Ramin Farhadi; Abbas Farshad; Jalal Rostamzadeh; Abouzar Najafi
Abstract
The present study was conducted in order to use different levels of curcumin in the diluting sperm epididymis of Shal breed rams during storage at five degrees Celsius in the form of a completely randomized design with four treatments in six replications. Sperm samples were extracted from the testicular ...
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The present study was conducted in order to use different levels of curcumin in the diluting sperm epididymis of Shal breed rams during storage at five degrees Celsius in the form of a completely randomized design with four treatments in six replications. Sperm samples were extracted from the testicular tissue and used in the Tris-egg yolk diluent. Different concentrations of curcumin including 0, 10, 25 and 50 μM were added to the diluents. The samples were gradually cooled at 5°C and after stabilization at this temperature, in 6, 12, 24 and 48 hours, the parameters of total motility, progressive motility, viability, membrane integrity and the percentage of sperm abnormalities were evaluated. The results showed that the use of 25μM curcumin improved the parameters of total motility, progressive motility, viability and membrane integrity only at 6 and 12 hours, but no significant difference was observed at 24 and 48 hours compared to other treatment groups. Regarding sperm abnormalities, no significant difference was observed between the treatments in all the examined times. The results of this study indicate that using a concentration of 25μM curcumin can lead to the improvement of ram epididymal sperm quality in the initial periods of storage, but it loses its effect in longer periods of time. Therefore, it is recommended to use nanotechnology methods to improve the effectiveness of curcumin, especially during long-term storage of ram sperm.
Amin Kazemizadeh; Ahmad Zare Shahneh; Ali Reza Yousefi; Hasan Mehrabani Yeganeh; Zarbakht Ansari Pirsaraei
Abstract
The present study was conducted to investigate the effect of dietary Curcumin supplementation on histological parameters of testis in aged Ross 308 broiler breeder roosters. A total of twelve 48-week old broiler breeder roosters in a completely randomized design were randomly assigned to four treatments ...
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The present study was conducted to investigate the effect of dietary Curcumin supplementation on histological parameters of testis in aged Ross 308 broiler breeder roosters. A total of twelve 48-week old broiler breeder roosters in a completely randomized design were randomly assigned to four treatments and three replicates during a 13 weeks of experimental period. Treatments included no dietary Curcumin supplementation (control group), and daily supplementation of 10, 20, and 30 mg Curcumin/birds as mixed in the basal diet. At the end of the experimental period, all of the roosters were slaughtered, and testis tissue samples were collected. Testicular weight was higher in the roosters that daily received 30 mg Curcumin compared with the control group (P<0.05). Dietary supplementation of 20 and 30 mg Curcumin/day increased the diameter of seminiferous tubule compared to the control group (P<0.05). Seminiferous epithelium thickness was dose-dependently increased in Curcumin-supplemented birds compared to the control group (P<0.05). The number of spermatogonia cells was increased in all treatment groups compared to the control group (P<0.05). The number of Leydig cells was also increased in roosters received 20 and 20 mg Curcumin/day compared to the control birds (P<0.05). However, treatments did not affect the number of testis blood vessels (P>0.05). According to the results of the present study, dietary supplementation of 30 mg Curcumin/day/bird improves testis histological parameters in aged broiler breeder roosters.