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Effect of zinc nano-complex on bull semen quality and pregnancy outcome

Document Type : Research Paper

Authors

1 M.Sc., Department of Animal Science and Technology, Faculty of Agriculture and Natural Resources, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Assistant Professor, Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran

3 Assistant Professor, Department of Animal Science and Technology, Faculty of Agriculture and Natural Resources, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

4 Ph.D. Student of Animal Physiology, Faculty of Agriculture, University of Tabraiz, Iran

5 Ph.D., Nahadehaye Dami Jahed Company, Tehran, Iran

6 Associate Professor, Department of Animal Science and Technology, Faculty of Agriculture and Natural Resources, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

7 B.Sc., Sodour Ahrar Shargh Company, Tehran, Iran

Abstract

This study aimed to investigate the effect of different concentration of zinc nano-complex on bull spermatozoa quality and pregnancy outcome after freeze-thawing process. Ejaculates were collected from four Holstein bulls, twice a week during four weeks. Semen samples diluted with Bioxcell extender containing zero (Control), 10-6, 10-5, 10-4, 10-3 and 10-2 M of zinc nano-complex. The samples were then freezed-thawed after 72 hours. Motility of sperm was analyzed using computer assisted sperm analysis. The highest and lowest concentrations of zinc nano-complex along with control group were analyzed for the DNA fragmentation, malondialdehyde (MDA) concentration, mitochondrial activity, and pregnancy outcomes. The proportion of the total and progressive motile spermatozoa and the percentage of spermatozoa with fragmented DNA were not different among the groups. However, the groups containing 10-6 and 10-2 M zinc nano-complex had a lower level of MDA concentration and higher activity of mitochondria. Moreover, the pregnancy outcome was not affected by the treatment with zinc nano-complex. In conclusion, our data revealed that supplementation of bull semen Bioxcell extender with zinc nano-complex can decrease the level of MDA and improve mitochondrial activity, although such an improvement was not reflected in results of fertility rates.  

Keywords

References
1. رودباری ن، امیری‏نیا س، پریور ک و علیان ر (1392) جایگزینی آنتی‏بیوتیک با نانوذرات نقره در رقیق‏کننده‏های اسپرم گاو.تازه‏های بیوتکنولوژی سلولی - مولکولی. 10(3): 65-60.
 
2. Alvarez JG and Storey BT (1992) Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation. International Journal of Andrology. 13(3): 232-41.
3. Baumber J, Ball BA, Gravance CG, Medina V and Davies MCG (2000) The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential and membrane lipid peroxidation. International Journal of Andrology. 21(6): 895-902.
4. Bagchi D, Vuchetich PJ, Bagchi M, Tran MX, Krohn RL and Ray SD (1998) Protective effects of zinc salts on TPA-induced hepatic and brain lipid peroxidation, glutathione depletion, DNA damage and peritoneal macrophage activation in mice. General Pharmacology. 30(1): 43-50.
5. Bedwal RD and Bahuguna A (1994) Zinc, copper and selenium in reproduction. Experientia. 50(15): 626-40.
6. Breininger VE, Beorlegui NB, Oflaherti CM and Beconi MT (2005) Alpha tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen. Theriogenology. 63(8): 2126-35.
7. Chatterjee S and Gagnon C (2001) Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Molecular Reproduction and Development. 59(4): 451-8.
8. Chia SE, Onq CN, Chua LH, Ho LM and Tay SK (2000) Comparison of zinc conce ntration in blood and seminal plasma and various sperm parameters between fertile and infertile men. Journal of Andrology. 21(1): 53-7.
9. Costello LC, Guan Z, Franklin RB and Feng P (2004) Metallothionein can function as a chaperone for zinc uptake transport into prostate and liver mitochondria. Journal of Inorganic Biochemistry. 98(4): 664-666.
10. Edorh AP, Tachev K, Hadou T, Gbeassor M, Sanni A, Creppy EE, Le Faou A and Rihn Bh (2003) Magnesium content in seminal fluid as an indicator of chronic prostatitis. Cellular and molecular biology (Noisy-le-Grand, France). 49: 419-23.
11. Elgazar V and Razanov V (2005) Zinc-regulating proteins, ZnT-1, and Metallothionein I/II are present in different cell populations in the mouse testis. Histochemistry and Cytochemistry. 53(7): 905-912.
12. Endre L, Beck F and Parsad A (1999) The role of zinc in human health. Trace Elements in Experimental Medicine. 3(11): 333-375.
13. Fuse H, Kazama T, Ohta S and Fujiuchi Y (1999) Relationship between zinc concentrations in seminal plasma and various sperm parameters. International Urology and Nephrology. 31(3): 401-8.
14. Lasso JL, Noiles EE, Alvarez JG and Storey BT (1994) Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation. Journal of Andrology. 15(3): 255-65.
15. Hadwan MH, Almashhedy LA and Alsalman ARS (2012) Overal zinc supplementation restore high molecular weight seminal zinc binding protein to normal value in Iraqi infertile men. BMC Urology. 12: 32.
16. Mankad M, Sathawara NG, Doshi H, Saiyed HN and Kumar S (2006) Seminal plasma zinc concentration and α-glucosidase activity with respect to semen quality. Biological Trace Element Research. 110(2): 97-106.
17. Marmar JL, Katz S, Praiss DE and DeBenedictis TJ (1975) Semen zinc levels in infertile and post vasectomy patients and patients with prostatitis. Fertility and Sterility. 26(11): 1057-63.
18. Martins-Bessa A, Rocha A and Mayenco-Aguirre A (2009) Effects of taurine and hypotaurine supplementation and ionophore concentrations on post-thaw acrosome reaction of dog spermatozoa. Therioqenology. 71(2): 248-53.
19. Ménézo YJ, Hazout A, Panteix G, Robert F, Rollet J, Cohen-Bacrie P, Chapuis F, Clément P and Benkhalifa M (2007) Antioxidants to reduce sperm DNA fragmentation :an unexpected adverse effect. Reproductive BioMedicine Online. 14(4): 418-21.
20. Bucaka MN, Sarıozkanb S, Tuncera PB and Sakinc F (2010) The effect of antioxidants on post-thawed Angora goat (Capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities. Small Ruminant Research. 89(1): 24-30.
21. Powell SR (2000) The antioxidant properties of zinc. Nutrition. 130(5): 1447S-1454S.
22. Oteiza PI, Olin KL, Fraga CG and Keen CL (1995) Zinc deficiency causes oxidative damage to proteins, lipids and DNA in rat testes. Journal of Nutrition. 125(4): 823-9.
23. Salmani H, Nabi MM, Vaseghi-Dodaran H, Rahman MB, Mohammadi-Sangcheshmeh A, Shakeri M, Towhidi A, Zare-Shahneh A and Zhandi M (2013)  Effect of glutathione in soybean lecithin-based semen extender on goat semen quality after freeze–thawing. Small Ruminant Research. 112(1-3): 123-127.
24. Sonoko Y, Chiemi M, Kazuya K, Fritzie T, Tetsuro A, Shinsuke T and Takeshi M (2009) Zinc is an essential trace elemnt for spermatogenesis. Proceeding of the National Academy of Science. 106(26): 10589-10864.
25. Stoltenberg M, Sørensen MB, Danscher G, Juhl S, Andreasen A and Ernst E (1997) Autometallographic demonstration of zinc ion in rat sperm cell. Molecular Human Reproduction. 3(9): 763-767.
26.  Wang Y, Sharma RK and Agarwal A (1997)  Effect of cryopreservation and sperm concentration on lipid peroxidation in human semen. Urology. 50(3): 409-13.
27. Watson PF (2000) The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science. 60(1): 481-492.
28. Zago MP and Oteiza PI (2001) The antioxidant properties of zinc: interactions with iron and antioxidants. Free Radical Biology and Medicine. 31(2): 266-74.
29. Zanganeh Z, Zhandi M, Zare-Shahneha A, Najafi A, Nabi MM, and Mohammadi-Sangcheshmeh A (2003) Does rosemary aqueous extract improve buck semencryopreservation?. Small Ruminant Research. 114(1): 120-125.
Animal Production
Volume 18, Issue 1 - Serial Number 1
Spring
April 2016
Pages 173-181
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