Document Type : Research Paper

Authors

• Hengameh Vafaee ¹
• Mojtaba Hosseinpour Mashhadi2 ²

¹ M.Sc. Graduate, Department of Animal Science, Faculty of Agriculture, Mashhad Branch, Islamic Azad University, Mashhad - Iran

² Assistant Professor, Department of Animal Science, Faculty of Agriculture, Mashhad Branch, Islamic Azad University, Mashhad - Iran

Abstract

 This study was conducted to investigate the Single nucleotide polymorphism of STAT1 gene by PCR-RFLP technique and association of genotypes with milk production traits in Brown Swiss dairy cattle. The blood samples of ninety eight cows were collected randomly from tail vein. The DNA was extracted with Cinagene kit (PR881612C). Fragment of 314 bp of STAT1 gene was amplified by PCR and digested by Pag1 restriction enzyme. Genotype frequencies were calculated by PopGene software (version 1.31). The SAS 9.1 software was used for statistical analysis and the mean of traits was compared by Duncan test. The genotype frequencies of TT, TC and CC were 0.23, 0.25 and 0.52 respectively. The T and C allele frequency were 0.355 and 0.645 respectively. The expected heterozygosity was estimated 0.458. Result of Chi-square showed that the population was not in the Hardy Weinberg equilibrium (P<0.05). The means of traits for milk, percent and yield of fat and yield of protein were not significant but means of percent of protein was significant(P<0.05). The mean of milk, fat and protein yield traits for CC genotype were more than two other genotypes. The results of present study show that the T allele is minor frequency allele and C allele has positive effect on milk, fat and protein yield traits.

Keywords

• Brown Swiss breed
• Gene
• Milk production traits
• STAT1

20.1001.1.20096776.1395.18.3.1.4