Animal Production

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

Journal Metrics

News

Effect of osmolarity and glycerol different levels in soybean lecithin-based extender on bovine sperm quality after cryopreservation

Document Type : Research Paper

Authors

1 Ph.D. Student, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Associate Professor,Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

3 Associate Professor,Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

4 Assistant Professor, Department of Animal and Poultry Science, College of Abouraihan, University of Tehran, Pakdasht, Tehran, Iran

5 Associate Professor, Research Center for New Technology in Life Science Engineering, University of Tehran, Iran

Abstract

The effect of osmolarity and glycerol different levels in soybean lecithin-based extender on the bull sperm quality after cryopreservation was examined using six Holstein bulls in a 2 × 3 factorial trial based on completely randomized design, with three levels of osmolarity (250, 300 and 350 mOsml) and two levels of glycerol (5 and 7 %) .In general, semen samples were collected 36 times (from each bull, six times). After the initial evaluation of semen, samples were mixed together and assigned to each of treatments. After freezing and thawing process, parameters that were evaluated including; motion characteristics by CASA, viability, plasma membrane integrity and morphology. The results showed that frozen-thawed sperm in treatment of G7P300 had higher values than the other groups for total motility (69.50 %), progressive motility (48.89 %), lateral head displacement (3.69 µm/s), curvilinear velocity (168.80 µm/s) and straightness coefficient (61.89 %) (P≤0.05). In the treatments containing seven and five percent of glycerol and osmotic pressure of 350 mOsml, plasma membrane integrity (23.14 and 25.63 %, respectively) and sperm viability (58.70 and 64.60 %, respectively) were lower compared to other treatments (P≤0.05). But, in terms of morphology, G7P350 (92.34 %) and G5P350 (92.57 %) treatments were better than other treatments. The results of these experiment showed that the extender contained of seven percentage of glycerol with osmalarity of 300 or 250 mOsml was more efficient for cryopreservation of Holstein bull sperm.

Keywords

References
1 . Büyükleblebici S, Tuncer PB, Bucak M, Tasdemir U, Eken A, Büyükleblebici O, Durmaz E, Sarıözkan S, Endirlik B and Chaveiro A (2014) Comparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders. Cryobiology. 69: 74–78.
2 . Chelucci S, Pasciu V, Succu S, Addis D, Leoni GG, Manca ME, Naitana S and Berlinguer F (2015) Soybean lecithin–based extender preserves spermatozoa membrane integrity and fertilizing potential during goat semen cryopreservation. Theriogenology. 83: 1064–1074.
3 . Curry MR, Millar JD and Watson PF (1994) Calculated optimal cooling rates for ram and human sperm cryopreservation fail to confirm with empirical observations. Biology Reproduction. 51: 1014–21.
4 . Garzon-Perez C, Flores HF, Medrano A (2010) A simple osmotic stress test to predict boar sperm cryosurvival.Cryo Letters. 31(6):438-44.
5 . Guthrie HD, Liu J and Critser JK (2002) Osmotic tolerance limits and effects of cryoprotectants on motility of bovine spermatozoa. Biology of Reproduction. 67:1811–1816.
6 . Mazur P (1970) Cryobiology the freezing of biological systems. Science. 168: 939-944.
7 . Moce E, Purdy E and Graham JK (2010) Treating ram sperm with cholesterol-loaded cyclodextrins improves cryosurvival. Animal Reproduction Science. 118: 236–247.
8 . Rasul Z, Ahmed N and Anzar M (2007) Antagonist effect of DMSO on the cryoprotection ability of glycerol during cryopreservation of buffalo sperm. Theriogenology. 68: 813-819.
9 . Röpke T, Oldenhof H, Leiding C, Sieme H, Bollwein H and Wolkers WF (2011) Liposomes for cryopreservation of bovine sperm. Theriogenology. 76: 1465–1472.
10 . Rosato MP and Iaffaldano N (2013) Cryopreservation of rabbit semen: Comparing the effects of different cryoprotectants, cryoprotectant-free vitrification, and the use of albumin plus osmoprotectants on sperm survival and fertility after standard vapor freezing and vitrification. Theriogenology. 79: 508–516.
11 . SAS Institute, version 9.1 ( 2002) Cary, NC, USA.
12 . Sitaula R, Elmoazzen H, Toner M and Bhowmick S (2009) Desiccation tolerance in bovine sperm: a study of the effect of intracellular sugars and the supplemental roles of an antioxidant and a chelator, Cryobiology. 58: 322–330.
13 . Steinbach J and Foote RH (1967) Osmotic pressure and pH effects on survival of frozen bovine spermatozoa. Journal Dairy Science. 50: 205–213.
14 . Woelders H, Mathiijs A and Engel B (1997) Effects of trehalose, and sucrose, osmolality of the freezing medium, and cooling rate on viability and intactness of bull sperm after freezing and thawing, Cryobiology. 35: 93–105.
Animal Production
Volume 18, Issue 3
October 2016
Pages 593-601
Files
Share
How to cite
Statistics