Study of beta-lactoglobulin genotypes in native and Holstein cattle of Kerman province

Mohammad Abadi, Mohammad Reza; Mohammadi, Akram

Authors

Abstract

Beta-lactoglobulin (LGB) is one of the proteins in the mammals’ milk synthesized by the epithelial cells of the mammary glands and affects the quality and coagulation of the milk. The aim of this study was to determine allele frequencies in LGB gene of Native and Holstein cattle in Kerman province using PCR-RFLP method. Genetic polymorphism, for 100 DNA samples in the locus was determined by digestion of PCR products with endonuclease HaeIII (LGB), followed by electrophoresis in agarose gel stained with ethidium bromide. Allele frequency for the A and B alleles in Holstein cattle was 0.62 and 0.38 and in Native cattle was 0.55 and 0.45, respectively. Average heterozygosity in Holstein and Native cattle was 0.47 and 0.46, respectively. Results indicated that the studied populations were not in Hardy-Weinberg equilibrium for the LGB locus.

Keywords

20.1001.1.20096776.1389.12.2.7.6

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Animal Production

Study of beta-lactoglobulin genotypes in native and Holstein cattle of Kerman province

References

References

Volume 12, Issue 2 - Serial Number 2
October 2010
Pages 61-67

Study of beta-lactoglobulin genotypes in native and Holstein cattle of Kerman province

References

References

Volume 12, Issue 2 - Serial Number 2October 2010Pages 61-67

Volume 12, Issue 2 - Serial Number 2
October 2010
Pages 61-67