt H, Ring J, Bauer S, Jakob T, Mempel M and Ollert M (2005) Various members of the Toll‐like receptor family contribute to the innate immune response of human epidermal keratinocytes. Immunology 114: 531-541.
11.   Kramer J, Malek M and Lamont SJ (2003) Association of twelve candidate gene polymorphisms and response to challenge with Salmonella Enteritidis in poultry. Animal Genetics 34: 339-348.
12.   Kumar R, Kumar S, Singh DP and Gaur P (2007) DNA polymorphism at IL-2Rγ and IL-15Rα genes in Aseel native chicken. Journal of Applied Animal Research 32: 107-110.
13.   Lwelamira J, Kifaro GC, Gwakisa PS and Msoffe PLM (2008) Association of LEI0258 microsatellite alleles with antibody response against Newcastle disease virus vaccine and body weight in two Tanzania chicken ecotypes. African Journal of Biotechnology 7: 714-720.
14.   Malek M and Lamont SJ (2003) Association of INOS, TRAIL, TGF-b2, TGF-b3, and IgLgenes with response to Salmonella Enteritidisin poultry. Genetics Selection Evolution 35 (1): 99-111.
 
15.   Miller SA, Dykes DD and Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research. 16(3): 12-15.
16.   Ramasamy KT, Reddy MR and Murugesan S (2011) Toll-like receptor mRNA expression, iNOS gene polymorphism and serum nitric oxide levels in indigenous chickens. Veterinary Research Communications. 35: 321-327.
17.   Rees MJ and Nordskog AW (1981) Genetic control of serum immunoglobulin g levels in the chicken. Journal of Immunogenetics. 8: 425-431.
18.   Sheldon BL (2000) Research and development in 2000: Directions and priorities for the world’s poultry science community. Poultry Science. 78: 147-158.
19.   Thomas N and Joseph S (2012) Role of SLC11A1 gene in disease resistance. Biotechnology in Animal Husbandry. 28(1): 99-106.
20.   Tohidi R, Idris IB, Panandam JM and Bejo MH (2012) The effects of polymorphisms in IL-2, IFN-γ, TGF-β2, IgL, TLR-4, MD-2, and iNOS genes on resistance to Salmonella Enteritidis in indigenous chickens. Avian Pathology 41 (6): 605-612.
21.   Weir BS (1990) Genetic data analysis. In: Methods for discrete population genetic data (1st edn), Sinauer Assoc., Sunderland, MA, USA.
22.   Yeh FC, Rongcal Y and Boyle T (2000) POPGENE 1.32. A free program for the analysis of genetic variation among and within populations using co-dominant and dominant markers. Department of Renewable Resources, University of Alberta, Alberta, Canada.
23.   Yonash N, Heller ED, Hillel J and Cahaner A (2000) Detection of RFLP markers associated with antibody response in meat-type chickens: haplotype/genotype, single-band, and multiband analyses of RFLP in the major histocompatibility complex. Journal of Heredity. 91: 24-30.
24.   Zhou H, Buitenhuis AJ, Weigend S and Lamont SJ (2001) Candidate gene promoter polymorphisms and antibody response kinetics in chickens: Interferon-γ, Interleukin-2, and Immunoglobulin Light Chain. Poultry Science. 80: 1679-1689.