Document Type : Research Paper
Authors
1 Ph.D. Student, Department of Animal Science, Faculty of Agriculture, University of Guilan, Rasht, Iran
2 Professor, Department of Animal Science, Faculty of Agriculture, University of Guilan, Rasht, Iran
3 Assistant Professor, Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Abstract
The Spermatogonial Stem Cells (SSCs) are founder of spermatogenesis and they are the only stem cells that can transmit genetic materials to offspring’s. This research was conducted to achieving a reliable origin of SSCs from the goat testicular tissues according to a four steps procedure; a) Collection and preparing testicular tissues, b) Two enzymatic digestions, c) Using mesh and cell suspension and d) Culture the isolated SSCs on goat embryonic fibroblasts. ِDistinct markers of pluripotency such as NANOG, SOX2and OCT4 were expressed in the isolated SSCs and also in these cells, nonspecific alkaline phosphatase test was positive. These cells used for co-culturing with GEF for several passages without differentiation or changing the shape. It was also found out that by using DMSO, these cells can be held in -70°C for one month. The result showed that the used procedure was efficient and the cells not only can express distinct markers of pluripotency but also can be culture and proliferate for long period of time without differentiations.
Keywords
-
Apont PM and DG De Rooij (2008) Biomanipulation of bovine spermatogonial stem cells. Animal Reprodution. 5(1/2): 16-22.
-
Borjigin Uynbilig, Ting Yun, Muren Herrid and Shorgan Bou (2012) Characterization and Isolation of Ovine Spermatogonial Stem Cells. Animal and Veterinary Advances. 11(8): 1242-1245.
-
Dobrinski I and JR Hill (2006) Male germ cell transplantation in livestock. Reproduction, Fertility and Development 18: 13-18.
-
Guilherme MJ Costa, Gleide F Avelar, Jose V Rezende-Neto, Paulo Henrique A Campos-Junior, Samyra MSN Lacerda, Bruno SC Andrade, Ralph Gruppi Thome, Marie-Claude Hofmann and Luiz R Franca (2012) Spermatogonial Stem Cell Markers and Niche in Equids. PLOS ONE. 7(8): 1-13.
-
Izadyar F, Spierenberg GT, Creemers LB, den Ouden K and DG De Rooij (2002) Isolation and purification of type A spermatogonia from the bovine testis. Reproduction. 124: 85-94.
-
Izadyar F, Matthijs-Rijsenbilt JJ, Krista Den Ouden, LB Creemrs, H Woelders and DG Rooij (2002) Development of a Cryopreservation Protocol for Type A Spermatogonia. Andrology. 23(4): 537-545.
-
Kolsa A, Misiakiewicz K, Marchlewicz M and Wiszniewska B (2012) The generating of spermatogonial stem cells and spermatogonia in mammals. Reproductive Biology. 12(1): 5-23.
-
Alison MR, Poulsom R, Forbes S and Wright NA (2002) An introduction to stem cells. Pathology 194(4): 419-23.
-
Lonnie D Russell, Robert A Ettlin, Amiya P, Sinha Hikim and Eric D Clegg (1993) Histological and Histopathological Evaluation of the Testis. Andrology. 16(1): 83-83.
-
Mayako F, Sung-Min K, Naojiro M, Masayasu Y and Hiroshi I (2011) Characterization and In Vitro culture of male germ cells from developing bovine testis. Reproduction and Development. 57(3): 355-364.
-
Mizrak SC and DG De Rooij (2008) Deriving multipotent stem cells from mouse spermatogonial stem cells: a new tool for developmental and clinical research. Development. 135: 2207-2213.
-
Nagano M, Mary R Avarbock, Efren B Leonida, Clayton J Brinster and Ralph L Brinster (1998) Culture of mouse spermatogonial stem cells. Tissue and Cell. 30(4): 389-397.
-
Nagano M, Patrizio P and Brinster RL (2002) Long-term survival of human spermatogonial stem cells in mouse testes. Fertil Steril. 78: 1225-33.
-
Oatley JM (2004) Development of Culture Methods for Spermatogonial stem cells and Ectopic Testicular Xenografting in the Bull. Washington State University. Department of Animal Sciences, Ph.D. Dissertation.
-
Oatley Jon, Mary R. Avarbock, Aino I Telaranta, Douglas T Fearon and Ralph L Brinster (2006) Identifying genes important for spermatogonial stem cell self-renewal and survival. PNAS. 103(25): 9524-9529.
-
Quesnell MM, Zhang Y, De Avila DM, Bertrand KP and Reeves JJ (2000) Immunization of male mice with luteinizing hormone-releasing hormone fusion proteins reduces testicular and accessory sex gland function. Biology of Reproduction. 63(1): 347-53.
-
Rooij DG De and Van Pelt AMM (2003) Spermatogonial Stem Cell Biology. Annual Review of Biomedical Sciences. 5: 105-114.
-
Sandeep Goel, Miki Sugimoto, Naojiro Minami, Masayasu Yamada, Shinichi Kume and Hiroshi Imai (2007) Identification, Isolation, and In Vitro Culture of Porcine Gonocytes. Biology of Reproduction. 77: 127-137.
-
Van Pelt Ans MM, Anna Rita Morena, Federica MF van Dissel-Emiliani, Carla Boitani, Ingrid C Gaemers, Dirk G de Rooij and Mario Stefanini (1996) Isolation of the Synchronized A Spermatogonia from Adult Vitamin A-Deficient Rat Testes. Biology of Reproduction. 55: 439-444.
-
Wyns Christine, Anne Van Langendonckt, Francois-Xavier Wese, Jacques Donnez and Mara Curaba (2008) Long-term spermatogonial survival in cryopreserved and xenografted immature human testicular tissue. Human Reproduction. 23(11): 2402-14.
-
Yan L, Lei L, Yang C, Gao Z, Lei A, Ma X and Dou Z (2008) Isolation and cultivation of goat embryo stem cells. Sheng Wu Gong Cheng Xue Bao. 24(9): 1670-6.
)