Document Type : Research Paper

Authors

1 Ph.D. Student, Department of Animal Science, Faculty of Agriculture, International Campus, Ferdowsi University of Mashhad,

2 Professor, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad

3 Assistant Professor, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad

Abstract

The purpose of this study was to determine the effect of two levels of four commercial multi-enzymes on the apparent metabolizable energy corrected for nitrogen (AMEn) and digestibility of crude fat, crude protein, dry matter, and organic matter in broiler chickens through total excreta collection method. One hundred thirty male broiler chicks were assigned to 45 metabolic cages involving 9 treatments, and 5 replicates of 3 chicks each. A control diet was made so that wheat was the sole source of energy supply. Eight experimental diets were prepared with the addition of one or two recommended levels of each commercial multi-enzymes to the control diet. The chickens had free access to feed and water ad-libitum from 17 to 23 days of age. Gross energy, crude protein, crude fat, organic matter, and dry matter were measured in feed and excreta samples. Addition of one or two recommended levels of different commercial enzymes to diet significantly increased (P< 0.05) AMEn and apparent digestibility of fat, protein, dry matter, and organic matter in wheat. However, there was not a significant difference between the levels and the four types of added commercial enzymes on AMEn and nutrient digestibility values. The results revealed that metabolisable energy value of Falat wheat improved by 70 to 120 kcal per kg, regardless of the type and activity of the four commercial enzymes used in this study.

Keywords

1 . زرقی ح، گلیان ا، کرمانشاهی ح و عاقل ح (1389) اثر مکمل آنزیمی بر انرژی قابل متابولیسم ذرت، گندم و تریتیکاله با استفاده از روش­های جمع‌آوری کل فضولات و نشانگر در جوجه­های گوشتی. پژوهش‌های علوم دامی. 3: 112-105.
2 . کریمی ا، اسکات ت، کامیاب ع، نیکخواه ع و مرادی م (1381) اثر عمل‌آوری، سطح آنزیم و افزودن آنتی‌بیوتیک به جیره گندم‌دار بر روی مقدار انرژی قابل متابولیسم ظاهری، عملکرد و توسعه دستگاه گوارش جوجه­های گوشتی نر. علوم کشاورزی ایران. 33: 431-421.
 
3 . Adeola O and Cowieson AJ (2011) Board-invited review: Opportunities and challenges in using exogenous enzymes to improve non-ruminant animal production. Journal of Animal Science. 89: 3189-3218.
4 . Angkanaporn K, Choct M, Bryden WL, Annison EF and Annison G (1994) Effects of wheat pentosanase on endogenous amino-acid losses in chickens. Journal of the Science of Food and Agriculture. 66: 399-404.
5 . AOAC (1990) Official Methods of Analysis. Association of Official Analytical Chemists, Washington, DC. USA.
6 . Bedford MR and Apajalahti J (2001) Microbial interactions in the response to exogenous enzyme utilization. Enzymes in Farm Animal Nutrition. Pp. 299-315 (CAB International North America).
7 . Bedford MR and Classen HL (1992) Reduction of intestinal viscosity through manipulation of dietary rye and pentosan concentration is effected through changes in the carbohydrate composition of the intestinal aqueous phase and results in improved growth rate and food conversion efficiency of broiler chicks. Journal of Nutrition. 122: 560-569.
8 . Bedford MR (1995) Mechanism of action and potential environmental benefits from the use of feed enzymes. Animal Feed Science and Technology. 53: 145-155.
9 . Choct M and Annison G (1992) The inhibition of nutrient digestion by wheat pentosans. British Journal of Nutrition. 67: 123-132.
10 . Choct M, Huges RJ, Trimble RP, Angkanaporn K and Annison G (1995) Non-starch polysaccharide-degrading enzymes increase the performance of broiler chickens fed wheat and low apparent metabolisable energy. Journal of Nutrition. 125: 485-492.
11 . Choct M and Annison G (1992) Anti-nutritive effect of wheat pentosans in broiler chickens: Roles of viscosity and gut microflora. British Poultry Science. 33: 821-834.
12 . Crouch AN, Grimes JL, Ferket PR and Thomas LN (1997) Enzyme supplementation to enhance wheat utilization in starter diets for broilers and turkeys. Journal Applied Poultry Research. 6: 147-154.
13 . Engberg RM, Hedemann MS, Steenfeldt S and Jensen BB (2004) Influence of whole wheat and xylanase on broiler performance and microbial composition and activity in the digestive tract. Poultry Science. 83: 925-938.
14 . Esmaeilipour O, Moravej H, Shivazad M, Rezaian M, Aminzadeh S and Van Krimpen M (2012) Effects of diet acidification and xylanase supplementation on performance, nutrient digestibility, duodenal histology and gut microflora of broilers fed wheat based diet. British Poultry Science. 53: 235-244.
15 . Fowan WD, Jorgensen OB, Rasmussen PB and Wagner P (1993) Role of single activity xylanase enzyme omponents in improving feed performance in wheat based poultry diets. Agro-Food Industry Hi-Tech. Pp. 11-14.
16 . Hesselman K and Aman P (1986) The effect of β-glucanase on the utilization of starch and nitrogen by broiler chickens fed on barley of low or high viscosity. Animal Feed Science and Technology. 15: 83-93.
17 . Hughes RJ (2008) Relationship between digesta transit time and apparent metabolisable energy value of wheat in chickens. British Poultry Science. 49(6): 716-720.
18 . Kalmendal R and Tauson R (2012) Effects of a xylanase and protease, individually or in combination, and an ionophorecoccidiostat on performance, nutrient utilization, and intestinal morphology in broiler chickens fed a wheat-soybean meal-based diet. Poultry Science. 91: 1387-1393.
19 . Kiarie E, Romero LF and Ravindran V (2014) Growth performance, nutrient utilization, and digesta characteristics in broiler chickens fed corn or wheat diets without or with supplemental xylanase. Poultry Science. 93: 1186-1196.
20 . McNab JM and Boorman KN (2002) Poultry feedstuffs: supply, composition, and nutritive value. CAB International Publisher.
21 . Pourreza J, Samie AH and Rowghani E (2007) Effect of supplementation enzyme on nutrient digestibility and performance of broiler chicks fed diets containing triticale. International Journal of Poultry Science. 6 (2): 115-117.
22 . Ravindran V, Tilman ZV, Morel PCH, Ravindran G and Coles GD (2007) Influence of β-glucanase supplementation on the metabolisable energy and ileal nutrient digestibility of normal starch and waxy barleys for broiler chickens. Animal Feed Science and Technology. 134: 45-55.

23 . Rodriguez ML, Rebole A, Velasco S, Ortiz LT, Trevino J and Alzueta C (2012) Wheat- and barley-based diets with or without additives influence broiler chicken performance, nutrient digestibility and intestinal microflora. Journal of the Science of Food and Agriculture. 92: 184-190.

24 . Romero LF, Sands JS, Indrakumar SE, Plumstead PW, Dalsgaard S and Ravindran V (2014) Contribution of protein, starch, and fat to the apparent ileal digestible energy of corn- and wheat-based broiler diets in response to exogenous xylanase and amylase without or with protease. Poultry Science. 93: 2501-2513.
25 . Rutherfurd SM, Chung TK and Moughan PJ (2007) The effect of a commercial enzyme preparation on apparent metabolizable energy, the true ileal amino acid digestibility, and endogenous ileal lysine losses in broiler chickens. Poultry Science. 86: 665-672.
26 . SAS Institute (2003) SAS User’s Guide: Statistics. Version 9.1 ed. SAS Inst. Inc., Cary, NC.
27 . Steenfeldet S, Mullertz A and Jensen JF (1998) Enzyme supplementation of wheat based diets for broilers. Animal Feed Science and Technology. 75: 27-43.
28 . Van Beilen JB and Li Z (2002) Enzyme technology: an overview. Current Opinion in Biotechnology. 13: 338-344.
29 . Wang ZR, Qiao SY, Lu WQ and Li DF (2005) Effects of enzyme supplementation on performance, nutrient digestibility, gastrointestinal morphology and volatile fatty acid profiles in the hindgut of broilers fed wheat-based diets. Poultry Science. 84: 875-881.