Zahra Alimohammadi; Hassan Shirzadi; Kamran Taherpour; Ali Khatibjoo
Volume 26, Issue 1 , March 2024, , Pages 73-86
Abstract
Introduction: With the increase of the population in the last century, the need for protein sources, especially protein with animal origin, has increased. One of the important and inexpensive sources of animal proteins is poultry products. However, many food-borne diseases that are among the most obvious ...
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Introduction: With the increase of the population in the last century, the need for protein sources, especially protein with animal origin, has increased. One of the important and inexpensive sources of animal proteins is poultry products. However, many food-borne diseases that are among the most obvious problems related to human health are transmitted to humans through poultry products. Generally, poultry are sensitive to pathogenic bacteria such as Clostridium, Salmonella, and Campylobacter. Campylobacter jejuni and Campylobacter coli are the main causes of bacterial enteritis in humans and account for about 90% and less than 10% of deaths in people with campylobacteriosis, respectively. Poultry farmers use antibiotic growth promoters to prevent the colonization of pathogenic bacteria, but their use has been banned in European Union since 2006 due to antibiotic resistance. However, in order to prevent the reduction of growth performance and mortality caused by the colonization of pathogenic bacteria in the gastrointestinal tract, it is necessary to introduce suitable alternatives. The antimicrobial potential of several medicinal plants, some plant-derived bioactive compoundsas well as some organic acids against a suspension of two Campylobacter serotypes (including 8 strains of C. jejuni and 3 strains of C. coli) has been investigated, under in vitro study. It has been revealed that oregano, rosemary, and cinnamon have a higher potential in reducing campylobacter colonization, among other phytobiotic compounds. Considering that under in vivo conditions, no research has been conducted on these medicinal plants with broiler chickens exposed to Campylobacter colonization. Therefore, this research was conducted with the aim of investigating the growth performance, excreta microbiota, intestinal morphology, and nutrient digestibility of broiler chickens challenged with C. jejuni and evaluating the potential of oregano, rosemary, and cinnamon in reducing the negative effects of this pathogen.
Material and Methods: A total of 192 one-day-old chicks were randomly allocated to 6 dietary treatments in a completely randomized design with 4 replicates of 8 birds. The dietary treatments were as follows: 1-negative control (NC; basal diet without additive and without of C. jejuni); 2-positive control (PC; basal diet without additives but challenged with C. jejuni); 3-basal diet + Erythromycin (55 mg/kg); 4-basal diet + oregano powder (3 g/kg); 5- basal diet + rosemary powder (3 g/kg) and 6-basal diet+cinnamon powder (3 g/kg). All chickens were orally gavaged once a day with a suspension of C. jejuni live culture (2×108 cfu/mL, 1 mL/bird) on days 21 to 25, with the exception of those fed the NC. Throughout the experimental period, the birds were fed ad libitum and had free access to water.
Results and Discussion: The results showed that the C. jejuni, while weakening performance during the growth period, caused an increase in campylobacter colonization and a decrease in lactobacilli counts of the excreta (P<0.05). The reason for the improvement of the microflora can be due to the presence of antibacterial compounds such as thymol, carvacrol, verbenone, and cinnamaldehyde in the composition of the mentioned medicinal plants. Campylobacter jejuni also decreased the digestibility of organic matter and led to a decrease in villous height, villous height to crypt depth ratio, and villi surface area in the jejunum (P<0.05). The reason for the improvement of the intestinal morphology by herbal additives can be due to the improvement of the microflora of the digestive tract. All the negative effects arising from C. jejuni were alleviated by dietary treatments containing feed additives (P<0.05).
Conclusion: In general, it can be concluded that oregano and rosemary have the potential to reduce the pathogenic effects of Campylobacter jejuni and can be used as suitable alternatives to antibiotics in feeding broilers.
Behnam Abbaspour; seyed davood sharifi; shokoufe ghazanfari; abdollah mohammadi sang cheshmeh; Shirin Honarbakhsh
Volume 21, Issue 2 , July 2019, , Pages 279-290
Abstract
This study aimed to evaluate the effect of L-arginine on qualitative characteristics and fatty acid profile of Ross 308 aged broiler breeder roosters’ sperm. Twelve breeder roosters at age of 52 week were used in a completely randomized design with three treatments and four replicates in each treatment ...
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This study aimed to evaluate the effect of L-arginine on qualitative characteristics and fatty acid profile of Ross 308 aged broiler breeder roosters’ sperm. Twelve breeder roosters at age of 52 week were used in a completely randomized design with three treatments and four replicates in each treatment for eight consecutive weeks. Experimental groups were consisting diets with levels of 0.52 (Ross recommendation 308), 0.68 and 0.83 present of arginine amino acids. Semen collection was performed every 14 days and in weeks 54, 56, 58 and 60 were tested. At the end of the trial, fatty acid profile of sperm was also evaluated. On week 56, semen volume in roosters with 0.68 present arginine was higher than other treatments (P<0.05). On week 60, levels of 0.52 and 0.68 percent arginine in semen volume, percentage of total and progressive motility were higher than the level of 0.83 percent of arginine (P<0.05). On week 58, the percentage of abnormal sperm were lower in 0.68 and 0.83 percent arginine treatment compared to 0.52 percent arginine (P<0.05). Semen concentration, sperm plasma membrane functionality and sperm fatty acid profile were not affected by treatments used in this study. It can be concluded that 0.68 percent of arginine (30% higher than recommendation) of diet improve some qualitative sperm parameters in aged broiler breeder roosters.
Hassan Shirzadi; Hossein Nasermanesh; Ali Khatibjoo; Kamran Taherpour; Mohammadi Akbari Gharaei
Volume 21, Issue 1 , April 2019, , Pages 151-163
Abstract
The effect of sweet wormwood essence (SWE) and Lactobacillus acidophilus on performance, ileal microbiology, ileal morphology and ileal nutrient digestibility of laying Japanese quails was investigated in 180 46-d-old female Japanese quail that were randomly allocated to 4 dietary treatments in a completely ...
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The effect of sweet wormwood essence (SWE) and Lactobacillus acidophilus on performance, ileal microbiology, ileal morphology and ileal nutrient digestibility of laying Japanese quails was investigated in 180 46-d-old female Japanese quail that were randomly allocated to 4 dietary treatments in a completely random design with 4 replicates and 9 birds per each. The experimental diets were as follows: basal diet as control group (without additive), basal diet plus Oxytetracycline (200mg/kg), basal diet plus SWE (250mg/kg), and basal diet plus lactobacillus acidophilus (4 cfu per kg diet). Results showed that the use of Lactobacillus acidophilus increased the egg mass per hen-housed compared to control and SWE treatments (P<0.05). The number of lactobacilli in the ileal digest was also significantly increased by Lactobacillus acidophilus and SWE treatments, when compared with control treatment (P<0.05). In addition, birds fed with diets containing Lactobacillus acidophilus and Oxytetracycline showed a significant increase in villous height and villous height:crypt depth, when compared with those on the control diet (P<0.05). It can be concluded that the use of Lactobacillus acidophilus as an alternative to antibiotics is more appropriate than SWE.
sadegh asadollahi
Volume 19, Issue 1 , May 2017, , Pages 99-115
Abstract
The effect of partial replacement of barley with sugar beet pulp (SBP) with and without roasted canola seeds (RCS) on the performance, morphology, VFA and digesta pH in the small intestine of finishing lambs was investigated. In this study 24 Arbia male lambs with average body weight of 23.7±2.5 ...
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The effect of partial replacement of barley with sugar beet pulp (SBP) with and without roasted canola seeds (RCS) on the performance, morphology, VFA and digesta pH in the small intestine of finishing lambs was investigated. In this study 24 Arbia male lambs with average body weight of 23.7±2.5 kg were used in a 2 × 2 factorial experiment over 84 days. Treatments were carbohydrate factor (barley or SBP) and roasted canola seed (with and without RCS). Partial replacement of barley with SBP significantly increased average daily gain (P < 0.05). Partial replacement of barley grain with SBP increased thickness of muscle, crypt depth and Brunner’s glands in duodenum and villus height, villous width, villus number, thickness of the epithelium, thickness of muscle, crypt depth and villous height to crypt depth in jejunum and villus height and thickness of muscle in ileum (p<0.05). Feeding lambs with diets containing RCS significantly increased Brunner’s glands,villus number and the thickness of the epithelium (P<0.05). Partial replacement of barely by SBP and adding RCS to diet increased significantly concentration individual VFA, total VFA and digesta pH in duodenum and jejunum. Based on the results, the performance of lambs improved as a result of partial replacement of barley with SBP and addition RCS to diets with high concentrate due to changes in the density of volatile fatty acids, digestive fluid pH of the small intestine and the development of the small intestine tissue morphology.
behrooz shokrani; morteza mehri; amir fattah; mohsen sharafi; fatemeh shirmohammad
Volume 18, Issue 4 , December 2016, , Pages 853-865
Abstract
Effects of dietary rosemary (Rosmarinus officinalis) essential oil were evaluated on the semen quality of broiler breeder rooster using sixteen 24-wk Ross 308 male birds. Experimental groups were consisting of four treatments (0, 50, 100 and 200 mg rosemary essential oil/kg of feed) with four replicates ...
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Effects of dietary rosemary (Rosmarinus officinalis) essential oil were evaluated on the semen quality of broiler breeder rooster using sixteen 24-wk Ross 308 male birds. Experimental groups were consisting of four treatments (0, 50, 100 and 200 mg rosemary essential oil/kg of feed) with four replicates per each group. Semen samples were collected on days 0, 14, 28, 42 and 56 of experiment. Results showed that 100mg rosemary significantly improved several traits of sperm such as Amplitude of Lateral Head Displacement (ALH), Average Path Velocity (VAP), and Straight Line Velocity (VSL) on days 42 and 56, and sperm Membrane Integrity (MI), sperm viability, Linearity (LIN) and Total Motility (TM) on day 56 (P<0.05) and significantly decreased dead sperms (P<0.05). However, sperm concentration, apoptosis and morphological changes, Sperm Track Straightness (STR), Curvilinear Velocity (VCL), Progressive motility (PM) and malondialdehyde (MDA) level were not affected by experimental treatment. It can be concluded that 100 mg rosemary essential oil/kg of diet could be efficient for improvement of some qualitative sperm parameters.
Mehdi Abediny Sanigy; farid shariatmadari; Mohammad Amir Karimi Torshizi
Volume 13, Issue 2 , March 2012, , Pages 19-27
Abstract
400 one day-old broiler chicks were allocated on a randomized complete design experiment with five treatments consisting of four replications for 42 days. Experiment treatments were included: 1 . based diet consist 20 percent barley (barley control), 2 . barley control + 0.1 percent of mixed essential ...
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400 one day-old broiler chicks were allocated on a randomized complete design experiment with five treatments consisting of four replications for 42 days. Experiment treatments were included: 1 . based diet consist 20 percent barley (barley control), 2 . barley control + 0.1 percent of mixed essential oil (Antibiofin), 3 . barley control + 0.2 percent organic acid, 4 . barley control + 15 ppm of antibiotic (virginiamycin) and 5 . corn-soybean based diet as corn control. In addition, Basal diet supplemented with commercial enzyme preparation. There was significant difference within treatments for 42 age's body weight and FCR. No significant differences in FI were noted among treatment during experiment. Antibody titer against (SRBC) among groups in secondly immuno response had a significant effect (P< 0.05). The villus height of deudenum and Jejunum was significant differences among the treatment groups. It can be concluded that by using additives such as medicinal plant and organic acids in diets containing barley, can be achieved to similar performance of corn based diets.