Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X

The effects of feeding sequence and boiling barley on activity of hydrolytic enzymes in colonic contents of Arabian mare

Document Type : Research Paper

Authors

Ali Kiani
Ayoob Azizi shotor-khoft
https://doi.org/10.22059/jap.2016.58956
Abstract
Effects of feeding sequence and boiling of barley on digestibility of fiber and activity of hydrolytic enzymes in colonic contents of horse were investigated. The experiment carried out in a completely randomized design using six Arabian mares in a factorial cross-over with four consecutive periods. The concentrate part of the ration was offered either 30 min after (F-C) or 30 min before (C-F) forage feeding. Barley grain was either boiled (processed) or crushed (not processed). At the end of each period, fecal sample was collected directly from rectum and the activities of carboxymethyl cellulase, microcrystalline cellulase, filter paper degrading, and α-amylase enzyme were determined. Results showed that dry matter, neutral detergent fiber and acid detergent fiber intakes were significantly higher in C- F than those in F-C (P<0.05). Feeding sequence had no effects on digestibility of fiber and on activity of carboxymethyl cellulase, microcrystalline cellulase and filter paper degrading activity. Amylase activity was significantly increased in C-F and decreased by boiling barley (P<0.05). When processed barley was used, the activity of carboxymethyl cellulase decreased in F-C but increased in C-F. In conclusion, using F-C feeding sequence and boiling barley showed positive effects on the activity of hydrolytic enzymes in rectal contents of Aabian mares.

Keywords

Amylase
Arabian horse
carboxymethyl cellulose
feeding sequence
microcrystalline cellulose
cereal processing
  1. Agarwal  N, Agarwal  I, Kamra DN and Chaudhary LC (2000) Diurnal variations in the activities of hydrolytic enzymes in different fractions of rumen contents of Murrah Buffalo. Journal of Applied Animal Research. 18: 73-80.
  2. Agarwal N, Saxena J, Saha S, Chaudhary LC and Kamra DN ( 2004) Changes in fermentation characteristics, microbial populations and enzyme profile in the rumen of buffaloes affected by roughage level in the diet. Bubalusbubal. 111: 81-90.
  3. AOAC (2004) Official Methods of Analysis. Association of Official Analytical Chemists, Arlington VA, USA.
  4. De Fombelle A, Veiga L, Drogoul C and Julliand V (2004) Effect of diet composition and feeding pattern on the prececal digestibility of starches from diverse botanical origins measured with the mobile nylon bag technique in horses. Journal of Animal Science. 82: 3625-3634.
  5. Edouard N, Fleurance G, Martin-Rosset, W, Duncan P, Dulphy, JP, Grang S, Baumont R, Dubroeucq H, Perez-Barberia FJ and Gordon IJ (2008) Voluntary intake and digestibility in horses: effect of forage quality with emphasis on individual variability. Animal. 2(10): 1526-1533.
  6. Frape D (2004) Equine Nutrition and Feeding, 3rd Edition, Blackwell Publishing Ltd, Oxford, UK.
  7. Hoffman RM, Boston RC, Stefanovski D, Kronfeld DS and Harris PA (2003) Obesity and diet affect glucose dynamics and insulin sensitivity in Thoroughbred geldings. Journal of Animal Science. 81: 2333-2342.
  8. Holm J, Lundquist I, Bjrck I, Eliasson AC and Asp NG (1988) Degree of starch gelatinization, digestion rate of starch in vitro, and metabolic response in rats. The American Journal of Clinical Nutrition. 47: 1010-1016.
  9. Hristov AN, McAllister TA and Cheng KJ (1999) Effect of diet, digesta processing, freezing and extraction procedure on some polysaccharide-degrading activities of ruminal contents. Canadian Journal of Animal Science. 79: 73-81.
  10. Hudson JM, Cohen ND, Gibbs PG and Thompson JA (2001) Feeding practices associated with colic in horses. Journal of the American Veterinary Medical Association. 219: 1419-1425.
  11. Jassim RAMA (2006) Supplementary feeding of horses with processed sorghum grains and oats. Animal Feed Science and Technology. 125: 33-44.
  12. Julliand V, De Fombelle A, Drogoul C and Jacotot E (2001) Feeding and microbial disorders in horses: Part 3 – Effects of three hay:grain ratios on microbial profile and activities. Journal of Equine Veterinary Science. 21: 543-546.
  13. Julliand V, De Fombelle A and Varloud M (2006) Starch digestion in horses: The impact of feed processing. Livestock Science. 100: 44-52.
  14. Kamra DN, Saha S, Bhatt N, Chaudhary LC and Agarwal N (2003) Effect of diet one enzyme profile, biochemical changes and in sacco degradability of feeds in the rumen of buffalo. Journal of Animal Science. 16: 374-379.
  15. Kienzle E, Pohlenz J and Radicke S (1997) Morphology of starch digestion in the horse. Journal of Veterinary Medicine Series A. 44: 207-221.
  16. Martin C and Michalet-Doreau B (1995) Variations in mass and enzyme activity of rumen microorganisms: Effect of barley and buffer supplements. Journal of Food Agriculture and Environment. 67: 407-413. 
  17. Moore BE and Dehority BA (1993) Effects  of  diet  and  hindgut defaunation  on diet  digestibility  and  microbial  concentrations in the  cecum  and  colon  of  the  horse. Journal of Animal Science. 71: 3350-3358.
  18. Rowe JB, Lees MJ and Pethick DW (1994) Prevention of acidosis and laminitis associated with grain feeding in horses. The Journal of Nutrition. 124: 2742S-2744S.
  19. Rowe JB, Choct M and Pethick W (1999) Processing cereal grains for animal feeding. Australian Journal of Agricultural Research. Pp. 50-721.
  20. Santos AS, Rodrigues MAM, Bessa RJB, Ferreira LM and Roset W (2010) Understanding the equine cecum-colon ecosystem: current knowledge and future perspectives. Animal. 5: 48-56.
  21. Selmi B, Marion D, Perrier Cornet JM, Douzals JP and Gervais P (2000) Amyloglucosidase hydrolysis of high-pressure and thermally gelatinized corn and wheat starches. Journal of Agricultural and Food Chemistry. 48: 2629-2633.
  22. Silva AT, Wallace RJ and Orskov ER (1987) Use of particle-bond microbila activity to predict the rate and extent of fiber degradation in the rumen. British Journal Nutrition. 57: 407-415.
  23. Van Soest PJ (1994) Nutritional Ecology of the Ruminant, 2nd ed. Comstock, Cornell University Press, Ithaca, NY.
  24. Van Soest PJ and Mason VC (1991) The influence of the maillard reaction upon the nutritive value of fibrous feeds. Animal Feed Science and Technology. 32: 45-53.
  25. Vervuert I, Voigt K, Hollands T, Cuddeford D and Coenen M (2008) Effects of processing barley on its digestion by horses. The Veterinary Journal. 162: 684-688.
  26. Zeyner A, Geißler C and Dittrich A (2004) Effects of hay intake and feeding sequence on variables in faeces and faecal water (dry matter, pH value, organic acids, ammonia, and buffering capacity) of horses. Journal of Animal Physiology and Animal Nutrition. 88: 7-19.

Journal of Animal Production, Print ISSN: 2008-6776., Online ISSN: 2382-994X
Volume 18, Issue 4
Autumn 2016
Pages 749-760

Home

Guide for Authors

Submit Manuscript

Reviewers

Contact Us