Document Type : Research Paper

Authors

1 Department of Animal Science, Faculty of Agriculture, Ilam University, Ilam, Iran

2 Former MSc. Student, Department of Animal Science, Faculty of Agriculture, Ilam University, Ilam, Iran

3 University assistant professor

Abstract

The purpose of this experiment was to examine the effects of Artemisia Annua L. essential oil (AAEO) and probiotic (lactobacillus acidophilus) as an alternative to antibiotic in feeding laying Japanese quails. A total of 180 46-d-old laying Japanese quails were randomly allocated to 4 dietary treatments in a completely randomized design with 4 replicates of 9 birds, and the experiment lasted up to 109 days of age. The experimental diets were: 1–basal diet as control group (without additive), 2–basal diet plus oxytetracycline (200 ppm), 3–basal diet plus AAEO (250 ppm) and 4– basal diet plus probiotic (4 CFU per kg diet). Results showed that the use of probiotic caused an increase in the egg mass, when compared with control and AAEOtreatments at entire period (P<0.05). The groups fed probiotic and AAEO showed a significant decrease in the serum glucose, cholesterol and LDL levels when compared with those fed antibiotic (P<0.05). Moreover, the group fed AAEO had low lymphocyte and high heterophil counts when compared with those fed diet supplemented with probiotic and antibiotic (P<0.05). In addition, the number of white blood cells and heterophil/lymphocyte ratio in AAEO group were higher than other groups (P<0.05). It can be concluded that lactobacillus acidophilus could be used as a growth promoter in feeding laying Japanese quails.

Keywords

ناصرمنش ح (1396) تأثیر پروبیوتیک (لاکتوباسیلوس اسیدوفیلوس) و اسانس آرتمیزیا بر عملکرد، ایمنی، قابلیت هضم مواد مغذی، مورفولوژی و جمعیت باکتری‌های ایلئوم بلدرچین‌های تخم‌گذار ژاپنی. پایان­نامه کارشناسی ارشد. دانشگاه ایلام.
2. ضمیری م ج (1380) فیزیولوژی دام. انتشارات حق شناس، رشت، 387 ص.
3. Abdul rahim S, Haddadin M, Hashlamoun E and Robinson R (1996) The influence of Lactobacillus acidophilus and bacitracin on layer performance of chickens and cholesterol content of plasma and egg yolk. British Poultry Science 37(2): 341-346.
4. Capcarova M, Weiss J, Hrncar C, Kolesarova A and Pal G (2010) Effect of Lactobacillus fermentum and Enterococcus faecium strains on internal milieu, antioxidant status and body weight of broiler chickens. Journal of Animal physiology and Animal Nutrition 94(5): 215-224.
5. Dalloul R, Lillehoj H, Shellem T and Doerr J (2003) Enhanced mucosal immunity against Eimeria acervulina in broilers fed a Lactobacillus-based probiotic. Poultry Science 82(1): 62-66.
6. Eberl G (2005) Inducible lymphoid tissues in the adult gut: recapitulation of a fetal developmental pathway? Nature Reviews Immunology 5(5): 413-420.
7. Edens F (2003) An alternative for antibiotic use in poultry: probiotics. Brazilian Journal of Poultry Science 5(2): 75-97.
8. Fang Y and Polk D (2011) Probiotics and immune health. Curr Opin Gastroenterol 27(6): 496-501.
9. Fathi M (2013) Effects of Lactobacillus cultures as probiotic on blood parameters, plasma enzymes activities and mortality in broiler chicken. Research Journal of Animal Science 7: 78-81.
10. Fu X and Liu Z (1997) Micro hemagglutination inhibition (HI) test. Page 97 in Handbook of Poultry Diseases Detection, ed. X.Q. Fu and Z.J. L, Ed. China Agriculture University Press, Beijingm, China.
11. Genedy S. Evaluation of using medicinal plants as feed additives in growing Japanese quail diet. in The 68th Scientific Conference of Polish Animal Production Society. Krakov. Poland. 2003.
12. Guclu BK (2011) Effects of probiotic and prebiotic (mannanoligosaccharide) supplementation on performance, egg quality and hatchability in quail breeders. Veterinary Journal of Ankara University 58(1): 27-32.
13. Li Z, Wang W, Liu D and Guo Y (2017) Effects of Lactobacillus acidophilus on gut microbiota composition in broilers challenged with Clostridium perfringens. Plos One 12(11): 1-16.
14. Mansi K, Amneh M and Nasr H (2007) The hypolipidemic effects of Artemisia sieberi (A. herba-alba) in alloxan induced diabetic rats. International Journal of Pharmacology 3(6): 487-491.
15. Matsuki T, Horai R, Sudo K and Iwakura Y (2003) IL-1 plays an important role in lipid metabolism by regulating insulin levels under physiological conditions. The Journal of Experimental Medicine 198(6): 877-888.
16. Mintzer DM, Billet SN and Chmielewski L (2009) Drug-induced hematologic syndromes. Advances in Hematology 2009.
17. Mohan B, Kadirvel R, Bhaskaran M and Natarajan A (1995) Effect of probiotic supplementation on serum/yolk cholesterol and on egg shell thickness in layers. British Poultry Science 36(5): 799-803.
18. Ouwehand A, Tiihonen K, Kettunen H, Peuranen S, Schulze H and Rautonen N (2010) In vitro effects of essential oils on potential pathogens and beneficial members of the normal microbiota. Veterinarni Medicina 55(2): 71-78.
19. Samyal ML, Kumar H, Khokra SL, Parashar B, Sahu RK and Ahmed Z (2011) Evaluation of antidiabetic and antihyperlipidemic effects of Artemisia dracunculus extracts in Streptozotocin-induced-diabetic rats. Pharmacologyonline 2: 1230-1237.
 
 
20. Shata F, Eldebaky H, Abd A and Hameed E (2014) Effects of camphor on hepatic enzymes, steroids and antioxidant capacity of male rats intoxicated with atrazine. Middle-East Journal of Scientific Research 22(4): 553-560.
21. Shimizu M, Hashiguchi M, Shiga T, Tamura H-o and Mochizuki M (2015) Meta-analysis: effects of probiotic supplementation on lipid profiles in normal to mildly hypercholesterolemic individuals. Plos One 10(10): 1-16.
22. Sonkusale P, Bhandarker A, Kurkare N, Ravikanth K, Maini S and Sood D (2011) Hepatoprotective activity of superliv liquid and repchol in CCl 4 induced FLKS syndrome in broilers. International Journal of Poultry Science 10(1): 49-55.
23.Verdian-Rizi M, Sadat-Ebrahimi E, Hadjiakhoondi A, Fazeli M and Pirali Hamedani M (2008) Chemical composition and antimicrobial activity of Artemisia annua L. essential oil from Iran. Journal of Medicinal Plants 1(25): 58-62.
24. Zeweil HS, Genedy SG and Bassiouni M (2006) Effect of probiotic and medicinal plant supplements on the production and egg quality of laying Japanese quail hens. in Proceeding of the 12th European poultry conference, ZWANS.
25. Zulkifli I, Abdullah N, Azrin NM and Ho YW (2000) Growth performance and immune response of two commercial broiler strains fed diets containing Lactobacillus cultures and oxytetracycline under heat stress conditions. British Poultry Science 41(5): 593-597.