Document Type : Research Paper

Authors

1 Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. E-mail: maededarkalh@gmail.com

2 Corresponding Author, Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. E-mail: z.ansari@sanru.ac.ir

3 Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. E-mail: dirandeh@gmail.com

4 Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. E-mail: Mo.kazemifard@gmail.com

10.22059/jap.2025.394259.623844

Abstract

Objective: Heat stress is major environmental stressors in the poultry industry that results in decreased feed intake, body weight, and carcass quality, decreased immune system activity, and subsequently increased flock mortality. This study was conducted to evaluate the effects of basil and parsley seed powders on growth performance, carcass traits, the relative gene expression of heat shock protein 70 (HSP70) in blood and interleukin-two (IL-2) in liver tissue, as some selected blood parameters of broiler chickens under heat-stress conditions.
Method: A total of 150 male Ross 308 broiler chicks were selected and weighed and then allocated into a completely randomized design with five treatments and three replicates (10 chickens per replicate). The experimental treatments included: 1) control (basal diet without basil or parsley seed powder); 2) basal diet supplemented with three g/kg basil seed powder; 3) basal diet supplemented with three g/kg parsley seed powder; 4) basal diet supplemented with 1.5 g/kg basil seed powder+ 1.5 g/kg parsley seed powder; and 5) basal diet supplemented with neomycin as a positive control (0.2% in the drinking water). Heat stress was induced by setting the temperature of the rearing house was set to 32°C for six hours from days 21 to 35. Performance traits (body weight gain, feed intake, and feed conversion ratio (FCR)) were measured throughout the trial, and  three birds per replicate (closest to the treatment mean body weight) were selected, weighed, and slaughtered at the end of the experiment to evaluate carcass characteristics, blood biochemical parameters (glucose, cholesterol, HDL, triglycerides), and the relative expression of target genes (HSP70 in blood and IL-2 in liver tissue).
Results: There was no significant differences infeed intake and weight gain; however among treatments, but the FCR during days 1 to 35 was lowest in the neomycin group (P<0.05). Treatments did not have a significant effect on blood biochemical parameters. The gizzard weight and jejunum length were significantly affected by  basil+ parsley seed powder compared with the control (P<0.05). The relative expression of the HSP70 gene in blood was significantly lower in the basil, parsley, and basil+parsley groups compared with the control and neomycin groups. The relative expression of the IL-2 gene in liver tissue was highest in the basil+ parsley group and lowest in the control group (P<0.05).
Conclusions: The supplementation with basil and parsley seed powders in the diet of broiler chickens under heat stress conditions can decrease the relative expression of the HSP70 gene and increase the relative expression of the IL-2 gene in the birds.

Keywords

موسوی علمداردهی، سید حامد؛ انصاری پیرسرائی، زربخت؛ دیرنده، عیسی و کاظمی‌فرد، محمد (1400). تأثیر استفاده از پودر و عصاره ریحان بر عملکرد، ویژگی‌های لاشه و بیان ژن پروتئین شوک گرمایی در جوجه‌های ‌گوشتی در شرایط تنش گرمایی. تولیدات دامی، 23(4)، 525-533.
 

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