Mohammad Abdoli; Mohammad Bagher Zandi; Taher harkinezhad; Masoud Kahlili
Volume 23, Issue 2 , July 2021, , Pages 155-163
Abstract
The aim of this study was to investigate the genetic structure of Iranian native horse breeds to compare genetic diversity and understanding the relationships between the populations using 11 ISAG microsatellite markers. For this reason, 565 samples of the Iranian Equestrian database from different ages ...
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The aim of this study was to investigate the genetic structure of Iranian native horse breeds to compare genetic diversity and understanding the relationships between the populations using 11 ISAG microsatellite markers. For this reason, 565 samples of the Iranian Equestrian database from different ages including the Iranian Arab Asil, Caspian, Darehshouri, Kurdish and Turkmen and 113 samples were used for each breed. The number of observed alleles for each locus was 7 to 19 alleles with an average of 10.81 alleles, and it was 19 for ASB17 and 7 for HTG4 locus with the highest and lowest observed alleles ranking respectively. The average observed heterozygosity from the largest to the lowest rank was, Turkmen (0.68±0.11), Caspian (0.67±0.07), Kurdish (0.66±0.06) Darehshouri (0.65±0.07), and Arab Asil (0.62±0.08). Population structure analysis with UPGMA method showed that Caspian and Kurdish populations were grouped as a unit cluster while the other populations grouped as a separate cluster. These results confirmed this hypothesis that the Caspian and Kurdish populations are close to the Nisa horses. In general, the results of this study indicate that the Iranian native horses have got a high genetic diversity, despite of populations have genetic similarity and the other hand genetic clustering of the populations is consistent with their geographic distances. The result of this study shows that the ISAG microsatellite markers are polymorphic and have more efficiency for assignment genetic diversity and genetic structure analysis of Iranian native horse breeds.
Nejat Badbarin; nymat hydaeat
Volume 18, Issue 4 , December 2016, , Pages 661-670
Abstract
The aim of this study was to map body weight QTLs with some microsatellite markers on chromosome 2 of Markhoz goats. For this purpose a total of 255 offspring including 129 male and 126 female from 8 half-sib sire families were genotyped for 6 microsatellite markers on chromosomes 2. Quantitative traits ...
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The aim of this study was to map body weight QTLs with some microsatellite markers on chromosome 2 of Markhoz goats. For this purpose a total of 255 offspring including 129 male and 126 female from 8 half-sib sire families were genotyped for 6 microsatellite markers on chromosomes 2. Quantitative traits were included weights at birth, 4 months, 6 months, 9 months and 12 months of age. Significant QTL effect was tested using the least squared regression approach using GridQTL (V3.3.0) software. In this study, one putative QTL were detected for weaning weight at 153 cM on chromosome 2 (P<0.01). QTL effect was 4.99 in units of residual standard deviation. The position of this QTL was between the IDVGA64 and OarFCB011 and its distance was measured 10 cM of IDVGA64 markers. In the present study any pleiotropic effect of this QTL with other traits were not recognized. It is expected that future genome wide scan with larger individual per family will help identify pleotropic effects and other QTLs associated with these traits and will provide useful markers for marker-assisted selection of such important traits. According to ensure high confidence interval (CM = 25 cM) Offer is not valid candidate genes for QTL set and is recommended for accurate determination of the QTL in future studies of more offspring in the family are applied.
Hasan Moradian; Ali Esmailizadeh; Mohammadreza Mohammadabadi
Volume 15, Issue 2 , October 2013, , Pages 89-99
Abstract
The purpose of this study was to identify genomic regions of quantitative trait loci (QTL), affectingcarcass traits on chromosome 1 in an F2 population of Japanese quail. For this purpose, a three-generationresource population was developed by using two distinct Japanese quail strains, wild (meat type) ...
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The purpose of this study was to identify genomic regions of quantitative trait loci (QTL), affectingcarcass traits on chromosome 1 in an F2 population of Japanese quail. For this purpose, a three-generationresource population was developed by using two distinct Japanese quail strains, wild (meat type) andwhite (layer type). Eight pairs of white and wild birds were crossed reciprocally and 34 F1 birds wereproduced. The F1 birds were intercrossed to generate 422 F2 offspring. Phenotypic data including weightof carcass parts were collected on F2 birds. All of the animals from three generations (472 birds) weregenotyped for eight microsatellite markers on chromosome 1. QTL analysis was performed with leastsquares interval mapping method fitting three various statistical models. Significant QTL were identifiedfor breast weight, carcass weight, head weight and percentage of breast. There was also evidence forimprinted QTL affecting breast weight, a carcass part of high economic value, on chromosome 1. Theproportion of the F2 phenotypic variation explained by the significant additive, dominance and imprintedQTL effects ranged from 1.8 to 2.3, 1.2 to 3.3 and 0.5 to 2.2 percent, respectively.