Maryam Bazgiri; Mohammad taghi Beigi nassiri; Jamal Fayazi
Volume 19, Issue 1 , May 2017, , Pages 13-22
Abstract
This study was conducted to investigate the Single nucleotide polymorphism of STAT1 gene by PCR-RFLP technique and association of genotypes with milk production traits in Brown Swiss dairy cattle. The blood samples of ninety-eight cows were collected randomly from tail vein. The DNA was extracted with ...
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This study was conducted to investigate the Single nucleotide polymorphism of STAT1 gene by PCR-RFLP technique and association of genotypes with milk production traits in Brown Swiss dairy cattle. The blood samples of ninety-eight cows were collected randomly from tail vein. The DNA was extracted with Cinagene kit (PR881612C). Fragment of 314 bp in 3'UTR of STAT1 gene was amplified by PCR and digested by Pag1 restriction enzyme. Genotype frequencies were calculated by PopGene software (version 1.31). The SAS 9.1 software was used for statistical analysis and the mean of traits was compared by Duncan test. The genotypefrequencies of TT, TC and CC were 0.23, 0.25 and 0.52, respectively. The T and C allele frequency were 0.355 and 0.645, respectively. The expected heterozygosity was estimated 0.458. Result of Chi-square showed that this locus was not in the Hardy Weinberg equilibrium (P<0.05). The means of traits for milk, fat percent and fat yield, and protein yield were not significant but means of protein percent was significant (P<0.05). The mean of milk, fat and protein yield traits for CC genotype were more than two other genotypes. The results of present study showed that T allele is known as minor frequency allele and C allele have positive effect on milk, fat and protein yield traits.
Hengameh Vafaee; Mojtaba Hosseinpour Mashhadi2
Volume 18, Issue 3 , October 2016, , Pages 377-386
Abstract
This study was conducted to investigate the Single nucleotide polymorphism of STAT1 gene by PCR-RFLP technique and association of genotypes with milk production traits in Brown Swiss dairy cattle. The blood samples of ninety eight cows were collected randomly from tail vein. The DNA was extracted ...
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This study was conducted to investigate the Single nucleotide polymorphism of STAT1 gene by PCR-RFLP technique and association of genotypes with milk production traits in Brown Swiss dairy cattle. The blood samples of ninety eight cows were collected randomly from tail vein. The DNA was extracted with Cinagene kit (PR881612C). Fragment of 314 bp of STAT1 gene was amplified by PCR and digested by Pag1 restriction enzyme. Genotype frequencies were calculated by PopGene software (version 1.31). The SAS 9.1 software was used for statistical analysis and the mean of traits was compared by Duncan test. The genotype frequencies of TT, TC and CC were 0.23, 0.25 and 0.52 respectively. The T and C allele frequency were 0.355 and 0.645 respectively. The expected heterozygosity was estimated 0.458. Result of Chi-square showed that the population was not in the Hardy Weinberg equilibrium (P<0.05). The means of traits for milk, percent and yield of fat and yield of protein were not significant but means of percent of protein was significant(P<0.05). The mean of milk, fat and protein yield traits for CC genotype were more than two other genotypes. The results of present study show that the T allele is minor frequency allele and C allele has positive effect on milk, fat and protein yield traits.
Zohreh Shirmoradi; Abdol Reza Salehi; Rostam Pahlavan; Mohamad Reza Mollasalehi
Volume 12, Issue 2 , October 2010, , Pages 21-28
Abstract
In this study, the genetic and phenotypic trend of milk and fat production traits were estimated form 94121 cows’ first lactation records and that for age at first calving and calving interval traits were estimated from 41900 cows’ reproduction records. Data related to Iranian Holstein dairy ...
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In this study, the genetic and phenotypic trend of milk and fat production traits were estimated form 94121 cows’ first lactation records and that for age at first calving and calving interval traits were estimated from 41900 cows’ reproduction records. Data related to Iranian Holstein dairy cattle from 1995 to 2003. Data were analyzed, using univariate and mulitivariate animal model with genetic group effects. The heritability of milk yield, fat yield and fat percentage, age at first calving and calving interval were 0.29 (±0.01), 0.22 (±0.01), 0.38 (±0.01), 0.055 (±0.01) and 0.034 (±0.005), respectively. The linear regression of average breeding values and phenotypic values of cows for a trait on birth year provided an estimate of genetic and phenotypic trend, respectively. There genetic trend for milk yield, fat yield and Calving Interval were 53.5 kg, 1.07kg and 0.46 day, respectively. Their trend for fat percentage and Age at First Calving were -0.011% and -0.23 day, respectively. There was a positive phenotypic trend for milk yield, fat yield and fat percentage. This trend was negative for Age at First Calving and Calving Interval.